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  • Wiley  (13)
  • 1
    Online Resource
    Online Resource
    In Situ Spatial Complementation of Aptamer‐Mediated Recognition Enables Live‐Cell Imaging of Native RNA Transcripts in Real Time
    Wiley ; 2018
    In:  Angewandte Chemie International Edition Vol. 57, No. 4 ( 2018-01-22), p. 972-976
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 57, No. 4 ( 2018-01-22), p. 972-976
    Abstract: Direct cellular imaging of the localization and dynamics of biomolecules helps to understand their function and reveals novel mechanisms at the single‐cell resolution. In contrast to routine fluorescent‐protein‐based protein imaging, technology for RNA imaging remains less well explored because of the lack of enabling technology. Herein, we report the development of an aptamer‐initiated fluorescence complementation (AiFC) method for RNA imaging by engineering a green fluorescence protein (GFP)‐mimicking turn‐on RNA aptamer, Broccoli, into two split fragments that could tandemly bind to target mRNA. When genetically encoded in cells, endogenous mRNA molecules recruited Split‐Broccoli and brought the two fragments into spatial proximity, which formed a fluorophore‐binding site in situ and turned on fluorescence. Significantly, we demonstrated the use of AiFC for high‐contrast and real‐time imaging of endogenous RNA molecules in living mammalian cells. We envision wide application and practical utility of this enabling technology to in vivo single‐cell visualization and mechanistic analysis of macromolecular interactions.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v57.4
    DOI: 10.1002/anie.201707795
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
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  • 2
    Online Resource
    Online Resource
    Preventing acute liver injury via hepatocyte‐targeting nano‐antioxidants
    Wiley ; 2023
    In:  Cell Proliferation
    Details
    In: Cell Proliferation, Wiley
    Abstract: Acute liver injury (ALI) is a severe liver disease that is characterized by sudden and massive hepatocyte necrosis and deterioration of liver functions. Oxidative stress is increasingly recognized as a key factor in the induction and progression of ALI. Scavenging excessive reactive oxygen species (ROS) with antioxidants has become a promising therapeutic option, but intrinsically hepatocyte‐targeting antioxidants with excellent bioavailability and biocompatibility are yet to be developed. Herein, self‐assembling nanoparticles (NPs) composed of amphiphilic polymers are introduced to encapsulate organic Selenium compound L‐Se‐methylselenocysteine (SeMC) and form SeMC NPs, which protect the viabilities and functions of cultured hepatocytes in drug‐ or chemical‐induced acute hepatotoxicity models via efficient ROS removal. After further functionalization with the hepatocyte‐targeting ligand glycyrrhetinic acid (GA), the resultant GA‐SeMC NPs exhibit enhanced hepatocyte uptake and liver accumulation. In mouse models of ALI induced by acetaminophen (APAP) or carbon tetrachloride (CCl 4 ), treatment with GA‐SeMC NPs significantly decrease the levels of hepatic lipid peroxidation, tissue vacuolization and serum liver transaminases, while prominently increase that of endogenous antioxidant enzymes. Our study therefore presents a liver‐targeting drug delivery strategy for the prevention and treatment of hepatic diseases.
    Type of Medium: Online Resource
    ISSN: 0960-7722 , 1365-2184
    URL: Article
    DOI: 10.1111/cpr.13494
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2019986-7
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    In Situ Spatial Complementation of Aptamer‐Mediated Recognition Enables Live‐Cell Imaging of Native RNA Transcripts in Real Time
    Wiley ; 2018
    In:  Angewandte Chemie Vol. 130, No. 4 ( 2018-01-22), p. 984-988
    Details
    In: Angewandte Chemie, Wiley, Vol. 130, No. 4 ( 2018-01-22), p. 984-988
    Abstract: Direct cellular imaging of the localization and dynamics of biomolecules helps to understand their function and reveals novel mechanisms at the single‐cell resolution. In contrast to routine fluorescent‐protein‐based protein imaging, technology for RNA imaging remains less well explored because of the lack of enabling technology. Herein, we report the development of an aptamer‐initiated fluorescence complementation (AiFC) method for RNA imaging by engineering a green fluorescence protein (GFP)‐mimicking turn‐on RNA aptamer, Broccoli, into two split fragments that could tandemly bind to target mRNA. When genetically encoded in cells, endogenous mRNA molecules recruited Split‐Broccoli and brought the two fragments into spatial proximity, which formed a fluorophore‐binding site in situ and turned on fluorescence. Significantly, we demonstrated the use of AiFC for high‐contrast and real‐time imaging of endogenous RNA molecules in living mammalian cells. We envision wide application and practical utility of this enabling technology to in vivo single‐cell visualization and mechanistic analysis of macromolecular interactions.
    Type of Medium: Online Resource
    ISSN: 0044-8249 , 1521-3757
    URL: Issue
    URL: Article
    DOI: 10.1002/ange.v130.4
    DOI: 10.1002/ange.201707795
    RVK:
    VA 2100
    RVK:
    VN 5020
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 505868-5
    detail.hit.zdb_id: 506609-8
    detail.hit.zdb_id: 514305-6
    detail.hit.zdb_id: 505872-7
    detail.hit.zdb_id: 1479266-7
    detail.hit.zdb_id: 505867-3
    detail.hit.zdb_id: 506259-7
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  • 4
    Online Resource
    Online Resource
    Enantioseparation of nine indanone and tetralone derivatives by HPLC using carboxymethyl‐β‐cyclodextrin as the mobile phase additive
    Wiley ; 2017
    In:  Chirality Vol. 29, No. 1 ( 2017-01), p. 38-47
    Details
    In: Chirality, Wiley, Vol. 29, No. 1 ( 2017-01), p. 38-47
    Abstract: High‐performance liquid chromatography (HPLC) is a powerful method in the area of chiral separation. In this study, a method of HPLC using carboxymethyl‐β‐cyclodextrin (CM‐β‐CD) as chiral selector was developed for enantioseparation of nine indanone and tetralone derivatives. The separation was performed on a conventional C 18 column. The optimal mobile phase was a mixture of methanol and 0.05 mol/L phosphate buffer at pH 1.8 (55:45, v /v) containing 22.9 mmol/L CM‐β‐CD. Under such conditions, the resolutions of all analytes were over 1.8 except for Compound F. The results of the study indicate the presence of a complex with 1:1 stoichiometry of the inclusion complex. In addition, it can be inferred from thermodynamic analysis that the behavior of formation of the inclusion complex and enantioseparation occurred simultaneously, while they were driven by different forces. The effect of analyte structure is also discussed.
    Type of Medium: Online Resource
    ISSN: 0899-0042 , 1520-636X
    URL: Issue
    URL: Article
    DOI: 10.1002/chir.v29.1
    DOI: 10.1002/chir.22665
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2001237-8
    SSG: 12
    SSG: 15,3
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  • 5
    Online Resource
    Online Resource
    Polymetallic Hybrid Nanoplatform with Hyperthermia‐Amplified Dual Enzyme‐Like Activities for Efficient Speeded‐Up Bacterially Infected Wound Healing
    Wiley ; 2022
    In:  Advanced Materials Interfaces Vol. 9, No. 31 ( 2022-11)
    Details
    In: Advanced Materials Interfaces, Wiley, Vol. 9, No. 31 ( 2022-11)
    Abstract: Antibiotic‐independent artificial nanozymes exhibit excellent biocatalytic activity, and can generate sufficient reactive oxygen species (ROS) for potential applications in the field of anti‐infective therapy. However, single nanozymes often catalyze the production of ROS with low yield and low utilization, resulting in low antibacterial activity. To address the above refractory concern, a spike‐like surface nanoparticles (CAC NPs) platform is built that possesses intrinsic dual enzyme‐like (oxidase and peroxidase‐like) activities and photothermal conversion capacity with near‐infrared irradiation (NIR‐I) to quickly capture and effectively eliminate the bacteria and biofilm. Upon NIR‐I irradiation, CAC NPs have exerted an excellent biocatalytic antibacterial effect through a hyperthermia‐amplified dual enzyme‐like activities strategy. As predicted, the results show CAC NPs can eradicate bacteria in the subcutaneous tissues of mice and promote the healing of inflamed tissues with no significant side effects in vivo. Collectively, this work reveals the potential of NIR‐assisted dual enzyme‐like activities for antimicrobial therapy and provides broad prospects for antimicrobial drugs and strategies.
    Type of Medium: Online Resource
    ISSN: 2196-7350 , 2196-7350
    URL: Issue
    URL: Article
    DOI: 10.1002/admi.v9.31
    DOI: 10.1002/admi.202201422
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2750376-8
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  • 6
    Online Resource
    Online Resource
    A Vehicle‐Free Antimicrobial Polymer Hybrid Gold Nanoparticle as Synergistically Therapeutic Platforms for Staphylococcus aureus Infected Wound Healing
    Wiley ; 2022
    In:  Advanced Science Vol. 9, No. 14 ( 2022-05)
    Details
    In: Advanced Science, Wiley, Vol. 9, No. 14 ( 2022-05)
    Abstract: Pathogenic bacteria infection is a serious threat to human public health due to the high morbidity and mortality rates. Nano delivery system for delivering antibiotics provides an alternative option to improve the efficiency compared to conventional therapeutic agents. In addition to the drug loading capacity of nanocarriers, which is typically around 10%, further lowers the drug dose that pathological bacteria are exposed to. Moreover, nanocarriers that are not eliminated from the body may cause side effects. These limitations have motivated the development of self‐delivery systems that are formed by the self‐assembly of different therapeutic agents. In this study, a vehicle‐free antimicrobial polymer polyhexamethylene biguanide (PHMB, with bactericidal and anti‐biofilm functions) hybrid gold nanoparticle (Au NPs, with photothermal therapy (PTT)) platform (PHMB@Au NPs) is developed. This platform exhibits an excellent synergistic effect to enhance the photothermal bactericidal effect for Staphylococcus aureus under near‐infrared irradiation. Furthermore, the results showed that PHMB@Au NPs inhibit the formation of biofilms, quickly remove bacteria to promote wound healing through PTT in infection model in vivo, and even mediate the transition of macrophages from M1 to M2 type, and accelerate tissue angiogenesis. PHMB@Au NPs will have promising value as highly effective antimicrobial agents for patient management.
    Type of Medium: Online Resource
    ISSN: 2198-3844 , 2198-3844
    URL: Issue
    URL: Article
    DOI: 10.1002/advs.v9.14
    DOI: 10.1002/advs.202105223
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2808093-2
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  • 7
    Online Resource
    Online Resource
    Polarity protein Canoe mediates overproliferation via modulation of JNK, Ras‐MAPK and Hippo signalling
    Wiley ; 2019
    In:  Cell Proliferation Vol. 52, No. 1 ( 2019-01)
    Details
    In: Cell Proliferation, Wiley, Vol. 52, No. 1 ( 2019-01)
    Abstract: Over the past decade an intriguing connection between cell polarity and tumorigenesis has emerged. Multiple core components of the junction complexes that help to form and maintain cell polarity display both pro‐ and anti‐tumorigenic functions in a context‐dependent manner, with the underlying mechanisms poorly understood. Materials and Methods With transgenic fly lines that overexpress or knock down specific signalling components, we perform genetic analysis to investigate the precise role of the polarity protein Canoe (Cno) in tumorigenesis and the downstream pathways. Results We show that overexpression of cno simultaneously activates JNK and Ras‐MEK‐ERK signalling, resulting in mixed phenotypes of both overproliferation and cell death in the Drosophila wing disc. Moderate alleviation of JNK activation eliminates the effect of Cno on cell death, leading to organ overgrowth and cell migration that mimic the formation and invasion of tumours. In addition, we find that the Hippo pathway acts downstream of JNK and Ras signalling to mediate the effect of Cno on cell proliferation. Conclusions Our work reveals an oncogenic role of Cno and creates a new type of Drosophila tumour model for cancer research.
    Type of Medium: Online Resource
    ISSN: 0960-7722 , 1365-2184
    URL: Issue
    URL: Article
    DOI: 10.1111/cpr.2019.52.issue-1
    DOI: 10.1111/cpr.12529
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2019986-7
    SSG: 12
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  • 8
    Online Resource
    Online Resource
    Programming Niche Accessibility and In Vitro Stemness with Intercellular DNA Reactions
    Wiley ; 2018
    In:  Advanced Materials Vol. 30, No. 46 ( 2018-11)
    Details
    In: Advanced Materials, Wiley, Vol. 30, No. 46 ( 2018-11)
    Abstract: Stem cells generally exist in low abundance and tend to lose stemness in the absence of self‐renewal signals. While extracellular‐matrix‐mimicking techniques have been developed to support stem cell proliferation, the lack of niche cells in these synthetic systems often hampers continuous stem cell expansion and maintenance of pluripotency, which are indispensable for regenerative medicine. Here, an intercellular DNA‐reaction‐programmed ESPN ( e xpansion of s tem cells with p airing n iches) strategy is developed for 3D culture of mammary stem cells (MaSCs). Boolean logic operations are implemented to confer DNA‐programmed mechanical signaling and genetically engineered morphogen signaling by niche cells, resulting in sustained expansion of MaSCs in vitro. The creation of stem cell niches improves the proliferation of pluripotent cells by four times during one‐week culture. This method thus provides a novel approach for logical regulation of stemness and proliferation of stem cells for biomedicine.
    Type of Medium: Online Resource
    ISSN: 0935-9648 , 1521-4095
    URL: Issue
    URL: Article
    DOI: 10.1002/adma.v30.46
    DOI: 10.1002/adma.201804861
    RVK:
    UA 1538
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1474949-X
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  • 9
    Online Resource
    Online Resource
    FER promotes cell migration via regulating JNK activity
    Wiley ; 2019
    In:  Cell Proliferation Vol. 52, No. 5 ( 2019-09)
    Details
    In: Cell Proliferation, Wiley, Vol. 52, No. 5 ( 2019-09)
    Abstract: Cell migration has a key role in cancer metastasis, which contributes to drug resistance and tumour recurrence. Better understanding of the mechanisms involved in this process will potentially reveal new drug targets for cancer therapy. Fer is a non‐receptor protein tyrosine kinase aberrantly expressed in various human cancers, whereas its role in tumour progression remains elusive. Materials and Methods Transgenic flies and epigenetic analysis were employed to investigate the role of Drosophila Fer (FER) in cell migration and underlying mechanisms. Co‐immunoprecipitation assay was used to monitor the interaction between FER and Drosophila JNK (Bsk). The conservation of Fer in regulating JNK signalling was explored in mammalian cancer and non‐cancer cells. Results Overexpression of FER triggered cell migration and activated JNK signalling in the Drosophila wing disc. Upregulation and downregulation in the basal activity of Bsk exacerbated and eliminated FER‐mediated migration, respectively. In addition, loss of FER blocked signal transduction of the JNK pathway. Specifically, FER interacted with and promoted the activity of Bsk, which required both the kinase domain and the C‐terminal of Bsk. Lastly, Fer regulated JNK activities in mammalian cells. Conclusions Our study reveals FER as a positive regulator of JNK‐mediated cell migration and suggests its potential role as a therapeutic target for cancer metastasis.
    Type of Medium: Online Resource
    ISSN: 0960-7722 , 1365-2184
    URL: Issue
    URL: Article
    DOI: 10.1111/cpr.v52.5
    DOI: 10.1111/cpr.12656
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2019986-7
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    Live‐cell imaging of octaarginine‐modified polymer dots via single particle tracking
    Wiley ; 2019
    In:  Cell Proliferation Vol. 52, No. 2 ( 2019-03)
    Details
    In: Cell Proliferation, Wiley, Vol. 52, No. 2 ( 2019-03)
    Abstract: Nanocarriers can greatly enhance the cellular uptake of therapeutic agents to regulate cell proliferation and metabolism. Nevertheless, further application of nanocarriers is often limited by insufficient understanding of the mechanisms of their uptake and intracellular behaviour. Materials and methods Fluorescent polymer dots (Pdots) are coated with synthetic octaarginine peptides (R8) and are analysed for cellular uptake and intracellular transportation in HeLa cervical cancer cells via single particle tracking. Results Surface modification with the R8 peptide efficiently improves both cellular uptake and endosomal escape of Pdots. With single particle tracking, we capture the dynamic process of internalization and intracellular trafficking of R8‐Pdots, providing new insights into the mechanism of R8 in facilitating nanostructure‐based cellular delivery. Furthermore, our results reveal R8‐Pdots as a novel type of autophagy inducer. Conclusions This study provides new insights into R8‐mediated cellular uptake and endosomal escape of nanocarriers. It potentiates biological applications of Pdots in targeted cell imaging, drug delivery and gene regulation.
    Type of Medium: Online Resource
    ISSN: 0960-7722 , 1365-2184
    URL: Issue
    URL: Article
    DOI: 10.1111/cpr.2019.52.issue-2
    DOI: 10.1111/cpr.12556
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2019986-7
    SSG: 12
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