In:
Physiologia Plantarum, Wiley, Vol. 135, No. 4 ( 2009-04), p. 426-435
Abstract:
Recently, we have isolated salt‐tolerance genes (SATs) on the basis of the overexpression screening of yeast with a maize cDNA library from kernels. One of the selected genes [ salt‐tolerance 32 ( SAT32 )] appears to be a key determinant for salt stress tolerance in yeast cells. Maize SAT32 cDNA encodes for a 49‐kDa protein, which is 41% identity with the Arabidopsis salt‐tolerance 32 ( AtSAT32 ) unknown gene. Arabidopsis Transfer‐DNA (T‐DNA) knockout AtSAT32 ( atsat32 ) altered root elongation, including reduced silique length and reduced seed number. In an effort to further assess salinity tolerance in Arabidopsis , we have functionally characterized the AtSAT32 gene and determined that salinity and the plant hormone ABA induced the expression of AtSAT32 . The atsat32 mutant was more sensitive to salinity than the wild‐type plant. On the contrary, Arabidopsis overexpressing AtSAT32 (35S:: AtSAT32 ) showed enhanced salt tolerance and increased activity of vacuolar H + ‐pyrophosphatase (V‐PPase, EC 3.6.1.1) under high‐salt conditions. Consistent with these observations, 35S:: AtSAT32 plants exhibited increased expression of salt‐responsive and ABA‐responsive genes, including the Rd29A , Erd15 , Rd29B , Rd22 and RAB18 genes. Therefore, our results indicate that AtSAT32 is involved in both salinity tolerance and ABA signaling as a positive regulator in Arabidopsis .
Type of Medium:
Online Resource
ISSN:
0031-9317
,
1399-3054
DOI:
10.1111/ppl.2009.135.issue-4
DOI:
10.1111/j.1399-3054.2008.01202.x
Language:
English
Publisher:
Wiley
Publication Date:
2009
detail.hit.zdb_id:
208872-1
detail.hit.zdb_id:
2020837-6
SSG:
12
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