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  • Walter de Gruyter GmbH  (2)
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  • Walter de Gruyter GmbH  (2)
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  • 1
    Online Resource
    Online Resource
    Walter de Gruyter GmbH ; 1998
    In:  Zeitschrift für Naturforschung C Vol. 53, No. 1-2 ( 1998-2-1), p. 93-100
    In: Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 53, No. 1-2 ( 1998-2-1), p. 93-100
    Abstract: The photoprotective function of the ketocarotenoid astaxanthin in Haematococcus was questioned. When exposed to high irradiance and/or nutritional stress, green Haematococcus cells turned red due to accumulation of an immense quantity of the red pigment astaxanthin. Our results demonstrate that: 1) The addition of diphenylamine, an inhibitor of astaxanthin biosynthesis, causes cell death under high light intensity; 2) Red cells are susceptible to high light stress to the same extent or even higher then green ones upon exposure to a very high light intensity (4000 μmol photon m -2 s -1 ); 3) Addition of 1 O 2 generators (methylene blue, rose bengal) under noninductive conditions (low light of 100 (μmol photon m -2 s -1 ) induced astaxanthin accumulation. This can be reversed by an exogenous 1 O 2 quencher (histidine); 4) Histidine can prevent the accumulation of astaxanthin induced by phosphate starvation. We suggest that: 1) Astaxanthin is the result of the photoprotection process rather than the protective agent; 2) 1 O 2 is involved indirectly in astaxanthin accumulation process.
    Type of Medium: Online Resource
    ISSN: 1865-7125 , 0939-5075
    RVK:
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 1998
    detail.hit.zdb_id: 2078107-6
    SSG: 12
    Location Call Number Limitation Availability
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  • 2
    Online Resource
    Online Resource
    Walter de Gruyter GmbH ; 1999
    In:  Zeitschrift für Naturforschung C Vol. 54, No. 1-2 ( 1999-2-1), p. 49-54
    In: Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 54, No. 1-2 ( 1999-2-1), p. 49-54
    Abstract: The addition of 2.5 mᴍ glufosinate ammonium (BASTA), a well known plant killer, to Haematococcus pluvialis culture efficiently inhibits cell growth, blocks the activity of glutamine synthetase (GS) and induces astaxanthin accumulation. Conversely, methionine-S-sulfoximine (MSX), a well known GS inhibitor, had no effect on neither these parameters. When GS activity was tested in vitro, MSX inhibited the activity at high concentrations (mᴍ), while glufosinate was effective in the μm range. We have found that in the presence of glufosinate, ammonia is excreted from the cells. Therefore, we suggest that this process enables Haematococcus cells to escape the potentially harmful effect of glufosinate. As a consequence of the inability to assimilate nitrogen, astaxanthin is accumulated. This situation resembles the response of Haematococcus cells to nitrogen starvation
    Type of Medium: Online Resource
    ISSN: 1865-7125 , 0939-5075
    RVK:
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 1999
    detail.hit.zdb_id: 2078107-6
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
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