In:
Cytometry, Wiley, Vol. 14, No. 4 ( 1993-01), p. 369-376
Abstract:
To identify pig chromosomes in pig‐mouse somatic cell hybrids, dual‐laser flow karyotypes and GTG‐banded metaphase spreads of pig, mouse, and 7 pig‐mouse hybrid cell lines were compared. Pig chromosomes no. 1, 2, 5, 6, 10, 11, 13, 14, 16, 18, X and Y were tentatively assigned to individual peaks in the pig flow karyotype on the basis of DNA content vs. relative chromosome length. In the 7 hybrid cell lines, 7 out of 8 peaks distinct from those of the mouse cell line could be correlated with the presence of pig chromosomes no. 5, 9, 10, 11 or 16, 14, 15, and 18, whereas 1 peak appeared to correspond to the presence of 1 middle‐size chromosome (3, 4, or 7). Other pig chromosomes present in the hybrids could not be detected with certainty due to superposition with mouse peaks and mouse chromosome rearrangements. © 1993 Wiley‐Liss, Inc.
Type of Medium:
Online Resource
ISSN:
0196-4763
,
1097-0320
DOI:
10.1002/cyto.990140405
Language:
English
Publisher:
Wiley
Publication Date:
1993
detail.hit.zdb_id:
2180639-1
detail.hit.zdb_id:
1474272-X
detail.hit.zdb_id:
2180651-2
SSG:
12
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