In:
Current Protocols in Cell Biology, Wiley, Vol. 50, No. 1 ( 2011-03)
Abstract:
We present a protocol for in vitro production of recombinant lentiviruses using COS‐1 African green monkey kidney epithelial cells and HEK293T human embryonic kidney epithelial cells as packaging cells. COS‐1 and HEK293T express SV40 large T antigen, amplifying transfected circular plasmids harboring SV40 replication origin. Support protocols for evaluation of transfection efficiency by in situ β‐galactosidase enzyme activity assay and titer of infection‐capable virions are also provided. Advantages of using COS‐1 packaging cells over the standard HEK293T cells for contamination‐sensitive applications or automated processing are discussed. Curr. Protoc. Cell Biol . 50:26.7.1‐26.7.15. © 2011 by John Wiley & Sons, Inc.
Type of Medium:
Online Resource
ISSN:
1934-2500
,
1934-2616
DOI:
10.1002/0471143030.2011.50.issue-1
DOI:
10.1002/0471143030.cb2607s50
Language:
English
Publisher:
Wiley
Publication Date:
2011
detail.hit.zdb_id:
2179048-6
Permalink