In:
New Phytologist, Wiley, Vol. 203, No. 2 ( 2014-07), p. 437-448
Abstract:
Cotton ( G ossypium hirsutum ) is the major source of natural textile fibers. Brassinosteroids ( BR s) play crucial roles in regulating fiber development. The molecular mechanisms of BR s in regulating fiber elongation, however, are poorly understood. pagoda1 (pag1) was identified via an activation tagging genetic screen and characterized by genome walking and brassinolide ( BL ) supplementation. RNA ‐Seq analysis was employed to elucidate the mechanisms of PAG 1 in regulating fiber development. pag1 exhibited dwarfism and reduced fiber length due to significant inhibition of cell elongation and expansion. BL treatment rescued its growth and fiber elongation. PAG 1 encodes a homolog of Arabidopsis CYP 734A1 that inactivates BR s via C‐26 hydroxylation. RNA ‐Seq analyses showed that the constitutive expression of PAG 1 downregulated the expression of genes involved in very‐long‐chain fatty acids ( VLCFA ) biosynthesis, ethylene‐mediated signaling, response to cadmium, cell wall development, cytoskeleton organization and cell growth. Our results demonstrate that PAG 1 plays crucial roles in regulating fiber development via controlling the level of endogenous bioactive BR s, which may affect ethylene signaling cascade by mediating VLCFA . Therefore, BR may be a critical regulator of fiber elongation, a role which may in turn be linked to effects on VLCFA biosynthesis, ethylene and cadmium signaling, cell wall‐ and cytoskeleton‐related gene expression.
Type of Medium:
Online Resource
ISSN:
0028-646X
,
1469-8137
DOI:
10.1111/nph.2014.203.issue-2
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
208885-X
detail.hit.zdb_id:
1472194-6
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