In:
The Journal of Gene Medicine, Wiley, Vol. 6, No. 10 ( 2004-10), p. 1159-1169
Abstract:
Advanced gene therapy, tissue engineering and biopharmaceutical manufacturing require sophisticated and well‐balanced multiregulated multigene interventions to reprogram desired mammalian cell phenotypes. Methods We have combined the streptogramin (PIP)‐ and tetracycline (TET)‐responsive gene regulation systems for independent expression control of the differentiation determinants myoD and msx1 in C2C12‐derived cells. Results Different dual‐regulated expression scenarios which induce either both, only one or none of the lineage control genes triggered differential differentiation and precise control of myogenic, osteogenic or adipogenic cell phenotypes. Conclusions Our findings substantiate the use of multiregulated multigene interventions in reprogramming cellular differentiation pathways in a desired manner. Copyright © 2004 John Wiley & Sons, Ltd.
Type of Medium:
Online Resource
ISSN:
1099-498X
,
1521-2254
Language:
English
Publisher:
Wiley
Publication Date:
2004
detail.hit.zdb_id:
2002203-7
SSG:
12
Permalink