In:
Cell Proliferation, Wiley, Vol. 50, No. 4 ( 2017-08)
Abstract:
Neural stem cells ( NSC s) are self‐renewing, undifferentiated and multipotent precursors that can generate neuronal and glial lineages. Micro RNA s (mi RNA s) are small non‐coding RNA s that act crucial roles in cell proliferation, differentiation and migration. However, the role of miR‐1297 in the development of NSC s is still unknown. Materials and methods Primary NSC s were isolated from rat's embryos. The expression of miR‐1297 and Hes1 were measured by qRT ‐ PCR . Western blot was performed to detect the protein expression of Hes1, β‐tubulin‐ III and GFAP . Results We showed that miR‐1297 expression was upregulated during NSC differentiation, while the expression of Hes1 was decreased during NSC differentiation. Elevated expression of miR‐1297 promoted the NSC s viability and increased the formation of NSC s to neurospheres. Ecoptic expression of miR‐1297 promoted β‐tubulin‐ III expression in the NSC s. Overexpression of miR‐1297 decreased GFAP expression in the NSC s. Furthermore, we demonstrated that miR‐1297 regulated NSC s viability and differentiation by directly targeting Hes1. Overexpression of miR‐1297 suppressed Hes1 expression in the NSC s. Conclusions These results suggested that miR‐1297 played an important role in NSC s viability and differentiation through inhibiting Hes1 expression.
Type of Medium:
Online Resource
ISSN:
0960-7722
,
1365-2184
DOI:
10.1111/cpr.2017.50.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2019986-7
SSG:
12
Permalink
