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  • 1
    Online Resource
    Online Resource
    Deportment of cobalt bismuth nanoferrites in Kevlar‐supported c ellulose acetate membranes for heavy metal‐salts rejection profile
    Wiley ; 2022
    In:  Journal of Applied Polymer Science Vol. 139, No. 40 ( 2022-10-20)
    Details
    In: Journal of Applied Polymer Science, Wiley, Vol. 139, No. 40 ( 2022-10-20)
    Abstract: The designed novel hybrid laminate membranes have been synthesized with silane surface functionalized Kevlar fabric packed between two layers of cobalt bismuth nanoferrites (CBF)‐loaded cellulose acetate. Primarily, nanoreinforcement; Co‐Bi nano‐ferrites were synthesized using sol–gel technique and functionalization is carried out using 3‐aminpropyl‐tri‐methoxysilane. Secondarily, thermally assisted evaporation methodology coupled with dip‐coating is adopted to synthesize hybrid Kevlar laminate membranes. Finally, structural, thermal, and flux along rejection studies are done on resultant hybrid membranes. The surface morphology, porosity generation, and homogeneous distribution of nanoincorporation with various concentration are revealed from micrographs of SEM of membranes. Noteworthy, impact of nanoreinforcement has been observed to holdup thermal oxidation of synthesized unique formulations of hybrid membranes employing TGA. Presence of CBF in cellulose acetate (CA)/Kevlar laminate membranes are modified glass transition and crystallization temperatures as cleared in DSC. De‐ionized water for flux and heavy metal salts rejection capabilities of each formulated membrane were tested at applied pressures 60, 120, and 180 psi. The important findings are indicated that, increasing concentration of CBF loading in CA remarkably enhanced efficacy of metal‐nitrates rejection with membranes. It is attributed to sole magneto‐physiochemical behavior of nanoferrites that, facilitate enhanced adsorption in designed hybrid membranes for heavy metal rejection from industrial wastes.
    Type of Medium: Online Resource
    ISSN: 0021-8995 , 1097-4628
    URL: Issue
    URL: Article
    DOI: 10.1002/app.v139.40
    DOI: 10.1002/app.52962
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 1491105-X
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  • 2
    Online Resource
    Online Resource
    Investigation of 500 keV Si ion induced surface structuring of Ni and Ti for enhancement of field emission properties
    Wiley ; 2023
    In:  Surface and Interface Analysis Vol. 55, No. 2 ( 2023-02), p. 113-126
    Details
    In: Surface and Interface Analysis, Wiley, Vol. 55, No. 2 ( 2023-02), p. 113-126
    Abstract: The present paper reports the investigation of surface morphology, elemental composition, phase changes and field emission properties of Si ion irradiated nickel (Ni) and titanium (Ti). The Ni and Ti targets have been irradiated with 500 keV Si ions generated by Pelletron accelerator at various fluences ranging from 6.9 × 10 13 to 77.1 × 10 13 ions/cm 2 . Stopping range of ions in matter analysis revealed higher values of electronic stopping and sputtering yield for Ni as compared with Ti. For both irradiated metals, electronic energy loss dominant over the nuclear stopping. The growth of induced surface structures have been analysed by using field emission scanning electron microscopy (FESEM) analysis. In case of Ni, as the ion fluence increases from 6.9 × 10 13 to 65.8 × 10 13 ions/cm 2 , the formation of spherical particulates, agglomers and sputtering is observed. Although in the case of Ti, with the increase of Si ion fluence from 11.6 × 10 13 to 77.1 × 10 13 ions/cm 2 , the formation of irregular‐shaped particulates along with crater and sputtered channels is observed. X‐ray diffraction (XRD) analysis shows that no new phase is identified. However, a significant increase in peak intensity is observed with increasing ion fluence. The variation in crystallite size and dislocation line density is also observed as a function of Si ion fluence. Fourier transform infrared spectroscopy analysis shows that no bands are formed after the Si ion irradiation. Field emission properties of ion‐structured Ni and Ti are well correlated with the growth of surface structures observed by SEM and dislocation line density evaluated by XRD analysis.
    Type of Medium: Online Resource
    ISSN: 0142-2421 , 1096-9918
    URL: Issue
    URL: Article
    DOI: 10.1002/sia.v55.2
    DOI: 10.1002/sia.7169
    RVK:
    ZG 1100
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2023881-2
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  • 3
    Online Resource
    Online Resource
    Molecular characterization of recombinant premembrane protein of dengue virus serotype‐2 for development of diagnostic assay
    Wiley ; 2023
    In:  Journal of Basic Microbiology Vol. 63, No. 5 ( 2023-05), p. 489-498
    Details
    In: Journal of Basic Microbiology, Wiley, Vol. 63, No. 5 ( 2023-05), p. 489-498
    Abstract: Dengue is an acute arboviral infection common in tropical and subtropical countries. Dengue has been highlighted as a public health concern in the last five decades, affecting almost 50% of the population in developing nations. Dengue infection results in a complex symptomatic disease that ranges from headache, fever, and skin rash to extreme hemorrhage fever and liver dysfunction. The diagnosis of the disease is essential for effective treatment. The early onset of the infection can be detected through viral structural peptides that act as markers for detection, including Pre‐Membrane (Pre‐M) protein. In the currently proposed research, the structural gene obtained from local isolates was targeted for studies. For this purpose, recombinant structural protein Pre‐M was amplified, cloned, and expressed in the bacterial expression system. The expression of the structural protein (Pre‐M) was scrutinized by Sodium Dodecyl Sulphate‐Polyacrylamide Gel Electrophoresis (SDS‐PAGE) and validated by western blot and dot blot, and afterwards, the antigen was purified. The purified Pre‐M protein carries the potential for the development of in‐house diagnostic assay as well as for vaccine production. This study aimed to develop a highly specific, sensitive, and cost‐effective in‐house enzyme‐linked immunoassay (ELISA) for the detection of antibodies of Pakistani most prevalent dengue virus serotype 2 (DENV‐2). The success of this research would also pave the way toward developing novel vaccines for the future prevention of dengue infection.
    Type of Medium: Online Resource
    ISSN: 0233-111X , 1521-4028
    URL: Issue
    URL: Article
    DOI: 10.1002/jobm.v63.5
    DOI: 10.1002/jobm.202200469
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1480967-9
    detail.hit.zdb_id: 632513-0
    detail.hit.zdb_id: 203025-1
    SSG: 12
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  • 4
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    Online Resource
    Fabrication of alumina based electrically conductive polymer composites
    Wiley ; 2016
    In:  Journal of Applied Polymer Science Vol. 133, No. 5 ( 2016-02-05)
    Details
    In: Journal of Applied Polymer Science, Wiley, Vol. 133, No. 5 ( 2016-02-05)
    Abstract: Novel electrically conductive composites were synthesized by incorporating Cu coated alumina (Cu‐Al 2 O 3 ) powder prepared via electroless plating technique as filler (0–21wt %) into polystyrene‐ b ‐methylmethacrylate (PS‐ b ‐PMMA) and polystyrene (PS) matrices. XRD analysis depicted maximum Cu crystallite growth (26.116 nm∼ plating time 30 min) onto Al 2 O 3 along with a significant change in XRD patterns of composites with Cu‐Al 2 O 3 inclusion. SEM–EDX analyses exhibited uniform Cu growth onto Al 2 O 3 and confirmed presence of Cu, Al, Pd in Cu‐Al 2 O 3 , and C, O, Al, Cu, and Pd in PS‐ b ‐PMMA and PS composites. Increasing filler loadings exhibited increased electrical conductivity (5.55 × 10 −5 S/cm for PS‐ b ‐PMMA; 5.0 × 10 −6 S/cm for PS) with increased Young's modulus (1122MPa for PS‐ b ‐PMMA; 1053.9MPa for PS) and tensile strength (27.998MPa for PS‐ b ‐PMMA; 30.585MPa for PS) and decreased % elongation. TGA demonstrated increased thermal stability and DTG revealed two‐step degradation in composites while DSC depicted pronounced increment in T g of Cu‐Al 2 O 3 /PS‐ b ‐PMMA with increased filler loading. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133 , 42939.
    Type of Medium: Online Resource
    ISSN: 0021-8995 , 1097-4628
    URL: Issue
    URL: Article
    DOI: 10.1002/app.v133.5
    DOI: 10.1002/app.42939
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 1491105-X
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  • 5
    Online Resource
    Online Resource
    Genome‐wide methylation profiling of HCV pathogenesis to develop diabetes and diabetic complications
    Wiley ; 2021
    In:  Journal of Viral Hepatitis Vol. 28, No. 2 ( 2021-02), p. 245-259
    Details
    In: Journal of Viral Hepatitis, Wiley, Vol. 28, No. 2 ( 2021-02), p. 245-259
    Abstract: HCV is key pathological factor for inducting insulin resistance. Such HCV‐induced insulin resistance is linked with metabolic syndrome, type 2 diabetes mellitus, extrahepatic manifestations, hepatic fibrosis progression and development of hepatocellular carcinoma. DNA methylation alterations can cause developmental abnormalities, tumours and other diseases. In our study, PBMCs were isolated and genomic DNA was extracted. DNA fragmentation was achieved by sonication to 200‐400 bp; subsequently, end repair and adenylation was performed. Manufacturer's guidelines were followed to ligate Cytosine‐methylated barcodes to sonicated DNA. EZ DNA Methylation‐GoldTM Kit was then employed to treat these DNA segments twice with bisulphite. A Library was maintained, sequenced on an Illumina platform and 150/125 bp paired‐end reads generated. GO seq R package was used to perform Gene Ontology (GO) enrichment analysis for genes linked to DMRs and DMPs; gene length bias was corrected. We identified 12 945 significant hypermethylated DMRs among all samples that were screened as those with at least 0.1 methylation level differences and P ‐value less than 0.05. Fisher's exact test with FDR multiple test correction is used for identification of DMPs and DMRs. High throughput bisulphite sequencing (Illumina) was carried out, and bioinformatics analysis was performed to analyse methylation status. Gene ontology (GO) and KEGG pathway enrichment analysis showed differentially methylated regions enriched in various pathways that include PI3K‐AKT/IRS1 signalling pathway, metabolic pathway, oxidative phosphorylation, Renin‐angiotensin system that are all involved in developing type‐2 diabetes (T2D). Our study provides supporting evidence for significant involvement of HCV infection in development of epigenetic modifications in regulation of metabolic disorders like T2D and its complications.
    Type of Medium: Online Resource
    ISSN: 1352-0504 , 1365-2893
    URL: Issue
    URL: Article
    DOI: 10.1111/jvh.v28.2
    DOI: 10.1111/jvh.13417
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2007924-2
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  • 6
    Online Resource
    Online Resource
    Co‐occurrence of aflatoxins and ochratoxin A in nuts, dry fruits, and nuty products
    Wiley ; 2018
    In:  Journal of Food Safety Vol. 38, No. 4 ( 2018-08)
    Details
    In: Journal of Food Safety, Wiley, Vol. 38, No. 4 ( 2018-08)
    Abstract: A total of 320 samples of edible nuts (poppy seed, peanut, pistachio, cashew, almonds) and dry fruits (plum, raisins, figs, dates, apricot, watermelon seed, melon seeds, pomegranate seeds) collected from major cities of Punjab, and Khyber Pakhtunkhwa, Pakistan, were analyzed for the presence of aflatoxins (AFs) and ochratoxin A (OTA). Sample extracts were purified using immunoaffinity columns and analyzed by reversed phase HPLC with fluorescence detection. Aflatoxin B 1 (AFB 1 ) and total AFs (AFB 1 , AFB 2 , AFG 1 , AFG 2 ) were found in 128 (40%) samples, and total AFs exceeded levels of 4 µg/kg and 10 µg/kg in 34 and 25% of samples. The highest mean levels of total AFs (7.30 ± 1.80 µg/kg) were found in peanuts without shell, the lowest mean levels (2.90 ± 1.50 µg/kg) were found in watermelon seeds with shell samples. OTA was found in 18 (5%) samples, the highest levels (5.60 ± 1.34 µg/kg) were found in raisins (maximum 18.5 µg/kg). Only four samples of edible nuts and dry fruits were contaminated with OTA at levels above the recommended limit of 10 µg/kg. The results have revealed the co‐occurrence of OTA and total AFs in 25% total samples of dry fruits and nuts with a mean level of OTA 3.58 ± 1.30 µg/kg and total AFs mean level of 4.13 ± 0.48 µg/kg, respectively. The high frequency especially of AFs in dry fruits and edible nuts is regarded as a health issue for consumers. Practical applications The manuscript has provided the current status on the levels of AFs and OTA in dry fruits and nuts in Pakistan. The results of current finding revealed that the levels of AFs and OTA in those samples which have stored in shells are significantly less as compared to those samples of nuts and dry fruits which have been stored without shells. Therefore, to use good storage practices, farmers, venders, and traders can eliminate or minimize the levels of these toxins in their products. Furthermore, the study will provide the awareness and levels of AFs and OTA in nuts and dry fruits and help to implement strict regulations for these toxins by law enforcement agencies.
    Type of Medium: Online Resource
    ISSN: 0149-6085 , 1745-4565
    URL: Issue
    URL: Article
    DOI: 10.1111/jfs.2018.38.issue-4
    DOI: 10.1111/jfs.12462
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2179788-2
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  • 7
    Online Resource
    Online Resource
    Manganese spinel ferrites‐composite nanotubes impregnated thermally endured cellulose acetate membranes for superior desalination application
    Wiley ; 2023
    In:  Asia-Pacific Journal of Chemical Engineering Vol. 18, No. 4 ( 2023-07)
    Details
    In: Asia-Pacific Journal of Chemical Engineering, Wiley, Vol. 18, No. 4 ( 2023-07)
    Abstract: Manganese nanoferrites are decorated over single‐wall carbon nanotubes using in situ co‐precipitation technique to incorporate into cellulose acetate (CA) polymer in order to prepare nanocomposite membranes for salt rejection and desalination purpose. The microstructural study reveals the pore size of the synthesized membranes from 5 nm to 0.20 μm depending upon the loading concentration of the impregnated nanocomposite tubes. Fourier transform infrared (FTIR) and X‐ray diffraction (XRD) patterns of the synthesized membranes delineate the presence of decorated nanotubes in the host polymer matrix. Thermal degradation study proclaims the thermal endurance enhancement with increasing nanotubes concentration in the polymer matrix. Flux rate of the deionized (DI) water through the prepared membranes decreases with increasing nano‐filler concentration while this impact is observed inverse in salt rejection study of the aluminum sulphate (Al 2 (SO 4 ) 3 ) and zinc sulphate (ZnSO 4 ) solutions due to the transformation of microporosity to nanoporosity with increasing composite tubes dosing into the host CA polymer.
    Type of Medium: Online Resource
    ISSN: 1932-2135 , 1932-2143
    URL: Issue
    URL: Article
    DOI: 10.1002/apj.v18.4
    DOI: 10.1002/apj.2914
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2276947-X
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  • 8
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    Online Resource
    Impact of Seawater on Distribution of Fluoride and Other Ions in Groundwater of Diplo Area, Thar Desert Pakistan
    Wiley ; 2013
    In:  Water Environment Research Vol. 85, No. 7 ( 2013-07), p. 579-586
    Details
    In: Water Environment Research, Wiley, Vol. 85, No. 7 ( 2013-07), p. 579-586
    Abstract: The role of seawater dilution, along with other water‐quality components, has been studied to determine the causes of spatial distribution of high fluoride concentrations in the groundwater of the Diplo sub‐district, Thar Desert, Pakistan. Fluoride ion concentration ranges of up to 7.60 mg/L were discovered, with mean and median values of 1.66 and 1.34 mg/L, respectively. Estimates based on the total dissolved solid (TDS) ratio show the impact of seawater intrusion at a percentage of around 8.05% in the groundwater. The major ion chemistry of water in the central diamond shape of the Piper diagram precisely demarcates the phenomenon of the intrusion of seawater into the study area. The plots of Na+K vs. Ca+Mg (meq/L) and log Cl (mg/L) vs. log Ca+Mg/Na+K (meq/L) indicate a mixing of freshwater with seawater. The molar Na/Na+Cl and Ca/Ca+SO 4 ratios of the groundwater, and correlation matrices of major ion chemistry, also show a blending of groundwater with seawater. Mutual relationships among Li + , Sr 2+ , and Cl − ions further substantiate the marked influence of the marine environment on the groundwater in the study area.
    Type of Medium: Online Resource
    ISSN: 1061-4303 , 1554-7531
    URL: Article
    DOI: 10.2175/106143013X13698672321382
    RVK:
    AR 10100
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 1098976-6
    detail.hit.zdb_id: 2051010-X
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