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  • 1
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    Online Resource
    Neuroglobin expression and function in the temporal cortex of bilirubin encephalopathy rats
    Wiley ; 2022
    In:  The Anatomical Record Vol. 305, No. 2 ( 2022-02), p. 254-264
    Details
    In: The Anatomical Record, Wiley, Vol. 305, No. 2 ( 2022-02), p. 254-264
    Abstract: Bilirubin encephalopathy (BE) is a neurological syndrome in newborns, mainly caused by neuronal injury due to excessive oxidative stress produced by unconjugated bilirubin (UCB). Neuroglobin (NGB) can protect the brain by removing oxidative stress species, but its expression and significance in BE are not clear. To address this question, the neonatal BE model was established by injecting UCB into the cerebellomedullary cistern of 7‐day‐old SD rats. Rats were divided into a sham and BE 6 hr group, BE 12 hr group, BE 24 hr group, and BE 7 d group according to UCB action times. Hematoxylin/eosin and Nissl staining, and electron microscopy were employed to observe the pathological and ultrastructural changes of nerve cells in each group. Immunofluorescence staining was used to detect NGB expression sites and cell types. Western blotting and quantitative PCR served to detect NGB expression and test the mitochondrial apoptosis signal pathway. The results confirm that UCB can lead to pathological damage and ultrastructural changes in rats' temporal cortex, increasing the expression of apoptosis‐related proteins Bax, Bcl‐2, Cyt c, Caspase‐3, and neuronal NGB. UCB promotes NGB expression with an increase in action time and reach a peak at 12 hr. In summary, brain damage induced by UCB will cause an increase in NGB expression, the increasing NGB can inhibit neuron apoptosis in early BE phases. Therefore, promoting the expression of endogenous NGB, to act as a neuroprotective agent may be a potential treatment strategy for BE.
    Type of Medium: Online Resource
    ISSN: 1932-8486 , 1932-8494
    URL: Issue
    URL: Article
    DOI: 10.1002/ar.v305.2
    DOI: 10.1002/ar.24734
    Language: English
    Publisher: Wiley
    Publication Date: 2022
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    detail.hit.zdb_id: 2109216-3
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  • 2
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    Curcumin Ameliorates Memory Deficits by Enhancing Lactate Content and MCT2 Expression in APP/PS1 Transgenic Mouse Model of Alzheimer's Disease
    Wiley ; 2019
    In:  The Anatomical Record Vol. 302, No. 2 ( 2019-02), p. 332-338
    Details
    In: The Anatomical Record, Wiley, Vol. 302, No. 2 ( 2019-02), p. 332-338
    Abstract: Curcumin is a natural product with several anti‐Alzheimer's disease (AD) neuroprotective properties. This study aimed to investigate the effects of curcumin on memory deficits, lactate content, and monocarboxylate transporter 2 (MCT2) in APP/PS1 mouse model of AD. APP/PS1 transgenic mice and wild‐type (WT) C57BL/6J mice were used in the present study. Spatial learning and memory of the mice was detected using Morris water‐maze test. Cerebral cortex and hippocampus lactate contents were detected using lactate assay. MCT2 expression in the cerebral cortex and hippocampus was examined by immunohistochemistry and Western blotting. Results showed that spatial learning and memory deficits were improved in curcumin‐treated APP/PS1 mouse group compared with those in APP/PS1 mice group. Brain lactate content and MCT2 protein level were increased in curcumin‐treated APP/PS1 mice than in APP/PS1 mice. In summary, our findings indicate that curcumin could ameliorate memory impairments in APP/PS1 mouse model of AD. This phenomenon may be at least partially due to its improving effect on the lactate content and MCT2 protein expression in the brain. Anat Rec, 302:332–338, 2019. © 2018 Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 1932-8486 , 1932-8494
    URL: Issue
    URL: Article
    DOI: 10.1002/ar.v302.2
    DOI: 10.1002/ar.23969
    Language: English
    Publisher: Wiley
    Publication Date: 2019
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  • 3
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    Internalization of Aquaporin‐4 After Collagenase‐Induced Intracerebral Hemorrhage
    Wiley ; 2015
    In:  The Anatomical Record Vol. 298, No. 3 ( 2015-03), p. 554-561
    Details
    In: The Anatomical Record, Wiley, Vol. 298, No. 3 ( 2015-03), p. 554-561
    Abstract: Brain edema formation following intracerebral hemorrhage (ICH) appears to be related with aquaporin‐4 (AQP4), which is critically involved in brain volume homeostasis and water balance. Despite its importance, the regulation of AQP4 expression involved in transmembrane water movements still remains rudimentary. Many studies suggest that the internalization of several membrane‐bound proteins, including AQP4, may occur with or without lysosomal degradation. Previously, we investigated the internalization of AQP4 in retinal ischemic‐reperfusion model. Here, we test the hypothesis that AQP4 is internalized post‐ICH and then degraded in the lysosome. The results demonstrated that both AQP4 and the mannose‐6‐phosphate receptor (MPR) co‐localized in perihematomal region at 6 hr post‐ICH. In addition, AQP4 and lysosomal‐associated membrane protein 1 (LAMP1) also co‐localized in perihematomal region, with co‐expression increasing followed by a gradual decrease at different time windows post‐ICH (6, 12, 24, 48, and 72 hr). After ICH, the Evans blue leakage happened very early at 1 hr and the brain swelling occurred at 3 hr. Moreover, we also found the AQP4 mRNA and AQP4 protein were increased post‐ICH. These results suggest that AQP4 is internalized and the lysosome is involved in degrading the internalized AQP4 post‐ICH. Both the AQP4 internalization and lysosomal degradation may provide biophysical insights regarding the potential of new treatments for brain edema. Anat Rec, 298:554–561, 2015. © 2014 Wiley Periodicals, Inc.
    Type of Medium: Online Resource
    ISSN: 1932-8486 , 1932-8494
    URL: Issue
    URL: Article
    DOI: 10.1002/ar.v298.3
    DOI: 10.1002/ar.23055
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2273240-8
    detail.hit.zdb_id: 2109216-3
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  • 4
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    Steroids Enable Mesenchymal Stromal Cells to Promote CD8 + T Cell Proliferation Via VEGF‐C
    Wiley ; 2021
    In:  Advanced Science Vol. 8, No. 12 ( 2021-06)
    Details
    In: Advanced Science, Wiley, Vol. 8, No. 12 ( 2021-06)
    Abstract: Mesenchymal stromal cells (MSCs) function as a formidable regulator of inflammation and tissue homeostasis and expanded MSCs are shown to be effective in treating various inflammatory diseases. Their therapeutic effects require the existence of certain inflammatory cytokines. However, in the absence of sufficient proinflammatory stimuli or in the presence of anti‐inflammatory medications, MSCs are animated to promote immune responses and unable to alleviate inflammatory disorders. In this study, it is demonstrated that steroid co‐administration interferes the efficacy of MSCs in treating acute graft‐versus‐host disease (aGvHD). Molecular analysis reveals that vascular endothelial growth factor C (VEGF‐C) is highly induced in MSCs by steroids and TNF α and VEGF‐C in turn promotes CD8 + T cell response. This immune promoting effect is abolished by blockade or specific genetic ablation of VEGFR3 in CD8 + T cells. Additionally, administration of VEGF‐C alone exacerbates aGvHD progression through eliciting more vigorous CD8 + T cell activation and proliferation. Further studies demonstrate that VEGF‐C augments the PI3K/AKT signaling process and the expression of downstream genes, such as Cyclin D1. Thus, the data demonstrate that steroids can reverse the immunosuppressive effect of MSCs via promoting VEGF‐C‐augmented CD8 + T cell response and provide novel information for designing efficacious MSC‐based therapies.
    Type of Medium: Online Resource
    ISSN: 2198-3844 , 2198-3844
    URL: Issue
    URL: Article
    DOI: 10.1002/advs.v8.12
    DOI: 10.1002/advs.202003712
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2808093-2
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  • 5
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    Online Resource
    BCL 2 A 1 is a Potential Biomarker for Postoperative Seizure Control in Patients with Low‐grade Gliomas
    Wiley ; 2013
    In:  CNS Neuroscience & Therapeutics Vol. 19, No. 11 ( 2013-11), p. 882-888
    Details
    In: CNS Neuroscience & Therapeutics, Wiley, Vol. 19, No. 11 ( 2013-11), p. 882-888
    Abstract: To identify molecular genetic factors that influence preoperative seizure occurrence and postoperative seizure control in patients with low‐grade gliomas ( LGG s). Methods Fifty‐four WHO grade II astrocytomas were used for microarray analysis under strict inclusion criteria. The primary endpoint was seizure control at 12 months after surgery. Biological processes were investigated by gene ontology ( GO ) analysis. Quantitative RT ‐ PCR and immunohistochemistry were used to validate key genes. Results Differentially expressed genes correlated with seizure occurrence failed to significantly distinguish patients with and without a history of seizures. With respect to postoperative seizure control, a transcript profile of 92 genes was identified, which successfully separated patients with good and poor seizure prognosis. GO analysis revealed that the most striking overrepresentation of genes was found in a category of anti‐apoptotic genes and their regulation. Increased expression was also observed for genes involved in immune and inflammatory responses. BCL 2A1 was proven to be a novel marker associated with seizure prognosis. Conclusion Increased anti‐apoptotic activity of tumor cells appears to contribute to seizure recurrence after surgery in patients with LGG s. These findings provide insights that may lead to the development of effective treatment strategies for prolonging the survival of patients with LGG in the future.
    Type of Medium: Online Resource
    ISSN: 1755-5930 , 1755-5949
    URL: Issue
    URL: Article
    DOI: 10.1111/cns.2013.19.issue-11
    DOI: 10.1111/cns.12148
    Language: English
    Publisher: Wiley
    Publication Date: 2013
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  • 6
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    Coseismic Slip Distribution of the 2001 Kunlun Mountain Pass West Earthquake Constrained by GPS and Insar Data
    Wiley ; 2008
    In:  Chinese Journal of Geophysics Vol. 51, No. 4 ( 2008-07), p. 753-764
    Details
    In: Chinese Journal of Geophysics, Wiley, Vol. 51, No. 4 ( 2008-07), p. 753-764
    Type of Medium: Online Resource
    ISSN: 0898-9591
    URL: Issue
    URL: Article
    DOI: 10.1002/cjg2.v51.4
    DOI: 10.1002/cjg2.1268
    Language: English
    Publisher: Wiley
    Publication Date: 2008
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  • 7
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    Online Resource
    First‐Principles Study of Structural, Electronic, and Elastic Properties of Sb 2 S 3 under Pressure
    Wiley ; 2022
    In:  physica status solidi (b) Vol. 259, No. 3 ( 2022-03)
    Details
    In: physica status solidi (b), Wiley, Vol. 259, No. 3 ( 2022-03)
    Abstract: The effects of pressure on the lattice parameters, electronic properties and elastic properties of Sb 2 S 3 under 0–30 GPa are studied by first‐principles calculation. The calculated results of structural parameters of generalized gradient approximation (GGA)‐Perdew–Burke–Ernzerhof (PBE) are less than 3% different from the previous experimental values. With the increase of pressure, the bandgap of Sb 2 S 3 becomes smaller and smaller. At a pressure of 35 GPa, the conduction band coincides with the valence band. Metallization will occur. The Poisson's ratio results show that the Poisson's ratio of Sb 2 S 3 crystal is 0.2892 at 10 GPa. When the pressure increases to 30 GPa, the mechanical stability criterion is no longer satisfied, and the mechanical properties will be unstable. At atmospheric pressure, there is no virtual screen in the phonon dispersion spectrum of Sb 2 S 3 . At 30 GPa, the phonon appears virtual screen and the structure is unstable. This is consistent with the results of the previous mechanical stability calculation. Finally, the wave velocity and anisotropy of Sb 2 S 3 are calculated by its elastic coefficients. All the conclusions, herein, can provide a theoretical basis for the future study of Sb 2 S 3 under high pressure. So, it is necessary to study the skills of Sb 2 S 3 under high pressure.
    Type of Medium: Online Resource
    ISSN: 0370-1972 , 1521-3951
    URL: Issue
    URL: Article
    DOI: 10.1002/pssb.v259.3
    DOI: 10.1002/pssb.202100234
    Language: English
    Publisher: Wiley
    Publication Date: 2022
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    detail.hit.zdb_id: 1481096-7
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  • 8
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    ZPAC is required for normal spermatogenesis in mice
    Wiley ; 2015
    In:  Molecular Reproduction and Development Vol. 82, No. 10 ( 2015-10), p. 747-755
    Details
    In: Molecular Reproduction and Development, Wiley, Vol. 82, No. 10 ( 2015-10), p. 747-755
    Abstract: The ubiquitin‐proteasome pathway, involved in genetic recombination and sex‐chromosome silencing during meiosis, plays critical roles in the specification of germ‐line stem cells and the differentiation of gametes from gonocytes. Zygote‐specific proteasome assembly chaperone (ZPAC) is expressed in the early mouse embryo, where it is important for progression of the mouse maternal‐to‐zygotic transition. The role of ZPAC during spermatogenesis in the adult gonads, however, remains unknown. In this study, rapid amplification of cDNA ends was used to determine the Zpac cDNA sequence, a 1584‐bp transcript that includes a putative 1122‐bp open reading frame coding for a 373 amino acid protein. Western blot and immunohistochemistry revealed that ZPAC was specifically expressed in gonads. To further dissect the function of ZPAC during spermatogenesis, we employed PiggyBac‐based RNA interference vectors for transgenesis combined with cell transplantation to deplete Zpac during spermatogenesis. This RNAi‐mediate depletion in Zpac expression disrupted normal spermatogenesis from spermatogonial stem cells. Two independent yeast two‐hybrid screens further revealed an interaction between ZPAC and SYCE1. Together, these data suggest that ZPAC is required for normal spermatogenesis in mice. Mol. Reprod. Dev. 82: 747–755, 2015. © 2015 Wiley Periodicals, Inc .
    Type of Medium: Online Resource
    ISSN: 1040-452X , 1098-2795
    URL: Issue
    URL: Article
    DOI: 10.1002/mrd.v82.10
    DOI: 10.1002/mrd.22507
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 1493888-1
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  • 9
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    Dynamics of peripheral immune cells and their HLA‐G and receptor expressions in a patient suffering from critical COVID‐19 pneumonia to convalescence
    Wiley ; 2020
    In:  Clinical & Translational Immunology Vol. 9, No. 5 ( 2020-01)
    Details
    In: Clinical & Translational Immunology, Wiley, Vol. 9, No. 5 ( 2020-01)
    Abstract: Host immune responses are indispensable to combat the disease. We report the dynamics of peripheral immune cells, cytokines, and human leucocyte antigen‐G (HLA‐G) and its receptor expressions in a patient suffering from critical COVID‐19 pneumonia to convalescence. Methods Clinical data of the patient were collected from medical records. The expressions of HLA‐G and receptors ILT2, ILT4 and KIR2DL4 in peripheral immune cells were measured with flow cytometry. Results From critical COVID‐19 to the convalescent stage, early lymphopenia was improved (median: 0.6 × 10 9  L −1 vs. 0.9 × 10 9  L −1 , P  = 0.009), and an obvious fluctuation in WBC and neutrophil counts was observed. Initially, low levels of CD4 + T cells (from 120 to 528 μL −1 ) and CD8 + T cells (from 68 to 362 μL −1 ) gradually increased to normal levels. Meanwhile, high IL‐6 (from 251.8 to 6.32 pg mL −1 ), IL‐10 (from 39.53 to 5.21 pg mL −1 ) and IFN‐γ (from 13.55 to 3.16 pg mL −1 ) levels decreased, and IL‐4 (from 2.36 to 3.19 pg mL −1 ) and TNF‐α (from 2.27 to 20.2 pg mL −1 ) levels increased quickly when the viral RNA returned negative. Moreover, the percentage of HLA‐G + T cells, B cells and monocytes follows high–low–high pattern, while the percentage of receptors ILT2‐, ILT4‐ and KIR2DL4‐expressing cells remained relatively stable. Conclusion Our findings provide valuable information on the dynamics of early peripheral immunological responses in SARS‐CoV‐2 infection. CD4 + and CD8 + T cells, cytokines and HLA‐G + immune cells are associated with the natural history of the critical COVID‐19 patient; however, future studies are necessary.
    Type of Medium: Online Resource
    ISSN: 2050-0068 , 2050-0068
    URL: Issue
    URL: Article
    DOI: 10.1002/cti2.v9.5
    DOI: 10.1002/cti2.1128
    Language: English
    Publisher: Wiley
    Publication Date: 2020
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  • 10
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    Relocation of Small EarthquakesiIn Western Sichuan, China and Its Implications for Active Tectonics
    Wiley ; 2005
    In:  Chinese Journal of Geophysics Vol. 48, No. 3 ( 2005-05), p. 692-700
    Details
    In: Chinese Journal of Geophysics, Wiley, Vol. 48, No. 3 ( 2005-05), p. 692-700
    Type of Medium: Online Resource
    ISSN: 0898-9591
    URL: Article
    DOI: 10.1002/cjg2.702
    Language: English
    Publisher: Wiley
    Publication Date: 2005
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