In:
Pediatric Blood & Cancer, Wiley, Vol. 61, No. 11 ( 2014-11), p. 1949-1954
Abstract:
Wilms tumor (WT) is a pediatric tumor of the kidney, the treatment of which includes heavy chemotherapy. Affected children would likely benefit from more targeted therapies with limited side effects. Establishment of relevant orthotopic WT xenografts is important to better understand mechanisms of WT growth and for preclinical drug testing. Procedure Here we established and characterized orthotopic xenografts from WT cell lines WiT49, CCG‐99‐11, and WT‐CLS1 to ascertain in what aspects each of them recapitulated WT histology, immunophenotype, invasion, and metastatic spread. Results WiT49 xenografts recapitulated near triphasic WTs with clear WT1 staining and anaplastic features, but with tumor restricted to the kidney. On the contrary both CCG‐99‐11 and WT‐CLS1 xenografts conveyed metastatic disease. CCG‐99‐11 showed a blastemal phenotype whereas WT‐CLS1 xenografts did not properly reflect any specific WT subtype. Conclusions From the three tested cell lines, orthotopic WiT49 xenografts best reflect the triphasic pattern of classical WT. Pediatr Blood Cancer 2014;61:1949–1954. © 2014 Wiley Periodicals, Inc.
Type of Medium:
Online Resource
ISSN:
1545-5009
,
1545-5017
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2130978-4
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