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  • Wiley  (4)
  • 1
    Online Resource
    Online Resource
    Construction of circ_0071922‐miR‐15a‐5p‐mRNA network in intervertebral disc degeneration by RNA‐sequencing
    Wiley ; 2024
    In:  JOR SPINE Vol. 7, No. 1 ( 2024-03)
    Details
    In: JOR SPINE, Wiley, Vol. 7, No. 1 ( 2024-03)
    Abstract: Low back pain (LBP) is the main factor of global disease burden. Intervertebral disc degeneration (IVDD) has long been known as the leading reason of LBP. Increasing studies have verified that circular RNAs (circRNAs)‐microRNAs (miRNAs)‐mRNAs network is widely involved in the pathological processes of IVDD. However, no study was made to demonstrate the circRNAs‐mediated ferroptosis, oxidative stress, extracellular matrix metabolism, and immune response in IVDD. Methods We collected 3 normal and 3 degenerative nucleus pulposus tissues to conduct RNA‐sequencing to identify the key circRNAs and miRNAs in IVDD. Bioinformatics analysis was then conducted to construct circRNAs‐miRNAs‐mRNAs interaction network associated with ferroptosis, oxidative stress, extracellular matrix metabolism, and immune response. We also performed animal experiments to validate the therapeutic effects of key circRNAs in IVDD. Results We found that circ_0015435 was most obviously upregulated and circ_0071922 was most obviously downregulated in IVDD using RNA‐sequencing. Then we observed that hsa‐miR‐15a‐5p was the key downstream of circ_0071922, and hsa‐miR‐15a‐5p was the top upregulated miRNA in IVDD. Bioinformatics analysis was conducted to predict that 56 immunity‐related genes, 29 ferroptosis‐related genes, 23 oxidative stress‐related genes and 8 ECM‐related genes are the targets mRNAs of hsa‐miR‐15a‐5p. Then we constructed a ceRNA network encompassing 24 circRNAs, 6 miRNAs, and 101 mRNAs. Additionally, we demonstrated that overexpression of circ_0071922 can alleviate IVDD progression in a rat model. Conclusions The findings of this study suggested that circ_0071922‐miR‐15a‐5p‐mRNA signaling network might affect IVDD by modulating the nucleus pulposus cells ferroptosis, oxidative stress, ECM metabolism, and immune response, which is an effective therapeutic targets of IVDD.
    Type of Medium: Online Resource
    ISSN: 2572-1143 , 2572-1143
    URL: Issue
    URL: Article
    DOI: 10.1002/jsp2.v7.1
    DOI: 10.1002/jsp2.1275
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 2931784-8
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  • 2
    Online Resource
    Online Resource
    Evolutionary analysis on structural characteristics of water resource system in basins of Northern China
    Wiley ; 2020
    In:  Sustainable Development Vol. 28, No. 4 ( 2020-07), p. 800-812
    Details
    In: Sustainable Development, Wiley, Vol. 28, No. 4 ( 2020-07), p. 800-812
    Abstract: The overdevelopment of water resources has caused the deterioration of the river ecology and environment, resulting in increasing water shortages. This has become one of the key factors that restricts the sustainable development of the social economy and ecological environment of the region. The water network system of the Songhua, Liaohe, Yellow, and Haihe river basins is analyzed by using ecological network analysis for the period 2004–2017. According to our findings, the network structures of various river basins present a relevant gap from its equilibrium value; thus, the water resources have not reached the state of sustainable use. By comparing the value of the water system in Songliao, Yellow, and Haihe river basins with a opt , we found that only the water network structure of the Songliao River Basin is close to the point of equilibrium. Therefore, we recommend to improve water‐use efficiency and reduce production and domestic water consumption by adjusting water prices, carrying out agricultural water‐saving irrigation, and increasing the use of industrial circulating water. This set of measures would ensure sufficient ecological water consumption in the basin, in order to meet the needs of a healthy and sustainable development of the ecological environment.
    Type of Medium: Online Resource
    ISSN: 0968-0802 , 1099-1719
    URL: Issue
    URL: Article
    DOI: 10.1002/sd.v28.4
    DOI: 10.1002/sd.2031
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2021120-X
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  • 3
    Online Resource
    Online Resource
    The association between low virulence organisms in different locations and intervertebral disc structural failure: A meta‐analysis and systematic review
    Wiley ; 2023
    In:  JOR SPINE Vol. 6, No. 2 ( 2023-06)
    Details
    In: JOR SPINE, Wiley, Vol. 6, No. 2 ( 2023-06)
    Abstract: Many factors may trigger intervertebral disc (IVD) structural failure (intervertebral disc degeneration (IDD) and endplate changes), including inflammation, infection, dysbiosis, and the downstream effects of chemical factors. Of these, microbial diversity in the IVD and elsewhere in the body has been considered as one of the potential reasons for disc structural failure. The exact relationships between microbial colonization and IVD structural failure are not well understood. This meta‐analysis aimed to investigate the impact of microbial colonization and its location (such as skin, IVD, muscle, soft tissues, and blood) on IVD structural failure and corresponding low back pain (LBP) if any. We searched four online databases for potential studies. The potential relationships between microbial colonization in different sample sources (such as skin, IVD, muscle, soft tissues, and blood) and IDD and endplate change were considered as primary outcomes. Odds ratio (OR) and 95% confidence intervals (CI) for direct comparisons were reported. Grading of Recommendations Assessment, Development and Evaluation (GRADE) scale was used to assess the quality of evidence. Twenty‐five cohort studies met the selection criteria. Overall pooled prevalence of microbial colonization in 2419 patients with LBP was 33.2% (23.6%–43.6%). The pooled prevalence of microbial colonization in 2901 samples was 29.6% (21.0%–38.9%). Compared with the patients without endplate change, the patients with endplate changes had higher rates of microbial colonization of disc (OR = 2.83; 95% CI = 1.93–4.14; I 2  = 37.6%; p  = 0.108). The primary pathogen was Cutibacterium acnes which was present in 22.2% of cases (95% CI = 13.3%–32.5%; I 2  = 96.6%; p  = 0.000). This meta‐analysis and systematic review found low‐quality grade evidence for an association between microbial colonization of disc with endplate changes. The primary pathogen was C. acnes . Due to lack of enough high‐quality studies and methodological limitations of this review, further studies are required to improve our understanding of the potential relationships and mechanisms of microbiota, dysbiosis, IVD colonization and IVD structural failure.
    Type of Medium: Online Resource
    ISSN: 2572-1143 , 2572-1143
    URL: Issue
    URL: Article
    DOI: 10.1002/jsp2.v6.2
    DOI: 10.1002/jsp2.1244
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2931784-8
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  • 4
    Online Resource
    Online Resource
    Links among MRI features in paraspinal muscles, inflammatory processes, and related back pain in patients with lumbar disc herniation
    Wiley ; 2024
    In:  JOR SPINE Vol. 7, No. 1 ( 2024-03)
    Details
    In: JOR SPINE, Wiley, Vol. 7, No. 1 ( 2024-03)
    Abstract: Recent studies have provided evidence that structural changes in paraspinal muscles are associated with intervertebral disc degeneration (IDD), ubiquitous with low back pain (LBP), and potentially thought to be regulated by inflammatory processes. However, the links remain unclear. Objective The aims of this study were to investigate structural changes in paraspinal muscles that differed in healthy and lumbar disc herniation (LDH) patients, and LDH patients with and without LBP, and to determine the link with the expression of inflammatory marker(s). Methods Cross‐sectional areas (CSAs) and fatty degeneration of muscles were measured in this prospective cohort study. Multifidus muscle (MM) tissue was procured from included individuals undergoing surgery. Gene expression was quantified using qPCR assays. Independent t ‐test, Chi‐square, and Spearman correlation were used for evaluating the links among structural changes, expression of inflammatory markers, and clinical outcomes. Results Functional CSA and fatty degeneration of MM were larger in healthy group than LDH group. A significant increase in fat infiltration in MM in LBP group than in non‐LBP group. TNF‐alpha (TNF‐α) was 28‐fold greater in high‐fat infiltration group than low‐fat infiltration group within MM. Expression of TNF‐α and IL‐1β in MM was moderately correlated with functional CSA and fatty degeneration of MM, which was moderately correlated with clinical outcomes. Conclusions Results support the hypothesis that IDD is associated with dysregulation of inflammatory state of local MM, which provides initial evidence that inflammatory dysregulation in paraspinal muscles has the potential for a broad impact on tissue health and LBP symptoms.
    Type of Medium: Online Resource
    ISSN: 2572-1143 , 2572-1143
    URL: Issue
    URL: Article
    DOI: 10.1002/jsp2.v7.1
    DOI: 10.1002/jsp2.1310
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 2931784-8
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