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  • 1
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    Online Resource
    The Postembryonic Development of the Immunological Barrier in the Chicken Spleens
    Wiley ; 2019
    In:  Journal of Immunology Research Vol. 2019 ( 2019-05-05), p. 1-10
    Details
    In: Journal of Immunology Research, Wiley, Vol. 2019 ( 2019-05-05), p. 1-10
    Abstract: The avian immune system improves with the development of the lymphoid organs. The chickens’ spleen serves as the largest peripheral lymphoid organ, but little immunological research has been conducted on that spleen during postembryonic development. We investigated the blood-spleen barrier (BSB) by developing morphological architecture, resistance to the corpuscular antigen, immunocyte distribution, gene expression levels of TLR2/4 and cytokines in the spleens of hatched chickens of differing ages. Results demonstrated that the resistance of exogenous carbon particles of the BSB improved with the morphological and structural development of the chicken spleens. The cuboidal endothelial cells which lined the sheathed capillaries were gradually visible, and the discontinuous basement membrane was thickened during postembryonic development. There was an increased number of T and B cells and antigen-presenting cells in the chicken spleen between hatching and adulthood. The mRNA expression levels of TLR2/4, IL-2, IFN- γ , and TNF- α were higher two weeks after hatching, but these decreased and remain stable between 21 and 60 days. As the age increased, the BSB developed structurally and functionally. Our findings provide a better understanding of splenic immune function and the pathogenesis of avian immunology in infectious diseases.
    Type of Medium: Online Resource
    ISSN: 2314-8861 , 2314-7156
    URL: Article
    DOI: 10.1155/2019/6279360
    Language: English
    Publisher: Wiley
    Publication Date: 2019
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  • 2
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    Altered Brain Function in Pediatric Patients With Complete Spinal Cord Injury: A Resting‐State Functional MRI Study
    Wiley ; 2024
    In:  Journal of Magnetic Resonance Imaging Vol. 60, No. 1 ( 2024-07), p. 304-313
    Details
    In: Journal of Magnetic Resonance Imaging, Wiley, Vol. 60, No. 1 ( 2024-07), p. 304-313
    Abstract: Injury to the spinal cord of children may cause potential brain reorganizations, affecting their rehabilitation. However, the specific functional alterations of children after complete spinal cord injury (CSCI) remain unclear. Purpose To explore the specific functional changes in local brain and the relationship with clinical characteristics in pediatric CSCI patients, clarifying the impact of CSCI on brain function in developing children. Study Type Prospective. Subjects Thirty pediatric CSCI patients (7.83 ± 1.206 years) and 30 age‐, gender‐matched healthy children as controls (HCs) (8.77 ± 2.079 years). Field Strength/Sequence 3.0 T/Resting‐state functional MRI ( rs‐fMRI ) using echo‐planar‐imaging ( EPI ) sequence. Assessment Amplitude of low‐frequency fluctuation (ALFF), fractional ALFF (fALFF), and regional homogeneity (ReHo) were used to characterize regional neural function. Statistical Tests Two‐sample t ‐tests were used to compare the ALFF, fALFF, ReHo values of the brain between pediatric CSCI and HCs (voxel‐level FWE correction, P   〈  0.05). Spearman correlation analyses were performed to analyze the associations between the ALFF, fALFF, ReHo values in altered regions and the injury duration, sensory motor scores of pediatric CSCI patients ( P   〈  0.05). Then receiver operating characteristic (ROC) analysis was conducted to identify possible sensitive imaging indicators for clinical therapy. Results Compared with HCs, pediatric CSCI showed significantly decreased ALFF in the right postcentral gyrus (S1), orbitofrontal cortex, and left superior temporal gyrus (STG), increased ALFF in bilateral caudate nucleus, thalamus, middle cingulate gyrus, and cerebellar lobules IV‐VI, and increased ReHo in left cerebellum Crus II and Brodmann area 21. The ALFF value in the right S1 negatively correlated with the pinprick and light touch sensory scores of pediatric CSCI. When the left STG was used as an imaging biomarker for pediatric CSCI, it achieved the highest area under the curve of 0.989. Conclusions These findings may provide potential neural mechanisms for sensory motor and cognitive‐emotional deficits in children after CSCI. Evidence Level 2 Technical Efficacy Stage 5
    Type of Medium: Online Resource
    ISSN: 1053-1807 , 1522-2586
    URL: Issue
    URL: Article
    DOI: 10.1002/jmri.v60.1
    DOI: 10.1002/jmri.29045
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 1146614-5
    detail.hit.zdb_id: 1497154-9
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  • 3
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    Functional Reorganizations Outside the Sensorimotor Regions Following Complete Thoracolumbar Spinal Cord Injury
    Wiley ; 2021
    In:  Journal of Magnetic Resonance Imaging Vol. 54, No. 5 ( 2021-11), p. 1551-1559
    Details
    In: Journal of Magnetic Resonance Imaging, Wiley, Vol. 54, No. 5 ( 2021-11), p. 1551-1559
    Abstract: Studies have shown that loss of sensorimotor function in spinal cord injury (SCI) leads to brain functional reorganization, which may play important roles in motor function recovery. However, the specific functional changes following SCI are still poorly understood. Purpose To investigate whether there are functional reorganizations outside the sensorimotor regions after complete thoracolumbar SCI (CTSCI), and how these reorganizations are associated with clinical manifestations. Study Type Prospective. Subjects Eighteen CTSCI patients (28–67 years of age; 16 men) and 18 age‐, gender‐matched healthy controls (HCs) (27–64 years of age; 16 men). Field Strength/Sequence Resting‐state functional magnetic resonance imaging (RS‐fMRI) using echo‐planar‐imaging (EPI) sequence at 3.0 T. Assessment Data preprocessing was performed using Data Processing Assistant for Resting‐State fMRI (DPARSF). Amplitude of low‐frequency fluctuations (ALFF) was used to characterize regional neural function, and seed‐based functional connectivity (FC) was used to evaluate the functional integration of the brain network. Statistical Tests Two‐sample t ‐tests were used for ALFF and FC measures (the data conform to the normal distribution), partial correlation analysis was used to analyze the correlation between clinical and imaging indicators, and receiver operating characteristic (ROC) analysis was used to search for sensitive imaging indicators. Results Compared with HCs, CTSCI patients showed decreased ALFF in right lingual gyrus (LG), increased ALFF in right middle frontal gyrus (MFG), and decreased FC between the right LG and Vermis_3 (cluster‐level FWE correction with P   〈  0.05). Subsequent correlation analyses revealed that decreased FC between the right LG and Vermis_3 positively correlated with the visual analog scale (VAS) ( P  = 0.043, r  = 0.443). Finally, the ROC analysis showed that the area under the curve (AUC) of FC value between right LG and Vermis3 was 0.881. Data Conclusion These findings suggest a possible theoretical basis of the mechanism of visual‐, emotion‐, and cognition‐related techniques in rehabilitation training for CTSCI.
    Type of Medium: Online Resource
    ISSN: 1053-1807 , 1522-2586
    URL: Issue
    URL: Article
    DOI: 10.1002/jmri.v54.5
    DOI: 10.1002/jmri.27764
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 1146614-5
    detail.hit.zdb_id: 1497154-9
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  • 4
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    The Reorganization of Insular Subregions in Individuals with Below-Level Neuropathic Pain following Incomplete Spinal Cord Injury
    Wiley ; 2020
    In:  Neural Plasticity Vol. 2020 ( 2020-03-10), p. 1-9
    Details
    In: Neural Plasticity, Wiley, Vol. 2020 ( 2020-03-10), p. 1-9
    Abstract: Objective. To investigate the reorganization of insular subregions in individuals suffering from neuropathic pain (NP) after incomplete spinal cord injury (ISCI) and further to disclose the underlying mechanism of NP. Method. The 3D high-resolution T1-weighted structural images and resting-state functional magnetic resonance imaging (rs-fMRI) of all individuals were obtained using a 3.0 Tesla MRI system. A comparative analysis of structure and function connectivity (FC) with insular subareas as seeds in 10 ISCI individuals with below-level NP (ISCI-P), 11 ISCI individuals without NP (ISCI-N), and 25 healthy controls (HCs) was conducted. Associations between the structural and functional alteration of insula subregions and visual analog scale (VAS) scores were analyzed using the Pearson correlation in SPSS 20. Results. Compared with ISCI-N patients, when the left posterior insula as the seed, ISCI-P showed increased FC in right cerebellum VIIb and cerebellum VIII, Brodmann 37 (BA 37). When the left ventral anterior insula as the seed, ISCI-P indicated enhanced FC in right BA18 compared with ISCI-N patients. These increased FCs positively correlated with VAS scores. Relative to HCs, ISCI-P presented increased FC in the left hippocampus when the left dorsal anterior insula was determined as the seed. There was no statistical difference in the volume of insula subregions among the three groups. Conclusion. Our study indicated that distinctive patterns of FC in each subregion of insula suggest that the insular subareas participate in the NP processing through different FC following ISCI. Further, insula subregions could serve as a therapeutic target for NP following ISCI.
    Type of Medium: Online Resource
    ISSN: 2090-5904 , 1687-5443
    URL: Article
    DOI: 10.1155/2020/2796571
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 1454938-4
    detail.hit.zdb_id: 2236872-3
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  • 5
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    Inside Back Cover: Deciphering and Suppressing Over‐Oxidized Nitrogen in Nickel‐Catalyzed Urea Electrolysis (Angew. Chem. Int. Ed. 51/2021)
    Wiley ; 2021
    In:  Angewandte Chemie International Edition Vol. 60, No. 51 ( 2021-12-13), p. 26867-26867
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 60, No. 51 ( 2021-12-13), p. 26867-26867
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v60.51
    DOI: 10.1002/anie.202114404
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
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  • 6
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    Heterozygous Variants in KCNJ10 Cause Paroxysmal Kinesigenic Dyskinesia Via Haploinsufficiency
    Wiley ; 2024
    In:  Annals of Neurology
    Details
    In: Annals of Neurology, Wiley
    Abstract: Most paroxysmal kinesigenic dyskinesia (PKD) cases are hereditary, yet approximately 60% of patients remain genetically undiagnosed. We undertook the present study to uncover the genetic basis for undiagnosed PKD patients. Methods Whole‐exome sequencing was performed for 106 PRRT2 ‐negative PKD probands. The functional impact of the genetic variants was investigated in HEK293T cells and Drosophila . Results Heterozygous variants in KCNJ10 were identified in 11 individuals from 8 unrelated families, which accounted for 7.5% (8/106) of the PRRT2 ‐negative probands. Both co‐segregation of the identified variants and the significantly higher frequency of rare KCNJ10 variants in PKD cases supported impacts from the detected KCNJ10 heterozygous variants on PKD pathogenesis. Moreover, a KCNJ10 mutation‐carrying father from a typical EAST/SeSAME family was identified as a PKD patient. All patients manifested dystonia attacks triggered by sudden movement with a short episodic duration. Patch‐clamp recordings in HEK293T cells revealed apparent reductions in K + currents of the patient‐derived variants, indicating a loss‐of‐function. In Drosophila , milder hyperexcitability phenotypes were observed in heterozygous Irk2 knock‐in flies compared to homozygotes, supporting haploinsufficiency as the mechanism for the detected heterozygous variants. Electrophysiological recordings showed that excitatory neurons in Irk2 haploinsufficiency flies exhibited increased excitability, and glia‐specific complementation with human Kir4.1 rescued the Irk2 mutant phenotypes. Interpretation Our study established haploinsufficiency resulting from heterozygous variants in KCNJ10 can be understood as a previously unrecognized genetic cause for PKD and provided evidence of glial involvement in the pathophysiology of PKD. ANN NEUROL 2024
    Type of Medium: Online Resource
    ISSN: 0364-5134 , 1531-8249
    URL: Article
    DOI: 10.1002/ana.27018
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 80362-5
    detail.hit.zdb_id: 2037912-2
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  • 7
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    The Tissue Distribution of Four Major Coumarins after Oral Administration of Angelicae Pubescentis Radix Extract to Rats Using Ultra-High-Performance Liquid Chromatography
    Wiley ; 2019
    In:  Evidence-Based Complementary and Alternative Medicine Vol. 2019 ( 2019-02-07), p. 1-8
    Details
    In: Evidence-Based Complementary and Alternative Medicine, Wiley, Vol. 2019 ( 2019-02-07), p. 1-8
    Abstract: Angelicae pubescentis radix (APR) is widely applied in treating rheumatoid arthritis in China. Coumarins are the major active compounds of APR extract including columbianetin, columbianetin acetate, osthole, and columbianadin. The in vivo behavior of the four major coumarins of APR has not been systematically reported. A feasible and reliable ultra-performance liquid chromatography (UPLC) method was established and validated for the quantification of the above four coumarins in rat various tissues (including heart, liver, spleen, lung, kidney, uterus, ovary, and muscle) after oral administration of APR extract. The separation was implemented on a Waters ACQUITY BEH C18 column (4.6 mm × 100 mm, 1.7 μ m) with gradient mobile phase comprising acetonitrile-water (with 1mM formic acid) at a flow rate of 0.3 mL/min. The tissue homogenate samples were prepared by liquid-liquid extraction with ethyl acetate. The calibration curves were linear in the range of 1.6-20000 ng/mL for four coumarins with the lower limit of quantitation of 1.6 ng/mL in rat tissues. The intraday and interday precisions and recoveries were all within 80-100% with the relative standard deviations (RSDs) which were all less than 10.9%. The method was successfully applied to the tissue distribution research after oral administration of 6.0 g/kg APR extract to rat. The results revealed that the tissues distributions of four coumarins were in the liver, followed by the ovary, uterus, kidney, lung, heart, spleen, and muscle.
    Type of Medium: Online Resource
    ISSN: 1741-427X , 1741-4288
    URL: Article
    DOI: 10.1155/2019/2365697
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 2171158-6
    detail.hit.zdb_id: 2148302-4
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  • 8
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    Identification of acquired PIGA mutations and additional variants by next‐generation sequencing in paroxysmal nocturnal hemoglobinuria
    Wiley ; 2020
    In:  International Journal of Laboratory Hematology Vol. 42, No. 4 ( 2020-08), p. 473-481
    Details
    In: International Journal of Laboratory Hematology, Wiley, Vol. 42, No. 4 ( 2020-08), p. 473-481
    Abstract: Paroxysmal Nocturnal Hemoglobinuria (PNH) is an acquired clonal disease of hematopoietic stem cells. It is caused by somatic mutation of the X‐linked PIGA gene, resulting in a deficient expression of glycosylphosphatidylinositol‐anchored proteins (GPI‐APs). In this study, we aimed to explore the diagnostic value of next‐generation sequencing (NGS) and potential molecular basis in PNH patients. Methods Genomic DNA of 85 PNH patients was analyzed by a 114‐gene NGS panel. Results Mutational analysis of PIGA identified 124 mutations in 92% PNH patients, including 101 distinct mutations and 23 recurrent mutations. Among them, 102 mutations were newly reported. Most mutations were located in exon 2 of PIGA gene, and truncated mutation was the most common one. Other mutations were detected in 26 out of 85 cases, including five cases of DNMT3A variants, four cases of ASXL1 variants, and four cases of U2AF1 variants. Clonal analysis was performed in one case and outlined a linear evolution pattern in classic PNH. There was a positive correlation between number of PIGA mutations and fraction of GPI‐APs deficient granulocytes. Conclusion The detection of PIGA mutations and additional variants by targeted NGS not only shed light on the genetic characteristics of PNH, but also provided an important reference value in the diagnosis of PNH at molecular level.
    Type of Medium: Online Resource
    ISSN: 1751-5521 , 1751-553X
    URL: Issue
    URL: Article
    DOI: 10.1111/ijlh.v42.4
    DOI: 10.1111/ijlh.13228
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2268600-9
    detail.hit.zdb_id: 2268590-X
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  • 9
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    The influence of guide stabilizers and their application sequences on trueness and precision of surgical guides in free end situations: An in vitro analysis
    Wiley ; 2024
    In:  Clinical Oral Implants Research Vol. 35, No. 3 ( 2024-03), p. 294-304
    Details
    In: Clinical Oral Implants Research, Wiley, Vol. 35, No. 3 ( 2024-03), p. 294-304
    Abstract: To evaluate the impact of guide stabilizers and their application sequences on implant placement accuracy of guided implant surgery in multiple teeth loss at free end. Materials and Methods In this study, 96 implants were placed in the regions of #34, #36, and #37 of 32 identical mandibular models. The influence of using guide stabilizers or not (group A and group B) and various guide stabilizers application sequences (group B: #34 → #36 → #37; group C: #36 → #34 → #37; group D: #37 → #34 → #36) on implant placement trueness and precision was investigated. Data were analyzed using T ‐tests and one‐way ANOVA. Results Group B showed significant benefits in enhancing implant placement precision. Compared to group A, it resulted in reducing 3D‐deviation at crest and 2D deviation in vestibular‐oral direction at both crest and apex. Furthermore, group D demonstrated greater improvement in global implant placement precision by reducing 2D deviation in mesial‐distal direction at both crest and apex. Among the three different stabilizer application sequences, group D exhibited the highest level of implant placement precision. Conclusions In cases of missing teeth at distal free end, the use of guide stabilizers and their application sequences does not have a significant impact on implant placement trueness. However, they do improve implant placement precision compared to methods that do not utilize guide stabilizers. Specifically, applying a guide stabilizer first at the furthest implant site to change teeth loss classification from free end to edentulous space with posterior support is the most reliable sequence.
    Type of Medium: Online Resource
    ISSN: 0905-7161 , 1600-0501
    URL: Issue
    URL: Article
    DOI: 10.1111/clr.v35.3
    DOI: 10.1111/clr.14226
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 1067626-0
    detail.hit.zdb_id: 2027104-9
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  • 10
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    Deciphering and Suppressing Over‐Oxidized Nitrogen in Nickel‐Catalyzed Urea Electrolysis
    Wiley ; 2021
    In:  Angewandte Chemie International Edition Vol. 60, No. 51 ( 2021-12-13), p. 26656-26662
    Details
    In: Angewandte Chemie International Edition, Wiley, Vol. 60, No. 51 ( 2021-12-13), p. 26656-26662
    Abstract: Urea electrolysis is a prospective technology for simultaneous H 2 production and nitrogen suppression in the process of water being used for energy production. Its sustainability is currently founded on innocuous N 2 products; however, we discovered that prevalent nickel‐based catalysts could generally over‐oxidize urea into NO 2 − products with ≈80 % Faradaic efficiencies, posing potential secondary hazards to the environment. Trace amounts of over‐oxidized NO 3 − and N 2 O were also detected. Using 15 N isotopes and urea analogues, we derived a nitrogen‐fate network involving a NO 2 − ‐formation pathway via OH − ‐assisted C−N cleavage and two N 2 ‐formation pathways via intra‐ and intermolecular coupling. DFT calculations confirmed that C−N cleavage is energetically more favorable. Inspired by the mechanism, a polyaniline‐coating strategy was developed to locally enrich urea for increasing N 2 production by a factor of two. These findings provide complementary insights into the nitrogen fate in water–energy nexus systems.
    Type of Medium: Online Resource
    ISSN: 1433-7851 , 1521-3773
    URL: Issue
    URL: Article
    DOI: 10.1002/anie.v60.51
    DOI: 10.1002/anie.202107886
    RVK:
    VA 2100
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2011836-3
    detail.hit.zdb_id: 123227-7
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