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  • 1
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    Degree‐based statistic and center persistency for brain connectivity analysis
    Wiley ; 2017
    In:  Human Brain Mapping Vol. 38, No. 1 ( 2017-01), p. 165-181
    Details
    In: Human Brain Mapping, Wiley, Vol. 38, No. 1 ( 2017-01), p. 165-181
    Abstract: Brain connectivity analyses have been widely performed to investigate the organization and functioning of the brain, or to observe changes in neurological or psychiatric conditions. However, connectivity analysis inevitably introduces the problem of mass‐univariate hypothesis testing. Although, several cluster‐wise correction methods have been suggested to address this problem and shown to provide high sensitivity, these approaches fundamentally have two drawbacks: the lack of spatial specificity (localization power) and the arbitrariness of an initial cluster‐forming threshold. In this study, we propose a novel method, degree‐based statistic (DBS), performing cluster‐wise inference. DBS is designed to overcome the above‐mentioned two shortcomings. From a network perspective, a few brain regions are of critical importance and considered to play pivotal roles in network integration. Regarding this notion, DBS defines a cluster as a set of edges of which one ending node is shared. This definition enables the efficient detection of clusters and their center nodes. Furthermore, a new measure of a cluster, center persistency (CP) was introduced. The efficiency of DBS with a known “ground truth” simulation was demonstrated. Then they applied DBS to two experimental datasets and showed that DBS successfully detects the persistent clusters. In conclusion, by adopting a graph theoretical concept of degrees and borrowing the concept of persistence from algebraic topology, DBS could sensitively identify clusters with centric nodes that would play pivotal roles in an effect of interest. DBS is potentially widely applicable to variable cognitive or clinical situations and allows us to obtain statistically reliable and easily interpretable results. Hum Brain Mapp 38:165–181, 2017 . © 2016 Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 1065-9471 , 1097-0193
    URL: Issue
    URL: Article
    DOI: 10.1002/hbm.v38.1
    DOI: 10.1002/hbm.23352
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 1492703-2
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  • 2
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    Alpha‐synuclein in gastric and colonic mucosa in Parkinson's disease: Limited role as a biomarker
    Wiley ; 2016
    In:  Movement Disorders Vol. 31, No. 2 ( 2016-02), p. 241-249
    Details
    In: Movement Disorders, Wiley, Vol. 31, No. 2 ( 2016-02), p. 241-249
    Abstract: Gastric and colonic alpha‐synuclein immunoreactivity has been reported in patients with Parkinson's disease (PD). However, enteric alpha‐synuclein also has been reported in healthy individuals. Objectives We aimed to investigate the utility of alpha‐synuclein immunoreactivity from gastric and colonic mucosal tissues obtained by routine endoscopy to detect PD, and to correlate the pathological burden of alpha‐synuclein with motor and nonmotor features of PD. Methods We recruited 104 study subjects, consisting of 38 patients with PD, 13 patients with probable multiple system atrophy (MSA), and 53 healthy controls. Gastric and colonic mucosal tissues obtained by endoscopic gastroduodenoscopy and colonoscopy were assessed using alpha‐synuclein immunohistochemistry. Detailed motor and nonmotor features of PD were correlated with enteric alpha‐synuclein immunoreactivity. Results No difference was seen in the enteric α‐SYN immunoreactivity among patients with PD (31.6% for stomach and 10.4% for colon), patients with MSA (40.0% for stomach and 8.0% for colon), and healthy controls (33.3% for stomach and 18.5% for colon). The frequency of positive alpha‐synuclein immunoreactivity was higher in gastric biopsy tissues than in colonic biopsy tissues in all of the study groups ( P   〈  0.05). No significant correlation was found between the presence of alpha‐synuclein immunoreactivity and the motor and nonmotor features of PD. Conclusions The presence of alpha‐synuclein immunoreactivity in gastric and colonic mucosa was detected in a similar manner in patients with PD, patients with MSA, and controls, thus suggesting a limited role of enteric mucosal alpha‐synuclein as a diagnostic biomarker for PD. Future studies are warranted to detect pathological alpha‐synuclein strains. © 2015 International Parkinson and Movement Disorder Society
    Type of Medium: Online Resource
    ISSN: 0885-3185 , 1531-8257
    URL: Issue
    URL: Article
    DOI: 10.1002/mds.v31.2
    DOI: 10.1002/mds.26473
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2041249-6
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  • 3
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    Dysport and Botox at a ratio of 2.5:1 units in cervical dystonia: A double‐blind, randomized study
    Wiley ; 2015
    In:  Movement Disorders Vol. 30, No. 2 ( 2015-02), p. 206-213
    Details
    In: Movement Disorders, Wiley, Vol. 30, No. 2 ( 2015-02), p. 206-213
    Abstract: We aimed to compare Dysport (abobotulinumtoxinA, Ipsen Biopharm, Slough, UK) and Botox (onabotulinumtoxinA, Allergan, Irvine, CA, USA) at a 2.5:1 ratio in the treatment of cervical dystonia (CD). A Dysport/Botox ratio of lower than 3:1 was suggested as a more appropriate conversion ratio, considering its higher efficacy and more frequent incidence of adverse effects not only in the treatment of CD but also in other focal movement disorders. A randomized, double‐blind, multicenter, non‐inferiority, two‐period crossover study was done in CD, with a duration of at least 18 months. Patients were randomly assigned to treatment for the first period with Dysport or Botox, and they were followed up for 16 weeks after the injection. After a 4‐week washout period, they were switched to the other formulation and then followed up for 16 weeks. The primary outcome was the changes in the Tsui scale between the baseline value and that at 1 month after each injection. A total of 103 patients were enrolled, and 94 completed the study. Mean changes in the Tsui scale between baseline and 4 weeks after each injection tended to favor Botox; however, this was not statistically significant (4.0 ± 3.9 points for the Dysport treatment vs. 4.8 ± 4.1 points for Botox; 95% confidence interval, −0.1‐1.7; P  = 0.091). The mean change of the Toronto western spasmodic torticollis rating scale score, the proportion of improvement in clinical global impression and patient global impression, and the incidences of adverse events were not significantly different between the two treatments. With regard to safety and efficacy, Dysport was not inferior to Botox in patients with CD at a conversion factor of 2.5:1. [ clinicaltrial.gov : NCT00950664] © The Authors. Movement Disorders published by Wiley Periodicals, Inc. on behalf of International Parkinson and Movement Disorder Society.
    Type of Medium: Online Resource
    ISSN: 0885-3185 , 1531-8257
    URL: Issue
    URL: Article
    DOI: 10.1002/mds.v30.2
    DOI: 10.1002/mds.26085
    RVK:
    XA 10000
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2041249-6
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  • 4
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    A multiresidue method for the analysis of pesticide residues in polished rice (Oryza sativa L.) using accelerated solvent extraction and gas chromatography and confirmation by mass spectrometry
    Wiley ; 2007
    In:  Biomedical Chromatography Vol. 21, No. 6 ( 2007-06), p. 602-609
    Details
    In: Biomedical Chromatography, Wiley, Vol. 21, No. 6 ( 2007-06), p. 602-609
    Type of Medium: Online Resource
    ISSN: 0269-3879 , 1099-0801
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.1002/bmc.v21:6
    DOI: 10.1002/(ISSN)1099-0801
    DOI: 10.1002/bmc.792
    Language: English
    Publisher: Wiley
    Publication Date: 2007
    detail.hit.zdb_id: 1479945-5
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  • 5
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    Simultaneous determination of three acidic herbicide residues in food crops using HPLC and confirmation via LC‐MS/MS
    Wiley ; 2011
    In:  Biomedical Chromatography Vol. 25, No. 1-2 ( 2011-01), p. 124-135
    Details
    In: Biomedical Chromatography, Wiley, Vol. 25, No. 1-2 ( 2011-01), p. 124-135
    Abstract: 2,4‐D, dicamba and 4‐CPA with auxin‐like activity have been intensively used in agriculture, for the control of unwanted broadleaf weeds. An analytical method involving HPLC coupled with UVD was developed for the simultaneous analysis of these three analytes in Chinese cabbage, apple and pepper fruits (representative non‐fatty samples) and brown rice and soybean (representative fatty samples) using liquid‐liquid partitioning and column cleanup procedures. The residues were confirmed via tandem mass spectrometry (MS/MS) in ion electrospray ionization (ESI) mode. The standard curves were linear over the range of the tested concentrations (0.25–10 µg/mL), as shown by a marked linearity in excess of 0.9999 ( r 2 ). The average recoveries (mean, n = 3) ranged from 94.30 to 102.63 in Chinese cabbage, from 94.76 to 108.47 in apple, from 97.52 to 102.27 in pepper, from 76.19 to 101.90 in brown rice, and from 74.60 to 107.39 in soybean. The relative standard deviations (RSDs) were 〈 9% in all tested matrices. The limits of detection and quantitation were 0.006 and 0.02 mg/kg, respectively. Samples purchased from local markets were analyzed to evaluate the applicability of the methods developed herein. The concentration of the 2,4‐D residue was measured at 0.102 mg/kg in the soybean sample; however, this level is exactly the same MRL set by the Korea Food and Drug Administration. This developed method deserves full and complete consideration, as it clearly displays the sensitivity, accuracy and precision required for residue analysis of 2,4‐D, dicamba and 4‐CPA in food crops. Copyright © 2010 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 0269-3879 , 1099-0801
    URL: Issue
    URL: Article
    DOI: 10.1002/bmc.v25.1/2
    DOI: 10.1002/bmc.1513
    Language: English
    Publisher: Wiley
    Publication Date: 2011
    detail.hit.zdb_id: 1479945-5
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  • 6
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    Dominant Negative N‐Cadherin Inhibits Osteoclast Differentiation by Interfering With β‐Catenin Regulation of RANKL, Independent of Cell‐Cell Adhesion
    Wiley ; 2005
    In:  Journal of Bone and Mineral Research Vol. 20, No. 12 ( 2005-12), p. 2200-2212
    Details
    In: Journal of Bone and Mineral Research, Wiley, Vol. 20, No. 12 ( 2005-12), p. 2200-2212
    Abstract: We studied the effects of dominant negative N‐cadherin (NCadΔC) expression in ST2 cells on their ability to support osteoclastogenesis. Expression of NCadΔC in ST2 cells did not decrease cell‐to‐cell adhesion but significantly reduced osteoclast formation when co‐cultured with BMMs. NCadΔC inhibited β‐catenin/TCF signaling, resulting in decreased RANKL expression, which could contribute to the reduced osteoclast formation. Introduction : Cadherin is a calcium‐dependent cell adhesion molecule that plays major roles during embryonic development and morphogenesis. Classic cadherins interact with β‐catenin, which is also involved in the Wnt signaling pathway. We tested whether disruption of N‐cadherin function in stromal cells by dominant negative N‐cadherin affects their ability to support osteoclastogenesis by altering heterotypic interaction with osteoclast precursors. Materials and Methods : ST2 cells were transduced with retrovirus encoding extracellular domain‐truncated, dominant negative N‐cadherin (NCadΔC) and co‐cultured with bone marrow macrophages (BMMs) to study the ability to support osteoclastogenesis. As a downstream target of NCadΔC, β‐catenin/T‐cell factor (TCF) transcriptional activity was analyzed using TOPflash reporter construct. Real‐time RT‐PCR analysis and RANKL‐luciferase reporter assays were performed to study the effects of NCadΔC on the osteoprotegerin (OPG)/RANKL system. Results : Immunoblotting analysis showed that primary bone marrow stromal cells, ST2 cells, and BMMs expressed N‐cadherin. Retroviral expression of NCadΔC in ST2 cells did not significantly inhibit cell adhesion but markedly impaired the formation of TRACP + osteoclasts ( 〉 40%) when co‐cultured with BMMs. However, the inhibition of osteoclastogenesis was not reproduced by neutralizing antibody against N‐cadherin. Expression of NCadΔC, however, strongly suppressed β‐catenin/TCF transcriptional activity in ST2 cells, which was rescued by constitutively active β‐catenin adenovirus (Ad ΔN46 β‐catenin) or constitutively active TCF mutant (pCS2‐VP16ΔβXTCF‐3). As a potential downstream target of Wnt signaling, we found that the expression of RANKL was reduced in ST2 cells expressing NCadΔC. Moreover, Wnt‐3A, Ad ΔN46 β‐catenin, and VP16ΔβXTCF‐3 increased the expression of RANKL and enhanced the transcriptional activity of mouse RANKL promoter in ST2 cells. Conclusions : Our data suggest that expression of dominant negative N‐cadherin in ST2 cells suppressed osteoclastogenesis by interfering with β‐catenin regulation of RANKL independent of cell‐cell adhesion.
    Type of Medium: Online Resource
    ISSN: 0884-0431 , 1523-4681
    URL: Article
    DOI: 10.1359/JBMR.050809
    RVK:
    XA 52230
    Language: English
    Publisher: Wiley
    Publication Date: 2005
    detail.hit.zdb_id: 2008867-X
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  • 7
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    A Comparison of the Incidence of Post‐Dural Puncture Headache and Backache After Spinal Anesthesia: A Pragmatic Randomized Controlled Trial
    Wiley ; 2018
    In:  Worldviews on Evidence-Based Nursing Vol. 15, No. 1 ( 2018-02), p. 45-53
    Details
    In: Worldviews on Evidence-Based Nursing, Wiley, Vol. 15, No. 1 ( 2018-02), p. 45-53
    Abstract: Although bed rest is recommended after spinal anesthesia to prevent the occurrence of post‐dural puncture headache, current literature suggests that periods of bed rest did not prevent headache as well as increase the risk of other complications such as backache. However, information is scarce regarding an appropriate period of bed rest following a dural puncture. Aim The aim of this study was to compare the incidence of post‐dural puncture headache and backache after different periods of bed rest following spinal anesthesia. Methods This study was a pragmatic, parallel‐group, blinded, and randomized controlled trial (trial registration number KCT0001797). A total of 119 patients who underwent surgery under spinal anesthesia participated in this study from December 2013 to June 2014. The participants were randomly allocated to three groups: the immediate mobilization group ( n = 45), 4‐hour bed rest group ( n = 40), and 6‐hour bed rest group ( n = 34). The severity of headache and backache was measured using the Dittmann scale and a visual analogue scale, respectively. Data were collected for 5 consecutive days postoperatively by one researcher blind to the group allocation. Results No significant difference in the incidence of headache among the three groups was detected. However, the incidence of backache in the 6‐hour bed rest group was higher and was significantly more severe than the other groups. Linking Evidence to Action Bed rest after spinal anesthesia did not prevent the occurrence of headache and increased the incidence of patients experiencing a backache and, therefore, is not recommended. The findings provide information for establishing evidence‐based nursing practices for patients after a dural puncture.
    Type of Medium: Online Resource
    ISSN: 1545-102X , 1741-6787
    URL: Issue
    URL: Article
    DOI: 10.1111/wvn.2018.15.issue-1
    DOI: 10.1111/wvn.12236
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2140041-6
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  • 8
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    Digital image analysis in pathologist‐selected regions of interest predicts survival more accurately than whole‐slide analysis: a direct comparison study in 153 gastric carcinomas
    Wiley ; 2021
    In:  The Journal of Pathology: Clinical Research Vol. 7, No. 1 ( 2021-01), p. 42-51
    Details
    In: The Journal of Pathology: Clinical Research, Wiley, Vol. 7, No. 1 ( 2021-01), p. 42-51
    Abstract: Automatic quantification of biomarkers such as tumor‐infiltrating lymphocytes and PD‐L1 is one of the most studied topics in digital pathology image analysis (DIA). However, direct comparison between the DIA of a whole‐slide image (WSI) and that of regions of interest (ROIs) chosen by pathologists has not been performed. In this study, we aimed to compare the prognostic value of tumor microenvironment markers CD8 and PD‐L1, measured by DIA of WSIs and ROIs. We selected 153 primary gastric cancer tissues and stained them with CD8 and PD‐L1. All IHC slides were scanned at ×200 magnification and ratios of CD8 and PD‐L1 were measured in WSIs and ROIs from the invasive front, within the tumor, and the mucosa. Patients with high CD8 and PD‐L1 ratios showed more favorable outcomes compared to those with low ratios. Pathologist‐aided DIA predicted the survival of patients more accurately than WSI analysis (CD8, p = 0.025 versus p = 0.068; PD‐L1, p = 0.008 versus p = 0.2). Although a high density of CD8+ T cells at the invasive front correlated best with patient survival, CD8 ratio in the mucosa could also predict patient outcome. In conclusion, CD8 and PD‐L1 ratios measured by pathologist‐aided DIA predicted survival more accurately than WSI analyses and ROIs at the invasive front correlated best with patient outcome.
    Type of Medium: Online Resource
    ISSN: 2056-4538 , 2056-4538
    URL: Issue
    URL: Article
    DOI: 10.1002/cjp2.v7.1
    DOI: 10.1002/cjp2.179
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2814357-7
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  • 9
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    Effects of Nicotine on Antioxidant Defense Enzymes and RANKL Expression in Human Periodontal Ligament Cells
    Wiley ; 2009
    In:  Journal of Periodontology Vol. 80, No. 8 ( 2009-08), p. 1281-1288
    Details
    In: Journal of Periodontology, Wiley, Vol. 80, No. 8 ( 2009-08), p. 1281-1288
    Abstract: Background: This study examined the effects of nicotine on osteoblastic differentiation and the osteoclastogenesis regulatory molecules receptor activator of nuclear factor‐kappa B ligand (RANKL) and osteoprotegerin (OPG). In addition, we investigated the mechanism by which nicotine induced antioxidant defense enzyme expression as a protective response. Methods: The expression of osteoblast markers, RANKL, OPG, and antioxidant defense enzymes were examined in nicotine‐treated human periodontal ligament (PDL) cells by reverse transcription‐polymerase chain reaction and Western blotting. Results: Nicotine treatment concomitantly downregulated the expression of OPG and osteoblastic differentiation markers, such as alkaline phosphatase, osteocalcin, and osteopontin, and upregulated the expression of RANKL. Nicotine induced the synthesis of the transcription factor NF‐E2‐related factor‐2 (Nrf2) as well as a number of cellular antioxidants and phase II enzymes, such as heme oxygenase‐1. Pretreatment with antioxidants inhibited the upregulation of RANKL, the downregulation of OPG expression, and cytotoxicity by nicotine in PDL cells. Conclusions: Nicotine upregulated RANKL and antioxidant defense enzymes. These data suggest that Nrf2‐mediated induction of cellular antioxidants and phase II enzymes could contribute to the cellular defense against nicotine‐induced cytotoxicity and osteoclastic differentiation in PDL cells.
    Type of Medium: Online Resource
    ISSN: 0022-3492 , 1943-3670
    URL: Article
    DOI: 10.1902/jop.2009.090098
    Language: English
    Publisher: Wiley
    Publication Date: 2009
    detail.hit.zdb_id: 2040047-0
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  • 10
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    High-risk human papillomavirus load and biomarkers in cervical intraepithelial neoplasia and cancer
    Wiley ; 2014
    In:  APMIS Vol. 122, No. 5 ( 2014-05), p. 427-436
    Details
    In: APMIS, Wiley, Vol. 122, No. 5 ( 2014-05), p. 427-436
    Type of Medium: Online Resource
    ISSN: 0903-4641
    URL: Issue
    URL: Article
    DOI: 10.1111/apm.2014.122.issue-5
    DOI: 10.1111/apm.12163
    RVK:
    XA 13160
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 2098213-6
    SSG: 12
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