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  • 1
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    Online Resource
    Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
    Wiley ; 2019
    In:  European Journal of Immunology Vol. 49, No. 10 ( 2019-10), p. 1457-1973
    Details
    In: European Journal of Immunology, Wiley, Vol. 49, No. 10 ( 2019-10), p. 1457-1973
    Abstract: These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer‐reviewed by leading experts in the field, making this an essential research companion.
    Type of Medium: Online Resource
    ISSN: 0014-2980 , 1521-4141
    URL: Issue
    URL: Article
    DOI: 10.1002/eji.v49.10
    DOI: 10.1002/eji.201970107
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1491907-2
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  • 2
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    Relative impacts of gypsy moth outbreaks and insecticide treatments on forest resources and ecosystems: An experimental approach
    Wiley ; 2021
    In:  Ecological Solutions and Evidence Vol. 2, No. 1 ( 2021-01)
    Details
    In: Ecological Solutions and Evidence, Wiley, Vol. 2, No. 1 ( 2021-01)
    Abstract: 1. Massenvermehrungen des Schwammspinners verursachen schwere Entlaubungen in holarktischen Wäldern, sowohl in Nordamerika als invasive Art als auch in ihrem natürlichen Verbreitungsgebiet in Eurasien. Eine Entlaubung kann die Holzproduktion beeinträchtigen und sich auf ökologische Gemeinschaften und Prozesse auswirken. Um ökonomische Schäden zu mindern, werden in Massenvermehrungsgebieten regelmäßig Insektizide mittels Luftfahrzeugen ausgebracht. Diese Einsätze können teuer sein und sich schädlich auf Nichtzielorganismen und Interaktionsnetzwerke von Arten auswirken. Allerdings gibt es nur wenige replizierte Studien, die relative Einflüsse von Schwammspinnerausbrüchen und der damit verbundenen Insektizidbehandlung auf Waldwachstum und Tiergemeinschaften untersuchen. Auch im natürlichen Verbreitungsgebiet der Art stehen entsprechende Untersuchungen noch aus. 2. Wir geben hier einen Überblick, auf welchen Wegen die Massenvermehrung des Schwammspinners und dessen chemische Bekämpfung Waldökosysteme beeinflusst. Anschließend stellen wir einen experimentellen Versuchsaufbau vor, der Anfang 2019 in Süd‐ und Mitteldeutschland etabliert wurde und darauf abzielt, die ökologischen und ökonomischen Konsequenzen der Schwammspinnerausbrüche und der Insektizidbehandlung in Eichenwäldern zu untersuchen. Das vollfaktorielle Design der Studie umfasst 12 experimentelle Versuchsflächen aus Waldbeständen mit hohem und niedrigem Entlaubungsrisiko, die entweder mit Tebufenozid behandelt wurden oder unbehandelt blieben. Waldwachstum und –struktur, Baumsterblichkeit, Schwammspinnerdichte sowie die Gemeinschaftsstruktur von Schmetterlingen, Vögeln, Fledermäusen, Laufkäfern und Arthropoden der Baumkronen werden über mehrere Jahre aufgenommen. 3. Ein Jahr intensives Monitoring der Schwammspinnerpopulation und der Schäden in den ausgewählten Versuchsflächen zeigten einen substanziellen Unterschied der Populationsdichte zwischen Flächen mit hohem und niedrigem Entlaubungsrisiko und eine hohe Wirksamkeit von Tebufenozid beim Unterdrücken der Schwammspinnerpopulation in behandelten Flächen. Im ersten Untersuchungsjahr unterschieden sich Schwammspinnerdichte und Entlaubung in prognostizierten Ausbruchsflächen stark, was die Wichtigkeit von vielen Replikaten und Blöcken bestätigt, um räumliche Heterogenität auszugleichen. Das Experiment wird kontinuierlich während der kommenden Jahre durchgeführt, um kurzfristige als auch mittelfristige ökonomische und ökologische Daten zu generieren, die zur Verbesserung unseres Verständnisses und des Managements von Schwammspinnerausbrüchen beitragen werden.
    Type of Medium: Online Resource
    ISSN: 2688-8319 , 2688-8319
    URL: Issue
    URL: Article
    DOI: 10.1002/eso3.v2.1
    DOI: 10.1002/2688-8319.12045
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 3021448-8
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  • 3
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    Shared genetic risk between eating disorder‐ and substance‐use‐related phenotypes: Evidence from genome‐wide association studies
    Wiley ; 2021
    In:  Addiction Biology Vol. 26, No. 1 ( 2021-01)
    Details
    In: Addiction Biology, Wiley, Vol. 26, No. 1 ( 2021-01)
    Abstract: Eating disorders and substance use disorders frequently co‐occur. Twin studies reveal shared genetic variance between liabilities to eating disorders and substance use, with the strongest associations between symptoms of bulimia nervosa and problem alcohol use (genetic correlation [ r g ], twin‐based = 0.23‐0.53). We estimated the genetic correlation between eating disorder and substance use and disorder phenotypes using data from genome‐wide association studies (GWAS). Four eating disorder phenotypes (anorexia nervosa [AN] , AN with binge eating, AN without binge eating, and a bulimia nervosa factor score), and eight substance‐use‐related phenotypes (drinks per week, alcohol use disorder [AUD], smoking initiation, current smoking, cigarettes per day, nicotine dependence, cannabis initiation, and cannabis use disorder) from eight studies were included. Significant genetic correlations were adjusted for variants associated with major depressive disorder and schizophrenia. Total study sample sizes per phenotype ranged from ~2400 to ~537 000 individuals. We used linkage disequilibrium score regression to calculate single nucleotide polymorphism‐based genetic correlations between eating disorder‐ and substance‐use‐related phenotypes. Significant positive genetic associations emerged between AUD and AN ( r g = 0.18; false discovery rate q = 0.0006), cannabis initiation and AN ( r g = 0.23; q 〈 0.0001), and cannabis initiation and AN with binge eating ( r g = 0.27; q = 0.0016). Conversely, significant negative genetic correlations were observed between three nondiagnostic smoking phenotypes (smoking initiation, current smoking, and cigarettes per day) and AN without binge eating ( r gs = −0.19 to −0.23; qs 〈 0.04). The genetic correlation between AUD and AN was no longer significant after co‐varying for major depressive disorder loci. The patterns of association between eating disorder‐ and substance‐use‐related phenotypes highlights the potentially complex and substance‐specific relationships among these behaviors.
    Type of Medium: Online Resource
    ISSN: 1355-6215 , 1369-1600
    URL: Issue
    URL: Article
    DOI: 10.1111/adb.v26.1
    DOI: 10.1111/adb.12880
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 1495537-4
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  • 4
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    Guidelines for the use of flow cytometry and cell sorting in immunological studies *
    Wiley ; 2017
    In:  European Journal of Immunology Vol. 47, No. 10 ( 2017-10), p. 1584-1797
    Details
    In: European Journal of Immunology, Wiley, Vol. 47, No. 10 ( 2017-10), p. 1584-1797
    Type of Medium: Online Resource
    ISSN: 0014-2980 , 1521-4141
    URL: Issue
    URL: Article
    DOI: 10.1002/eji.v47.10
    DOI: 10.1002/eji.201646632
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 1491907-2
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  • 5
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    Stereoselective transport of hydrophilic quaternary drugs by human MDR1 and rat Mdr1b P‐glycoproteins
    Wiley ; 2002
    In:  British Journal of Pharmacology Vol. 135, No. 7 ( 2002-04), p. 1685-1694
    Details
    In: British Journal of Pharmacology, Wiley, Vol. 135, No. 7 ( 2002-04), p. 1685-1694
    Abstract: The present study was performed to evaluate and compare the ability of human MDR1 ‐, and rat Mdr1b ‐ and Mdr2 ‐P‐glycoproteins to transport hydrophilic monoquaternary drugs. Transport studies were performed with plasma membrane vesicles isolated from MDR1‐, Mdr1b‐, or Mdr2‐overexpressing insect cells. As model substrates we used the N ‐methylated derivatives of the diastereomers quinidine and quinine, the monoquaternary compounds N ‐methylquinidine and N ‐methylquinine. Vincristine, an established MDR1 substrate, was used as a reference. We observed ATP‐dependent uptake of all drugs studied into MDR1‐ and Mdr1b‐expressing vesicles. Mdr2 was not able to transport these compounds. MDR1‐ and Mdr1b‐mediated transport was saturable, and could be inhibited by various drugs, including PSC‐833. For both MDR1 and Mdr1b the V max / K m ratios (or clearance) of N ‐methylquinidine were greater than those determined for N ‐methylquinine. This stereoselective difference was also evident from differential inhibitory studies with the two isomers. Comparison of normalized clearance indicated that human MDR1 was more effective in transporting the tested substrates than rat Mdr1b. In conclusion, our results demonstrate that MDR1 and Mdr1b, but not Mdr2, are able to transport the monoquaternary model drugs; both MDR1 and Mdr1b display stereospecificity for these cations; and indicate human MDR1 is more efficient in transporting these cations than its rat orthologue Mdr1b. British Journal of Pharmacology (2002) 135 , 1685–1694; doi: 10.1038/sj.bjp.0704620
    Type of Medium: Online Resource
    ISSN: 0007-1188 , 1476-5381
    URL: Article
    DOI: 10.1038/sj.bjp.0704620
    Language: English
    Publisher: Wiley
    Publication Date: 2002
    detail.hit.zdb_id: 2029728-2
    SSG: 15,3
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  • 6
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    Statins and oral biofilm: Simvastatin as a promising drug to control periodontal dysbiosis
    Wiley ; 2022
    In:  Oral Diseases
    Details
    In: Oral Diseases, Wiley
    Abstract: This study evaluated antimicrobial activity of atorvastatin, pravastatin, rosuvastatin, and simvastatin against oral bacteria, and the interaction of simvastatin with standard antimicrobials (amoxicillin and metronidazole). Methods Minimal inhibitory concentration assays were performed with Porphyromonas gingivalis , Prevotella intermedia , Fusobacterium nucleatum , Actinomyces odontolyticus , Streptococcus oralis , Streptococcus mitis , Streptococcus salivarius , Streptococcus sanguinis , and Streptococcus gordonii ; checkerboard microdilution assays between simvastatin and standard antimicrobials; monospecies and multispecies biofilms. Results Simvastatin showed the best antimicrobial activity against most species (MIC range from 3.12 to 25 μg/ml), highlighting the sensitivity of P. gingivalis . In the checkerboard assay, synergistic interaction was found between simvastatin and amoxicillin against S. oralis and S. sanguinis . P. gingivalis biofilm was inhibited by simvastatin at 10 and 50× Minimal inhibitory concentration, with similar effects to metronidazole. For multispecies biofilm, SMV reduced the biofilm metabolic activity (79%) and total counts (87%), comparable to amoxicillin. Simvastatin also reduced bacterial counts of Veilonnella parvula , P. gingivalis , Streptococcus mutans , Actinomyces naeslundii , P. intermedia , and Capnocytophaga ochracea in the multispecies biofilm. Conclusions Simvastatin showed antimicrobial and antibiofilm activity against oral bacteria and may contribute to the control of dysbiosis, and may be considered in clinical studies as an adjuvant in the treatment of periodontitis.
    Type of Medium: Online Resource
    ISSN: 1354-523X , 1601-0825
    URL: Article
    DOI: 10.1111/odi.14446
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2008428-6
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  • 7
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    Direct Trace Element Determination in Oil and Gas Produced Waters with Inductively Coupled Plasma‐Optical Emission Spectrometry: Advantages of High‐Salinity Tolerance
    Wiley ; 2020
    In:  Geostandards and Geoanalytical Research Vol. 44, No. 2 ( 2020-06), p. 385-397
    Details
    In: Geostandards and Geoanalytical Research, Wiley, Vol. 44, No. 2 ( 2020-06), p. 385-397
    Abstract: Waters co‐produced during petroleum extraction are the largest waste streams from oil and gas development. Reuse or disposal of these waters is difficult due to their high salinities and the sheer volumes generated. Produced waters (PWs) may also contain valuable mineral commodities. While an understanding of produced water trace element composition is required for evaluating the associated resource and waste potential of these materials, measuring trace elements in brines is challenging due to the dilution requirements of typical methods. Alternatively, inductively coupled plasma‐optical emission spectrometry (ICP‐OES) has shown promise as being capable of direct measurements of trace elements within PWs with minimal dilution. Here, we evaluate direct ICP‐OES trace element quantification in PWs for seventeen trace elements (As, Al, Ba, Be, Cd, Cr, Co, Cu, Hg, Mo, Ni, Pb, Rb, Sb, U, V and Zn) within fifteen PWs from five U.S. continuous reservoirs. The total analytical uncertainties associated with the trace element levels determined using ICP‐OES were estimated to be better than ± 30% (2 s ) except for Rb, which could not be determined due to ionisation interferences. The ICP‐OES results are compared with trace element levels determined using inductively coupled plasma‐mass spectrometry from the same samples. Our results demonstrate the potential for direct analysis of high‐salinity waters using ICP‐OES with minimal dilution and provide trace element concentrations in waters from several important U.S. petroleum‐generating reservoirs where available data are sparse.
    Type of Medium: Online Resource
    ISSN: 1639-4488 , 1751-908X
    URL: Issue
    URL: Article
    DOI: 10.1111/ggr.v44.2
    DOI: 10.1111/ggr.12316
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2276474-4
    detail.hit.zdb_id: 2595219-5
    detail.hit.zdb_id: 2134777-3
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  • 8
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    Microbiota of the conjunctival sac of 120 healthy cats
    Wiley ; 2019
    In:  Veterinary Ophthalmology Vol. 22, No. 3 ( 2019-05), p. 328-336
    Details
    In: Veterinary Ophthalmology, Wiley, Vol. 22, No. 3 ( 2019-05), p. 328-336
    Abstract: To describe the bacterial and fungal microbiota of the conjunctiva and factors influencing these microbiota of healthy cats. To evaluate the antimicrobial resistance profile and discuss the use of appropriate antimicrobial drugs. Animals studied One hundred and twenty healthy cats Procedures Conjunctival samples with dry or premoistened swabs were obtained from both eyes and cultured for aerobic and anaerobic bacteria and fungi. In vitro bacterial, susceptibility testing was performed. The effects of age, sex, breed, season, living environment, and sample collection technique on the frequency and composition of microbial isolation were evaluated. Results In 49 of 120 cats (40.8%) and 73 of 240 swabs (30.4%), microorganisms were isolated. Of the isolates, 71% (61/86) were Gram‐positive bacteria, 26% (22/86) were Gram‐negative bacteria, and 3% (3/86) were fungi. Staphylococcus felis (17/86; 19.8%) was the most commonly isolated species. Moraxella osloensis (5/86; 5.8%) was the most frequent Gram‐negative species. The season had a statistically significant influence ( P  = 0.04) on the frequency of isolation. The use of premoistened swabs increased the rate of Gram‐positive bacterial detection significantly ( P  = 0.03). The in vitro susceptibility testing showed high efficacy of chloramphenicol, gentamicin, pradofloxacin, and enrofloxacin. Conclusions The isolated microbiota aligns with previous studies performed in other countries, although the microbiota of cats in the present study showed wider bacterial diversity, and more Gram‐negative species were isolated. Swab sampling should be performed with premoistened swabs. The topical antimicrobials gentamicin and chloramphenicol are suitable therapeutics for first‐line treatment.
    Type of Medium: Online Resource
    ISSN: 1463-5216 , 1463-5224
    URL: Issue
    URL: Article
    DOI: 10.1111/vop.2019.22.issue-3
    DOI: 10.1111/vop.12598
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2011043-1
    SSG: 22
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  • 9
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    A retrospective cohort study of clinical factors, visit patterns, and demographic factors associated with use of remote communications in patients with headache
    Wiley ; 2021
    In:  Headache: The Journal of Head and Face Pain Vol. 61, No. 10 ( 2021-11), p. 1521-1528
    Details
    In: Headache: The Journal of Head and Face Pain, Wiley, Vol. 61, No. 10 ( 2021-11), p. 1521-1528
    Abstract: To identify the characteristics associated with high utilization of remote communications (RCs) in patients with headache. Background Patients with headache frequently communicate with their providers using secure portal messaging and telephone calls. However, clinical and demographic factors as well as visit patterns associated with RC utilization remain poorly characterized. Methods We retrospectively analyzed data from patients with headache who were evaluated in the ambulatory neurology faculty practice at the Icahn School of Medicine at Mount Sinai in New York between January 1 and June 30, 2019. We extracted clinical and demographic characteristics, total office visits, secure MyChart portal messages, and telephone encounters from our institutional data warehouse. We defined high RC and MyChart utilization as the top tertile of RC and MyChart message volume, respectively, and assessed the relationship between patient characteristics and high RC (primary outcome), as well as high MyChart utilization (secondary outcome). We characterized the relationship between clinicodemographic characteristics and the ratio of MyChart messages to total RCs (secondary outcome). Results We identified 1390 patients, of whom 477 (34.3%) were high RC utilizers and 321 (23.1%) were high MyChart utilizers. High RC utilizers generated 3306/3921 (84.3%) RCs. The presence of chronic headache (aOR 2.31, 95% CI 1.75–3.03, p   〈  0.0001), cluster headache (aOR 18.3, 95% CI 5.0–71.7, p  = 0.001), and migraine (aOR 3.82, 95% CI 1.93–9.3, p  = 0.011) was associated with high RC utilization. Patients ≥65 years of age were less likely to engage in MyChart messaging as a proportion of RC (191/680, 28.1%) compared with patients 18–30 years of age (243/620, 39.2%, p  = 0.049) and 30–64 years of age (1172/2721, 43.1%, p   〈  0.0001). Conclusions A minority of patients with headache (477/1390; 34.3%) generated the majority (3306/3921; 84.3%) of RCs. Our findings should be validated in external patient cohorts with the objective of developing strategies to optimize RC utilization.
    Type of Medium: Online Resource
    ISSN: 0017-8748 , 1526-4610
    URL: Issue
    URL: Article
    DOI: 10.1111/head.v61.10
    DOI: 10.1111/head.14226
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2020316-0
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  • 10
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    Postural orthostatic tachycardia syndrome and migraine: A narrative review
    Wiley ; 2022
    In:  Headache: The Journal of Head and Face Pain Vol. 62, No. 7 ( 2022-07), p. 792-800
    Details
    In: Headache: The Journal of Head and Face Pain, Wiley, Vol. 62, No. 7 ( 2022-07), p. 792-800
    Abstract: In this narrative review, we summarize experimental and clinical evidence demonstrating mechanistic connections between POTS and migraine. Background Migraine is the most common comorbidity in patients with POTS, a heterogenous disorder of the autonomic nervous system characterized by orthostatic intolerance and positional tachycardia. POTS is a debilitating illness with few effective treatments. We aim for this narrative review to increase awareness of the mechanistic connections between POTS and migraine providing foundational information that optimizes clinical care and advances the development of pathophysiologic‐based treatments. Methods We used the PubMed and Medline databases in November 2021 to perform a literature review and searched for the following keywords: “postural orthostatic tachycardia syndrome,” “POTS,” “autonomic nervous system,” AND “migraine,” “headache.” Results The high prevalence of migraine in patients with POTS may be explained by common pathologic mechanisms. There is evidence that dysregulation of the sympathetic nervous system, alterations in central and peripheral hemodynamics, and central sensitization increase vulnerability to both POTS and migraine. Non‐pharmacologic and pharmacologic treatments that target these shared mechanisms may provide significant benefit for the patient with POTS and migraine. Conclusions Identification of common affected pathways may provide important insight that advances our understanding and treatment of both migraine and POTS.
    Type of Medium: Online Resource
    ISSN: 0017-8748 , 1526-4610
    URL: Issue
    URL: Article
    DOI: 10.1111/head.v62.7
    DOI: 10.1111/head.14365
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2020316-0
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