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  • Medicine  (41)
  • 1
    In: Tissue Antigens, Wiley, Vol. 67, No. 4 ( 2006-04), p. 307-317
    Abstract: OX40, a membrane‐bound molecule of the tumor‐necrosis‐factor‐receptor superfamily, is a critical costimulatory receptor during the immune response. Here, we newly generated two specific mouse antihuman OX40 monoclonal antibodies (mAbs) (2G2 and 1F7), whose specificities are quite different from the available OX40 mAb (ACT35) by competition assay. It was also found that both mAbs could enhance the proliferation, activation and differentiation of T lymphocytes primed by anti‐CD3 mAb. These results evidenced that both were functional antihuman OX40 mAbs. Furthermore, stained by 2G2 and 1F7, FCM and immunohistochemistry detected the constitutive expression of OX40 on tumor cell lines from epithelium, breast cancer and glioma tissues. Meanwhile, the non‐tumor tissues (thyroid gland, stomach gland) were also found OX40 expression. These results suggested that OX40 is not only expressed in activated T cells, but also in some tumors as well as normal gland tissues. Such expression pattern indicated that OX40 may be a valuable surface antigen in unveiling its expression and function outside the immune system. Briefly, these novel antibodies may contribute to the evaluation of the mechanism of tumor metastasis and eventually shed light on further study of tumor immunotherapy and autoimmune diseases.
    Type of Medium: Online Resource
    ISSN: 0001-2815 , 1399-0039
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2006
    detail.hit.zdb_id: 1498140-3
    detail.hit.zdb_id: 120440-3
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  • 2
    In: International Endodontic Journal, Wiley, Vol. 45, No. 9 ( 2012-09), p. 807-814
    Abstract: Seo D‐G, Gu Y, Yi Y‐A, Lee S‐J, Jeong J‐S, Lee Y, Chang S‐W, Lee J‐K, Park W, Kim K‐D, Kum K‐Y. A biometric study of C‐shaped root canal systems in mandibular second molars using cone‐beam computed tomography. International Endodontic Journal ,  45 , 807–814, 2012. Abstract Aim  To investigate the configuration of C‐shaped canals in mandibular second molars, canal wall thickness and the orientation of the thinnest area at 1‐mm intervals from the canal orifice to the apex by using cone‐beam computed tomographic (CBCT) images. Methodology  Three‐dimensional CBCT images of 92 Korean mandibular second molars having C‐shaped root canals were analysed to determine their configuration using a modification of Melton’s classification, as well as the thinnest walls and their location. Associations between configuration type and distance from the canal orifice to the apex, as well as associations between the directional orientation of the thinnest root wall and distance from the canal orifice to the apex, were assessed by Fisher’s exact test. Because serial measurements of minimum wall thicknesses were correlated with individual teeth, a mixed‐effects analysis was applied. Results  The most common configuration types were Melton’s type I in the coronal region and Melton’s type III in the apical region. Mean thicknesses of the thinnest root canal walls were 1.39 ± 0.38, 0.85 ± 0.25 and 0.77 ± 0.20 mm in the coronal, middle and apical regions, respectively. The thicker the root canal walls at the orifice region, the greater the decrease in thickness towards the apical region ( P  〈   0.05), with the linguo‐central root area being the thinnest. The pattern of decreasing thickness from the orifice to the apex formed a nonlinear cubic curve. Conclusions  The most prevalent configuration types were Melton’s type I (coronal region) and type III (apical region). The linguo‐central root area was the thinnest in C‐shaped root canals of Korean mandibular second molars. These anatomical variations should be considered during surgical or nonsurgical endodontic procedures.
    Type of Medium: Online Resource
    ISSN: 0143-2885 , 1365-2591
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 603734-3
    detail.hit.zdb_id: 2020354-8
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  • 3
    In: International Endodontic Journal, Wiley, Vol. 47, No. 9 ( 2014-09), p. 896-904
    Abstract: Autotransplantation is a viable treatment option for a missing tooth when there is a suitable donor, especially in adolescents with remaining facial growth. This report presents the aesthetic restoration of a missing maxillary lateral incisor through orthodontic treatment and autotransplantation of a mesiodens using a CBCT ‐fabricated rapid‐prototyping model. Summary A 14‐year‐old male patient with a congenitally missing maxillary lateral incisor was referred from the D epartment of O rthodontics. The teeth were moved orthodontically to regain space for the missing lateral incisor and to close the space of the mesiodens after transplantation. A replica of the donor tooth was fabricated from a cone‐beam computed tomography scan through a rapid‐prototyping machine before autotransplantation surgery. The model was used to create a socket for the graft tooth, thereby shortening the extra‐oral time and minimizing the damage to the root surface. After transplantation and orthodontic tooth movement, the mesiodens was finally restored with an aesthetic laminate restoration. Over 3 years, the aesthetics remained excellent, and the transplant functioned normally without any signs or symptoms of root resorption. Key learning point Missing anterior teeth may be replaced through a combination of orthodontics, autotransplantation with a rapid‐prototyping model and prosthodontic restoration, in growing patients.
    Type of Medium: Online Resource
    ISSN: 0143-2885 , 1365-2591
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 603734-3
    detail.hit.zdb_id: 2020354-8
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  • 4
    In: Allergy, Wiley, Vol. 67, No. 11 ( 2012-11), p. 1430-1436
    Abstract: Hereditary angioedema is a rare autosomal dominant disease, and its correlation between genotype and phenotype seems not to exist. So far, there are very few studies on C hinese population. We aimed to establish a C hinese genetic database of hereditary angioedema and investigated the potential correlation between genotype and phenotype. Method All the eight exons and intron–exon boundaries of C 1 inhibitor gene were detected in 48 unrelated families with HAE . The correlations between genotype and clinical parameters were evaluated by R statistical software. Results Thirty‐five different mutations (25 of them were novel) and 7 SNP s (3 of them were novel) were identified. Significant difference was found in the level of C 1 inhibitor antigen ( P  = 0.01793) between different groups of mutational types. The correlation between different groups of mutational types and the level of C 1 inhibitor antigen (0.5047, P  = 0.00027) was significant. The different groups of mutational types showed neither difference nor correlations of clinical parameters (severity score and the level of C 1 inhibitor function). Conclusion It appears that nonsense, frameshift, and mutations on A rg466 can cause lower level of C 1 inhibitor antigen than missense and in‐frame mutations; however, it does not affect severity of symptoms.
    Type of Medium: Online Resource
    ISSN: 0105-4538 , 1398-9995
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2012
    detail.hit.zdb_id: 2003114-2
    detail.hit.zdb_id: 391933-X
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  • 5
    In: Tissue Antigens, Wiley, Vol. 82, No. 5 ( 2013-11), p. 338-343
    Abstract: The aim of the study was to investigate and compare the clinical manifestations between HLA‐B27 + and HLA‐B27 − ankylosing spondylitis ( AS ) patients in order to obtain knowledge of the impact of HLA‐B27 status on AS , and to inform clinical treatment. A nationwide epidemiological investigation was performed from November 2008 to October 2010. The demographic data and clinical characteristics, and the status of HLA‐B27 were collected using questionnaires and laboratory assay, respectively. A total of 2144 patients (78.5% males and 78.4% HLA‐B27 + AS patients) participated in this study. The percentages of males, patients with family history, and involvement of lumbar spine, thoracic spine and hip joints, were observed to be significantly higher in the HLA‐B27 + AS patients than in their HLA‐B27 − AS peers.
    Type of Medium: Online Resource
    ISSN: 0001-2815 , 1399-0039
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 1498140-3
    detail.hit.zdb_id: 120440-3
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  • 6
    In: Tissue Antigens, Wiley, Vol. 82, No. 6 ( 2013-12), p. 380-386
    Abstract: Human leukocyte antigen ( HLA )‐ B27 is closely associated with ankylosing spondylitis ( AS ). However, the exact correlation between HLA‐B27 subtypes and AS manifestations remains unknown. This study aimed to investigate the correlation between HLA‐B27 polymorphism and the clinical features of AS . This study included 846 patients with AS and 959 healthy controls. Direct sequencing was used to identify the HLA‐B27 genotype. Clinical parameters, including age, age of onset, family history, low back pain, peripheral arthritis, hip joint involvement, dactylitis, uveitis, and sex ratio, were compared among patients with various HLA‐B27 subtypes. In total, 741 AS patients (87.6%) and 39 healthy controls (4%) were HLA‐B27 ‐positive. The most prevalent subtypes were HLA ‐B*2704 (88%) and HLA ‐B*2705 (10.1%) in patients with AS . Compared with HLA ‐B*2704 ‐positive patients, HLA ‐B*2705 ‐positive patients demonstrated a significant increase in the incidence of uveitis (16% vs 6.13%, P  = 0.002) and dactylitis (9.3% vs 3.8%, P  = 0.028) and they had an older age of onset (22.9 ± 8.0 vs 20.7 ± 6.7 years, P  = 0.028). Binary logistic regression analysis revealed that presence of uveitis was significantly associated with HLA ‐B*2705 ( P  = 0.008; odds ratio, 2.63; 95% confidence interval, 1.283–5.393). There were no significant differences in family history, low back pain, peripheral arthritis, or hip joint involvement among HLA‐B27 subtypes. Specific HLA‐B27 subtypes were positively associated with particular clinical features of AS . AS patients with HLA ‐B*2705 demonstrated an older age of onset and had a higher risk of uveitis and dactylitis than did AS patients with HLA ‐B*2704 .
    Type of Medium: Online Resource
    ISSN: 0001-2815 , 1399-0039
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 1498140-3
    detail.hit.zdb_id: 120440-3
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  • 7
    In: Acta Anaesthesiologica Scandinavica, Wiley, Vol. 57, No. 2 ( 2013-02), p. 236-242
    Abstract: Sufentanil is widely used in clinical anaesthesia because of its protective effects against ischaemia/reperfusion injury. Diabetes mellitus elevates the activity of glycogen synthase kinase‐3β ( GSK ‐3β), thereby increasing the permeability of mitochondrial transition pore. This study investigated the role of GSK ‐3β in ameliorating the cardioprotective effect of sufentanil post‐conditioning in diabetic rats. Methods Streptozotocin‐induced diabetic rats and age‐matched non‐diabetic rats were subjected to 30 min of ischaemia and 120 min of reperfusion. Five minutes before reperfusion, rats were administered one of the following: a vehicle, sufentanil (1 μg/kg), or a GSK ‐3β inhibitor SB 216763 (0.6 mg/kg). Myocardial infarct size, cardiac troponin I , and the activity of GSK ‐3β were then assessed. Results Sufentanil post‐conditioning significantly reduced myocardial infarct size in the non‐diabetic, but not in diabetic rats. SB216763 reduced infarct size in both diabetic and non‐diabetic animals. Sufentanil‐induced phospho‐ GSK ‐3β was reduced 5 min after reperfusion in diabetic rats, but not in non‐diabetic rats. Conclusions Sufentanil treatment was ineffective in preventing against ischaemia/reperfusion in diabetic rats, which is associated with the activation of GSK ‐3β. Our results also suggest that direct inhibition of GSK ‐3β may provide a strategy to protect diabetic hearts against ischaemia/reperfusion injury.
    Type of Medium: Online Resource
    ISSN: 0001-5172 , 1399-6576
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2013
    detail.hit.zdb_id: 80002-8
    detail.hit.zdb_id: 2004319-3
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  • 8
    In: International Endodontic Journal, Wiley, Vol. 48, No. 4 ( 2015-04), p. 380-389
    Abstract: To obtain concurrent radicular measurements in the mesiobuccal ( MB ) and mesiolingual ( ML ) canals of mandibular first molars using scanned data of micro‐computed tomography (μ CT ) with novel software. Methodology The scanned data from 37 mandibular first molar mesial roots were reconstructed and analysed with custom‐developed software (Kappa2). For each canal, three‐dimensional (3D) surface models were re‐sliced at 0.1‐mm intervals perpendicular to the central axis. Dentine thicknesses, canal widths and 3D curvatures were measured automatically on each slice. Measurements were analysed statistically with anova for differences at each direction and at different levels of both canals. Results Lateral dentine thicknesses were significantly higher than mesial and distal thicknesses, at all the levels of both canals ( P  〈   0.001). Mesial thicknesses were significantly higher than distal thicknesses in the coronal third of both canals ( P  〈   0.001). Thinnest dentine thicknesses were mainly located on the disto‐inside of both canals. Narrowest canal widths were 0.24 ± 0.10 and 0.22 ± 0.09 mm in MB and ML canals, respectively. Canal curvatures were greatest in the apical third of both canals ( P  〈   0.001), and they were greater in the MB canals than in the ML canals ( P  〈   0.05). Conclusions Micro‐computed tomography with novel software provided valuable anatomical information for optimizing instrumentation and minimizing mishaps in nonsurgical root canal treatment.
    Type of Medium: Online Resource
    ISSN: 0143-2885 , 1365-2591
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2015
    detail.hit.zdb_id: 603734-3
    detail.hit.zdb_id: 2020354-8
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  • 9
    In: Allergy, Wiley, Vol. 69, No. 3 ( 2014-03), p. 359-364
    Type of Medium: Online Resource
    ISSN: 0105-4538
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2014
    detail.hit.zdb_id: 391933-X
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  • 10
    Online Resource
    Online Resource
    Wiley ; 2016
    In:  Scandinavian Journal of Immunology Vol. 83, No. 3 ( 2016-03), p. 203-211
    In: Scandinavian Journal of Immunology, Wiley, Vol. 83, No. 3 ( 2016-03), p. 203-211
    Abstract: The acute respiratory distress syndrome ( ARDS ), a clinical complication of severe acute lung injury ( ALI ) in humans, is a leading cause of morbidity and mortality in critically ill patients. Here, we explored the association between IL ‐17 and development of ALI using LPS ‐induced murine model. We found that IL ‐17 level was elevated in bronchoalveolar lavage ( BAL ) fluid of ALI mice. Upregulation of IL ‐17 resulted in increased severity of ALI as evidenced by decreased body weight and survival rate, elevated level of total protein and albumin in BAL fluid, as well as more apparent histopathology changes of lung. Induction of ALI was impaired in IL ‐17‐deficient mice. Management of IL ‐17 could modulate LPS ‐induced pulmonary inflammation, as reflected by the total cell and neutrophil counts, proinflammatory cytokines, as well as chemokines in BAL fluid. Of note, blockade of IL ‐17 effectively inhibited the lung inflammation and alleviated ALI severity. Finally, we confirmed the clinical relevance and found that IL ‐17 expression was elevated and associated with the disease severity in patients with ARDS . In essence, IL ‐17 was crucial for development of ALI , suggesting a potential application for IL ‐17‐based therapy in clinical practice.
    Type of Medium: Online Resource
    ISSN: 0300-9475 , 1365-3083
    URL: Issue
    RVK:
    Language: English
    Publisher: Wiley
    Publication Date: 2016
    detail.hit.zdb_id: 2020954-X
    detail.hit.zdb_id: 120476-2
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