In:
Journal of Chemical Technology & Biotechnology, Wiley, Vol. 91, No. 4 ( 2016-04), p. 1063-1071
Abstract:
Poly‐3‐hydroxybutyrate ( PHB ) is a polyester that can be accumulated by Azotobacter vinelandii . This polymer is a biodegradable thermoplastic material used for producing plastics for packaging, biobased films, and biocompatible implants. RESULTS PHB produced by wild‐type A . vinelandii and a mutant OP strain were evaluated at different agitation rates. The oxygen transfer rate ( OTR ) evolution was characterized in cultures grown at 300, 400, 500 and 600 rpm, showing for the first time the OTR evolution in cultures of mutant OP . Under the conditions evaluated, the cultures were limited by oxygen. In the cultures grown with the OP strain, lower OTR and specific oxygen uptake rate were obtained, indicating a lower necessity for oxygen for growth in this mutant. A higher amount of PHB can be produced by decreasing the OTR . A maximum PHB of 79% on dry weight was observed in the cultures with the wild‐type strain, whereas the highest PHB productivity (0.18 g L −1 h −1 ) was obtained in cultures of A . vinelandii OP conducted at 600 rpm. CONCLUSION The amount of PHB produced during the period of oxygen limitation can be controlled by the OTR , opening the possibility to evaluate this parameter as a criterion for scaling‐up PHB production by A . vinelandii . © 2015 Society of Chemical Industry
Type of Medium:
Online Resource
ISSN:
0268-2575
,
1097-4660
DOI:
10.1002/jctb.2016.91.issue-4
Language:
English
Publisher:
Wiley
Publication Date:
2016
detail.hit.zdb_id:
1479465-2
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