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  • 1
    Online Resource
    Online Resource
    Innate immunity proteins and a new truncated form of SPLUNC1 in nasopharyngeal aspirates from infants with respiratory syncytial virus infection
    Wiley ; 2011
    In:  PROTEOMICS – Clinical Applications Vol. 5, No. 9-10 ( 2011-10), p. 513-522
    Details
    In: PROTEOMICS – Clinical Applications, Wiley, Vol. 5, No. 9-10 ( 2011-10), p. 513-522
    Abstract: Purpose : Respiratory syncytial virus (RSV) is the most common cause of severe respiratory tract infection in infants. The aim was to identify host defence components in nasopharyngeal aspirate (NPA) from infants with RSV infection and to study the expression of the novel 25 kDa innate immunity protein SPLUNC1. Experimental design : NPAs from infants were analyzed with 2‐DE and MS in a pilot study. The levels of SPLUNC1 were analyzed with immunoblotting in 47 NPAs, admitted for RSV diagnosis. Results : Totally, 35 proteins were identified in NPA, including several innate immunity proteins such as group X phospholipase A 2 , different S100 proteins and SPLUNC1. In addition, a new truncated 15 kDa form of SPLUNC1 was identified that was detected in about 50% of the aspirates admitted for RSV diagnosis. RSV‐positive boys had significantly less 25 kDa SPLUNC1 than RSV‐negative boys while there were no significant differences among girls. Conclusions and clinical relevance : Several important innate immunity proteins were identified in NPA. Notably, a new truncated form of the newly suggested anti‐bacterial protein SPLUNC1 was found. It is possible that a decrease in SPLUNC1 in the upper airways may increase the risk for severe pneumonia in boys.
    Type of Medium: Online Resource
    ISSN: 1862-8346 , 1862-8354
    URL: Issue
    URL: Article
    DOI: 10.1002/prca.v5.9/10
    DOI: 10.1002/prca.201100016
    Language: English
    Publisher: Wiley
    Publication Date: 2011
    detail.hit.zdb_id: 2317130-3
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  • 2
    Online Resource
    Online Resource
    Newly identified proteins in human nasal lavage fluid from non-smokers and smokers using two-dimensional gel electrophoresis and peptide mass fingerprinting
    Wiley ; 2002
    In:  PROTEOMICS Vol. 2, No. 1 ( 2002-01), p. 112-120
    Details
    In: PROTEOMICS, Wiley, Vol. 2, No. 1 ( 2002-01), p. 112-120
    Type of Medium: Online Resource
    ISSN: 1615-9853 , 1615-9861
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.1002/1615-9861(200201)2:1〈〉1.0.CO;2-Y
    DOI: 10.1002/(ISSN)1615-9861
    DOI: 10.1002/1615-9861(200201)2:1〈112::AID-PROT112〉3.0.CO;2-N
    Language: English
    Publisher: Wiley
    Publication Date: 2002
    detail.hit.zdb_id: 2037674-1
    SSG: 12
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  • 3
    Online Resource
    Online Resource
    Daytime changes of salivary biomarkers involved in pain
    Wiley ; 2020
    In:  Journal of Oral Rehabilitation Vol. 47, No. 7 ( 2020-07), p. 843-850
    Details
    In: Journal of Oral Rehabilitation, Wiley, Vol. 47, No. 7 ( 2020-07), p. 843-850
    Abstract: The study aimed to investigate salivary levels of nerve growth factor (NGF), brain‐derived neurotrophic factor (BDNF), substance P (SP) and glutamate at five time points from morning to afternoon in a well‐characterised healthy and pain‐free individuals. Ten young adults were included. Unstimulated and stimulated whole saliva were collected from each participant repeatedly across the day. Blood samples were drawn in connection with the first and last saliva sample as reference standard. Levels of NGF and BDNF were determined using gel‐free Western blot technology, glutamate levels were analysed using a colorimetric assay, and SP was determined using a commercially available ELISA. Salivary NGF and BDNF showed significant differences between the different collection times in both unstimulated (NGF; P  = .006; BDNF; P  = .026) and stimulated whole saliva (NGF; P  = .006; BDNF; P  = .019). The highest concentrations of the neuropeptides were expressed in the early morning, and they thereafter decreased across the day. In contrast, the expression of salivary glutamate and SP did not show any significant changes across the day. Plasma levels of NGF were higher in the evening sample ( P  = .028); otherwise, there were no significant differences for any of the other markers between morning and evening samples. NGF and BDNF in whole saliva showed a significant variation across the day. On the contrary, no variation in the levels of SP and glutamate was detected. These findings highlight the importance of consistency in the collection time and approach in biomarker studies using saliva.
    Type of Medium: Online Resource
    ISSN: 0305-182X , 1365-2842
    URL: Issue
    URL: Article
    DOI: 10.1111/joor.v47.7
    DOI: 10.1111/joor.12977
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2007587-X
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  • 4
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    Online Resource
    Mapping of proteins in human saliva using two‐dimensional gel electrophoresis and peptide mass fingerprinting
    Wiley ; 2003
    In:  PROTEOMICS Vol. 3, No. 6 ( 2003-06), p. 1003-1015
    Details
    In: PROTEOMICS, Wiley, Vol. 3, No. 6 ( 2003-06), p. 1003-1015
    Abstract: Human saliva contains a large number of proteins that can be used for diagnosis and are of great potential in clinical and epidemiological research. The aim of this work was to map the proteins in saliva by two‐dimensional gel electrophoresis (2‐DE), and to identify abundant proteins by peptide mass fingerprinting using trypsin cleavage and matrix‐assisted laser desorption/ionization‐time of flight‐mass spectrometry analysis. One hundred proteins were identified representing 20 different identities according to accession numbers. Abundant proteins expressed in different forms were: α‐amylase, immunoglobulin A, prolactin‐inducible protein, zinc‐α 2 ‐glycoprotein and cystatins (S, SA, D and SN). Other proteins found were interleukin‐1 receptor antagonist, von Ebner’s gland protein (lipocalin‐1) and calgranulin A and B (S100A8 and A9). Furthermore, apolipoprotein A‐I, β 2 ‐microglobulin, glutathione S‐transferase P and fatty acid‐binding protein were also identified. Our results show that human saliva contains a large number of proteins that are involved in inflammatory and immune responses. The 2‐DE protein map constructed opens the possibility to investigate protein changes associated with disease processes.
    Type of Medium: Online Resource
    ISSN: 1615-9853 , 1615-9861
    URL: Issue
    URL: Article
    DOI: 10.1002/pmic.v3:6
    DOI: 10.1002/pmic.200300426
    Language: English
    Publisher: Wiley
    Publication Date: 2003
    detail.hit.zdb_id: 2037674-1
    SSG: 12
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  • 5
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    Online Resource
    Myogenous temporomandibular disorders and salivary markers of oxidative stress—A cross‐sectional study
    Wiley ; 2021
    In:  Journal of Oral Rehabilitation Vol. 48, No. 1 ( 2021-01), p. 1-9
    Details
    In: Journal of Oral Rehabilitation, Wiley, Vol. 48, No. 1 ( 2021-01), p. 1-9
    Abstract: The clinical care of chronic pain requires personalised understanding of the mechanisms involved. Temporomandibular disorders (TMD) are the most common chronic orofacial pain conditions, and oxidative stress has been proposed to be implicated in their pathophysiology, especially in arthrogenous TMD. However, few studies have explored oxidative stress in myogenous TMD (TMDM). Objective The aims of this study were to compare the salivary oxidative stress profiles between individuals with TMDM and healthy controls, and to explore associations of these markers with clinical characteristics. Methodology Saliva samples were collected from 39 individuals with TMDM and 37 age and sex‐matched healthy volunteers. Psychological stress levels and clinical characteristics were assessed in all participants. The samples were analysed for total oxidant status (TOS), total antioxidative capacity (TAC) and superoxide dismutase activity (SODa). Comparisons between groups were performed using parametric and non‐parametric tests depending on data distribution. Results Psychological stress was higher in TMDM compared to controls ( P   〈  .001). TAC levels were significantly higher ( P   〈  .05) whereas TOS levels were significantly lower ( P   〈  .05) in TMDM compared to controls. There were no differences in SODa levels between groups and no correlations were found between clinical characteristics and oxidative stress markers. Conclusion Individuals with TMDM showed higher levels of antioxidative markers, but lower levels of oxidative markers. These results can be explained in part by chronicity and adaptation to the disease and other factors, such as psychological stress. Longitudinal studies must be conducted to clarify the role of oxidative stress in TMDM.
    Type of Medium: Online Resource
    ISSN: 0305-182X , 1365-2842
    URL: Issue
    URL: Article
    DOI: 10.1111/joor.v48.1
    DOI: 10.1111/joor.13100
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2007587-X
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