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  • 1
    In: Physiologia Plantarum, Wiley, Vol. 176, No. 2 ( 2024-03)
    Abstract: The breeding of low phytic acid (LPA) crops is widely considered an effective strategy to improve crop nutrition, but the LPA crops usually have inferior seed germination performance. To clarify the reason for the suboptimal seed performance of LPA rice, this study investigated the impact of reduced seed phytic acid (InsP 6 ) content in rice ins(3)P synthase1 (EC 5.5.1.4, RINO1), one of the key targets for engineering LPA rice, knockouton cellular differentiation in seed embryos and its relation to myo ‐inositol metabolism and auxin signalling during embryogenesis. The results indicated that the homozygotes of RINO1 knockout could initiate differentiation at the early stage of embryogenesis but failed to form normal differentiation of plumule and radicle primordia. The loss of RINO1 function disrupted vesicle trafficking and auxin signalling due to the significantly lowered phosphatidylinositides (PIs) concentration in seed embryos, thereby leading to the defects of seed embryos without the recognizable differentiation of shoot apex meristem (SAM) and radicle apex meristem (RAM) for the homozygotes of RINO1 knockout. The abnormal embryo phenotype of RINO1 homozygotes was partially rescued by exogenous spraying of inositol and indole‐3‐acetic acid (IAA) in rice panicle. Thus, RINO1 is crucial for both seed InsP 6 biosynthesis and embryonic development. The lower phosphatidylinositol (4,5)‐bisphosphate (PI (4,5) P 2 ) concentration and the disorder auxin distribution induced by insufficient inositol supply in seed embryos were among the regulatory switch steps leading to aberrant embryogenesis and failure of seed germination in RINO1 knockout.
    Type of Medium: Online Resource
    ISSN: 0031-9317 , 1399-3054
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 208872-1
    detail.hit.zdb_id: 2020837-6
    SSG: 12
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  • 2
    In: Plant, Cell & Environment, Wiley, Vol. 46, No. 5 ( 2023-05), p. 1453-1471
    Abstract: High temperatures (HT) disturbed the cellular distributions of ABA and ROS contents in rice anthers. The higher ABA in HT‐stressed anthers contributed to HT‐induced pollen abortion by activating ROS generation and oxidation in developing anthers. Interactions between SAPK2 and DEAD‐box ATP‐dependent RNA helicase elF4A‐1 (RH4) were required for ABA‐induced ROS generation during pollen development and its response to HT exposure.
    Type of Medium: Online Resource
    ISSN: 0140-7791 , 1365-3040
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 391893-2
    detail.hit.zdb_id: 2020843-1
    SSG: 12
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  • 3
    In: Agronomy Journal, Wiley, Vol. 112, No. 3 ( 2020-05), p. 1601-1616
    Abstract: The translocation of nonstructural carbohydrates (NSC) from leaf sheaths to filling grains after anthesis contributed greatly to the grain yield of cereal crops. In this study, the effect of nitrogen (N) supply levels on the accumulation and translocation of NSC in leaf sheath tissues and its relationship with the initiation and progression of leaf senescence during grain filling was investigated using two rice ( Oryza sativa L.) genotypes, namely, premature flag leaf senescence mutant ( psf ) and its wild‐type. Three N treatment levels were used to examine N‐supply induced alteration in the activities of several key enzymes involved in NSC translocation and N assimilation in different leaf sheaths. The results show that the NSC translocation rate in leaf sheaths under low nitrogen (LN) treatment was significantly higher than those under normal nitrogen (NN) and high nitrogen (HN) treatments. However, the positive effect of LN on the NSC translocation in leaf sheath was closely associated with its negative effect on grain yield, due to accelerated leaf senescence and shortened leaf longevity. Comparatively, the upper‐positional sheath had a lower NSC amount and higher NSC translocation rate than the lower‐leaf sheaths after heading. High N suppressed sucrose‐phosphate synthase (SPS) activity in leaf sheaths, but enhanced the activity of key enzymes involving in N assimilation in leaf sheaths. The upper sheath had higher activity of sucrose‐metabolizing enzymes and lower activity of N‐assimilating enzymes. Hence, the upper‐leaf sheath had a relatively weak N assimilation and stronger NSC translocation than the lower‐leaf sheaths.
    Type of Medium: Online Resource
    ISSN: 0002-1962 , 1435-0645
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 1471598-3
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  • 4
    In: Clinical Cardiology, Wiley, Vol. 44, No. 10 ( 2021-10), p. 1377-1385
    Abstract: FFR‐guided LCx intervention avoided 79.4% unnecessary LCx intervention with comparable 3‐ year MACE rates between high FFR patients and patients who had low FFR and adequately managed. IVUS‐measured equivalent of low FFR in the LCx include Pre‐PCI LCx MLA and PostPCI LCx MLA, LCx plaque burden, and LM MLA. Treatment of compromised LCx with DEB carried the lowest 3‐year clinical adverse events.
    Type of Medium: Online Resource
    ISSN: 0160-9289 , 1932-8737
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2048223-1
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  • 5
    In: Veterinary Medicine and Science, Wiley, Vol. 7, No. 5 ( 2021-09), p. 1688-1695
    Abstract: Prevalence of brucellosis and MLVA genotyping in animals and humans, isolated from different regions of Pakistan was performed. Animals having history of brucellosis from the field and local farms of Bannu, Mardan, Peshawar, Swat, Lahore and Islamabad were selected for blood collection. Humans that work with them were also selected for sampling in this study. Total of 600 samples were taken from cattle and humans and subjected to Rose Bengal plate Test (RBPT) for the initial screening of positive samples. Designed primers of B.abortus for cattle and B.melitensis for humans were utilised to perform PCR. Culturing and isolation was carried to further to perform MLVA genotyping assay through the selection of two panels of primer markers. Results RBPT showed more number of cases of brucellosis in animals and humans compared to the PCR findings. Genotyping findings based upon MLVA‐15 set of markers demonstrated that the isolated strains of B.abortus fall in the same clade with strain A1, P8 and A2 from Pakistan and also similar with BCCN#02‐45 strain from India. On the other hand, B.melitensis isolated from different districts of Pakistan shared the same clade with BwIM‐AFG 63, BwIM‐AFG 44 strains from Afghanistan and BwIM IRN 37 strain from Iran. Selected VNTR alleles were sequenced for calibration purposes. Conclusion It is concluded that B rucella is prevalent in animals and humans in studied districts of Pakistan. Moreover, A1, P8, BwIM‐AFG 63, BwIM‐AFG 44 and A2 were found the common genotypes in Pakistan.
    Type of Medium: Online Resource
    ISSN: 2053-1095 , 2053-1095
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2819409-3
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  • 6
    In: Applied Organometallic Chemistry, Wiley, Vol. 37, No. 6 ( 2023-06)
    Abstract: Phytoextracts modulated synthesis of nano‐scaled spherical lanthanum oxide nanoparticles (La 2 O 3 NPs) are reported using the aqueous extracts of Hyphaene thebaica as a bioreducing and chelating agent. X‐ray diffraction (XRD) pattern revealed sharp peaks and single‐phase purity. The Debye–Scherrer approximation indicated an average crystallite size of 37 nm, whereas W‐H plot revealed a crystallite size of 59 nm, complemented by the high‐resolution transmission electron microscopy (HR‐TEM). The infrared spectroscopy revealed characteristic La‐O band at 426 and 648 cm −1 . K‐M function revealed the bandgap of 5.6 eV, whereas broad intense emission peaks between 345 and 750 nm. The Raman spectra revealed peaks at 182, 325, 384, and 491 cm −1 . Elemental composition was confirmed using energy‐dispersive X‐ray spectroscopy (EDS) spectrum, and the spotty rings from selected area electron diffraction (SAED) indicated the crystalline nature of the HT‐La 2 O 3 NPs. In addition, the HT‐La 2 O 3 NPs were investigated for in vitro antioxidant, anticancer, antiangiogenic, and cytotoxic potentials. DPPH free radical scavenging activity was highest, that is, 49.54 ± 0.88% at 1000 μg/mL. MTT assay on MCF‐7 and 3T3 cell lines revealed a median lethal concentration of 114 and 43 μg/mL, respectively. The antiangiogenic effect examined by chick chorioallantoic membrane (CAM) assay indicated inhibition of 57.14 ± 0.96% at 1000‐μg/mL concentration with an IC 50 of 551 μg/mL. IC 50 for the brine shrimp lethality assay was 314.2 μg/mL. Our study indicates cytotoxicity of HT‐La 2 O 3 NPs, and it further concluded that HT‐La 2 O 3 NPs were more potent against 3T3 cells as compared with MCF‐7 cells. Further research on the biological synthesis and anticancer studies are recommended for the large‐scale applications of the La 2 O 3 NPs.
    Type of Medium: Online Resource
    ISSN: 0268-2605 , 1099-0739
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1480791-9
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  • 7
    In: Annals of Noninvasive Electrocardiology, Wiley, Vol. 28, No. 2 ( 2023-03)
    Abstract: This systematic review aimed to evaluate the performance of echocardiography‐based programming in comparison with the intracardiac electrocardiogram (IEGM)‐based method for the optimization of cardiac resynchronization therapy (CRT). Methods A literature review was conducted using digital databases to systematically identify the studies reporting CRT optimization through echocardiography compared with IEGM. Detailed patient‐level study characteristics including the type of study, sample size, therapy, the New York Heart Classification (NYHA) status, lead placement, and other parameters were abstracted. Finally, postprogramming outcomes were extracted for each article. Results In a total of 11 studies, 919 patients were recruited for the final analysis. Overall, 692 (75.29%) were males. The mean duration of the QRS complex in our study population ranged from 145.2 ± 21.8 ms to 183 ± 19.9 ms. There was an equal improvement in the NYHA class between the two methods while the left ventricular ejection fraction (LVEF) demonstrated an improvement by IEGM. Many studies supported IEGM to increase the 6‐minute walk test and left ventricular outflow tract velocity time interval (LVOT VTI) when compared to echocardiography. The mean time for echocardiography‐based optimization was 60.15 min while that of IEGM‐based optimization was 6.65 min. Conclusion IEGM is an alternative method for CRT optimization in improving the NYHA class, LVEF, and LVOT VTI, and is less time‐consuming when compared to the echocardiography‐based methods.
    Type of Medium: Online Resource
    ISSN: 1082-720X , 1542-474X
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2111515-1
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  • 8
    In: Reproduction in Domestic Animals, Wiley, Vol. 59, No. 3 ( 2024-03)
    Abstract: Cryopreservation is one of the reliable techniques for long‐term storage of sperm. The success of this technique depends on the choice of cryoprotectant; therefore, a plethora of literature has reported the effects of different cryoprotective agents so far. Kappa‐carrageenan (κ‐carrageenan) is a hydrocolloid polysaccharide extracted from red marine seaweed. Its unique property makes it a promising option as a non‐colligative cryoprotectant. The current study aims to evaluate the cryoprotective effect of k‐carrageenan along with glycerol on ram sperm quality both after equilibration and freezing. Nine Kajli rams were utilized in this experiment for semen collection through an artificial vagina maintained at 42°C. Qualified samples were diluted in tris egg yolk glycerol (TEYG) extender containing different concentrations of k‐carrageenan as 0 mg/mL (control), 0.2, 0.5, 0.8 and 1 mg/mL. Post‐thaw assessment was done at 37°C after 24 h of storage, which showed a significant improvement ( p 〈 .05) in sperm viability, motility, membrane and acrosome integrity in an extender containing k‐carrageenan at a concentration of 0.5 mg/mL compared to control. It is concluded from the current study that the combination of glycerol and 0.5 mg/mL concentration of k‐carrageenan improved the sperm post‐thaw quality.
    Type of Medium: Online Resource
    ISSN: 0936-6768 , 1439-0531
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 2020494-2
    SSG: 12
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  • 9
    In: Microscopy Research and Technique, Wiley, Vol. 82, No. 7 ( 2019-07), p. 1231-1239
    Abstract: For the robust identification of weeds of taxonomically complex families like Poaceae, scanning electron microscopy (SEM) and light microscopy (LM) are of significant implications. In this study, SEM and LM methods were used by investigating 12 Pakistani weedy grass species as an example for foliar epidermal micromorphological characters for its role in the identification of grasses species. Qualitative and quantitative foliar micromorphological variations in stomatal type, number, guard cells, subsidiary cells, silica bodies and trichomes were studied. Significant variation was observed in both qualitative and quantitative features and based on this diversity in epidermal characters, a key was drawn to delimitate the species. Variations in stomata, silica bodies (SiO 2 · n H 2 O), macrohairs, microhairs, hooks, papillae, prickles, and other epidermal structures were used in establishing the taxonomic key. The data were also statistically evaluated by determining its mean, standard deviation, variance, the coefficient of variance, and standard error. Foliar epidermal characters such as silica bodies shape and trichomes were taxonomically more useful than stomatal types, subsidiary, and guard cells. SEM and LM micromorphological characters were of substantial importance in the delimitation of closely related species.
    Type of Medium: Online Resource
    ISSN: 1059-910X , 1097-0029
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2019
    detail.hit.zdb_id: 1474912-9
    SSG: 11
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    Wiley ; 2022
    In:  The International Journal of Health Planning and Management Vol. 37, No. 2 ( 2022-03), p. 1157-1159
    In: The International Journal of Health Planning and Management, Wiley, Vol. 37, No. 2 ( 2022-03), p. 1157-1159
    Abstract: As a developing country, Pakistan reports a high burden of fungal diseases, of which cutaneous mucormycosis remains a prominent infection, presenting as a highly invasive disease with significant mortality. Apart from a high population of at‐risk individuals, multiple factors have precipitated an increment in mucormycosis cases in the country following the COVID‐19 pandemic. These include increased use of corticosteroids, immunosuppression following the viral infection, prolonged stays in the intensive care unit and sub‐optimal laboratory testing available in the country. This article aims to assess the potential implications of a mucormycosis epidemic on a healthcare system already strained under the COVID‐19 pandemic, and provides subsequent recommendations to weather the dual challenge of two deadly pathogens.
    Type of Medium: Online Resource
    ISSN: 0749-6753 , 1099-1751
    URL: Issue
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 1491188-7
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