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  • 11
    Online Resource
    Online Resource
    miR‐302b regulates cell cycles by targeting CDK 2 via ERK signaling pathway in gastric cancer
    Wiley ; 2016
    In:  Cancer Medicine Vol. 5, No. 9 ( 2016-09), p. 2302-2313
    Details
    In: Cancer Medicine, Wiley, Vol. 5, No. 9 ( 2016-09), p. 2302-2313
    Abstract: To investigate the molecular mechanism of miR‐302b in the regulation of cell proliferation and cell cycle regulation in gastric cancer. Samples of tumor and adjacent normal tissues were collected from 30 gastric cancer patients. Bioinformatics and the dual luciferase report were used for verification of the relationship between miR‐302b expression and cyclin‐dependent kinase 2 ( CDK 2). RT ‐ PCR and western blot were used to examine CDK 2 mRNA and protein levels. The impacts of miR‐302b on CDK 2 expression and extracellular signal‐regulated kinase ( ERK ) signaling pathway were assessed in cells transfected with miR‐302b analogs and CDK 2 overexpression carrier, respectively. We used 3‐(4,5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide (MTT) to assay gastric cancer cell growth after transfection, flow cytometry to analyze cell cycle. Compared with normal tissues, miR‐302b expression was significantly lower in gastric cancer tissues, which was significantly related to lymph node metastasis, metastasis distance, and TNM staging. miR‐302b expression was increased in miR‐302b mimics transfected cells and was significantly decreased in miR‐302b inhibitors transfected cells. CDK 2 is a target gene of miR‐302b. Decreased miR‐302b and increased CDK 2 expressions can significantly promote proliferation and G1/S phase transformation in gastric cancer. miR‐302b promoted the proliferation of gastric cancer cells through upregulation of CDK 2, thereby inhibiting ERK pathway, which can in turn inhibit the promoting ability of miR‐302b on proliferation. The upregulation of miR‐302b reduced the expression of CDK 2, and inhibited ERK signaling pathway, thereby inhibiting cell proliferation and G1/S phase conversion rate. Therefore, miR‐302b provides new perspectives for research of cell regulation and proliferation in gastric cancer, and new targets for gastric cancer diagnosis and treatment.
    Type of Medium: Online Resource
    ISSN: 2045-7634 , 2045-7634
    URL: Issue
    URL: Article
    DOI: 10.1002/cam4.2016.5.issue-9
    DOI: 10.1002/cam4.818
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2659751-2
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  • 12
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    Nanosecond pulsed discharge plasma modified porous polymer adsorbent materials for efficient removal of low‐concentration bisphenol A in liquid
    Wiley ; 2024
    In:  Plasma Processes and Polymers
    Details
    In: Plasma Processes and Polymers, Wiley
    Abstract: The efficient removal of low‐concentration endocrine disruptors is crucial for the protection of the aquatic environment. In this study, porous polymer adsorbent materials were modified by nanosecond pulsed discharge plasma to achieve efficient adsorption of low‐concentration bisphenol A (BPA). The removal efficiency of BPA reached 99% after 10 min of plasma modification at a pulse peak voltage of 28 kV, which increased by 25.8% compared to the raw materials. This enhancement was attributed to the increase of active sites and oxygen‐containing functional groups. The adsorption behaviors of the porous polymer materials were primarily dominated by monolayer chemisorption. Subsequently, comparative experiments further verified the high‐efficiency adsorption performance of porous polymer materials after plasma treatment.
    Type of Medium: Online Resource
    ISSN: 1612-8850 , 1612-8869
    URL: Article
    DOI: 10.1002/ppap.202400021
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 2159694-3
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  • 13
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    Synovial GATA1 mediates rheumatoid arthritis progression via transcriptional activation of NOS2 signaling
    Wiley ; 2018
    In:  Microbiology and Immunology Vol. 62, No. 9 ( 2018-09), p. 594-606
    Details
    In: Microbiology and Immunology, Wiley, Vol. 62, No. 9 ( 2018-09), p. 594-606
    Abstract: Transcriptional regulation of inducible nitric oxide synthase (iNOS) is critically involved in the pathogenesis and progression of rheumatoid arthritis (RA); however, the specific transcription factors that control this process remain largely unidentified. In the present study, it was discovered that expression of the key erythroid factor, globin transcription factor 1 (GATA1), is significantly greater in human RA synovial tissues than in osteoarthritis (OA) tissues. IL 6 was found to induce synovial GATA1 expression in a signal transducer and activator of transcription 3‐dependent manner. Functionally, knockdown of GATA1 expression using specific small interfering RNA treatment was found to compromise immunoreaction‐elicited expression of proinflammatory cytokines and thus impair invasiveness of the human fibroblast‐like synovial cell line MH7A, whereas introduction of exogenous GATA1 was found to promote production of proinflammatory cytokines, leading to greater aggressiveness of MH7A cells. Mechanistically, GATA1 acts as the transcriptional coactivator of NOS2 (the gene encoding iNOS) transcription. Collectively, these data suggest that synovial GATA1 is an essential contributor to development and exacerbation of RA, presumably by inducing NOS2 transcription.
    Type of Medium: Online Resource
    ISSN: 0385-5600 , 1348-0421
    URL: Issue
    URL: Article
    DOI: 10.1111/mim.v62.9
    DOI: 10.1111/1348-0421.12637
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2102145-4
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  • 14
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    Octarepeat peptides of prion are essential for multidrug resistance in gastric cancer cells
    Wiley ; 2012
    In:  Journal of Digestive Diseases Vol. 13, No. 3 ( 2012-03), p. 143-152
    Details
    In: Journal of Digestive Diseases, Wiley, Vol. 13, No. 3 ( 2012-03), p. 143-152
    Abstract: OBJECTIVE:  In previous studies cellular prion protein (PrPc) is confirmed to be involved in multidrug resistance (MDR) of gastric cancer. Although octarepeat peptides are important functional domains of PrPc and are closely related to the transport of Cu 2+ /Zn 2+ and antioxidative function, the significance in MDR remains unknown. We aimed to investigate the role of octarepeat peptides in gastric cancer MDR. METHODS:  Small interfering RNA (siRNA) against PrPc were transfected into adriamycin‐resistant gastric cancer cell lines to inhibit the expression of wild type PrPc, and then constructs encoding PrPc without octarepeat peptides and PrPc without the fifth repeat peptide were transfected, respectively, to establish the cell models. In vitro drug sensitivity, cell apoptosis, measurement of superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px) and glutathione (GSH), as well as changes in glutathione S‐transferase (GST) were detected. RESULTS:  In vitro drug sensitivity test showed that octarepeat peptides could modulate the drug resistance of gastric cancer cells, but the deletion of the fifth repeat peptide had no effect. Specifically, the anti‐apoptotic capacity of gastric cancer cells decreased significantly when the octarepeat peptides of PrPc was absent. Moreover, the activities of total SOD, Cu 2+ /Zn 2+ ‐SOD, GSH‐Px, GSH, and GST detected in different stressing periods revealed that cells lacking octarepeat peptides of PrPc exhibited weakened responses to stress. However, absence of the fifth repeat peptide did not exert any effect on stress response. CONCLUSION:  The octarepeat peptides of prion is responsible for MDR in gastric cancer cells while the fifth repeat peptide is not.
    Type of Medium: Online Resource
    ISSN: 1751-2972 , 1751-2980
    URL: Issue
    URL: Article
    DOI: 10.1111/cdd.2012.13.issue-3
    DOI: 10.1111/j.1751-2980.2011.00563.x
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2317117-0
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  • 15
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    Effect of renal impairment on the pharmacokinetics and safety of dorzagliatin, a novel dual‐acting glucokinase activator
    Wiley ; 2022
    In:  Clinical and Translational Science Vol. 15, No. 2 ( 2022-02), p. 548-557
    Details
    In: Clinical and Translational Science, Wiley, Vol. 15, No. 2 ( 2022-02), p. 548-557
    Abstract: Dorzagliatin is a novel allosteric glucokinase activator targeting both pancreatic and hepatic glucokinase currently under clinical investigation for treatment of type 2 diabetes (T2D). This study aimed to investigate the effect of renal impairment (RI) on dorzagliatin’s pharmacokinetics (PKs) and safety, and to guide appropriate clinical dosing in patients with diabetic kidney disease, including end‐stage renal disease (ESRD). Based on the results from physiologically‐based pharmacokinetic modeling, the predicted outcome of RI on dorzagliatin PK property would be minimum that the plasma exposure area under concentration (AUC) of dorzagliatin in patients with ESRD would increase at about 30% with minimal change in peak concentration (C max ) comparing to those in healthy volunteers (HVs). To definitively confirm the prediction, a two‐part RI study was designed and conducted based on regulatory guidance starting with the patients with ESRD matched with HVs. Results of the RI study showed minimum difference between patients with ESRD and HVs with respect to dorzagliatin exposure with geometric mean ratio of ESRD to HV at 0.81 for C max and 1.11 for AUC. The elimination half‐life, volume of distribution, and systemic clearance for dorzagliatin were similar between the two groups. Dorzagliatin was well‐tolerated in patients with ESRD during the study. Therefore, RI showed no significant impact on dorzagliatin PK, suggesting that dorzagliatin can be readily used in patients with T2D at all stages of RI without need for dose adjustment.
    Type of Medium: Online Resource
    ISSN: 1752-8054 , 1752-8062
    URL: Issue
    URL: Article
    DOI: 10.1111/cts.v15.2
    DOI: 10.1111/cts.13174
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2433157-0
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  • 16
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    FAIRE‐MS reveals mitotic retention of transcriptional regulators on a proteome‐wide scale
    Wiley ; 2023
    In:  The FASEB Journal Vol. 37, No. 2 ( 2023-02)
    Details
    In: The FASEB Journal, Wiley, Vol. 37, No. 2 ( 2023-02)
    Abstract: Mitosis entails global and dramatic alterations, such as higher‐order chromatin organization disruption, concomitant with global transcription downregulation. Cells reliably re‐establishing gene expression patterns upon mitotic exit and maintaining cellular identities remain poorly understood. Previous studies indicated that certain transcription factors (TFs) remain associated with individual loci during mitosis and serve as mitotic bookmarkers. However, it is unclear which regulatory factors remain bound to the compacted mitotic chromosomes. We developed formaldehyde‐assisted isolation of regulatory elements‐coupled mass spectrometry (FAIRE‐MS) that combines FAIRE‐based open chromatin‐associated protein pull‐down and mass spectrometry (MS) to quantify the open chromatin‐associated proteome during the interphase and mitosis. We identified 189 interphase and mitosis maintained (IM) regulatory factors using FAIRE‐MS and found intrinsically disordered proteins and regions (IDP(R)s) are highly enriched, which plays a crucial role in liquid–liquid phase separation (LLPS) and chromatin organization during the cell cycle. Notably, in these IDP(R)s, we identified mitotic bookmarkers, such as CEBPB, HMGB1, and TFAP2A, and several factors, including MAX, HMGB3, hnRNP A2/B1, FUS, hnRNP D, and TIAL1, which are at least partially bound to the mitotic chromosome. Furthermore, it will be essential to study whether these IDP(R)s through LLPS helps cells transit from mitosis to the G1 phase during the cell cycle.
    Type of Medium: Online Resource
    ISSN: 0892-6638 , 1530-6860
    URL: Issue
    URL: Article
    DOI: 10.1096/fsb2.v37.2
    DOI: 10.1096/fj.202201038RRR
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 1468876-1
    SSG: 12
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  • 17
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    Rational Design and Synthesis of 2,2-Bisheterocycle Tandem Derivatives as Non-Nucleoside Hepatitis B Virus Inhibitors
    Wiley ; 2008
    In:  ChemMedChem Vol. 3, No. 9 ( 2008-09-15), p. 1316-1321
    Details
    In: ChemMedChem, Wiley, Vol. 3, No. 9 ( 2008-09-15), p. 1316-1321
    Type of Medium: Online Resource
    ISSN: 1860-7179 , 1860-7187
    URL: Issue
    URL: Article
    DOI: 10.1002/cmdc.v3:9
    DOI: 10.1002/cmdc.200800136
    RVK:
    VA 3569
    Language: English
    Publisher: Wiley
    Publication Date: 2008
    detail.hit.zdb_id: 2209649-8
    SSG: 15,3
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  • 18
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    Studies on Biosynthesis of Chiral Sulfoxides by Using P450 119 Peroxygenase and Its Mutants
    Wiley ; 2022
    In:  ChemistrySelect Vol. 7, No. 47 ( 2022-12-19)
    Details
    In: ChemistrySelect, Wiley, Vol. 7, No. 47 ( 2022-12-19)
    Abstract: Various alkyl‐aryl and heteroaromatic sulfides were catalyzed by P450 119 peroxygenase in the presence of TBHP. P450 119 mutants were used to improve the yield and enantioselectivity of the sulfoxidation. The P450 119 mutation at 153 was found to efficiently improve activity, specificity and enantioselectivity of the sulfoxidation of heteroaromatic sulfides up to 93 % yield and 57 % ee for the first time, meanwhile its thermal stability, kinetic parameters and molecular docking were studied. The experimental results provide a potential method for the biosynthesis of chiral sulfoxides, especially for asymmetric oxidation of heteroaromatic sulfides, by enduring evolution of thermal‐stable P450 119 peroxygenase.
    Type of Medium: Online Resource
    ISSN: 2365-6549 , 2365-6549
    URL: Issue
    URL: Article
    DOI: 10.1002/slct.v7.47
    DOI: 10.1002/slct.202204031
    Language: English
    Publisher: Wiley
    Publication Date: 2022
    detail.hit.zdb_id: 2844262-3
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  • 19
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    Increasing temperature elevates the variation and spatial differentiation of pesticide tolerance in a plant pathogen
    Wiley ; 2021
    In:  Evolutionary Applications Vol. 14, No. 5 ( 2021-05), p. 1274-1285
    Details
    In: Evolutionary Applications, Wiley, Vol. 14, No. 5 ( 2021-05), p. 1274-1285
    Abstract: Climate change and pesticide resistance are two of the most imminent challenges human society is facing today. Knowledge of how the evolution of pesticide resistance may be affected by climate change such as increasing air temperature on the planet is important for agricultural production and ecological sustainability in the future but is lack in scientific literatures reported from empirical research. Here, we used the azoxystrobin‐ Phytophthora infestans interaction in agricultural systems to investigate the contributions of environmental temperature to the evolution of pesticide resistance and infer the impacts of global warming on pesticide efficacy and future agricultural production and ecological sustainability. We achieved this by comparing azoxystrobin sensitivity of 180 P .  infestans isolates sampled from nine geographic locations in China under five temperature schemes ranging from 13 to 25°C. We found that local air temperature contributed greatly to the difference of azoxystrobin tolerance among geographic populations of the pathogen. Both among‐population and within‐population variations in azoxystrobin tolerance increased as experimental temperatures increased. We also found that isolates with higher azoxystrobin tolerance adapted to a broader thermal niche. These results suggest that global warming may enhance the risk of developing pesticide resistance in plant pathogens and highlight the increased challenges of administering pesticides for effective management of plant diseases to support agricultural production and ecological sustainability under future thermal conditions.
    Type of Medium: Online Resource
    ISSN: 1752-4571 , 1752-4571
    URL: Issue
    URL: Article
    DOI: 10.1111/eva.v14.5
    DOI: 10.1111/eva.13197
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 2405496-3
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  • 20
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    The false smut pathogen Ustilaginoidea virens requires rice stamens for false smut ball formation
    Wiley ; 2020
    In:  Environmental Microbiology Vol. 22, No. 2 ( 2020-02), p. 646-659
    Details
    In: Environmental Microbiology, Wiley, Vol. 22, No. 2 ( 2020-02), p. 646-659
    Abstract: Rice false smut has emerged as a serious grain disease in rice production worldwide. The disease is characterized by the transformation of individual rice florets into false smut balls, which is caused by the fungal pathogen Ustilaginoidea virens . To date, little is known about the host factors required for false smut ball formation by U . virens . In this study, we identified histological determinants for the formation of false smut balls by inoculating U . virens into rice floral mutants defective with respect to individual floral parts. The results showed that U . virens could form mature false smut balls in rice floral mutants with defective pistils, but failed to develop false smut balls in the superwoman mutant lacking stamens, identifying that U . virens requires rice stamens to complete its infection cycle. Comparative transcriptome analysis indicated a list of candidate host genes that may facilitate nutrient acquisition by U . virens from the rice stamens, such as SWEET11 , SWEET14 and SUT5 , and genes involved in the biosynthesis of trehalose and raffinose family sugars. These data pinpoint rice stamens as the key target organ of U . virens infection and provide a valuable starting point for dissecting the molecular mechanism of false smut ball formation.
    Type of Medium: Online Resource
    ISSN: 1462-2912 , 1462-2920
    URL: Issue
    URL: Article
    DOI: 10.1111/emi.v22.2
    DOI: 10.1111/1462-2920.14881
    Language: English
    Publisher: Wiley
    Publication Date: 2020
    detail.hit.zdb_id: 2020213-1
    SSG: 12
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