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  • 1
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    Online Resource
    Integrated analysis of transcriptomics to identify hub genes in primary Sjögren's syndrome
    Wiley ; 2022
    In:  Oral Diseases Vol. 28, No. 7 ( 2022-10), p. 1831-1845
    Details
    In: Oral Diseases, Wiley, Vol. 28, No. 7 ( 2022-10), p. 1831-1845
    Abstract: The treatment of patients with primary Sjögren's syndrome is a clinical challenge. Gene expression profile analysis and comprehensive network methods for complex diseases can provide insight into molecular characteristics in the clinical context. Materials and Methods We downloaded gene expression datasets from the Gene Expression Omnibus (GEO) database. We screened differentially expressed genes (DEG) between the pSS patients and the controls by the robust rank aggregation (RRA) method. We explored DEGs’ potential function using gene function annotation and PPI network analysis. Results GSE23117 , GSE40611 , GSE80805 , and GSE127952 were included, including 38 patients and 30 controls. The RRA integrated analysis determined 294 significant DEGs (241 upregulated and 53 downregulated), and the most significant gene aberrantly expressed in SS was CXCL9 ( p  = 6.39E‐15), followed by CXCL13 ( p  = 1.53E‐13). Immune response (GO:0006955; p  = 4.29E‐32) was the most significantly enriched biological process in GO (gene ontology) analysis. KEGG pathway enrichment analysis showed that cytokine–cytokine receptor interaction (hsa04060; p  = 6.46E‐10) and chemokine signaling pathway (hsa04062; p  = 9.54E‐09) were significantly enriched. We defined PTPRC, CD86, and LCP2 as the hub genes based on the PPI results. Conclusion Our integrated analysis identified gene signatures and helped understand molecular changes in pSS.
    Type of Medium: Online Resource
    ISSN: 1354-523X , 1601-0825
    URL: Issue
    URL: Article
    DOI: 10.1111/odi.v28.7
    DOI: 10.1111/odi.13943
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2008428-6
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  • 2
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    Bifunctional Molecular Modification Improving Efficiency and Stability of Inverted Perovskite Solar Cells
    Wiley ; 2018
    In:  Advanced Materials Interfaces Vol. 5, No. 19 ( 2018-10)
    Details
    In: Advanced Materials Interfaces, Wiley, Vol. 5, No. 19 ( 2018-10)
    Abstract: Kinds of interfacial engineering technologies are explored in perovskite solar cells (PSCs), which are proved to be benefit for the devices to achieve higher efficiency, less hysteresis, and/or better stability. In this work, in an inverted p–i–n structured PSC, a monolayer of 5‐aminovaleric acid (5‐AVA) molecules with bifunctional groups is introduced to modify the surface of p‐NiMgLiO compact hole transport layer (HTL). This key surface modification is not only improving the resultant perovskite film's quality, but also facilitating efficient charge extraction at the HTL/perovskite interface, which result in improved power conversion efficiency (PCE) from 18.0 to 19.4%. Furthermore, the 5‐AVA‐modified device can withstand much higher poling bias ( 〉 1.8 V) for a longer time than the pristine one, showing little nonsteady state current and excellent consistency of forward and reverse photocurrent–voltage curves after high bias poling. Benefitting from the exceptionally stable interface, the 5‐AVA‐modified device shows much improved long‐term stability, which can maintain over 90% of its initial PCE after 300 h continuous light soaking at maximum power point tracking condition.
    Type of Medium: Online Resource
    ISSN: 2196-7350 , 2196-7350
    URL: Issue
    URL: Article
    DOI: 10.1002/admi.v5.19
    DOI: 10.1002/admi.201800645
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2750376-8
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  • 3
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    Leuconostoc mesenteroides WHH1141 ameliorates ovalbumin‐induced food allergy in mice
    Wiley ; 2023
    In:  Journal of Food Science
    Details
    In: Journal of Food Science, Wiley
    Abstract: Food allergy (FA) is acknowledged as a significant public health and food safety issue, due to its manifestation as an amplified immune reaction to food antigens. Recently, probiotics within Lactobacillus and Bifidobacterium have been highlighted as a promising strategy against allergic disease by modulating the balance of Th1/Th2 responses. However, the allergy‐alleviating effects of probiotic Leuconostoc mesenteroides strains are unknown. Therefore, this study investigated the potentials of eleven L. mesenteroides strains on the Th1/Th2 balance in vitro by evaluating the expression patterns of interferon‐gamma (IFN‐γ) (Th1 cytokine) and interleukin‐4 (IL‐4) (Th2 cytokine) in mesenteric lymph node‐derived lymphocytes from ovalbumin (OVA)‐sensitized mice. Among strains, WHH1141 incubation caused the highest IFN‐γ/IL‐4 ratio. Oral administration of WHH1141 (1 × 10 9  CFU/mL) in the OVA‐induced FA mouse model for 40 days improved the weight loss and FA pathological symptoms and normalized the serum immunoglobulin E levels. Meanwhile, the OVA‐induced elevated gene expressions of cytokines ( IL‐4 , IL‐5 , and IL‐13 ) and tight‐junction proteins ( ZO‐1 and Occludin ) and levels of cytokines (IL‐4, IL‐5, and IL‐13) and histamine in the jejunum were restored by WHH1141. Furthermore, WHH1141 reversed the reduced gut microbial diversity and short‐chain fatty acid (SCFA) levels, specifically increased Bacteroidota abundance, and decreased Firmicutes abundance in OVA‐induced mice. Overall, these findings suggest that WHH1141 exerts FA‐alleviating effects on OVA‐induced mice, which is involved with the inhibition of the jejunal Th2 immune responses and the modulation of gut microbiome composition and SCFA productions. Practical Application Leuconostoc mesenteroides WHH1141 with FA‐alleviating potentials may be considered a promising approach in the mitigation of FA symptoms.
    Type of Medium: Online Resource
    ISSN: 0022-1147 , 1750-3841
    URL: Article
    DOI: 10.1111/1750-3841.16760
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2006705-7
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  • 4
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    Physalin B inhibits cell proliferation and induces apoptosis in undifferentiated human gastric cancer HGC‐27 cells
    Wiley ; 2022
    In:  Asia-Pacific Journal of Clinical Oncology Vol. 18, No. 3 ( 2022-06), p. 224-231
    Details
    In: Asia-Pacific Journal of Clinical Oncology, Wiley, Vol. 18, No. 3 ( 2022-06), p. 224-231
    Abstract: Physalin B (PB) from Physalis angulata L . (Solanaceae) is a naturally occurring secosteroid with multiple biological activities, including anti‐inflammatory and anticancer activity. However, PB's effects and mechanisms in human gastric cancer (GC) cells are not well characterized. Methods The undifferentiated GC cell line HGC‐27 and semi‐differentiated GC cell line SGC‐7901 were treated with PB. Cell counting kit‐8 (CCK‐8) and colony formation assays were performed to evaluate cell viability. Apoptosis and the cell cycle were assessed by Annexin V/PI and PI/RNase DNA staining assays, respectively, and Western blotting was used to evaluate the expression of a protein. Results PB significantly inhibited the proliferation of HGC‐27 cells in a dose‐ and time‐dependent manner. Moreover, PB induced G0/G1 cycle arrest and caspase‐dependent apoptosis of HGC‐27 cells. Cleaved caspases 8, 3, and 7, poly(ADP)‐ribose polymerase (PARP), and the cyclin‐dependent kinase (CDK) inhibitor p‐Chk2 was induced by PB in HGC‐27 cells, while the cell cycle‐related proteins cyclin D1, cyclin D3, CDK4, CDK6, cyclin E, and phosphorylated retinoblastoma tumor suppressor protein (p‐Rb) were downregulated in a dose‐dependent manner. Conclusions PB inhibits proliferation via cyclin‐dependent kinase and induces caspase‐dependent apoptosis in HGC‐27 cells, suggesting that PB might be a novel and effective agent for undifferentiated GC therapy.
    Type of Medium: Online Resource
    ISSN: 1743-7555 , 1743-7563
    URL: Issue
    URL: Article
    DOI: 10.1111/ajco.v18.3
    DOI: 10.1111/ajco.13593
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2187409-8
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  • 5
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    A novel strategy for tumour therapy combining cell apoptosis and active immunity induced by caspy2, a zebrafish caspase
    Wiley ; 2009
    In:  Journal of Cellular and Molecular Medicine Vol. 13, No. 8b ( 2009-08-02), p. 2271-2281
    Details
    In: Journal of Cellular and Molecular Medicine, Wiley, Vol. 13, No. 8b ( 2009-08-02), p. 2271-2281
    Abstract: Caspy2, a zebrafish protease, is an active caspase for inducing apoptosis in mammalian cells. To investigate whether caspy2‐mediated apoptosis could be used in cancer therapy, its cDNA was amplified and cloned into eukaryotic expression vector pcDNA3.1 + . The recombinant plasmid was mixed with cationic liposome and introduced into various tumour cell lines in vitro . Our data showed that caspy2 induced remarkable apoptosis of cancer cells in vitro . Treatment of mice‐bearing CT26 colon carcinoma or MethA fibrosarcoma with intratumoral injection of liposome‐caspy2 plasmid complex resulted in substantial killing of neoplastic cells and long‐term survivors. Apoptotic cells were widely distributed in caspy2‐treated tumour tissue. Infiltration of CD8 + T lymphocyte was also observed apparently in the tumour tissue after the treatment with caspy2. Tumour‐specific major histocompatibility complex (MHC) class I‐dependent CD8 + cytotoxic T lymphocyte activity was found by means of 51 Cr release assay. In MethA model, the mice acquired a long‐time protective immunity against the parental tumour cell re‐challenge. These results indicated that caspy2 can act as both apoptosis inducer and immune response initiator, which may account for its extraordinary antitumour effect. Furthermore, in vivo depletion of CD8 + T lymphocytes could completely abrogate the antitumour immune activity, whereas the depletion of CD4 + cells showed partial abrogation. In this study, we developed a novel anticancer strategy that combines both induction of apoptosis and immune response in mice‐bearing tumours with a single caspy2 gene. This approach may provide an important way for treatment of cancer.
    Type of Medium: Online Resource
    ISSN: 1582-1838 , 1582-4934
    URL: Issue
    URL: Article
    DOI: 10.1111/jcmm.2009.13.issue-8b
    DOI: 10.1111/j.1582-4934.2009.00693.x
    Language: English
    Publisher: Wiley
    Publication Date: 2009
    detail.hit.zdb_id: 2076114-4
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  • 6
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    A chloroplast envelope membrane protein containing a putative LrgB domain related to the control of bacterial death and lysis is required for chloroplast development in Arabidopsis thaliana
    Wiley ; 2012
    In:  New Phytologist Vol. 193, No. 1 ( 2012-01), p. 81-95
    Details
    In: New Phytologist, Wiley, Vol. 193, No. 1 ( 2012-01), p. 81-95
    Type of Medium: Online Resource
    ISSN: 0028-646X , 1469-8137
    URL: Issue
    URL: Article
    DOI: 10.1111/nph.2011.193.issue-1
    DOI: 10.1111/j.1469-8137.2011.03867.x
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 208885-X
    detail.hit.zdb_id: 1472194-6
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  • 7
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    Characteristic component profiling and identification of different Uncaria species based on high‐performance liquid chromatography–photodiode array detection tandem ion trap and time of flight mass spectrometry coupled with rDNA ITS sequence
    Wiley ; 2018
    In:  Biomedical Chromatography Vol. 32, No. 3 ( 2018-03)
    Details
    In: Biomedical Chromatography, Wiley, Vol. 32, No. 3 ( 2018-03)
    Abstract: Uncaria is a multi‐source herb and its species identification has become a bottleneck in quality control. To study the identification method of different Uncaria species herbs through HPLC–MS coupled with rDNA Internal Transcribed Spacer (rDNA ITS) sequence, both plant morphological traits and molecular identification were used to determine the species of every collected Uncaria herb. The genetic analysis of different Uncaria species was performed using their rDNA ITS sequence as a molecular marker. Meanwhile, the phylogenetic relationships of 22 samples from six Uncaria species were divided and classified clearly. By optimizing the chromatographic conditions, a practical HPLC method to differentiate various varieties of Uncaria herbs was set up based on a set of characteristic components across each species. A high‐performance liquid chromatography–photodiode array detector tandem ion trap and time of flight mass spectrometry technique combined with reference substances was utilized to derive 21 characteristic compounds containing six groups of six Uncaria species in China. Thus, this study provides a feasible method to solve the current problem of confusion in Uncaria species, and makes a significant step forward in the appropriate clinical use, in‐depth research and further utilization of different Uncaria species.
    Type of Medium: Online Resource
    ISSN: 0269-3879 , 1099-0801
    URL: Issue
    URL: Article
    DOI: 10.1002/bmc.v32.3
    DOI: 10.1002/bmc.4119
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 1479945-5
    SSG: 12
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  • 8
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    Selective determination of human immunoglobulin G by flow‐injection chemiluminescence
    Wiley ; 2011
    In:  Luminescence Vol. 26, No. 2 ( 2011-03), p. 142-147
    Details
    In: Luminescence, Wiley, Vol. 26, No. 2 ( 2011-03), p. 142-147
    Abstract: A novel flow‐injection chemiluminescence method was developed for the selective determination of human immunoglobulin G (IgG) in the presence of thiomersal by changing the flow rates of peristaltic pump. The study was based on the independence and additivity of the CL signals of human IgG and thiomersal in the galangin–potassium permanganate–polyphosphoric acid system. In meantime, two equations relating to the concentrations of mixing solutions of human IgG and thiomersal vs the CL intensity were established and solved, on the basis of which the content of thiomersal included in samples was simultaneously determined too. The enhanced CL intensity was in proportion to concerntrations in the range 8.0 × 10 −7 to 8.0 × 10 −5  g/mL for human IgG and 1.0 × 10 −7 to 2.0 × 10 −6  g/mL for thiomersal with the detection limits of 5.0 × 10 −7  g/mL for human IgG and 6.0 × 10 −8  g/mL for thiomersal, respectively. The relative standard deviation for 1.0 × 10 −5  g/mL human IgG was 0.8% and for 2.0 × 10 −7  g/mL thiomersal it was 2.0% ( n = 10). The proposed method was applied to determine three synthetic samples with recoveries of 91.5–109.5%. In addition, the possible chemiluminescence mechanisms are discussed as well. Copyright © 2010 John Wiley & Sons, Ltd.
    Type of Medium: Online Resource
    ISSN: 1522-7235 , 1522-7243
    URL: Issue
    URL: Article
    DOI: 10.1002/bio.v26.2
    DOI: 10.1002/bio.1197
    Language: English
    Publisher: Wiley
    Publication Date: 2011
    detail.hit.zdb_id: 2001819-8
    SSG: 12
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  • 9
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    Oral administration of Bifidobacterium longum WHH2270 ameliorates type 2 diabetes in rats
    Wiley ; 2023
    In:  Journal of Food Science Vol. 88, No. 9 ( 2023-09), p. 3967-3983
    Details
    In: Journal of Food Science, Wiley, Vol. 88, No. 9 ( 2023-09), p. 3967-3983
    Abstract: Accumulating evidence suggests that specific probiotic strains exert hypoglycemic effects on type 2 diabetes mellitus (T2DM), and probiotic strains within Bifidobacterium exhibit potential beneficial effects on T2DM. In this study, α‐glucosidase inhibitory activities of 14 Bifidobacterium strains were assessed in vitro. The hypoglycemic effects of Bifidobacterium longum WHH2270 with high α‐glucosidase inhibitory activity (42.03%) were then investigated in a high‐fat diet/streptozotocin‐induced T2DM rat model. Oral administration of WHH2270 (4 × 10 9 CFU/kg/day) for 8 weeks significantly reversed the reduced body weight and ameliorated the levels of fasting blood glucose, serum triglyceride, serum total cholesterol, glucose tolerance, and insulin resistance in T2DM rats. Using 16S rRNA high‐throughput sequencing of feces, WHH2270 was revealed to reshape the gut microbiome composition by increasing the abundances of Lactobacillus and Bifidobacterium and decreasing the abundances of UCG_005 , Clostridium , and Faecalibacterium in T2DM rats. Besides, the fecal levels of short‐chain fatty acids (SCFAs) including acetate, propionate, and butyrate were also elevated after WHH2270 administration. Moreover, the gene expressions of SCFA receptors FFAR2 and FFAR3 in the colon and pancreas of T2DM rats were restored by WHH2270 administration, accompanied by increased levels of serum acetate. In summary, these results provide evidence that WHH2270 has the potential to improve T2DM symptoms by alleviating hyperglycemia, which was associated with changes in the gut microbiome composition and SCFA production. Practical Application Bifidobacterium longum WHH2270 with high α‐glucosidase inhibitory activity may serve as a promising hypoglycemic agent for the treatment of T2DM.
    Type of Medium: Online Resource
    ISSN: 0022-1147 , 1750-3841
    URL: Issue
    URL: Article
    DOI: 10.1111/jfds.v88.9
    DOI: 10.1111/1750-3841.16727
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2006705-7
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  • 10
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    Lexical learning in a new language leads to neural pattern similarity with word reading in native language
    Wiley ; 2019
    In:  Human Brain Mapping Vol. 40, No. 1 ( 2019-01), p. 98-109
    Details
    In: Human Brain Mapping, Wiley, Vol. 40, No. 1 ( 2019-01), p. 98-109
    Abstract: Previous neuroimaging studies have suggested similar neural activations for word reading in native and second languages. However, such similarities were qualitatively determined (i.e., overlapping activation based on traditional univariate activation analysis). In this study, using representational similarity analysis and an artificial language training paradigm, we quantitatively computed cross‐language neural pattern similarity to examine the modulatory effect of proficiency in the new language. Twenty‐four native Chinese speakers were trained to learn 30 words in a logographic artificial language for 12 days and scanned while performing a semantic decision task after 4‐day training and after 12‐day training. Results showed that higher proficiency in the new language was associated with higher cross‐language pattern similarity in select regions of the reading network.
    Type of Medium: Online Resource
    ISSN: 1065-9471 , 1097-0193
    URL: Issue
    URL: Article
    DOI: 10.1002/hbm.v40.1
    DOI: 10.1002/hbm.24357
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1492703-2
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