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  • 1
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    A serum lipidomics study for the identification of specific biomarkers for endometrial polyps to distinguish them from endometrial cancer or hyperplasia
    Wiley ; 2022
    In:  International Journal of Cancer Vol. 150, No. 9 ( 2022-05), p. 1549-1559
    Details
    In: International Journal of Cancer, Wiley, Vol. 150, No. 9 ( 2022-05), p. 1549-1559
    Abstract: Endometrial diseases, including endometrial polyps (EP), endometrial cancer (EC) and endometrial hyperplasia (EH), are common gynecological diseases that affect women of childbearing and perimenopausal age. Clinically, biopsy or imaging methods are usually used to screen and diagnose these diseases; however, due to the invasiveness and heterogeneity of these tests, a noninvasive, convenient, objective and accurate biomarker is needed for the differential diagnosis of EP, EC or EH. In the present study, serum samples from 326 patients with endometrial diseases and 225 healthy volunteers were analyzed using nontargeted lipidomics. A combination of multivariate and univariate analyses was used to identify and qualify six, eight and seven potential biomarkers in the sera from patients with EP, EC and EH, respectively. Using a logistic regression algorithm and receiver operating characteristic (ROC) curve analysis, a biomarker panel including four specific EP biomarkers, 6‐keto‐PGF1α, PA(37:4), LysoPC(20:1) and PS(36:0), showed good classification and diagnostic ability in distinguishing EP from EC or EH. The biomarker panel for distinguishing EP from EC yielded an area under the curve (AUC) of 0.915, sensitivity of 100% and specificity of 72.41%, while that for distinguishing EP from EH yielded an AUC of 1.000, sensitivity of 100% and specificity of 100%. The two diagnostic models also showed good diagnostic abilities in the validation set. Therefore, this biomarker panel can be used as a rapid diagnostic method to assist in imaging examinations and provide a reference for clinicians in the identification and diagnosis of endometrial diseases.
    Type of Medium: Online Resource
    ISSN: 0020-7136 , 1097-0215
    URL: Issue
    URL: Article
    DOI: 10.1002/ijc.v150.9
    DOI: 10.1002/ijc.33943
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 218257-9
    detail.hit.zdb_id: 1474822-8
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  • 2
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    Impact of individual factors on DNA methylation of drug metabolism genes: A systematic review
    Wiley ; 2023
    In:  Environmental and Molecular Mutagenesis Vol. 64, No. 7 ( 2023-08), p. 401-415
    Details
    In: Environmental and Molecular Mutagenesis, Wiley, Vol. 64, No. 7 ( 2023-08), p. 401-415
    Abstract: Individual differences in drug response have always existed in clinical treatment. Many non‐genetic factors show non‐negligible impacts on personalized medicine. Emerging studies have demonstrated epigenetic could connect non‐genetic factors and individual treatment differences. We used systematic retrieval methods and reviewed studies that showed individual factors’ impact on DNA methylation of drug metabolism genes. In total, 68 studies were included, and half ( n  = 36) were cohort studies. Six aspects of individual factors were summarized from the perspective of personalized medicine: parental exposure, environmental pollutants exposure, obesity and diet, drugs, gender and others. The most research ( n  = 11) focused on ABCG1 methylation. The majority of studies showed non‐genetic factors could result in a significant DNA methylation alteration in drug metabolism genes, which subsequently affects the pharmacokinetic processes. However, the underlying mechanism remained unknown. Finally, some viewpoints were presented for future research.
    Type of Medium: Online Resource
    ISSN: 0893-6692 , 1098-2280
    URL: Issue
    URL: Article
    DOI: 10.1002/em.v64.7
    DOI: 10.1002/em.22567
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1497682-1
    SSG: 12
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  • 3
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    Genetic improvement of the shoot architecture and yield in soya bean plants via the manipulation of GmmiR156b
    Wiley ; 2019
    In:  Plant Biotechnology Journal Vol. 17, No. 1 ( 2019-01), p. 50-62
    Details
    In: Plant Biotechnology Journal, Wiley, Vol. 17, No. 1 ( 2019-01), p. 50-62
    Abstract: The optimization of plant architecture in order to breed high‐yielding soya bean cultivars is a goal of researchers. Tall plants bearing many long branches are desired, but only modest success in reaching these goals has been achieved. Micro RNA 156 ( miR156 ) ‐ SQUAMOSA PROMOTER BINDING PROTEIN ‐ LIKE ( SPL ) gene modules play pivotal roles in controlling shoot architecture and other traits in crops like rice and wheat. However, the effects of miR156 ‐ SPL modules on soya bean architecture and yield, and the molecular mechanisms underlying these effects, remain largely unknown. In this study, we achieved substantial improvements in soya bean architecture and yield by overexpressing GmmiR156b . Transgenic plants produced significantly increased numbers of long branches, nodes and pods, and they exhibited an increased 100‐seed weight, resulting in a 46%–63% increase in yield per plant. Intriguingly, GmmiR156b overexpression had no significant impact on plant height in a growth room or under field conditions; however, it increased stem thickness significantly. Our data indicate that GmmiR156b modulates these traits mainly via the direct cleavage of SPL transcripts. Moreover, we found that Gm SPL 9d is expressed in the shoot apical meristem and axillary meristems ( AM s) of soya bean, and that Gm SPL 9d may regulate axillary bud formation and branching by physically interacting with the homeobox gene WUSCHEL ( WUS ), a central regulator of AM formation. Together, our results identify GmmiR156b as a promising target for the improvement of soya bean plant architecture and yields, and they reveal a new and conserved regulatory cascade involving miR156 ‐ SPL ‐ WUS that will help researchers decipher the genetic basis of plant architecture.
    Type of Medium: Online Resource
    ISSN: 1467-7644 , 1467-7652
    URL: Issue
    URL: Article
    DOI: 10.1111/pbi.2019.17.issue-1
    DOI: 10.1111/pbi.12946
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2136367-5
    SSG: 12
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  • 4
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    Hepatobiliary Tumor Organoids Reveal HLA Class I Neoantigen Landscape and Antitumoral Activity of Neoantigen Peptide Enhanced with Immune Checkpoint Inhibitors
    Wiley ; 2022
    In:  Advanced Science Vol. 9, No. 22 ( 2022-08)
    Details
    In: Advanced Science, Wiley, Vol. 9, No. 22 ( 2022-08)
    Abstract: Neoantigen‐directed therapy lacks preclinical models recapitulating neoantigen characteristics of original tumors. It is urgent to develop a platform to assess T cell response for neoantigen screening. Here, immunogenic potential of neoantigen‐peptides of tumor tissues and matched organoids ( n = 27 pairs) are analyzed by Score tools with whole genome sequencing (WGS)‐based human leukocyte antigen (HLA)‐class‐I algorithms. The comparisons between 9203 predicted neoantigen‐peptides from 2449 mutations of tumor tissues and 9991 ones from 2637 mutations of matched organoids demonstrate that organoids preserved majority of genetic features, HLA alleles, and similar neoantigen landscape of original tumors. Higher neoantigen load is observed in tumors with early stage. Multiomics analysis combining WGS, RNA‐seq, single‐cell RNA‐seq, mass spectrometry filters out 93 candidate neoantigen‐peptides with strong immunogenic potential for functional validation in five organoids. Immunogenic peptides are defined by inducing increased CD107aCD137IFN‐ γ expressions and IFN‐ γ secretion of CD8 cells in flow cytometry and enzyme‐linked immunosorbent assay assays. Nine immunogenic peptides shared by at least two individuals are validated, including peptide from TP53 R90S . Organoid killing assay confirms the antitumor activity of validated immunogenic peptide‐reactive CD8 cells, which is further enhanced in the presence of immune checkpoint inhibitors. The study characterizes HLA‐class‐I neoantigen landscape in hepatobiliary tumor, providing practical strategy with tumor organoid model for neoantigen‐peptide identification in personalized immunotherapy.
    Type of Medium: Online Resource
    ISSN: 2198-3844 , 2198-3844
    URL: Issue
    URL: Article
    DOI: 10.1002/advs.v9.22
    DOI: 10.1002/advs.202105810
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2808093-2
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  • 5
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    Individualized treatment with voriconazole in the Chinese population: inflammation level as a novel marker for dose optimization
    Wiley ; 2023
    In:  British Journal of Clinical Pharmacology
    Details
    In: British Journal of Clinical Pharmacology, Wiley
    Abstract: The aim of this study was to explore the influence and possible mechanisms of pharmacokinetics‐related genes polymorphisms, especially CYP2C19 polymorphisms, and non‐genetic factors combined with the inflammatory status on the voriconazole (VRC) metabolism of the Chinese population. In clinical studies, it was performed with collecting more than one VRC trough concentration and C‐reactive protein (CRP) level. A total of 265 blood samples were collected from 120 patients. Results of multiple regression analyses demonstrated that CYP2C19 genotypes and albumin (Alb) level were remained predictors of C min ss/D in patients with no to mild inflammation ( R 2 = 0.12, P 〈 0.001). In addition, in patients with moderate to severe inflammation, it resulted in a significant model containing factors of CRP and total bilirubin (T‐Bil) levels ( R 2 = 0.19, P 〈 0.001). In non‐clinical studies, 32 rats were divided into control group and inflammatory group, and it was found that the mean residence time (MRT (0‐t) ) of VRC in inflammatory group was significantly longer than that in control group ( P 〈 0.001), which may be due to down‐regulation of mRNA and protein expression of CYP2C19 ( CYP2C6 in rats) through Interleukin (IL)‐6/signal transducer and activator of transcription (STAT) 3 pathway. Therefore, the effect of CYP2C19 polymorphisms on VRC metabolism may be masked by inflammatory status, which should be of more concern than CYP2C19 polymorphisms in patients with moderate to severe inflammation. Additionally, the impact of Alb and T‐Bil on VRC metabolism should not be disregarded.
    Type of Medium: Online Resource
    ISSN: 0306-5251 , 1365-2125
    URL: Article
    DOI: 10.1111/bcp.15916
    RVK:
    XA 33650
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1498142-7
    SSG: 15,3
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  • 6
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    MiR‐9 promotes multiple myeloma progression by regulating TRIM56/NF‐κB pathway
    Wiley ; 2019
    In:  Cell Biology International Vol. 43, No. 11 ( 2019-11), p. 1223-1233
    Details
    In: Cell Biology International, Wiley, Vol. 43, No. 11 ( 2019-11), p. 1223-1233
    Abstract: miR‐9 has been reported to play a pivotal role in multiple human cancers by acting as an oncogene or tumor suppressor. In this study, we explored the possible role and molecular mechanism of miR‐9 in multiple myeloma (MM). The miR‐9 expression was examined by quantitative real‐time polymerase chain reaction assay. Transfection with miR‐9‐mimics, miR‐9‐inhibitor, pcDNA‐TRIM56, or si‐TRIM56 into cells was used to change the expression levels of miR‐9 and TRIM56. Western blot analysis was used to detect the expression of TRIM56, p65, p‐p65, IκBα, and p‐IκBα. The potential target of miR‐9 was confirmed by luciferase reporter assay. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium (MTT) assay, colony formation assay, and flow cytometry were used to assess the abilities of cell proliferation and apoptosis. miR‐9 was upregulated in MM patients and cell lines, and miR‐9 overexpression promoted proliferation and repressed apoptosis in MM cell lines. TRIM56 was confirmed as a target of miR‐9. Moreover, TRIM56 reversed miR‐9‐mediated pro‐proliferation and anti‐apoptosis effect on MM cell lines. Furthermore, nuclear factor‐κB (NF‐κB) pathway was involved in miR‐9/TRIM56‐mediated regulation on MM cell lines. miR‐9 promoted the development and progression of MM by regulating TRIM56/NF‐κB pathway, thereby providing a potential microRNA‐based target for MM therapy.
    Type of Medium: Online Resource
    ISSN: 1065-6995 , 1095-8355
    URL: Issue
    URL: Article
    DOI: 10.1002/cbin.v43.11
    DOI: 10.1002/cbin.11104
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1462519-2
    SSG: 12
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  • 7
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    Establishment of a new protein C detection system based on chromogenic substrate assay and its clinical diagnostic value for deep vein thrombosis
    Wiley ; 2021
    In:  Journal of Clinical Laboratory Analysis Vol. 35, No. 12 ( 2021-12)
    Details
    In: Journal of Clinical Laboratory Analysis, Wiley, Vol. 35, No. 12 ( 2021-12)
    Abstract: Deficiency of protein C (PC) affects the balance between blood coagulation and fibrinolysis in the human body. Chromogenic‐based assay is recommended as the preferred screening method for detecting PC deficiency. We established a PC detection system based on the chromogenic substrate assay. Methods First, a kit for the determination of PC activity in plasma was elaborately developed and its reaction parameters on XL‐3200c were explored. Then, we evaluated its performance and collected specimens to compare the test results obtained with those of the Siemens detection system. Finally, the clinical diagnostic efficacy of this detection system for deep vein thrombosis (DVT) was assessed. Results Optimum conditions for PC detection were 0.25–0.1 U/ml protein C activator Protac ® and 2.5–1 mM Pefachrome ® PCa5297. The composition and concentration ranges of buffer substances and stabilizers in the kit were also explored. Satisfactory results were observed in performance evaluation. The test results of the newly built detection system were highly correlated with those of the Siemens detection system ( R 2  = 0.9771 in the control group and R 2  = 0.9776 in the DVT group), and Bland‐Altman plots also showed high consistency between the two detection systems. In addition, the area under the curve (AUC) of the newly built PC detection system for DVT was 0.888, indicating this system could effectively improve the diagnostic sensitivity and specificity for DVT. Conclusion In this study, a sensitive, wide linear range and reliable PC activity detection system were established.
    Type of Medium: Online Resource
    ISSN: 0887-8013 , 1098-2825
    URL: Issue
    URL: Article
    DOI: 10.1002/jcla.v35.12
    DOI: 10.1002/jcla.24109
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2001635-9
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  • 8
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    Indium oxide nanoparticles induce lung intercellular toxicity between bronchial epithelial cells and macrophages
    Wiley ; 2020
    In:  Journal of Applied Toxicology Vol. 40, No. 12 ( 2020-12), p. 1636-1646
    Details
    In: Journal of Applied Toxicology, Wiley, Vol. 40, No. 12 ( 2020-12), p. 1636-1646
    Abstract: The effects of indium oxide nanoparticles (IO‐NPs) on lung cells associated with respiratory and immune barriers and the toxic effects of intercellular cascades were studied. We found IO‐NPs could directly damage pulmonary epithelial cells. Indium ions released by epithelial cells affect the phagocytosis and migration of macrophages, which may be a new point for the decrease in the clearance of alveolar surfactants and the development of IO‐related pulmonary alveolar proteinosis.
    Type of Medium: Online Resource
    ISSN: 0260-437X , 1099-1263
    URL: Issue
    URL: Article
    DOI: 10.1002/jat.v40.12
    DOI: 10.1002/jat.4023
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 1475015-6
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  • 9
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    A spatial and cellular distribution of rabies virus infection in the mouse brain revealed by fMOST and single‐cell RNA sequencing
    Wiley ; 2022
    In:  Clinical and Translational Medicine Vol. 12, No. 1 ( 2022-01)
    Details
    In: Clinical and Translational Medicine, Wiley, Vol. 12, No. 1 ( 2022-01)
    Abstract: Neurotropic virus infection can cause serious damage to the central nervous system (CNS) in both humans and animals. The complexity of the CNS poses unique challenges to investigate the infection of these viruses in the brain using traditional techniques. Methods In this study, we explore the use of fluorescence micro‐optical sectioning tomography (fMOST) and single‐cell RNA sequencing (scRNA‐seq) to map the spatial and cellular distribution of a representative neurotropic virus, rabies virus (RABV), in the whole brain. Mice were inoculated with a lethal dose of a recombinant RABV encoding enhanced green fluorescent protein (EGFP) under different infection routes, and a three‐dimensional (3D) view of RABV distribution in the whole mouse brain was obtained using fMOST. Meanwhile, we pinpointed the cellular distribution of RABV by utilizing scRNA‐seq. Results Our fMOST data provided the 3D view of a neurotropic virus in the whole mouse brain, which indicated that the spatial distribution of RABV in the brain was influenced by the infection route. Interestingly, we provided evidence that RABV could infect multiple nuclei related to fear independent of different infection routes. More surprisingly, our scRNA‐seq data revealed that besides neurons RABV could infect macrophages and the infiltrating macrophages played at least three different antiviral roles during RABV infection. Conclusion This study draws a comprehensively spatial and cellular map of typical neurotropic virus infection in the mouse brain, providing a novel and insightful strategy to investigate the pathogenesis of RABV and other neurotropic viruses.
    Type of Medium: Online Resource
    ISSN: 2001-1326 , 2001-1326
    URL: Issue
    URL: Article
    DOI: 10.1002/ctm2.v12.1
    DOI: 10.1002/ctm2.700
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2697013-2
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  • 10
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    Detection of HCV genotypes 1b and 2a by a reverse transcription loop‐mediated isothermal amplification assay
    Wiley ; 2017
    In:  Journal of Medical Virology Vol. 89, No. 6 ( 2017-06), p. 1048-1054
    Details
    In: Journal of Medical Virology, Wiley, Vol. 89, No. 6 ( 2017-06), p. 1048-1054
    Abstract: Hepatitis C virus (HCV) genotypes 1b and 2a are the major cause of liver disease in northern China; however, conventional detection tools are labor‐consuming, technically demanding, and costly. Here, we assessed the specificity, sensitivity, and clinical utility of reverse transcription loop‐mediated isothermal amplification (RT‐LAMP) assay for detection of HCV genotypes 1b and 2a. Firstly, clinical samples were collected from HCV genotype 1b and 2a infected patients and the RNA were extracted. Secondly, specificity of RT‐LAMP assay for detection HCV genotypes 1b and 2a were tested against viral genomes of other hepatitis viruses. Sensitivity of RT‐LAMP assay was determined using serial dilutions of standard HCV genotypes 1b and 2a. The amplified products were detected by both electrophoresis and calcein/Mn 2+ ‐dependent visual methods. Finally, we compared the clinical detection rate of RT‐LAMP to that of real‐time PCR. RT‐LAMP assay showed high specificity to detect HCV genotypes 1b and 2b since there was no cross‐reactivity with other hepatitis viruses. Sensitivity of RT‐LAMP was 100 IU/mL for both genotypes detected by either electrophoresis or calcein/Mn 2+ ‐dependent visual methods. The detection rate of RT‐LAMP assay in clinical samples was also comparable to that of real‐time PCR without significant difference between the both assays. This study proposes a newly developed RT‐LAMP assay for detection of HCV genotypes 1b and 2a. RT‐LAMP is highly specific, sensitive, and simple diagnostic tool which would be useful for screening and early diagnosis of HCV especially in resource‐limited environments.
    Type of Medium: Online Resource
    ISSN: 0146-6615 , 1096-9071
    URL: Issue
    URL: Article
    DOI: 10.1002/jmv.v89.6
    DOI: 10.1002/jmv.24747
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 752392-0
    detail.hit.zdb_id: 1475090-9
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