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  • 1
    Online Resource
    Online Resource
    Clinical characterization of NTCP deficiency in paediatric patients : A case‐control study based on SLC10A1 genotyping analysis
    Wiley ; 2021
    In:  Liver International Vol. 41, No. 11 ( 2021-11), p. 2720-2728
    Details
    In: Liver International, Wiley, Vol. 41, No. 11 ( 2021-11), p. 2720-2728
    Abstract: Na + ‐taurocholate cotransporting polypeptide deficiency (NTCPD) is a newly described disorder arising from biallelic mutations of the SLC10A1 gene. As a result of a lack of compelling evidence from case‐control studies, its genotypic and phenotypic features remain open for in‐depth investigation. This study aimed to explore the genotypic and clinical phenotypic characteristics of paediatric patients with NTCPD. The SLC10A1 genotypes of all NTCPD patients were confirmed by screening for the prevalent variant c.800C 〉 T and Sanger sequencing when necessary. The clinical presentations and laboratory changes were collected, reviewed and analysed, and then qualitatively and quantitatively compared with the relevant controls. A total of 113 paediatric NTCPD patients were diagnosed while c.374dupG and c.682_683delCT were detected as two novel pathogenic mutations. Hypercholanemia was observed in 99.12% of the patients. Indirect hyperbilirubinemia in affected neonates exhibited higher positive rates in comparison to controls. Moreover, transient cholestatic jaundice, elevated liver enzymes and 25‐hydroxyvitamin D (Vit D) deficiency during early infancy were more commonly observed in patients than in controls. All NTCPD patients exhibited favourable clinical outcomes as a result of symptomatic and supportive treatment. The findings enriched the SLC10A1 mutation spectrum and provided comprehensive insights into the phenotypic characteristics of NTCPD. NTCPD should be considered and SLC10A1 gene should be analysed in patients with above age‐dependent clinical features. Furthermore, over investigation and intervention should be avoided in the management of NTCPD patients.
    Type of Medium: Online Resource
    ISSN: 1478-3223 , 1478-3231
    URL: Issue
    URL: Article
    DOI: 10.1111/liv.v41.11
    DOI: 10.1111/liv.15031
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2124684-1
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  • 2
    Online Resource
    Online Resource
    Effects of leaf zeatin and zeatin riboside induced by different clipping heights on the regrowth capacity of ryegrass
    Wiley ; 2014
    In:  Ecological Research Vol. 29, No. 2 ( 2014-03), p. 167-180
    Details
    In: Ecological Research, Wiley, Vol. 29, No. 2 ( 2014-03), p. 167-180
    Abstract: The effect of clipping height on ryegrass regrowth was investigated by examining the roles of several plant hormones. Our study consisted of three treatment conditions: (1) darkness over whole plants, (2) darkness only over stubble leaf sheaths, and (3) light over whole plants. Results showed that under darkness over whole plant, low stubble height resulted in low leaf regrowth biomass. Similar leaf regrowth biomass was observed under conditions of darkness only over stubble leaf sheaths as well as light over whole plants. Each unit weight of stubble at different clipping heights has relatively similar potential of providing stored organic substance for leaf regrowth. Therefore, regrowth index, calculated as newly grown leaf biomass divided by unit stubble weight, was used to evaluate regrowth capacity at different clipping heights under minimal influence of organic substances stored in stubbles. Under light over whole plants and single clipping, low stubble height and high stubble height with root thinning resulted in low leaf biomass and high regrowth index. On the other hand, under light over whole plants and frequent clipping high leaf biomass and regrowth index were observed in high stubble height. In addition, we found that leaf zeatin and zeatin riboside (Z + ZR) affected ryegrass regrowth and that roots regulated leaf Z + ZR concentration. Thus, our results indicate that root‐derived cytokinin concentration in leaves influences ryegrass regrowth at different clipping heights.
    Type of Medium: Online Resource
    ISSN: 0912-3814 , 1440-1703
    URL: Article
    DOI: 10.1007/s11284-013-1107-0
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 2023900-2
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Activation of AMPK‐dependent autophagy in the nucleus accumbens opposes cocaine‐induced behaviors of mice
    Wiley ; 2020
    In:  Addiction Biology Vol. 25, No. 2 ( 2020-03)
    Details
    In: Addiction Biology, Wiley, Vol. 25, No. 2 ( 2020-03)
    Abstract: Cocaine is a strong central nervous system stimulant, which can induce drug addiction. Previous studies have reported that cocaine‐induced autophagy is involved in neuroinflammation and cell death. However, the role of autophagy in psychomotor sensitivity to cocaine has not been explored. Here, we reported that D 1 receptor ‐CaMKII‐AMPK‐FoxO3a signaling pathway was involved in acute cocaine application‐induced autophagy in the nucleus accumbens (NAc) both in vitro and in vivo . Furthermore, we found that knockdown of the ATG5 gene in the NAc augmented behavioral response to cocaine, and induction of autophagy in the NAc with rapamycin attenuated cocaine‐induced behavioral response, which was coincident with the alterations of dendritic spine density in neurons of NAc. These results suggest that cocaine exposure leads to the induction of autophagy, which is a protective mechanism against behavioral response to cocaine of male mice.
    Type of Medium: Online Resource
    ISSN: 1355-6215 , 1369-1600
    URL: Issue
    URL: Article
    DOI: 10.1111/adb.v25.2
    DOI: 10.1111/adb.12736
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 1495537-4
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  • 4
    Online Resource
    Online Resource
    Front Cover Image, Volume 116, Number 12, December 2019
    Wiley ; 2019
    In:  Biotechnology and Bioengineering Vol. 116, No. 12 ( 2019-12)
    Details
    In: Biotechnology and Bioengineering, Wiley, Vol. 116, No. 12 ( 2019-12)
    Type of Medium: Online Resource
    ISSN: 0006-3592 , 1097-0290
    URL: Issue
    URL: Article
    DOI: 10.1002/bit.v116.12
    DOI: 10.1002/bit.26770
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1480809-2
    detail.hit.zdb_id: 280318-5
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  • 5
    Online Resource
    Online Resource
    Metabolic engineering of a robust Escherichia coli strain with a dual protection system
    Wiley ; 2019
    In:  Biotechnology and Bioengineering Vol. 116, No. 12 ( 2019-12), p. 3333-3348
    Details
    In: Biotechnology and Bioengineering, Wiley, Vol. 116, No. 12 ( 2019-12), p. 3333-3348
    Abstract: Considerable attention has been given to the development of robust fermentation processes, but microbial contamination and phage infection remain deadly threats that need to be addressed. In this study, a robust Escherichia coli BL21(DE3) strain was successfully constructed by simultaneously introducing a nitrogen and phosphorus (N & P) system in combination with a CRISPR/Cas9 system. The N & P metabolic pathways were able to express formamidase and phosphite dehydrogenase in the host cell, thus enabled cell growth in auxotrophic 3‐( N ‐morpholino)propanesulfonic acid medium with formamide and phosphite as nitrogen and phosphorus sources, respectively. N & P metabolic pathways also allowed efficient expression of heterologous proteins, such as green fluorescent protein (GFP) and chitinase, while contaminating bacteria or yeast species could hardly survive in this medium. The host strain was further engineered by exploiting the CRISPR/Cas9 system to enhance the resistance against phage attack. The resultant strain was able to grow in the presence of T7 phage at a concentration of up to 2 × 10 7 plaque‐forming units/ml and produce GFP with a yield of up to 30 μg/10 9 colony‐forming units, exhibiting significant advantages over conventional engineered E. coli . This newly engineered, robust E. coli BL21(DE3) strain therefore shows great potential for future applications in industrial fermentation.
    Type of Medium: Online Resource
    ISSN: 0006-3592 , 1097-0290
    URL: Issue
    URL: Article
    DOI: 10.1002/bit.v116.12
    DOI: 10.1002/bit.27165
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1480809-2
    detail.hit.zdb_id: 280318-5
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Tumor Microenvironment‐Responsive Nanocarrier Based on VO x Nanozyme Amplify Oxidative Stress for Tumor Therapy
    Wiley ; 2023
    In:  Advanced Functional Materials Vol. 33, No. 30 ( 2023-07)
    Details
    In: Advanced Functional Materials, Wiley, Vol. 33, No. 30 ( 2023-07)
    Abstract: The construction of a novel nanocarrier that can break the redox balance in tumor cell is a promising anti‐tumor strategy. Herein, a tumor microenvironment (TME)‐responsive nanocarrier VC@Lipo is rationally designed by embedding ultrasmall VO x nanozyme and photosensitizer chlorin e6 (Ce6) into liposomes. The size of VC@Lipo nanocarrier is ≈35 nm and can be degraded in the weakly acidic environment of TME. The VO x nanozyme exhibits peroxidase‐like activity and generates highly toxic hydroxyl radical ∙OH through Fenton‐like reaction and 1 O 2 in the presence of H 2 O 2 independent of light, and more 1 O 2 can be generated by the photodynamic effect of Ce6. In addition, the VO x nanozyme can effectively deplete intracellular overexpressed glutathione (GSH) through redox reactions. In vivo experiments demonstrate that the nanocarrier shows excellent biocompatibility, presents the largest enrichment at the tumor site after 6 h of intravenous injection into mice with the highest tumor inhibition rate of 54.18% after laser irradiation. Compared with the single treatment mode, VC@Lipo shows the best synergistic effect of chemodynamic‐photodynamic therapy. This work provides a new paradigm for nanocatalytic therapy of cancer and is expected to provide new ideas for precision medicine in cancer.
    Type of Medium: Online Resource
    ISSN: 1616-301X , 1616-3028
    URL: Issue
    URL: Article
    DOI: 10.1002/adfm.v33.30
    DOI: 10.1002/adfm.202212740
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2029061-5
    detail.hit.zdb_id: 2039420-2
    SSG: 11
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  • 7
    Online Resource
    Online Resource
    Pulse oximetry could significantly enhance the early detection of critical congenital heart disease in neonatal intensive care units
    Wiley ; 2016
    In:  Acta Paediatrica Vol. 105, No. 11 ( 2016-11)
    Details
    In: Acta Paediatrica, Wiley, Vol. 105, No. 11 ( 2016-11)
    Abstract: Limited data have been available regarding critical congenital heart disease ( CHD ) screening in neonatal intensive care unit ( NICU s). This study evaluated the feasibility of screening for CHD by adding pulse oximetry ( POX ) to clinical evaluation in a NICU in Shanghai, China. Methods We screened 4128 eligible consecutive NICU admissions using POX plus clinical evaluation. Infants with positive screening results were then evaluated with echocardiography. Those with negative screening results were put under observation, and they also underwent echocardiography if their oxygen saturation fell below 95% on room air during hospitalisation. Results This enhanced procedure detected 19 critical CHD cases, and seven of these diagnoses would have been delayed if POX had not been incorporated into the screening strategy. This means that the addition of POX increased the detection rate of critical CHD from 63.2 to 100%. The false‐positive rate of critical CHD screening using POX plus clinical evaluation was higher in NICU patients with high morbidity rates. Conclusion When pulse oximetry screening was added to clinical evaluation, it increased the number of critical CHD cases that were detected in our NICU . This method could provide a useful screening protocol for critical CHD cases.
    Type of Medium: Online Resource
    ISSN: 0803-5253 , 1651-2227
    URL: Issue
    URL: Article
    DOI: 10.1111/apa.2016.105.issue-11
    DOI: 10.1111/apa.13553
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 1492629-5
    detail.hit.zdb_id: 1501466-6
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  • 8
    Online Resource
    Online Resource
    Matrine inhibits invasiveness and metastasis of human malignant melanoma cell line A375 in vitro
    Wiley ; 2008
    In:  International Journal of Dermatology Vol. 47, No. 5 ( 2008-05), p. 448-456
    Details
    In: International Journal of Dermatology, Wiley, Vol. 47, No. 5 ( 2008-05), p. 448-456
    Abstract: Background  Matrine is a traditional Chinese medicine with significant inhibitory activity against malignant tumors. Its effects on the invasiveness and metastasis of malignant tumors have rarely been reported. Aim  To investigate whether matrine can inhibit the metastasis‐related activities of the human malignant melanoma cell line A375 in vitro . Methods  3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) assay and Annexin‐V–fluorescein isothiocyanate/propidium iodide (Annexin‐V‐FITC/PI) affinity assay were used to examine the effects of matrine on the proliferation and apoptosis induction of A375 cells. The morphologic changes of A375 cells were observed by light and electron microscopy. Semiquantitative reverse transcription‐polymerase chain reaction (RT‐PCR) and Western blotting were performed to evaluate the expression of heparanase mRNA and protein. The effect of matrine on the adhesion ability and invasiveness of treated A375 cells was tested by cell–Matrigel adhesion assay and Matrigel invasion assay, respectively. Results  Matrine showed significant inhibition of the proliferation of A375 cells in a dose‐ and time‐dependent manner. It also induced apoptosis in a dose‐dependent manner. Compared with the control group, the levels of heparanase mRNA and protein expression of A375 cells treated with different concentrations of matrine were decreased significantly, as were their adhesion ability and invasiveness. Conclusions  These findings indicate that matrine inhibits the invasiveness and metastasis of A375 cells in vitro. The mechanisms may be linked to the inhibition of cellular proliferation, induction of apoptosis, and downregulation of heparanase mRNA and protein expression.
    Type of Medium: Online Resource
    ISSN: 0011-9059 , 1365-4632
    URL: Issue
    URL: Article
    DOI: 10.1111/ijd.2008.47.issue-5
    DOI: 10.1111/j.1365-4632.2008.03627.x
    Language: English
    Publisher: Wiley
    Publication Date: 2008
    detail.hit.zdb_id: 2020365-2
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  • 9
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    Online Resource
    Furanodienone inhibits cell proliferation and survival by suppressing ERα signaling in human breast cancer MCF‐7 cells
    Wiley ; 2011
    In:  Journal of Cellular Biochemistry Vol. 112, No. 1 ( 2011-01), p. 217-224
    Details
    In: Journal of Cellular Biochemistry, Wiley, Vol. 112, No. 1 ( 2011-01), p. 217-224
    Abstract: Estrogen receptor alpha (ERα) plays an important role in the development and progression of breast cancer and thus the attenuation of ERα activities is a promising treatment strategy. Furanodienone is one of the main bioactive chemical components of Rhizoma Curcumae which is commonly used in Chinese medicine for the treatment of cancer. In this study, we investigated the effects of furanodienone on human breast cancer MCF‐7, T47D, and MDA‐MB‐231 cells. Our results showed that furanodienone could inhibit MCF‐7, T47D, and MDA‐MB‐231 cells proliferation in a dose (10–160 µM) dependent manner. ERα‐negative MDA‐MB‐231 cells were less sensitive to furanodienone than ERα‐positive MCF‐7 and T47D cells. Furanodienone could effectively block 17β‐estradiol (E2)‐stimulated MCF‐7 cell proliferation and cell cycle progression and induce apoptosis evidenced by the flow cytometric detection of sub‐G1 DNA content and the appearance of apoptotic nuclei after DAPI staining. Furanodienone specifically down‐regulated ERα protein and mRNA expression levels without altering ERβ expression. Furanodienone treatment inhibited E2‐stimulation of estrogen response element (ERE)‐driven reporter plasmid activity and ablated E2‐targeted gene (e.g., c‐Myc, Bcl‐2, and cyclin D1) expression which resulted in the inhibition of cell cycle progression and cell proliferation, and in the induction of apoptosis. Knockdown of ERα in MCF‐7 cells by ERα‐specific siRNA decreased the cell growth inhibitory effect of furanodienone. These findings suggest that effects of furanodienone on MCF‐7 cells are mediated, at least in part, by inhibiting ERα signaling. J. Cell. Biochem. 112: 217–224, 2011. © 2010 Wiley‐Liss, Inc.
    Type of Medium: Online Resource
    ISSN: 0730-2312 , 1097-4644
    URL: Issue
    URL: Article
    DOI: 10.1002/jcb.v112.1
    DOI: 10.1002/jcb.22922
    Language: English
    Publisher: Wiley
    Publication Date: 2011
    detail.hit.zdb_id: 1479976-5
    SSG: 12
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  • 10
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    Online Resource
    Partially Reduced Graphene Oxide Membranes Crosslinked by an Anionic Porphyrin: Green Fabrication for High‐Performance Ion Sieving
    Wiley ; 2022
    In:  ChemistrySelect Vol. 7, No. 9 ( 2022-03-07)
    Details
    In: ChemistrySelect, Wiley, Vol. 7, No. 9 ( 2022-03-07)
    Abstract: GO membranes crosslinked by planar cation (e. g. TMPyP, a cationic porphyrin) meet the requirements of green fabrication, because TMPyP can pre‐assemble on GO sheets. However, their ion sieving performance are unsatisfactory. With shortened water channels, reduced graphene oxide (rGO) membranes owe high water flux, yet the retention of multivalent anions may goes down distinctly on account of Donnan effect decreasing obviously. Herein, we focus on the assembly between partially reduced graphene oxide ( p ‐rGO) and Anionic planar molecule (tetrasulfonic tetraphenyl porphyrin, TPPS), and then fabricate p ‐rGO/TPPS composite membranes via assembling p ‐rGO/TPPS sheets on base membrane. Anionic planar molecule is firstly used as crosslinking agent for graphene‐based membranes, which has the advantage of 100% atomic efficiency. The fabricated p ‐rGO/TPPS membranes show better sieving performance than GO/TMPyP membranes on both side of rejection of Na 2 SO 4 and water flux. Moreover, water flux go up to around another double though rejection decline slightly as the polyther sulfone (PES) base membrane with large surface pores is used.
    Type of Medium: Online Resource
    ISSN: 2365-6549 , 2365-6549
    URL: Issue
    URL: Article
    DOI: 10.1002/slct.v7.9
    DOI: 10.1002/slct.202103405
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2844262-3
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