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  • 1
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    Increased Sensitivity of Ascorbate Detection by Mediated Oxidation in Confined Electrochemical Cells
    Wiley ; 2023
    In:  Electroanalysis Vol. 35, No. 1 ( 2023-01)
    Details
    In: Electroanalysis, Wiley, Vol. 35, No. 1 ( 2023-01)
    Abstract: In this work, the EC’ mechanism involving ascorbate (AA − ) and the oxidized form of ferrocenemethanol (FcMeOH) was explored as an analytical strategy to monitor AA − at low concentration levels. The feasibility of this approach was investigated at different mass transport regimes utilizing macro‐ (glassy carbon) and ultramicroelectrodes (5 μm radius carbon disk). Cyclic voltammograms (CV) were recorded in acetate buffer solution (pH 3.7) using a glassy carbon electrode, and an expressive increase in the anodic peak current (and decrease in the cathodic peak) was noticed in the CV in a solution containing both FcMeOH and ascorbate, confirming the presence of an electrocatalytic process (EC’). The current increase was more pronounced when the reactants and products were confined in a thin solution layer, which was created by approaching an ultramicroelectrode close to an insulator surface. At optimized experimental conditions, a correlation between ascorbate concentration and the steady‐state current measured at the ultramicroelectrode was established, allowing the development of an analytical method for ascorbate detection in the micromolar range. The proposed approach was used to quantify ascorbate in a commercial juice sample.
    Type of Medium: Online Resource
    ISSN: 1040-0397 , 1521-4109
    URL: Issue
    URL: Article
    DOI: 10.1002/elan.v35.1
    DOI: 10.1002/elan.202100696
    Language: English
    Publisher: Wiley
    Publication Date: 2023
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  • 2
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    Brazilian Flora 2020: Leveraging the power of a collaborative scientific network
    Wiley ; 2022
    In:  TAXON Vol. 71, No. 1 ( 2022-02), p. 178-198
    Details
    In: TAXON, Wiley, Vol. 71, No. 1 ( 2022-02), p. 178-198
    Abstract: The shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiversity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxonomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world's known plant species than any other country. Despite that, Flora Brasiliensis , concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world's most biodiverse countries. We further identify collection gaps and summarize future goals that extend beyond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still unequally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the country. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora.
    Type of Medium: Online Resource
    ISSN: 0040-0262 , 1996-8175
    URL: Issue
    URL: Article
    DOI: 10.1002/tax.v71.1
    DOI: 10.1002/tax.12640
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2081189-5
    detail.hit.zdb_id: 204216-2
    SSG: 12
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  • 3
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    NEOTROPICAL ALIEN MAMMALS: a data set of occurrence and abundance of alien mammals in the Neotropics
    Wiley ; 2020
    In:  Ecology Vol. 101, No. 11 ( 2020-11)
    Details
    In: Ecology, Wiley, Vol. 101, No. 11 ( 2020-11)
    Abstract: Biological invasion is one of the main threats to native biodiversity. For a species to become invasive, it must be voluntarily or involuntarily introduced by humans into a nonnative habitat. Mammals were among first taxa to be introduced worldwide for game, meat, and labor, yet the number of species introduced in the Neotropics remains unknown. In this data set, we make available occurrence and abundance data on mammal species that (1) transposed a geographical barrier and (2) were voluntarily or involuntarily introduced by humans into the Neotropics. Our data set is composed of 73,738 historical and current georeferenced records on alien mammal species of which around 96% correspond to occurrence data on 77 species belonging to eight orders and 26 families. Data cover 26 continental countries in the Neotropics, ranging from Mexico and its frontier regions (southern Florida and coastal‐central Florida in the southeast United States) to Argentina, Paraguay, Chile, and Uruguay, and the 13 countries of Caribbean islands. Our data set also includes neotropical species (e.g., Callithrix sp., Myocastor coypus , Nasua nasua) considered alien in particular areas of Neotropics. The most numerous species in terms of records are from Bos sp. ( n = 37,782), Sus scrofa ( n = 6,730), and Canis familiaris ( n = 10,084); 17 species were represented by only one record (e.g., S yncerus caffer, Cervus timorensis, Cervus unicolor, Canis latrans ). Primates have the highest number of species in the data set ( n = 20 species), partly because of uncertainties regarding taxonomic identification of the genera Callithrix, which includes the species Callithrix aurita, Callithrix flaviceps, Callithrix geoffroyi, Callithrix jacchus, Callithrix kuhlii, Callithrix penicillata , and their hybrids. This unique data set will be a valuable source of information on invasion risk assessments, biodiversity redistribution and conservation‐related research. There are no copyright restrictions. Please cite this data paper when using the data in publications. We also request that researchers and teachers inform us on how they are using the data.
    Type of Medium: Online Resource
    ISSN: 0012-9658 , 1939-9170
    URL: Issue
    URL: Article
    DOI: 10.1002/ecy.v101.11
    DOI: 10.1002/ecy.3115
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2020
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  • 4
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    NEOTROPICAL XENARTHRANS: a data set of occurrence of xenarthran species in the Neotropics
    Wiley ; 2019
    In:  Ecology Vol. 100, No. 7 ( 2019-07)
    Details
    In: Ecology, Wiley, Vol. 100, No. 7 ( 2019-07)
    Abstract: Xenarthrans—anteaters, sloths, and armadillos—have essential functions for ecosystem maintenance, such as insect control and nutrient cycling, playing key roles as ecosystem engineers. Because of habitat loss and fragmentation, hunting pressure, and conflicts with domestic dogs, these species have been threatened locally, regionally, or even across their full distribution ranges. The Neotropics harbor 21 species of armadillos, 10 anteaters, and 6 sloths. Our data set includes the families Chlamyphoridae (13), Dasypodidae (7), Myrmecophagidae (3), Bradypodidae (4), and Megalonychidae (2). We have no occurrence data on Dasypus pilosus (Dasypodidae). Regarding Cyclopedidae, until recently, only one species was recognized, but new genetic studies have revealed that the group is represented by seven species. In this data paper, we compiled a total of 42,528 records of 31 species, represented by occurrence and quantitative data, totaling 24,847 unique georeferenced records. The geographic range is from the southern United States, Mexico, and Caribbean countries at the northern portion of the Neotropics, to the austral distribution in Argentina, Paraguay, Chile, and Uruguay. Regarding anteaters, Myrmecophaga tridactyla has the most records ( n  = 5,941), and Cyclopes sp. have the fewest ( n  = 240). The armadillo species with the most data is Dasypus novemcinctus ( n  = 11,588), and the fewest data are recorded for Calyptophractus retusus ( n  = 33). With regard to sloth species, Bradypus variegatus has the most records ( n  = 962), and Bradypus pygmaeus has the fewest ( n  = 12). Our main objective with Neotropical Xenarthrans is to make occurrence and quantitative data available to facilitate more ecological research, particularly if we integrate the xenarthran data with other data sets of Neotropical Series that will become available very soon (i.e., Neotropical Carnivores, Neotropical Invasive Mammals, and Neotropical Hunters and Dogs). Therefore, studies on trophic cascades, hunting pressure, habitat loss, fragmentation effects, species invasion, and climate change effects will be possible with the Neotropical Xenarthrans data set. Please cite this data paper when using its data in publications. We also request that researchers and teachers inform us of how they are using these data.
    Type of Medium: Online Resource
    ISSN: 0012-9658 , 1939-9170
    URL: Issue
    URL: Article
    DOI: 10.1002/ecy.2019.100.issue-7
    DOI: 10.1002/ecy.2663
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1797-8
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  • 5
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    ATLANTIC ANTS: a data set of ants in Atlantic Forests of South America
    Wiley ; 2022
    In:  Ecology Vol. 103, No. 2 ( 2022-02)
    Details
    In: Ecology, Wiley, Vol. 103, No. 2 ( 2022-02)
    Abstract: Ants, an ecologically successful and numerically dominant group of animals, play key ecological roles as soil engineers, predators, nutrient recyclers, and regulators of plant growth and reproduction in most terrestrial ecosystems. Further, ants are widely used as bioindicators of the ecological impact of land use. We gathered information of ant species in the Atlantic Forest of South America. The ATLANTIC ANTS data set, which is part of the ATLANTIC SERIES data papers, is a compilation of ant records from collections (18,713 records), unpublished data (29,651 records), and published sources (106,910 records; 1,059 references), including papers, theses, dissertations, and book chapters published from 1886 to 2020. In total, the data set contains 153,818 ant records from 7,636 study locations in the Atlantic Forest, representing 10 subfamilies, 99 genera, 1,114 ant species identified with updated taxonomic certainty, and 2,235 morphospecies codes. Our data set reflects the heterogeneity in ant records, which include ants sampled at the beginning of the taxonomic history of myrmecology (the 19th and 20th centuries) and more recent ant surveys designed to address specific questions in ecology and biology. The data set can be used by researchers to develop strategies to deal with different macroecological and region‐wide questions, focusing on assemblages, species occurrences, and distribution patterns. Furthermore, the data can be used to assess the consequences of changes in land use in the Atlantic Forest on different ecological processes. No copyright restrictions apply to the use of this data set, but we request that authors cite this data paper when using these data in publications or teaching events.
    Type of Medium: Online Resource
    ISSN: 0012-9658 , 1939-9170
    URL: Issue
    URL: Article
    DOI: 10.1002/ecy.v103.2
    DOI: 10.1002/ecy.3580
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2022
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  • 6
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    ATLANTIC ‐ CAMTRAPS : a dataset of medium and large terrestrial mammal communities in the Atlantic Forest of South America
    Wiley ; 2017
    In:  Ecology Vol. 98, No. 11 ( 2017-11), p. 2979-2979
    Details
    In: Ecology, Wiley, Vol. 98, No. 11 ( 2017-11), p. 2979-2979
    Abstract: Our understanding of mammal ecology has always been hindered by the difficulties of observing species in closed tropical forests. Camera trapping has become a major advance for monitoring terrestrial mammals in biodiversity rich ecosystems. Here we compiled one of the largest datasets of inventories of terrestrial mammal communities for the Neotropical region based on camera trapping studies. The dataset comprises 170 surveys of medium to large terrestrial mammals using camera traps conducted in 144 areas by 74 studies, covering six vegetation types of tropical and subtropical Atlantic Forest of South America (Brazil and Argentina), and present data on species composition and richness. The complete dataset comprises 53,438 independent records of 83 species of mammals, includes 10 species of marsupials, 15 rodents, 20 carnivores, eight ungulates and six armadillos. Species richness averaged 13 species (±6.07 SD ) per site. Only six species occurred in more than 50% of the sites: the domestic dog Canis familiaris , crab‐eating fox Cerdocyon thous , tayra Eira barbara , south American coati Nasua nasua , crab‐eating raccoon Procyon cancrivorus and the nine‐banded armadillo Dasypus novemcinctus . The information contained in this dataset can be used to understand macroecological patterns of biodiversity, community, and population structure, but also to evaluate the ecological consequences of fragmentation, defaunation, and trophic interactions.
    Type of Medium: Online Resource
    ISSN: 0012-9658 , 1939-9170
    URL: Issue
    URL: Article
    DOI: 10.1002/ecy.2017.98.issue-11
    DOI: 10.1002/ecy.1998
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 1797-8
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  • 7
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    A new subfamily classification of the Leguminosae based on a taxonomically comprehensive phylogeny: The Legume Phylogeny Working Group (LPWG)
    Wiley ; 2017
    In:  TAXON Vol. 66, No. 1 ( 2017-02), p. 44-77
    Details
    In: TAXON, Wiley, Vol. 66, No. 1 ( 2017-02), p. 44-77
    Abstract: The classification of the legume family proposed here addresses the long‐known non‐monophyly of the traditionally recognised subfamily Caesalpinioideae, by recognising six robustly supported monophyletic subfamilies. This new classification uses as its framework the most comprehensive phylogenetic analyses of legumes to date, based on plastid matK gene sequences, and including near‐complete sampling of genera (698 of the currently recognised 765 genera) and ca. 20% (3696) of known species. The matK gene region has been the most widely sequenced across the legumes, and in most legume lineages, this gene region is sufficiently variable to yield well‐supported clades. This analysis resolves the same major clades as in other phylogenies of whole plastid and nuclear gene sets (with much sparser taxon sampling). Our analysis improves upon previous studies that have used large phylogenies of the Leguminosae for addressing evolutionary questions, because it maximises generic sampling and provides a phylogenetic tree that is based on a fully curated set of sequences that are vouchered and taxonomically validated. The phylogenetic trees obtained and the underlying data are available to browse and download, facilitating subsequent analyses that require evolutionary trees. Here we propose a new community‐endorsed classification of the family that reflects the phylogenetic structure that is consistently resolved and recognises six subfamilies in Leguminosae: a recircumscribed Caesalpinioideae DC., Cercidoideae Legume Phylogeny Working Group (stat. nov.), Detarioideae Burmeist., Dialioideae Legume Phylogeny Working Group (stat. nov.), Duparquetioideae Legume Phylogeny Working Group (stat. nov.), and Papilionoideae DC. The traditionally recognised subfamily Mimosoideae is a distinct clade nested within the recircumscribed Caesalpinioideae and is referred to informally as the mimosoid clade pending a forthcoming formal tribal and/or clade‐based classification of the new Caesalpinioideae. We provide a key for subfamily identification, descriptions with diagnostic charactertistics for the subfamilies, figures illustrating their floral and fruit diversity, and lists of genera by subfamily. This new classification of Leguminosae represents a consensus view of the international legume systematics community; it invokes both compromise and practicality of use.
    Type of Medium: Online Resource
    ISSN: 0040-0262 , 1996-8175
    URL: Article
    DOI: 10.12705/661.3
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2081189-5
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  • 8
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    Yellow fever and orthotopic liver transplantation: new insights from the autopsy room for an old but re‐emerging disease
    Wiley ; 2019
    In:  Histopathology Vol. 75, No. 5 ( 2019-11), p. 638-648
    Details
    In: Histopathology, Wiley, Vol. 75, No. 5 ( 2019-11), p. 638-648
    Abstract: The clinical spectrum of yellow fever (YF) ranges from asymptomatic to fulminant hepatitis. During the sylvatic YF epidemic in the metropolitan area of São Paulo, Brazil in 2018, seven orthotopic liver transplantations (OLTs) were performed in our institution to treat fulminant YF hepatitis. Three patients recovered, while four patients died following OLT. The autopsy findings of all these cases are presented herein as the first description of YF in transplanted patients. Methods and results All patients were men, aged 16–40 years, without vaccination to YF virus (YFV). All organs were examined, with tissue sampling for histopathological analysis. Detection of YF virus antigens (YFV Ag) was performed with two primary antibodies (mouse polyclonal anti‐YFV antibody directed to wild strain and a goat anti‐YF virus antibody), and RT–PCR assays were utilised to detect YFV‐RNA. All the cases depicted typical findings of YF hepatitis in the engrafted liver. The main extrahepatic findings were cerebral oedema, pulmonary haemorrhage, pneumonia, acute tubular necrosis and ischaemic/reperfusion pancreatitis. Of the four cases, the YVF Ag was detected in the heart in one case, liver and testis in three cases, and the kidney and spleen in all four cases. All four cases had YF virus RNA detected by RT–PCR in the liver and in other organs. Conclusions Infection of the engrafted liver and other organs by YFV, possibly combined with major ischaemic systemic lesions, may have led to the death of four of the seven patients undergoing OLT.
    Type of Medium: Online Resource
    ISSN: 0309-0167 , 1365-2559
    URL: Issue
    URL: Article
    DOI: 10.1111/his.v75.5
    DOI: 10.1111/his.13904
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2006447-0
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  • 9
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    Sensitive Electroanalytical Detection on GCE: the Case of Lipoic Acid and its Interaction with N ‐acetylcysteine and Glutathione
    Wiley ; 2016
    In:  Electroanalysis Vol. 28, No. 11 ( 2016-11), p. 2818-2826
    Details
    In: Electroanalysis, Wiley, Vol. 28, No. 11 ( 2016-11), p. 2818-2826
    Abstract: A simple and sensitive electrochemical method was developed for the determination of N ‐Acetylcysteine (NAC) and Glutathione (GSH), on glassy carbon electrode. The results indicate that α‐lipoic acid (LipS 2 ) is oxidized at the electrode, but is regenerated by thiols, suggesting a redox catalytic effect. Under optimized conditions, the analytical curves for NAC and GSH were obtained in the range of 1 up to 10 µM and 1 up to 60 µM, respectively. The quantification can be performed in a single assay with high sensitivity, reproducibility and low detection limit of 0.27 and 0.67 µM for NAC and GSH, respectively. This method was successfully applied to the determination of NAC and GSH in artificial saliva and human urine samples, with excellent recovery results.
    Type of Medium: Online Resource
    ISSN: 1040-0397 , 1521-4109
    URL: Issue
    URL: Article
    DOI: 10.1002/elan.v28.11
    DOI: 10.1002/elan.201600205
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 1483564-2
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  • 10
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    Re‐ensiling effects on sorghum silage quality, methane emission and sheep efficiency in tropical climate
    Wiley ; 2021
    In:  Grass and Forage Science Vol. 76, No. 3 ( 2021-09), p. 440-450
    Details
    In: Grass and Forage Science, Wiley, Vol. 76, No. 3 ( 2021-09), p. 440-450
    Abstract: The objective of the present study was to evaluate whether sorghum silage exposure to air for 8, 16 or 24 hr before re‐ensiling reduces silage quality and animal feed efficiency. Sorghum was ensiled in 52 metal drums (13 for each exposure time) with 200‐L capacity, and after 112 days, 39 drums were opened and silage was exposed to air for 8, 16 or 24 hr. Thirteen silos were not opened and considered control treatment. Subsequently, silage was re‐ensiled, and after 56 days, all silos were opened to determine silage quality variables. In addition, the silage was offered to sheep to determine animal feed efficiency. The experimental design was completely randomized with five replications per treatment, and orthogonal polynomials were used to determine linear or quadratic effects of the exposure time. The non‐fibrous carbohydrate (NFC) (minimum of 278 g/kg), in vitro dry‐matter digestibility (IVDMD) (minimum of 570 g/kg) and lactic acid (1.51 g/kg for each hour) decreased with longer exposure. The pH (0.011 for each hour), ammonia nitrogen (0.057 g/kg for each hour), acetic acid (maximum of 17.9 g/kg) and aerobic stability (maximum of 62.4 hr) increased with longer exposure. Although silage exposure reduced NFC and IVDMD and increased pH and ammonia nitrogen, animal feed efficiency was not compromised. These results indicate that sorghum silage exposure for up to 24 hr does not compromise its use.
    Type of Medium: Online Resource
    ISSN: 0142-5242 , 1365-2494
    URL: Issue
    URL: Article
    DOI: 10.1111/gfs.v76.3
    DOI: 10.1111/gfs.12538
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2016528-6
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