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  • 1
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    Online Resource
    MNS1 promotes hepatocarcinogenesis and metastasis via activating PI3K/AKT by translocating β‐catenin and predicts poor prognosis
    Wiley ; 2021
    In:  Liver International Vol. 41, No. 6 ( 2021-06), p. 1409-1420
    Details
    In: Liver International, Wiley, Vol. 41, No. 6 ( 2021-06), p. 1409-1420
    Abstract: Hepatocellular carcinoma (HCC) is a fatal disease characterized by vast molecular heterogeneity. Although major advances in tumour genetics has led to the identification of new biomarkers, the prognosis of patients with HCC remains dismal. Methods Quantitative reverse‐transcription polymerase chain reaction (qRT‐PCR) and western blot (WB) were used to evaluate meiosis‐specific nuclear structural 1 (MNS1) expression in HCC cells. Immunohistochemistry staining was used to evaluate MNS1 expression in HCC tissues. Clinical significance of MNS1 was evaluated by Cox regression analysis. Transwell assays were conducted to assess cells migration ability. Cell counting kit‐8 and colony formation assays were performed to detect cells proliferation ability. NOD/SCID/γc(null) (NOG) mice model was adopted to investigate functions of MNS1 in vivo. Results The expression of MNS1, which is elevated in most HCC tissues, correlated with poor survival in HCC patients. Functional experiments revealed the oncogenic role of MNS1, which promotes HCC growth and metastasis through AKT‐dependent modulation of β‐catenin. β‐Catenin expression was crucial for MNS1's oncogenic effects. MNS1 indirectly translocated β‐catenin from the cytoplasm to the nucleus via the MNS1‐GSK3β axis. Conclusions MNS1 promotes HCC growth and metastasis via activating PI3K/AKT signalling and may serve as an important prognostic biomarker as well as potential novel therapeutic target for HCC.
    Type of Medium: Online Resource
    ISSN: 1478-3223 , 1478-3231
    URL: Issue
    URL: Article
    DOI: 10.1111/liv.v41.6
    DOI: 10.1111/liv.14803
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2124684-1
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  • 2
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    New insight into BIRC3: A novel prognostic indicator and a potential therapeutic target for liver cancer
    Wiley ; 2019
    In:  Journal of Cellular Biochemistry Vol. 120, No. 4 ( 2019-04), p. 6035-6045
    Details
    In: Journal of Cellular Biochemistry, Wiley, Vol. 120, No. 4 ( 2019-04), p. 6035-6045
    Abstract: Prognosis of hepatocellular carcinoma (HCC) remains poor due to high recurrence rate and ineffective treatment options, highlighting the need to better understand the mechanism of recurrence and metastasis in HCC. Methods We first collected messenger RNA (mRNA) expression data from 442 cases of HCC patients from The Cancer Genome Atlas (TCGA) database as well as 251 HCC patients from Zhongshan Hospital during 2009 and 2010 to analyze the expression pattern from tissue microarray (TMA) of baculoviral IAP repeat containing 3 (BIRC3). Then, we used BIRC3 gain‐of‐function (overexpression) and loss‐of‐function (knockdown) studies to examine the effect of BIRC3 on HCC cell proliferation and invasion. In addition, we also investigated the undying mechanism by which BIRC3 contributes to HCC tumor progression. Functionally, we also used a BIRC3‐specific inhibitor AT‐406 in HCC xenograft model to explore the potential therapeutic benefit of targeting BIRC3 in liver cancer. Results BIRC3 serves as a novel prognostic indicator for HCC patients undergoing curative resection. BIRC3 promotes HCC epithelial‐mesenchymal transition (EMT), cell migration, and metastasis via upregulating MAP3K7, therefore, inducing ERK1/2 phosphorylation. The specific BIRC3 inhibitor AT‐406 can inhibit HCC cell proliferation and reduce pulmonary metastases. Conclusion BIRC3 induces tumor proliferation and metastasis in vitro and in vivo. BIRC3 may serve as a novel therapeutic target for liver cancer.
    Type of Medium: Online Resource
    ISSN: 0730-2312 , 1097-4644
    URL: Issue
    URL: Article
    DOI: 10.1002/jcb.v120.4
    DOI: 10.1002/jcb.27890
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1479976-5
    SSG: 12
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  • 3
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    Recent Advances on Heterojunction‐Type Anode Materials for Lithium‐/Sodium‐Ion Batteries
    Wiley ; 2022
    In:  Small Methods Vol. 6, No. 12 ( 2022-12)
    Details
    In: Small Methods, Wiley, Vol. 6, No. 12 ( 2022-12)
    Abstract: Rechargeable batteries are key in the field of electrochemical energy storage, and the development of advanced electrode materials is essential to meet the increasing demand of electrochemical energy storage devices with higher density of energy and power. Anode materials are the key components of batteries. However, the anode materials still suffer from several challenges such as low rate capability and poor cycling stability, limiting the development of high‐energy and high‐power batteries. In recent years, heterojunctions have received increasing attention from researchers as an emerging material, because the constructed heterostructures can significantly improve the rate capability and cycling stability of the materials. Although many research progress has been made in this field, it still lacks review articles that summarize this field in detail. Herein, this review presents the recent research progress of heterojunction‐type anode materials, focusing on the application of various types of heterojunctions in lithium/sodium‐ion batteries. Finally, the heterojunctions introduced in this review are summarized, and their future development is anticipated.
    Type of Medium: Online Resource
    ISSN: 2366-9608 , 2366-9608
    URL: Issue
    URL: Article
    DOI: 10.1002/smtd.v6.12
    DOI: 10.1002/smtd.202201025
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2884448-8
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  • 4
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    Development and characterization of novel EST‐SSR markers for Speranskia tuberculata (Euphorbiaceae)
    Wiley ; 2016
    In:  Applications in Plant Sciences Vol. 4, No. 10 ( 2016-10)
    Details
    In: Applications in Plant Sciences, Wiley, Vol. 4, No. 10 ( 2016-10)
    Abstract: The first set of expressed sequence tag–simple sequence repeat (EST‐SSR) markers were developed and characterized for Speranskia tuberculata (Euphorbiaceae), a traditional medicinal plant endemic to northern China, to explore the effects of recent habitat fragmentation on the genetic diversity and structure of this species. Methods and Results: In this study, a total of 18 novel polymorphic microsatellite (EST‐SSR) markers were developed for S. tuberculata using high‐throughput transcriptome sequencing. Analysis of 24 individuals of S. tuberculata from four natural populations revealed their robust polymorphic reliability. The number of alleles per locus ranged from two to 11, while the expected and observed heterozygosity per marker varied from 0.187 to 0.827 and 0.042 to 0.917, respectively. Of these markers, 13 showed good amplification results in the closely related species S. cantonensis . Conclusions: These newly generated SSR markers are expected to provide novel tools for genetic studies of S. tuberculata , which will contribute to the conservation and sustainable use of the species’ wild genetic resources.
    Type of Medium: Online Resource
    ISSN: 2168-0450 , 2168-0450
    URL: Article
    DOI: 10.3732/apps.1600067
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2699923-7
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  • 5
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    KDM5A silencing transcriptionally suppresses the FXYD3‐PI3K/AKT axis to inhibit angiogenesis in hepatocellular cancer via miR‐433 up‐regulation
    Wiley ; 2021
    In:  Journal of Cellular and Molecular Medicine Vol. 25, No. 8 ( 2021-04), p. 4040-4052
    Details
    In: Journal of Cellular and Molecular Medicine, Wiley, Vol. 25, No. 8 ( 2021-04), p. 4040-4052
    Abstract: Hepatocellular cancer (HCC) has been reported to belong to one of the highly vascularized solid tumours accompanied with angiogenesis of human umbilical vein endothelial cells (HUVECs). KDM5A, an attractive drug target, plays a critical role in diverse physiological processes. Thus, this study aims to investigate its role in angiogenesis and underlying mechanisms in HCC. ChIP‐qPCR was utilized to validate enrichment of H3K4me3 and KDM5A on the promotor region of miR‐433, while dual luciferase assay was carried out to confirm the targeting relationship between miR‐433 and FXYD3. Scratch assay, transwell assay, Edu assay, pseudo‐tube formation assay and mice with xenografted tumours were conducted to investigate the physiological function of KDM5A‐miR‐433‐FXYD3‐PI3K‐AKT axis in the progression of HCC after loss‐ and gain‐function assays. KDM5A p‐p85 and p‐AKT were highly expressed but miR‐433 was down‐regulated in HCC tissues and cell lines. Depletion of KDM5A led to reduced migrative, invasive and proliferative capacities in HCC cells, including growth and a lowered HUVEC angiogenic capacity in vitro. Furthermore, KDM5A suppressed the expression of miR‐433 by demethylating H3K4me3 on its promoterregion. miR‐433 negatively targeted FXYD3. Depleting miR‐433 or re‐expressing FXYD3 restores the reduced migrative, invasive and proliferative capacities, and lowers the HUVEC angiogenic capacity caused by silencing KDM5A. Therefore, KDM5A silencing significantly suppresses HCC tumorigenesis in vivo, accompanied with down‐regulated miR‐433 and up‐regulated FXYD3‐PI3K‐AKT axis in tumour tissues. Lastly, KDM5A activates the FXYD3‐PI3K‐AKT axis to enhance angiogenesis in HCC by suppressing miR‐433.
    Type of Medium: Online Resource
    ISSN: 1582-1838 , 1582-4934
    URL: Issue
    URL: Article
    DOI: 10.1111/jcmm.v25.8
    DOI: 10.1111/jcmm.16371
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2076114-4
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  • 6
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    Cancer‐associated fibroblast‐derived exosomal microRNA‐24‐3p enhances colon cancer cell resistance to MTX by down‐regulating CDX2/HEPH axis
    Wiley ; 2021
    In:  Journal of Cellular and Molecular Medicine Vol. 25, No. 8 ( 2021-04), p. 3699-3713
    Details
    In: Journal of Cellular and Molecular Medicine, Wiley, Vol. 25, No. 8 ( 2021-04), p. 3699-3713
    Abstract: MicroRNA‐24‐3p (miR‐24‐3p) has been implicated as a key promoter of chemotherapy resistance in numerous cancers. Meanwhile, cancer‐associated fibroblasts (CAFs) can secret exosomes to transfer miRNAs, which mediate tumour development. However, little is known regarding the molecular mechanism of CAF‐derived exosomal miR‐24‐3p in colon cancer (CC). Hence, this study intended to characterize the functional relevance of CAF‐derived exosomal miR‐24‐3p in CC cell resistance to methotrexate (MTX). We identified differentially expressed HEPH, CDX2 and miR‐24‐3p in CC through bioinformatics analyses, and validated their expression in CC tissues and cells. The relationship among HEPH, CDX2 and miR‐24‐3p was verified using ChIP and dual‐luciferase reporter gene assays. Exosomes were isolated from miR‐24‐3p inhibitor–treated CAFs (CAFs‐exo/miR‐24‐3p inhibitor), which were used in combination with gain‐of‐function and loss‐of‐function experiments and MTX treatment. CCK‐8, flow cytometry and colony formation assays were conducted to determine cell viability, apoptosis and colony formation, respectively. Based on the findings, CC tissues and cells presented with high expression of miR‐24‐3p and low expression of HEPH and CDX2. CDX2 was a target gene of miR‐24‐3p and could up‐regulate HEPH. Under MTX treatment, overexpressed CDX2 or HEPH and down‐regulated miR‐24‐3p reduced cell viability and colony formation and elevated cell apoptosis. Furthermore, miR‐24‐3p was transferred into CC cells via CAF‐derived exosomes. CAF‐derived exosomal miR‐24‐3p inhibitor diminished cell viability and colony formation and increased cell apoptosis in vitro and inhibited tumour growth in vivo under MTX treatment. Altogether, CAF‐derived exosomal miR‐24‐3p accelerated resistance of CC cells to MTX by down‐regulating CDX2/HEPH axis.
    Type of Medium: Online Resource
    ISSN: 1582-1838 , 1582-4934
    URL: Issue
    URL: Article
    DOI: 10.1111/jcmm.v25.8
    DOI: 10.1111/jcmm.15765
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2076114-4
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  • 7
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    Association of regular aerobic exercises and neuromuscular junction variants with incidence of frailty: an analysis of the Chinese Longitudinal Health and Longevity Survey
    Wiley ; 2021
    In:  Journal of Cachexia, Sarcopenia and Muscle Vol. 12, No. 2 ( 2021-04), p. 350-357
    Details
    In: Journal of Cachexia, Sarcopenia and Muscle, Wiley, Vol. 12, No. 2 ( 2021-04), p. 350-357
    Abstract: Candidate genes of neuromuscular junction (NMJ) pathway increased risk of frailty, but the extent and whether can be offset by exercises was unclear. The aim of this study was to investigate the association between aerobic exercises and incident frailty regardless of NMJ pathway‐related genetic risk. Methods A cohort study on participants from Chinese Longitudinal Healthy Longevity Survey was conducted from 2008 to 2011. A total of 7006 participants (mean age of 80.6 ± 10.3 years) without frailty at baseline were interviewed to record aerobic exercise status, and 4053 individuals among them submitted saliva samples. NMJ pathway‐related genes were genotyped and weighted genetic risk scores were constructed. Results During a median follow‐up of 3.1 years (19 634 person‐years), there were 1345 cases (19.2%) of incident frailty. Persistent aerobic exercises were associated with a 26% lesser frailty risk [adjusted hazard ratio ( HR ) = 0.74, 95% confidence interval ( CI ) = 0.64–0.85]. This association was stronger in a subgroup of 1552 longevous participants (age between 90 and 111 years, adjusted HR  = 0.72, 95% CI  = 0.60–0.87). High genetic risk was associated with a 35% increased risk of frailty (adjusted HR  = 1.35, 95% CI  = 1.16–1.58). Of the participants with high genetic risk and no persistent aerobic exercises, there was a 59% increased risk of frailty (adjusted HR  = 1.59, 95% CI  = 1.20–2.09). HRs for the risk of frailty increased from the low genetic risk with persistent aerobic exercise to high genetic risk without persistent aerobic exercise ( P trend 〈 0.001). Conclusions Both aerobic exercises and NMJ pathway‐related genetic risk were significantly associated with frailty. Persistent aerobic exercises can partly offset NMJ pathway‐related genetic risk to frailty in elderly people.
    Type of Medium: Online Resource
    ISSN: 2190-5991 , 2190-6009
    URL: Issue
    URL: Article
    DOI: 10.1002/jcsm.v12.2
    DOI: 10.1002/jcsm.12658
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2586864-0
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  • 8
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    The tertiary prevention of hepatocellular carcinoma in chronic hepatitis C patients
    Wiley ; 2015
    In:  Journal of Gastroenterology and Hepatology Vol. 30, No. 12 ( 2015-12), p. 1768-1774
    Details
    In: Journal of Gastroenterology and Hepatology, Wiley, Vol. 30, No. 12 ( 2015-12), p. 1768-1774
    Abstract: Pegylated interferon‐alpha plus ribavirin combination (PegIFN/RBV) therapy possesses positive effect in the secondary prevention of hepatocellular carcinoma (HCC) in chronic hepatitis C (CHC) patients. The current study aimed to assess its efficacy in the tertiary prevention and to validate the performance of the MHC class I polypeptide‐related chain A (MICA) level in the prediction of hepatocellular carcinoma (HCC) recurrence. Methods: A multi‐center study enrolling 105 consecutive HCC patients post curative therapies were prospectively recruited. The primary outcome measurement was recurrence of HCC. Results: The mean observational period was 52.7 months (range = 3.9–121.5 months). Fifty‐six (53.3%) patients achieved sustained virological response (SVR). After completion of treatment, 43 (41.0%) patients developed HCC recurrence, and 24 (55.8%) of them had their recurrence within 6 months after completion of therapy. Thirty‐three (76.7%) of the patients with HCC recurrence were of de novo pattern. Those responders tended to have a lower cumulative incidence of recurrence than those non‐responders (43.2 vs 84.8/100 person‐month, log‐rank P  = 0.13). Those non‐responders with a high MICA level ( 〉 100 pg/mL) carried the lowest cancer‐free survival than those non‐responders with a low MICA level and those responders ( P  = 0.002). Cox regression hazard analysis showed high baseline MICA level (Odds ratio [OR] = 4.8, 95% confidence interval [CI]  = 1.1–20.8, P  = 0.04) and a low platelet count ( 〈 100 000/mm 3 ) (OR = 5.4, 95% CI = 1.1–27.0, P  = 0.04) predicted HCC recurrence. Conclusions: PegIFN/RBV therapy carried a limited effect in the tertiary prevention of HCC. A high MICA level predicted HCC recurrence, particularly among those non‐responders.
    Type of Medium: Online Resource
    ISSN: 0815-9319 , 1440-1746
    URL: Issue
    URL: Article
    DOI: 10.1111/jgh.2015.30.issue-12
    DOI: 10.1111/jgh.13012
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 2006782-3
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  • 9
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    Expression analysis and tissue localization of IgZ in the grouper Epinephelus coioides after Vibrio alginolyticus infection and vaccination
    Wiley ; 2021
    In:  Journal of Fish Diseases Vol. 44, No. 10 ( 2021-10), p. 1647-1655
    Details
    In: Journal of Fish Diseases, Wiley, Vol. 44, No. 10 ( 2021-10), p. 1647-1655
    Abstract: The orange‐spotted grouper ( Epinephelus coioides ) is an important marine farmed fish in China. It is affected by the bacterial pathogen Vibrio alginolyticus , which causes high mortality and substantial economic losses. We studied the transcriptional changes of the IgZ gene in E. coioides following V. alginolyticus stimulation and investigated the distribution of IgZ in different tissues. The highest expression level of IgZ occurred in the head kidney. When fish were stimulated with live and inactivated V. alginolyticus , the expression levels of IgZ in the head kidney, spleen, intestine, gills and blood cells were significantly upregulated. In an in situ hybridization study, IgZ mRNA‐positive cells were detected in the head kidney, spleen and gill, but positive signals were not detected in the liver and intestine. IgZ‐labelled cells increased in the head kidney, spleen and gills post‐infection with V. alginolyticus for 21 days. The present study provides additional evidence that IgZ is involved in mucosal immune responses and helps explain the role of IgZ in E .  coioides defence against V .  alginolyticus infection.
    Type of Medium: Online Resource
    ISSN: 0140-7775 , 1365-2761
    URL: Issue
    URL: Article
    DOI: 10.1111/jfd.v44.10
    DOI: 10.1111/jfd.13471
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 432109-1
    detail.hit.zdb_id: 2020444-9
    SSG: 21,3
    SSG: 12
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  • 10
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    Racial and ethnic disparities in untreated patients with hepatitis C virus‐related hepatocellular carcinoma but not in those with sustained virologic response
    Wiley ; 2024
    In:  Alimentary Pharmacology & Therapeutics
    Details
    In: Alimentary Pharmacology & Therapeutics, Wiley
    Abstract: Racial and ethnic disparities exist for hepatitis C virus (HCV) treatment and hepatocellular carcinoma (HCC) survival. Aim To evaluate the impact of HCV treatment on such disparities. Methods In a retrospective cohort study, we analysed 6069 patients with HCV‐related HCC (54.2% Asian, 30.1% White, 8.5% Black, and 7.3% Hispanic) from centres in the United States and Asia. Results The mean age was 61, 60, 59 and 68, respectively, for White, Black, Hispanic and Asian patients. Black patients were most likely to have Barcelona Clinic Liver Cancer stage D, vascular invasion and distant metastasis (23% vs. 5%–15%, 20% vs. 10%–17% and 10% vs. 5%–7%, respectively; all p 〈 0.0001). Treatment rate with direct‐acting antiviral agents (DAA) was 35.9% for Asian, 34.9% for White, 30.3% for Hispanic (30.3%), and 18.7% for Black patients ( p 〈 0.0001). Among those untreated or without sustained virologic response (SVR), 10‐year survival rates were 35.4, 27.5, 19.3 and 14.0, respectively, for Asian, Hispanic, White and Black patients ( p 〈 0.0001). There were no statistically significant differences among those with SVR ( p = 0.44). On multivariable analysis adjusted for relevant confounders, there was no statistically significant association between survival and being Hispanic (aHR: 0.68, p = 0.26) or Black (aHR: 1.18, p = 0.60) versus White. There was a significant association between being Asian American and survival (aHR: 0.24, p = 0.001; non‐U.S. Asian: aHR: 0.66, p = 0.05), and for SVR (aHR: 0.30, p 〈 0.0001). Conclusion DAA treatment rates were suboptimal. Racial and ethnic disparities resolved with HCV cure. Early diagnosis and improved access to HCV treatment is needed for all patients with HCV infection.
    Type of Medium: Online Resource
    ISSN: 0269-2813 , 1365-2036
    URL: Article
    DOI: 10.1111/apt.17863
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 2003094-0
    SSG: 15,3
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