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  • 1
    Online Resource
    Online Resource
    Safety and benefit of modified mesohepatectomy in the treatment of bilobar involvement congenital biliary dilatation
    Wiley ; 2024
    In:  World Journal of Surgery
    Details
    In: World Journal of Surgery, Wiley
    Abstract: The diseased bile duct in bilobar congenital biliary dilatation is extensive and often requires major hepatectomy or liver transplantation associated with a higher risk. We aimed to evaluate the safety and benefit of modified mesohepatectomy, in comparison with trisectionectomy, to treat bilobar congenital biliary dilatation. Methods This study included 28 patients with type IV and V bilobar congenital biliary dilatation. An innovative mesohepatectomy comprising the hepatectomy technique beyond the P/U point and bile duct shaping was applied to 14 patients to address the extensively diseased bile duct and difficulty in hepaticojejunostomy. Another 14 patients received trisectionectomy. The perioperative and long‐term outcomes of these patients were compared. Results The ratio of residual liver volume to standard liver volume in the mesohepatectomy group was higher (78.68% vs. 40.90%, p  = 0.005), while the resection rate of the liver parenchyma was lower (28.25% vs. 63.97%, p  = 0.000), than that in trisectionectomy group. The mesohepatectomy group had a lower severe complication ( 〉 Clavein III, 0% vs. 57.70%, p  = 0.019) and incidence of posthepatectomy liver failure (7.14% vs. 42.86%, p  = 0.038). No significant difference was observed in blood loss and bile leakage ( p   〉  0.05). All the patients in the mesohepatectomy group achieved optimal results in the long‐term follow‐up. Conclusions mesohepatectomy provides an efficient treatment option for bilobar congenital biliary dilatation and can achieve radical resection, retain more liver parenchyma, and reduce the difficulty of hepaticojejunostomy, especially for patients that are not eligible for major hepatectomy and liver transplantation.
    Type of Medium: Online Resource
    ISSN: 0364-2313 , 1432-2323
    URL: Article
    DOI: 10.1002/wjs.12036
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 1463296-2
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  • 2
    Online Resource
    Online Resource
    High‐risk lymph node ratio predicts worse prognosis in patients with locally advanced oral cancer
    Wiley ; 2020
    In:  Journal of Oral Pathology & Medicine Vol. 49, No. 8 ( 2020-09), p. 787-795
    Details
    In: Journal of Oral Pathology & Medicine, Wiley, Vol. 49, No. 8 ( 2020-09), p. 787-795
    Abstract: To investigate the prognostic value of lymph node ratio (LNR), as well as the correlation with docetaxel, cisplatin, and 5‐FU (TPF) induction chemotherapy, in patients with locally advanced oral squamous cell carcinoma (OSCC). Methods Two‐hundred and forty‐five patients from a phase 3 trial involving TPF induction chemotherapy in stage III/IVA OSCC patients (NCT01542931) were enrolled in this study between 2008 and 2010. The clinical and pathological data were collected and analyzed. The cutoff value for LNR was calculated on the receiver operating characteristic (ROC) curve. Univariate and multivariate Cox regression models, and Kaplan‐Meier method were used for survival analysis. Results According to the ROC curve, the cutoff value for LNR was 7.6%. With a median follow‐up period of 80 months, the OSCC patients with high‐risk LNR ( 〉 7.6%), or positive extranodal extension (ENE) had significantly worse clinical outcomes than patients with low‐risk LNR (≤7.6%) or negative ENE. Multivariate analysis on pathological covariates showed that only high‐risk LNR was an independent negative predictive factor for survival ( P   〈  .05). The cutoff value of LNR of 7.6% was also verified with the similar results using an open TCGA database, high‐risk LNR indicating worse overall survival ( P   〈  .001) and disease‐free survival ( P   〈  .001). Conclusion Oral squamous cell carcinoma patients with high‐risk LNR have a worse clinical outcome than patients with low‐risk LNR. High‐risk LNR is an independent negative predictive factor for clinical outcome in patients with locally advanced OSCC.
    Type of Medium: Online Resource
    ISSN: 0904-2512 , 1600-0714
    URL: Issue
    URL: Article
    DOI: 10.1111/jop.v49.8
    DOI: 10.1111/jop.13043
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2026385-5
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  • 3
    Online Resource
    Online Resource
    Stathmin guides personalized therapy in oral squamous cell carcinoma
    Wiley ; 2020
    In:  Cancer Science Vol. 111, No. 4 ( 2020-04), p. 1303-1313
    Details
    In: Cancer Science, Wiley, Vol. 111, No. 4 ( 2020-04), p. 1303-1313
    Abstract: The survival benefit from docetaxel, cisplatin and 5‐fluorouracil (TPF) induction chemotherapy in oral squamous cell carcinoma (OSCC) patients is not satisfactory. Previously, we identified that stathmin, a microtubule‐destabilizing protein, is overexpressed in OSCC. Here, we further investigated its role as a biomarker that impacts on OSCC chemosensitivity. We analyzed the predictive value of stathmin on TPF induction chemotherapy and its impact on OSCC cell chemosensitivity. Then, we further investigated the therapeutic effects of the combination therapy of TPF chemotherapy and PI3K‐AKT‐mTOR inhibitors in vitro and in vivo. We found that OSCC patients with low stathmin expression benefited from TPF induction chemotherapy, while OSCC patients with high stathmin expression could not benefit from TPF induction chemotherapy. Stathmin overexpression promoted cellular proliferation and decreased OSCC cell sensitivity to TPF treatment. In addition, inhibition of the PI3K‐AKT‐mTOR signaling pathway decreased stathmin expression and phosphorylation. The combination therapy of TPF chemotherapy and PI3K‐AKT‐mTOR inhibitors exhibited a potent antitumor effect both in vitro and in vivo. Therefore, stathmin can be used as a predictive biomarker for TPF induction chemotherapy and a combination therapy regimen based on stathmin expression might improve the survival of OSCC patients.
    Type of Medium: Online Resource
    ISSN: 1347-9032 , 1349-7006
    URL: Issue
    URL: Article
    DOI: 10.1111/cas.v111.4
    DOI: 10.1111/cas.14323
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2115647-5
    detail.hit.zdb_id: 2111204-6
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  • 4
    Online Resource
    Online Resource
    Electrochemical Single‐Cell Protein Therapeutics Using a Double‐Barrel Nanopipette
    Wiley ; 2023
    In:  Angewandte Chemie Vol. 135, No. 9 ( 2023-02-20)
    Details
    In: Angewandte Chemie, Wiley, Vol. 135, No. 9 ( 2023-02-20)
    Abstract: Single‐cell protein therapeutics is expected to promote our in‐depth understanding of how a specific protein with a therapeutic dosage treats the cell without population averaging. However, it has not yet been tackled by current single‐cell nanotools. We address this challenge by the use of a double‐barrel nanopipette, in which one lumen was used for electroosmotic cytosolic protein delivery and the other was customized for ionic evaluation of the consequence. Upon injection of protein DJ‐1 through the delivery lumen, upregulation of the antioxidant protein could protect neural PC‐12 cells against oxidative stress from phorbol myristate acetate exposure, as deduced by targeting of the cytosolic hydrogen peroxide by the detecting lumen. The nanotool developed in this study for single‐cell protein therapeutics provides a perspective for future single‐cell therapeutics involving different therapeutic modalities, such as peptides, enzymes and nucleic acids.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v135.9
    DOI: 10.1002/ange.202215801
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
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  • 5
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    Online Resource
    An Integrated Dual‐Functional Nanotool Capable of Studying Single‐Cell Epigenetics and Programmable Gene Regulation
    Wiley ; 2023
    In:  Angewandte Chemie Vol. 135, No. 29 ( 2023-07-17)
    Details
    In: Angewandte Chemie, Wiley, Vol. 135, No. 29 ( 2023-07-17)
    Abstract: Single‐cell epigenetics is envisioned to decipher manifold epigenetic phenomena and to contribute to our accurate knowledge about basic epigenetic mechanisms. Engineered nanopipette technology has gained momentum in single‐cell studies; however, solutions to epigenetic questions remain unachieved. This study addresses the challenge by exploring N6‐methyladenine (m 6 A)‐bearing deoxyribozyme (DNAzyme) confined within a nanopipette for profiling a representative m 6 A‐modifying enzyme, fat mass and obesity‐associated protein (FTO). Electroosmotic intracellular extraction of FTO could remove the m 6 A and cause DNAzyme cleavage, leading to the altered ionic current signal. Because the cleavage can release a DNA sequence, we simultaneously program it as an antisense strand against FTO‐mRNA, intracellular injection of which has been shown to induce early stage apoptosis. This nanotool thus features the dual functions of studying single‐cell epigenetics and programmable gene regulation.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v135.29
    DOI: 10.1002/ange.202302930
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
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  • 6
    Online Resource
    Online Resource
    Outcomes of endoscopic and microscopic transsphenoidal surgery on non‐functioning pituitary adenomas: a systematic review and meta‐analysis
    Wiley ; 2018
    In:  Journal of Cellular and Molecular Medicine Vol. 22, No. 3 ( 2018-03), p. 2023-2027
    Details
    In: Journal of Cellular and Molecular Medicine, Wiley, Vol. 22, No. 3 ( 2018-03), p. 2023-2027
    Abstract: Both microscopic and endoscopic transsphenoidal surgery are effective approaches for nonfunctioning pituitary adenomas. The issue on the comparison of their efficacy and safety remains inconsistent. A thorough search of the literatures (PubMed, EMBASE , MEDLINE ) were performed up to March 2017. Studies reporting outcomes of microscopic or endoscopic transsphenoidal surgery on nonfunctioning pituitary adenomas were included. A meta‐analysis was performed focusing on the early stage and long term outcomes. The final search yielded 19 eligible studies enrolling 3847 patients, 389 of them underwent microscopic approach and 3458 of them with endoscopic approach. As to the early stage outcomes, the rate of gross tumor resection was significantly higher in the endoscopic group than that in microscopic group (73% versus 60%, P 〈 0.001). Meanwhile, endoscopic approach showed priority over microscopy on postoperative hypopituitarism (63% versus 65%, P 〈 0.001) and CSF leakage (3% versus 7%, P 〈 0.001). For the long term outcomes, the rate of visual improvement was significant higher in the endoscopic group than that in microscopic group (77% versus 50%, P 〈 0.001). However, there was no significant difference between the groups regarding the rate of permanent diabetic insipidus and meningitis. The endoscopic approach may be associated with higher rate of gross tumor movement and lower risk of postoperatively complications for treating nonfunctioning pituitary adenoma, when compared with microscopic approach. However, the confidence was shorted due to limited high quality evidence (largely randomized and controlled studies).
    Type of Medium: Online Resource
    ISSN: 1582-1838 , 1582-4934
    URL: Issue
    URL: Article
    DOI: 10.1111/jcmm.2018.22.issue-3
    DOI: 10.1111/jcmm.13445
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2076114-4
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  • 7
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    Online Resource
    An Integrated Photoelectrochemical Nanotool for Intracellular Drug Delivery and Evaluation of Treatment Effect
    Wiley ; 2021
    In:  Angewandte Chemie Vol. 133, No. 49 ( 2021-12), p. 25966-25969
    Details
    In: Angewandte Chemie, Wiley, Vol. 133, No. 49 ( 2021-12), p. 25966-25969
    Abstract: With reduced background and high sensitivity, photoelectrochemistry (PEC) may be applied as an intracellular nanotool and open a new technological direction of single‐cell study. Nevertheless, the present palette of single‐cell tools lacks such a PEC‐oriented solution. Here a dual‐functional photocathodic single‐cell nanotool capable of direct electroosmotic intracellular drug delivery and evaluation of oxidative stress is devised by engineering a target‐specific organic molecule/NiO/Ni film at the tip of a nanopipette. Specifically, the organic molecule probe serves simultaneously as the biorecognition element and sensitizer to synergize with p‐type NiO. Upon intracellular delivery at picoliter level, the oxidative stress effect will cause structural change of the organic probe, switching its optical absorption and altering the cathodic response. This work has revealed the potential of PEC single‐cell nanotool and extended the boundary of current single‐cell electroanalysis.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v133.49
    DOI: 10.1002/ange.202111608
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
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  • 8
    Online Resource
    Online Resource
    Electrochemical Single‐Cell Protein Therapeutics Using a Double‐Barrel Nanopipette
    Wiley ; 2023
    In:  Angewandte Chemie International Edition Vol. 62, No. 9 ( 2023-02-20)
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 62, No. 9 ( 2023-02-20)
    Abstract: Single‐cell protein therapeutics is expected to promote our in‐depth understanding of how a specific protein with a therapeutic dosage treats the cell without population averaging. However, it has not yet been tackled by current single‐cell nanotools. We address this challenge by the use of a double‐barrel nanopipette, in which one lumen was used for electroosmotic cytosolic protein delivery and the other was customized for ionic evaluation of the consequence. Upon injection of protein DJ‐1 through the delivery lumen, upregulation of the antioxidant protein could protect neural PC‐12 cells against oxidative stress from phorbol myristate acetate exposure, as deduced by targeting of the cytosolic hydrogen peroxide by the detecting lumen. The nanotool developed in this study for single‐cell protein therapeutics provides a perspective for future single‐cell therapeutics involving different therapeutic modalities, such as peptides, enzymes and nucleic acids.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v62.9
    DOI: 10.1002/anie.202215801
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
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  • 9
    Online Resource
    Online Resource
    A Photoelectrochemical Nanoreactor for Single‐Cell Sampling and Near Zero‐Background Faradaic Detection of Intracellular microRNA
    Wiley ; 2022
    In:  Angewandte Chemie Vol. 134, No. 47 ( 2022-11-21)
    Details
    In: Angewandte Chemie, Wiley, Vol. 134, No. 47 ( 2022-11-21)
    Abstract: Rational utilization of the rich light‐bio‐matter interplay taking place in single‐cell analysis represents a new technological direction in the field. The light‐fueled operation is expected to achieve advanced photoelectrochemical (PEC) single‐cell analysis with unknown possibilities. Here, a PEC nanoreactor capable of single‐cell sampling and near zero‐background Faradaic detection of intracellular microRNA (miR) is devised by the construction of a small reaction chamber accommodating the target‐triggered hybridization chain reaction for binding the metallointercalator of [Ru(bpy) 2 (dppz)] 2+ as the signal reporter. Light stimulation of the dsDNA/metallointercalator adduct will induce the generation of photocurrents, underpinning a zero‐biased and near zero‐background PEC method toward Faradaic detection of non‐electrogenic miR at the single‐cell level. Using this nanotool, lower miR concentration in the near‐nucleus region than that in the main cytosol was revealed.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v134.47
    DOI: 10.1002/ange.202212752
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
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  • 10
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    Online Resource
    Functional nucleic acid engineered double‐barreled nanopores for measuring sodium to potassium ratio at single‐cell level
    Wiley ; 2022
    In:  Exploration Vol. 2, No. 5 ( 2022-10)
    Details
    In: Exploration, Wiley, Vol. 2, No. 5 ( 2022-10)
    Abstract: The use of double‐barreled nanopipette (θ‐nanopipette) to electrically sample, manipulate, or detect biomaterials has recently seen strong growth in single‐cell studies, driven by the potential of the nanodevices and applications that they may enable. Considering the pivotal roles of Na/K ratio (R Na/K ) at cellular level, herein we describe an engineered θ‐nanopipette for measuring single‐cell R Na/K . The two independently addressable nanopores, located within one nanotip, allow respective customization of functional nucleic acids but simultaneous deciphering of Na and K levels inside a single cell of a non‐Faradic manner. Two ionic current rectification signals, corresponding to the Na‐ and K‐specific smart DNA responses, could be easily used to derive the R Na/K . The applicability of this nanotool is validated by practical probing intracellular R Na/K during the drug‐induced primary stage of apoptotic volume decrease. Especially, the R Na/K has been shown by our nanotool to be different in cell lines with different metastatic potential. This work is expected to contribute to futuristic study of single‐cell R Na/K in various physiological and pathological processes.
    Type of Medium: Online Resource
    ISSN: 2766-2098 , 2766-2098
    URL: Issue
    URL: Article
    DOI: 10.1002/exp2.v2.5
    DOI: 10.1002/EXP.20220025
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 3103468-8
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