In:
Journal of Phytopathology, Wiley, Vol. 145, No. 5-6 ( 1997-06), p. 235-238
Abstract:
A polymerase chain reaction (PCR)‐based assay was used to detect pelargonium flower break carmovirus (PFBV) in total RNA extractions made from infected Pelargonium plants. Extracts were reverse transcribed (RT) and the resultant cDNA was amplified by PCR, using oligonucleotide primers specific for 343, 510 and 832 base pair fragments of the RNA‐dependent RNA polymerase gene of PFBV. The specificity and sensitivity of RT‐PCR were compared with the enzyme‐linked immunosorbent assay (ELISA) for the detection of PFBV in Pelargonium tissues. The virus could be detected efficiently in high dilutions of sap from infected plants and at low concentrations of purified virus. Although ELISA is a powerful tool for virus detection, RT‐PCR was over 1000 times more sensitive in detecting PFBV in leaf extracts of infected Pelargonium than was ELISA. The limit of detecting PFBV RNA by RT‐PCR was 200 fg, compared with 200 pg of virus by ELISA.
Type of Medium:
Online Resource
ISSN:
0931-1785
,
1439-0434
DOI:
10.1111/jph.1997.145.issue-5-6
DOI:
10.1111/j.1439-0434.1997.tb00392.x
Language:
English
Publisher:
Wiley
Publication Date:
1997
detail.hit.zdb_id:
2020539-9
SSG:
12
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