In:
Biotechnology Journal, Wiley, Vol. 14, No. 8 ( 2019-08)
Abstract:
The fungal endophyte Cyanodermella asteris ( C. asteris ) has been recently isolated from the medicinal plant Aster tataricus ( A. tataricus ). This fungus produces astin C, a cyclic pentapeptide with anticancer and anti‐inflammatory properties. The production of this secondary metabolite is compared in immobilized and planktonic conditions. For immobilized cultures, a stainless steel packing immersed in the culture broth is used as a support. In these conditions, the fungus exclusively grows on the packing, which provides a considerable advantage for astin C recovery and purification. C. asteris metabolism is different according to the culture conditions in terms of substrate consumption rate, cell growth, and astin C production. Immobilized‐cell cultures yield a 30% increase of astin C production, associated with a 39% increase in biomass. The inoculum type as spores rather than hyphae, and a pre‐inoculation washing procedure with sodium hydroxide, turns out to be beneficial both for astin C production and fungus development onto the support. Finally, the influence of culture parameters such as pH and medium composition on astin C production is evaluated. With optimized culture conditions, astin C yield is further improved reaching a five times higher final specific yield compared to the value reported with astin C extraction from A. tataricus (0.89 mg g −1 and 0.16 mg g −1 respectively).
Type of Medium:
Online Resource
ISSN:
1860-6768
,
1860-7314
DOI:
10.1002/biot.201800624
Language:
English
Publisher:
Wiley
Publication Date:
2019
detail.hit.zdb_id:
2214038-4
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