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  • 1
    Online Resource
    Online Resource
    Short‐term toxicity assessment of combined use of zidovudine, lamivudine and lopinavir/ritonavir in vitro and in vivo
    Wiley ; 2023
    In:  Basic & Clinical Pharmacology & Toxicology Vol. 133, No. 1 ( 2023-07), p. 82-97
    Details
    In: Basic & Clinical Pharmacology & Toxicology, Wiley, Vol. 133, No. 1 ( 2023-07), p. 82-97
    Abstract: A combination of zidovudine (AZT), lamivudine (3TC) and lopinavir/ritonavir (LPV/r) is one of the most effective drugs for preventing mother‐to‐child transmission (PMTCT) of HIV. However, limited information is available regarding its systemic toxicity. This study aimed to investigate its potential toxicity. Method An acute oral toxicity test was conducted to assess the potential acute toxicity of AZT + 3TC + LPV/r. Bacterial reverse mutation, mammalian erythrocyte micronucleus and mouse spermatogonia chromosomal aberration tests were conducted to assess its potential genotoxicity. A 28‐day feeding test was conducted to assess the potential subacute toxicity. Results In mice, the LD 50 of the AZT + 3TC + LPV/r mixture was greater than 2000 mg/kg body weight (BW). The rate of micronucleated polychromatic erythrocytes (PCEs) increased in a dose‐dependent manner in mice ( P   〈  0.01). After treatment with AZT + 3TC + LPV/r for 28 days, the BW gain of male and female rats in the high‐dose group was lower than that in the control group ( P   〈  0.05); the relative weights of the liver, kidney, spleen and brain increased ( P   〈  0.05); and pathological abnormalities appeared in the thyroid and spleen of male and female rats in the high‐dose group. The haemoglobin (HGB) and red blood cells (RBCs) count in male and female rats decreased, but the white blood cells (WBCs) and lymphocyte apoptosis rates in male and female rats in the high‐dose group increased ( P   〈  0.05). The total protein, albumin, cholesterol and blood glucose levels of male and female rats in the high‐dose group were significantly decreased ( P   〈  0.05). The alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatinine (Cr) and blood urea nitrogen (BUN) levels of male and female rats in the medium‐ and high‐dose groups increased significantly ( P   〈  0.05). Conclusion The results suggest that AZT + 3TC + LPV/r may exhibit genotoxicity and subacute toxicity under experimental conditions.
    Type of Medium: Online Resource
    ISSN: 1742-7835 , 1742-7843
    URL: Issue
    URL: Article
    DOI: 10.1111/bcpt.v133.1
    DOI: 10.1111/bcpt.13870
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2151592-X
    SSG: 15,3
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  • 2
    Online Resource
    Online Resource
    Tailoring the Microporosity of Polymers of Intrinsic Microporosity for Advanced Gas Separation by Atomic Layer Deposition
    Wiley ; 2021
    In:  Angewandte Chemie International Edition Vol. 60, No. 33 ( 2021-08-09), p. 17875-17880
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 60, No. 33 ( 2021-08-09), p. 17875-17880
    Abstract: Tailoring the microporosity of intrinsically microporous polymers at the atomic level is one of the biggest challenges in achieving high‐performance polymeric gas separation membranes. In this study, for the first time, the Al 2 O 3 atomic layer deposition (ALD) technique was used to modify the microporosity of a typical polymer of intrinsic microporosity (PIM‐1) at the atomic level. PIM‐1 with six ALD cycles (PIM‐1‐Al 2 O 3 ‐6) exhibited simultaneous high thermal, mechanical, pure‐ and mixed‐gas separation, and anti‐aging properties. The O 2 /N 2 , H 2 /N 2 , and H 2 /CH 4 separation performances were adequate above the latest trade‐off lines. PIM‐1‐Al 2 O 3 ‐6 showed CO 2 and O 2 permeabilities of 624 and 188 Barrer, combined with CO 2 /CH 4 and O 2 /N 2 selectivities of 56.2 and 8.8, respectively. This significantly enhanced performance was attributed to the strong size sieving effect induced by the Al 2 O 3 deposition.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v60.33
    DOI: 10.1002/anie.202016901
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
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  • 3
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    Online Resource
    Single‐cell RNA analysis on ACE2 expression provides insights into SARS‐CoV‐2 potential entry into the bloodstream and heart injury
    Wiley ; 2020
    In:  Journal of Cellular Physiology Vol. 235, No. 12 ( 2020-12), p. 9884-9894
    Details
    In: Journal of Cellular Physiology, Wiley, Vol. 235, No. 12 ( 2020-12), p. 9884-9894
    Abstract: Coronavirus disease‐2019 (COVID‐19) is a global pandemic with high infectivity and pathogenicity, accounting for tens of thousands of deaths worldwide. Recent studies have found that the pathogen of COVID‐19, severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), shares the same cell receptor angiotensin converting enzyme II (ACE2) as SARS‐CoV. The pathological investigation of COVID‐19 deaths showed that the lungs had characteristics of pulmonary fibrosis. However, how SARS‐CoV‐2 spreads from the lungs to other organs has not yet been determined. Here, we performed an unbiased evaluation of cell‐type‐specific expression of ACE2 in healthy and fibrotic lungs, as well as in normal and failed adult human hearts, using published single‐cell RNA‐seq data. We found that ACE2 expression in fibrotic lungs mainly locates in arterial vascular cells, which might provide a route for bloodstream spreading of SARS‐CoV‐2. Failed human hearts have a higher percentage of ACE2‐expressing cardiomyocytes, and SARS‐CoV‐2 might attack cardiomyocytes through the bloodstream in patients with heart failure. Moreover, ACE2 was highly expressed in cells infected by respiratory syncytial virus or Middle East respiratory syndrome coronavirus and in mice treated by lipopolysaccharide. Our findings indicate that patients with pulmonary fibrosis, heart failure, and virus infection have a higher risk and are more susceptible to SARS‐CoV‐2 infection. The SARS‐CoV‐2 might attack other organs by getting into the bloodstream. This study provides new insights into SARS‐CoV‐2 blood entry and heart injury and might propose a therapeutic strategy to prevent patients from developing severe complications.
    Type of Medium: Online Resource
    ISSN: 0021-9541 , 1097-4652
    URL: Issue
    URL: Article
    DOI: 10.1002/jcp.v235.12
    DOI: 10.1002/jcp.29802
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 1478143-8
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Simple, fast and high‐throughput single‐cell analysis on PDMS microfluidic chips
    Wiley ; 2008
    In:  ELECTROPHORESIS Vol. 29, No. 24 ( 2008-12), p. 5055-5060
    Details
    In: ELECTROPHORESIS, Wiley, Vol. 29, No. 24 ( 2008-12), p. 5055-5060
    Abstract: This paper demonstrated the chemical analysis of single cell on a cross PDMS microfluidic chip in a simple, fast, and high‐throughput mode. The pre‐stained cells were sequentially loaded into the cross section by hydrodynamic force, lysed by 0.2% SDS and subsequently the lysates were detected by LIF. Each cell can be lysed within 500 ms due to its high concentration of SDS at cross section resulted from the absence of electroosmosis after surface coating in microchannel. The reliability and quality of the analysis was confirmed by analysis of glutathione and rhodamine 123 in single K562 cells. In each run, approximately 100 cells could be analyzed in about 10 min, which demonstrated the comparatively high throughput. The proposed microfluidic method is simple, fast, and high throughput, which might be of significance in identifying the biological molecules involved in fast biochemical processes and studying heterogenous cells.
    Type of Medium: Online Resource
    ISSN: 0173-0835 , 1522-2683
    URL: Issue
    URL: Article
    DOI: 10.1002/elps.v29:24
    DOI: 10.1002/elps.200800331
    Language: English
    Publisher: Wiley
    Publication Date: 2008
    detail.hit.zdb_id: 1475486-1
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Gram-Scale Aqueous Synthesis of Stable Few-Layered 1T-MoS 2 : Applications for Visible-Light-Driven Photocatalytic Hydrogen Evolution
    Wiley ; 2015
    In:  Small Vol. 11, No. 41 ( 2015-11), p. 5556-5564
    Details
    In: Small, Wiley, Vol. 11, No. 41 ( 2015-11), p. 5556-5564
    Type of Medium: Online Resource
    ISSN: 1613-6810
    URL: Issue
    URL: Article
    DOI: 10.1002/smll.v11.41
    DOI: 10.1002/smll.201501822
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2168935-0
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  • 6
    Online Resource
    Online Resource
    Tailoring the Microporosity of Polymers of Intrinsic Microporosity for Advanced Gas Separation by Atomic Layer Deposition
    Wiley ; 2021
    In:  Angewandte Chemie Vol. 133, No. 33 ( 2021-08-09), p. 18019-18024
    Details
    In: Angewandte Chemie, Wiley, Vol. 133, No. 33 ( 2021-08-09), p. 18019-18024
    Abstract: Tailoring the microporosity of intrinsically microporous polymers at the atomic level is one of the biggest challenges in achieving high‐performance polymeric gas separation membranes. In this study, for the first time, the Al 2 O 3 atomic layer deposition (ALD) technique was used to modify the microporosity of a typical polymer of intrinsic microporosity (PIM‐1) at the atomic level. PIM‐1 with six ALD cycles (PIM‐1‐Al 2 O 3 ‐6) exhibited simultaneous high thermal, mechanical, pure‐ and mixed‐gas separation, and anti‐aging properties. The O 2 /N 2 , H 2 /N 2 , and H 2 /CH 4 separation performances were adequate above the latest trade‐off lines. PIM‐1‐Al 2 O 3 ‐6 showed CO 2 and O 2 permeabilities of 624 and 188 Barrer, combined with CO 2 /CH 4 and O 2 /N 2 selectivities of 56.2 and 8.8, respectively. This significantly enhanced performance was attributed to the strong size sieving effect induced by the Al 2 O 3 deposition.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v133.33
    DOI: 10.1002/ange.202016901
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
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  • 7
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    Online Resource
    Prognosis prediction and risk factors for triple‐negative breast cancer patients with brain metastasis: A population‐based study
    Wiley ; 2023
    In:  Cancer Medicine Vol. 12, No. 7 ( 2023-04), p. 7951-7961
    Details
    In: Cancer Medicine, Wiley, Vol. 12, No. 7 ( 2023-04), p. 7951-7961
    Abstract: Brain metastasis (BM) in triple‐negative breast cancer (TNBC) patients is associated with significant morbidity and mortality. In this research we aimed to develop a nomogram to predict the prognosis of TNBC patients with BMs (TNBC‐BM) and explore the potential risk factors. Methods We used data from the Surveillance, Epidemiology, and End Results (SEER) database. A prognostic nomogram was built and validated based on patients with BM at newly diagnosed TNBC (nTNBC‐BM). Its effect on TNBC patients with BM was also validated in an extended group. The prognostic effect of treatment and risk factors for nTNBC‐BM were further tested. Results A nomogram was constructed and validated to predict overall survival (OS) in TNBC‐BM patients. For patients with BM diagnosed at the initial treatment or later course, the C‐index (0.707, 0.801, and 0.685 in the training, validation, and extended groups, respectively) and calibration plots showed the acceptable prognostic accuracy and clinical applicability of the model. Surgery on the primary tumor and chemotherapy were found to confer significantly better OS (11 months vs. 4 months; 5 months vs. 3 months, respectively). In addition, advanced tumor/nodal stage and bilateral cancer were associated with a higher risk of nTNBC‐BM. Conclusion We developed a sensitive and discriminative nomogram to predict OS in TNBC‐BM patients, both at initial diagnosis and the latter course. nTNBC‐BM patients may benefit more from surgery and chemotherapy than from radiotherapy. In addition, in the predictive model, TNBC patients harboring advanced tumor/nodal stages and bilateral tumors were more likely to have BM at initial diagnosis.
    Type of Medium: Online Resource
    ISSN: 2045-7634 , 2045-7634
    URL: Issue
    URL: Article
    DOI: 10.1002/cam4.v12.7
    DOI: 10.1002/cam4.5575
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2659751-2
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  • 8
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    Online Resource
    The crystal structure of the TCP domain of PCF6 in Oryza sativa L. reveals an RHH‐like fold
    Wiley ; 2020
    In:  FEBS Letters Vol. 594, No. 8 ( 2020-04), p. 1296-1306
    Details
    In: FEBS Letters, Wiley, Vol. 594, No. 8 ( 2020-04), p. 1296-1306
    Abstract: The Teosinte branched 1/Cycloidea/Proliferating cell factor (TCP) domain is an evolutionarily conserved DNA binding domain unique to the plant kingdom. To date, the functions of TCPs have been well studied, but the three‐dimensional structure of the TCP domain is lacking. Here, we have determined the crystal structure of the TCP domain from OsPCF6. The structure reveals that the TCP domain adopts three short β‐strands followed by a helix–loop–helix structure, distinct from the canonical basic helix–loop–helix structure. This folded domain shows high structural similarity to the ribbon–helix–helix (RHH) transcriptional repressors, a family of DNA binding proteins with a conserved 3D structural motif (RHH fold), indicating that TCPs could be reclassified as RHH proteins. Our work will provide insight toward a better understanding of the mechanisms underlying TCP protein function.
    Type of Medium: Online Resource
    ISSN: 0014-5793 , 1873-3468
    URL: Issue
    URL: Article
    DOI: 10.1002/feb2.v594.8
    DOI: 10.1002/1873-3468.13727
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 1460391-3
    SSG: 12
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  • 9
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    Online Resource
    Structural basis for the Target DNA recognition and binding by the MYB domain of phosphate starvation response 1
    Wiley ; 2019
    In:  The FEBS Journal Vol. 286, No. 14 ( 2019-07), p. 2809-2821
    Details
    In: The FEBS Journal, Wiley, Vol. 286, No. 14 ( 2019-07), p. 2809-2821
    Abstract: The phosphate starvation response 1 ( PHR 1) protein has a central role in mediating the response to phosphate starvation in plants. PHR 1 is composed of a number of domains including a MYB domain involved with DNA binding and a coiled‐coil domain proposed to be involved with dimer formation. PHR 1 binds to the promoter of phosphate starvation‐induced genes to control the levels of phosphate required for nutrition. Previous studies have shown that both the MYB domain and the coiled‐coil domain of PHR 1 are required for binding the target DNA . Here, we describe the crystal structure of the PHR 1 MYB domain and two structures of its complex with the PHR 1‐binding DNA sequence (P1 BS ). Structural and isothermal titration calorimetry has been carried out showing that the MYB domain of PHR 1 alone is sufficient for target DNA recognition and binding. Two copies of the PHR 1 MYB domain bind to the same major groove of the P1 BS DNA with few direct interactions between the individual MYB domains. In addition, the PHR 1 MYB –P1 BS DNA complex structures reveal amino acid residues involved in DNA recognition and binding. Mutagenesis of these residues results in lost or impaired ability of PHR 1 MYB to bind to its target DNA . The results presented reveal the structural basis for DNA recognition by the PHR 1 MYB domain and demonstrate that two PHR 1 MYB domains attach to their P1 BS DNA targeting sequence. Database Coordinates and structure factors have been deposited in the Protein Data Bank under accession codes 6J4K (PHR1 MYB), 6J4R (PHR1 MYB‐R‐P1BS), 6J5B (MYB‐CC‐R2‐P1BS).
    Type of Medium: Online Resource
    ISSN: 1742-464X , 1742-4658
    URL: Issue
    URL: Article
    DOI: 10.1111/febs.2019.286.issue-14
    DOI: 10.1111/febs.14846
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2172518-4
    SSG: 12
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  • 10
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    The crystal structure of Arabidopsis BON1 provides insights into the copine protein family
    Wiley ; 2020
    In:  The Plant Journal Vol. 103, No. 3 ( 2020-08), p. 1215-1232
    Details
    In: The Plant Journal, Wiley, Vol. 103, No. 3 ( 2020-08), p. 1215-1232
    Abstract: The Arabidopsis thaliana BON1 gene product is a member of the evolutionary conserved eukaryotic calcium‐dependent membrane‐binding protein family. The copine protein is composed of two C2 domains (C2A and C2B) followed by a vWA domain. The BON1 protein is localized on the plasma membrane, and is known to suppress the expression of immune receptor genes and to positively regulate stomatal closure. The first structure of this protein family has been determined to 2.5‐Å resolution and shows the structural features of the three conserved domains C2A, C2B and vWA. The structure reveals the third Ca 2+ ‐binding region in C2A domain is longer than classical C2 domains and a novel Ca 2+ binding site in the vWA domain. The structure of BON1 bound to Mn 2+ is also presented. The binding of the C2 domains to phospholipid (PSF) has been modeled and provides an insight into the lipid‐binding mechanism of the copine proteins. Furthermore, the selectivity of the separate C2A and C2B domains and intact BON1 to bind to different phospholipids has been investigated, and we demonstrated that BON1 could mediate aggregation of liposomes in response to Ca 2+ . These studies have formed the basis of further investigations into the important role that the copine proteins play in vivo .
    Type of Medium: Online Resource
    ISSN: 0960-7412 , 1365-313X
    URL: Issue
    URL: Article
    DOI: 10.1111/tpj.v103.3
    DOI: 10.1111/tpj.14797
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2020961-7
    SSG: 12
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