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  • 1
    Online Resource
    Online Resource
    In Vitro Studies of Composite Bone Filler Based on Poly(Propylene Fumarate) and Biphasic α‐Tricalcium Phosphate/Hydroxyapatite Ceramic Powder
    Wiley ; 2012
    In:  Artificial Organs Vol. 36, No. 4 ( 2012-04), p. 418-428
    Details
    In: Artificial Organs, Wiley, Vol. 36, No. 4 ( 2012-04), p. 418-428
    Abstract: While many different filler materials have been applied in vertebral augmentation procedures, none is perfect in all biomechanical and biological characteristics. To minimize possible shortages, we synthesized a new biodegradable, injectable, and premixed composite made from poly(propylene fumarate) (PPF) and biphasic α‐tricalcium phosphate (α‐TCP)/hydroxyapatite (HAP) ceramics powder and evaluated the material properties of the compound in vitro. We mixed the PPF cross‐linked by N‐vinyl pyrrolidinone and biphasic α‐TCP/HAP powder in different ratios with benzoyl peroxide as an initiator. The setting time and temperature were recorded, although they could be manipulated by modulating the concentrations of hydroquinone and N,N‐dimethyl‐p‐toluidine. Degradation, cytocompatibility, mechanical properties, and radiopacity were analyzed after the composites were cured by a cylindrical shape. We also compared the study materials with poly(methyl methacrylate) (PMMA) and PPF with pure HAP particles. Results showed that lower temperature during curing process (38–44°C), sufficient initial mechanical compressive fracture strength (61.1 ± 3.7 MPa), and gradual degradation were observed in the newly developed bone filler. Radiopacity in Hounsfield units was similar to PMMA as determined by computed tomography scan. Both pH value variation and cytotoxicity were within biological tolerable limits based on the biocompatibility tests. Mixtures with 70% α‐TCP/HAP powder were superior to other groups. This study indicated that a composite of PPF and biphasic α‐TCP/HAP powder is a promising, premixed, injectable biodegradable filler and that a mixture containing 70% α‐TCP/HAP exhibits the best properties.
    Type of Medium: Online Resource
    ISSN: 0160-564X , 1525-1594
    URL: Issue
    URL: Article
    DOI: 10.1111/aor.2012.36.issue-4
    DOI: 10.1111/j.1525-1594.2011.01372.x
    Language: English
    Publisher: Wiley
    Publication Date: 2012
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  • 2
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    Tissue engineering‐based cartilage repair with mesenchymal stem cells in a porcine model
    Wiley ; 2011
    In:  Journal of Orthopaedic Research Vol. 29, No. 12 ( 2011-12), p. 1874-1880
    Details
    In: Journal of Orthopaedic Research, Wiley, Vol. 29, No. 12 ( 2011-12), p. 1874-1880
    Abstract: This in vivo pilot study explored the use of mesenchymal stem cell (MSC) containing tissue engineering constructs in repair of osteochondral defects. Osteochondral defects were created in the medial condyles of both knees of 16 miniature pigs. One joint received a cell/collagen tissue engineering construct with or without pretreatment with transforming growth factor β (TGF‐β) and the other joint from the same pig received no treatment or the gel scaffold only. Six months after surgery, in knees with no treatment, all defects showed contracted craters; in those treated with the gel scaffold alone, six showed a smooth gross surface, one a hypertrophic surface, and one a contracted crater; in those with undifferentiated MSCs, five defects had smooth, fully repaired surfaces or partially repaired surfaces, and one defect poor repair; in those with TGF‐β‐induced differentiated MSCs, seven defects had smooth, fully repaired surfaces or partially repaired surfaces, and three defects showed poor repair. In Pineda score grading, the group with undifferentiated MSC, but not the group with TGF‐β‐induced differentiated MSCs, had significantly lower subchondral, cell morphology, and total scores than the groups with no or gel‐only treatment. The compressive stiffness was larger in cartilage without surgical treatment than the treated area within each group. In conclusion, this preliminary pilot study suggests that using undifferentiated MSCs might be a better approach than using TGF‐β‐induced differentiated MSCs for in vivo tissue engineered treatment of osteochondral defects. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29:1874–1880, 2011
    Type of Medium: Online Resource
    ISSN: 0736-0266 , 1554-527X
    URL: Issue
    URL: Article
    DOI: 10.1002/jor.v29.12
    DOI: 10.1002/jor.21461
    Language: English
    Publisher: Wiley
    Publication Date: 2011
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    detail.hit.zdb_id: 2050452-4
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  • 3
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    Therapeutic potential of nanoceria pretreatment in preventing the development of urological chronic pelvic pain syndrome: Immunomodulation via reactive oxygen species scavenging and SerpinB2 downregulation
    Wiley ; 2023
    In:  Bioengineering & Translational Medicine Vol. 8, No. 1 ( 2023-01)
    Details
    In: Bioengineering & Translational Medicine, Wiley, Vol. 8, No. 1 ( 2023-01)
    Abstract: Urological chronic pelvic pain syndrome (UCPPS) manifests as pelvic pain with frequent urination and has a 10% prevalence rate without effective therapy. Nanoceria (cerium oxide nanoparticles [CNPs]) were synthesized in this study to achieve potential long‐term pain relief, using a commonly used UCPPS mouse model with cyclophosphamide‐induced cystitis. Transcriptome sequencing analysis revealed that serpin family B member 2 (SerpinB2) was the most upregulated marker in mouse bladder, and SerpinB2 was downregulated with CNP pretreatment. The transcriptome sequencing analysis results agreed with quantitative polymerase chain reaction and western blot analysis results for the expression of related mRNAs and proteins. Analysis of Gene Expression Omnibus (GEO) datasets revealed that SerpinB2 was a differentially upregulated gene in human UCPPS. In vitro SerpinB2 knockdown downregulated proinflammatory chemokine expression (chemokine receptor CXCR3 and C‐X‐C motif chemokine ligand 10) upon treatment with 4‐hydroperoxycyclophosphamide. In conclusion, CNP pretreatment may prevent the development of UCPPS, and reactive oxygen species (ROS) scavenging and SerpinB2 downregulation may modulate the immune response in UCPPS.
    Type of Medium: Online Resource
    ISSN: 2380-6761 , 2380-6761
    URL: Issue
    URL: Article
    DOI: 10.1002/btm2.v8.1
    DOI: 10.1002/btm2.10346
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2865162-5
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  • 4
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    Online Resource
    Development of di(2‐ethylhexyl) phthalate‐containing thioglycolic acid immobilized chitosan mucoadhesive gel as an alternative hormone therapy for menopausal syndrome
    Wiley ; 2022
    In:  Bioengineering & Translational Medicine Vol. 7, No. 2 ( 2022-05)
    Details
    In: Bioengineering & Translational Medicine, Wiley, Vol. 7, No. 2 ( 2022-05)
    Abstract: Menopausal syndrome includes the symptoms that most women experience owing to hormone changes after menopause. Although hormone replacement therapy is a common treatment for menopausal syndrome, there are still many side effects and challenges hindering research. In this study, thioglycolic acid (TGA)‐immobilized chitosan mucoadhesive gel was synthesized by a new method of low concentration of 1,4‐butanediol diglycidyl ether (BDDE) would encapsulate di(2‐ethylhexyl) phthalate (DEHP) as an alternative hormone replacement therapy for menopausal syndrome. The efficacies of the DEHP‐containing TGA‐chitosan gel (CT‐D) were confirmed and evaluated by materials characterization and in vitro study. Results showed that CT‐D was not cytotoxic and had better mucoadhesive ability than chitosan. The animal model was constructed 1 month after bilateral ovariectomy in SD rats. CT‐D was administered intravaginally every 3 days. Bodyweight, wet weight of the uterus and vagina, vaginal smears, histology, blood element analysis, and serological analysis was used to assess the ability of the material to relieve menopausal syndrome. The results indicated that the combination of the sustained release of DEHP and mucoadhesive TGA‐immobilized chitosan allows the developed CT‐D to relieve the menopausal syndrome through low concentrations of DEHP, which falls in the safety level of the tolerable daily intake of DEHP.
    Type of Medium: Online Resource
    ISSN: 2380-6761 , 2380-6761
    URL: Issue
    URL: Article
    DOI: 10.1002/btm2.v7.2
    DOI: 10.1002/btm2.10267
    Language: English
    Publisher: Wiley
    Publication Date: 2022
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  • 5
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    Online Resource
    The combination of resveratrol and Bletilla striata polysaccharide decreases inflammatory markers of early osteoarthritis knee and the preliminary results on LPS ‐induced OA rats
    Wiley ; 2023
    In:  Bioengineering & Translational Medicine Vol. 8, No. 4 ( 2023-07)
    Details
    In: Bioengineering & Translational Medicine, Wiley, Vol. 8, No. 4 ( 2023-07)
    Abstract: Osteoarthritis (OA) of the knee is characterized by progressive deterioration and loss of articular cartilage with associatedstructural and performance changes in the entire joint, and current treatments for OA only aim to relieve symptoms, rather than to prevent or reverse disease progression. Recently, treatments targeting “early osteoarthritis” (EOA) have attracted attention. However, during EOA stage, chondrocytes may change behaviors to express pro‐inflammatory cytokines and free radicals, which would cause detrimental effects to the synovial cavity and further cartilage wear. In this study, we combined resveratrol (Res) and Bletilla striata polysaccharide (BSP) as anti‐inflammatories and antioxidants to diffuse free radicals and to alleviate inflammation from the synovial cavity both short term and long term. The current study introduced a new method for harvesting BSP from as‐received Bletilla striata to achieve high yields, shortened extraction times, and maintained structure/functions. In addition, it combined Res and home‐extracted BSP (Res‐BSP) to alleviate oxidative stress and inflammation in a Lipopolysaccharide (LPS)‐induced OA model. The gene expressions of inflammatory genes iNOs, IL‐1β, IL‐6, and MMP‐13 were upregulated 5.7‐fold, 6.5‐fold, 8.6‐fold, and 4.5‐fold, respectively on OA‐like chondrocytes and the gene expressions were significantly downregulated to 3.3‐fold, 2.1‐fold, 4.9‐fold, and 0.1‐fold, respectively, once OA‐like chondrocytes were treated with Res‐BSP ( p   〈  0.05, compared with OA‐like chondrocytes). The gene expressions of chondrogenic genes TGFβ1, SOX9, and type II collagen were downregulated by 0.8‐fold, 2.2‐fold, and 0.8‐fold, respectively, based on the control group as a baseline. While it was significantly upregulated by 3.4‐fold, 0.32‐fold, and 0.4‐fold, respectively, once OA‐like chondrocytes were treated with Res‐BSP. ( p   〈  0.05, compared with OA‐like chondrocytes). Finally, we elucidated the role of Res‐BSP in EOA in suppressing COX‐2 and activating p‐Smad 2/3 and p‐Erk1/2. We believe that the combination of Res and BSP has great potential as an alternative therapeutic strategy for EOA treatment in future.
    Type of Medium: Online Resource
    ISSN: 2380-6761 , 2380-6761
    URL: Issue
    URL: Article
    DOI: 10.1002/btm2.v8.4
    DOI: 10.1002/btm2.10431
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2865162-5
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  • 6
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    Preparation of ?TCP/HAP biphasic ceramics with natural bone structure by heating bovine cancellous bone with the addition of (NH4)2HPO4
    Wiley ; 2000
    In:  Journal of Biomedical Materials Research Vol. 51, No. 2 ( 2000-08), p. 157-163
    Details
    In: Journal of Biomedical Materials Research, Wiley, Vol. 51, No. 2 ( 2000-08), p. 157-163
    Type of Medium: Online Resource
    ISSN: 0021-9304 , 1097-4636
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.1002/(SICI)1097-4636(200008)51:2〈〉1.0.CO;2-W
    DOI: 10.1002/(ISSN)1097-4636
    DOI: 10.1002/(SICI)1097-4636(200008)51:2〈157::AID-JBM3〉3.0.CO;2-R
    Language: English
    Publisher: Wiley
    Publication Date: 2000
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    detail.hit.zdb_id: 280321-5
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  • 7
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    Chondrogenesis From Immortalized Human Mesenchymal Stem Cells: Comparison Between Collagen Gel and Pellet Culture Methods
    Wiley ; 2008
    In:  Artificial Organs Vol. 32, No. 7 ( 2008-07), p. 561-566
    Details
    In: Artificial Organs, Wiley, Vol. 32, No. 7 ( 2008-07), p. 561-566
    Abstract: Abstract:  Human mesenchymal stem cells (hMSCs) can differentiate into cells of connective tissue lineages, including cartilage. To overcome the limiting autogenous chondrocyte populations available in cartilage repair, various methods have been developed to maximize chondrogenesis of hMSCs in vitro, most of which use cells derived from primary culture. In this study, we compared chondrogenesis of immortalized hMSCs embedded in collagen gel to those grown in pellet culture. The hMSCs in collagen scaffolds expressed more glycosaminoglycan than those in pellet culture. Real‐time reverse transcriptase–polymerase chain reaction (RT–PCR) analysis demonstrated that the expression of genes encoding sox‐9 , aggrecan, and types I and II collagen increased in pellet culture over time. However, in the collagen cultures, only type II collagen and aggrecan expression increased over time, whereas sox‐9 expression remained unchanged and type I collagen expression decreased. These results indicate that the immortalized hMSC line is a promising tool for further in vitro chondrogenic studies.
    Type of Medium: Online Resource
    ISSN: 0160-564X , 1525-1594
    URL: Issue
    URL: Article
    DOI: 10.1111/aor.2008.32.issue-7
    DOI: 10.1111/j.1525-1594.2008.00575.x
    Language: English
    Publisher: Wiley
    Publication Date: 2008
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    detail.hit.zdb_id: 2003825-2
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  • 8
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    Influence of age‐related degeneration on regenerative potential of human nucleus pulposus cells
    Wiley ; 2010
    In:  Journal of Orthopaedic Research Vol. 28, No. 3 ( 2010-03), p. 379-383
    Details
    In: Journal of Orthopaedic Research, Wiley, Vol. 28, No. 3 ( 2010-03), p. 379-383
    Abstract: Nucleus pulposus (NP) cells, sourced from herniation surgeries, may be used as a cell‐based therapy for intervertebral disc (IVD) degeneration. But, both the regenerative potential of these degenerative adult NP cells and how to stimulate optimum matrix synthesis is not yet clear. The purpose of the current study was to understand the different phenotypic behaviors between degenerative adult NP cells and normal adolescent NP cells. Degenerative adult NP cells produced a significantly higher amount of proteoglycans and collagens than adolescent cells. Insulin‐like growth factor‐1 was the only anabolic cytokine with increased endogenous expression in degenerative adult NP cells. TGF‐β1 treatment of degenerative NP cells promoted matrix synthesis but stimulated too much type I collagen and suppressed type II collagen and aggrecan. Adult degenerative NP cells possess upregulated regenerative potential, but stimulation in addition to TGF‐β1 is needed to enhance matrix productivity and optimize the collagen expression profile. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:379–383, 2010
    Type of Medium: Online Resource
    ISSN: 0736-0266 , 1554-527X
    URL: Issue
    URL: Article
    DOI: 10.1002/jor.v28:3
    DOI: 10.1002/jor.20988
    Language: English
    Publisher: Wiley
    Publication Date: 2010
    detail.hit.zdb_id: 605542-4
    detail.hit.zdb_id: 2050452-4
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  • 9
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    Regeneration of dentin‐pulp complex with cementum and periodontal ligament formation using dental bud cells in gelatin‐chondroitin‐hyaluronan tri‐copolymer scaffold in swine
    Wiley ; 2008
    In:  Journal of Biomedical Materials Research Part A Vol. 86A, No. 4 ( 2008-09-15), p. 1062-1068
    Details
    In: Journal of Biomedical Materials Research Part A, Wiley, Vol. 86A, No. 4 ( 2008-09-15), p. 1062-1068
    Abstract: The purpose of this study is to use a tissue engineering approach for tooth regeneration. The swine dental bud cells (DBCs) were isolated from the developing mandibular teeth, expanded in vitro , and cultured onto cylinder scaffold gelatin‐chrondroitin‐hyaluronan‐tri‐copolymer (GCHT). After culturing in vitro , the DBCs/GCHT scaffold was autografted back into the original alveolar socket. Hematoxylin and eosin (H & E) staining combined with immunohistochemical staining were applied for identification of regenerated tooth structure. After 36‐week post‐transplantation, tooth‐like structures, including well‐organized dentin‐pulp complex, cementum, and periodontal ligament, were evident in situ in two of six experimental animals. The size of the tooth structure (1 × 0.5 × 0.5 cm 3 and 0.5 × 0.5 × 0.5 cm 3 size) appeared to be dictated by the size of the GCHT scaffold (1 × 1 × 1.5 cm 3 ). The third swine was demonstrated with irregular dentin‐bony like calcified tissue about 1 cm in diameter without organized tooth or periodontal ligament formation. The other three swine in the experimental group showed normal bone formation and no tooth regeneration in the transplantation sites. The successful rate of tooth regeneration from DBCs/GCHT scaffolds' was about 33.3%. In the control group, three swine's molar teeth buds were removed without DBCs/GCHT implantation, the other three swine received GCHT scaffold implants without DBCs. After evaluation, no regenerated tooth was found in the transplantation site of the control group. The current results using DBSs/GCHT scaffold autotransplantation suggest a technical breakthrough for tooth regeneration. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008
    Type of Medium: Online Resource
    ISSN: 1549-3296 , 1552-4965
    URL: Issue
    URL: Article
    DOI: 10.1002/jbm.a.v86a:4
    DOI: 10.1002/jbm.a.31746
    Language: English
    Publisher: Wiley
    Publication Date: 2008
    detail.hit.zdb_id: 1477192-5
    detail.hit.zdb_id: 2099989-6
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  • 10
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    Nano‐sized collagen I molecules enhanced the differentiation of rat mesenchymal stem cells into cardiomyocytes
    Wiley ; 2013
    In:  Journal of Biomedical Materials Research Part A Vol. 101, No. 10 ( 2013-10), p. 2808-2816
    Details
    In: Journal of Biomedical Materials Research Part A, Wiley, Vol. 101, No. 10 ( 2013-10), p. 2808-2816
    Abstract: The aim of this study was to investigate the ability of nano‐sized collagen I molecules (nanoparticles or nanofibrils) and a 5‐azacytidine (5‐aza) treatment to enhance the differentiation of rat mesenchymal stem cells (MSCs) toward a cardiomyogenic phenotype in vitro . Second passaged MSCs were cocultured with nano‐sized collagen I molecules for 24 h and then treated with 10 μ M 5‐aza for 24 h. The results demonstrated that the size of the cells increased significantly and acquired a flattened, triangular‐shaped morphology after treatment with nano‐sized collagen I molecules and 5‐aza. The cells are connecting with adjoining cells by forming myotube‐like structures. Additional treatment of the MSCs with nano‐sized collagen I fibrils significantly increased two transcription factors GATA‐4 (12.6‐fold increase) and Nkx2.5 (4.8‐fold increase) expressions compared with MSC groups treated only with 5‐aza at 3‐day culturing. Furthermore, MSCs pretreated with nano‐sized collagen fibrils significantly increased the expressions of cardiac genes of troponin I, β‐myosin heavy chain, and cardiac α‐actin compared with MSC groups treated only with 5‐aza (all, p 〈 0.01 or better). These results indicate that culturing MSCs with nano‐sized collagen I molecules, which may act as scaffolds or soluble protein ingredients, leads to alterations in gene expression and affects the differentiation fate induced with 5‐aza. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 2808–2816, 2013.
    Type of Medium: Online Resource
    ISSN: 1549-3296 , 1552-4965
    URL: Issue
    URL: Article
    DOI: 10.1002/jbm.a.v101.10
    DOI: 10.1002/jbm.a.34589
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 1477192-5
    detail.hit.zdb_id: 2099989-6
    SSG: 12
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