In:
European Journal of Immunology, Wiley, Vol. 39, No. 8 ( 2009-08), p. 2203-2214
Abstract:
The vascular addressins mucosal addressin cell adhesion molecule‐1, P‐selectin and ICAM‐1 permit α 4 β 7 ‐integrin‐expressing DC, especially those of the myeloid lineage (CD11c + CD11b + DC), to access the pregnant mouse uterus. Injection of blocking monoclonal antibodies against mucosal addressin cell adhesion molecule‐1 in P‐selectin −/− mice or experimental approaches with β7‐integrin −/− or ICAM‐1 −/− mice revealed that limited access or absence of CD11c + CD11b + DC at the maternal/fetal interface negatively affects the frequency, size and functional properties of uterine NK (uNK) cells. Adoptive transfer of DC obtained from WT mice into β7‐integrin −/− mice abrogates these effects and emphasizes the importance of DC in uNK cell differentiation. Interestingly, those implantation sites lacking CD11c + CD11b + DC are characterized by decreased IL‐15 and IL‐12 mRNA and/or protein levels. Chronic administration of IL‐15 in these mice gives rise to uNK cell numbers and size comparable to those of WT mice, whereas additional injection of IL‐12 positively affects the IFN‐γ expression of uNK cells. Real‐time RT‐PCR and protein arrays performed with isolated uterine DC underline the role of DC as a source of IL‐15 and IL‐12 in the pregnant mouse uterus.
Type of Medium:
Online Resource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.200838844
Language:
English
Publisher:
Wiley
Publication Date:
2009
detail.hit.zdb_id:
1491907-2
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