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  • Walter de Gruyter GmbH  (2)
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  • Walter de Gruyter GmbH  (2)
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  • 1
    Online Resource
    Online Resource
    Phosphorylation of Pit-1 by cyclin-dependent kinase 5 at serine 126 is associated with cell proliferation and poor prognosis in prolactinomas
    Walter de Gruyter GmbH ; 2021
    In:  Open Chemistry Vol. 19, No. 1 ( 2021-07-09), p. 785-793
    Details
    In: Open Chemistry, Walter de Gruyter GmbH, Vol. 19, No. 1 ( 2021-07-09), p. 785-793
    Abstract: Pit-1 (POU1F1) is a POU-homeodomain transcription factor, and it is one of the most important tissue-specific transcription factors in pituitary development. Cyclin-dependent kinase 5 (CDK5) is a protein kinase that can phosphorylate many key transcription factors, but the mechanism under which CDK5 phosphorylates Pit-1 is unclear. To investigate whether CDK5 can regulate cell proliferation and promote hormone secretion through phosphorylation of Ser126-Pit-1 in prolactinomas, we generated an antibody that specifically recognizes phosphorylated serine at position 126 of Pit-1 (Ser126-Pit-1). We used western blotting to detect the level of Pit-1 phosphorylation and observed the proliferation and apoptosis of GH3 cells with different levels of Pit-1 phosphorylation by clone formation experiments, cell viability assays, and flow cytometry. ELISA was used to measure the level of PRL in the supernatant of GH3 cells. Tissue microarrays and immunohistochemistry were used to evaluate the expression of the phosphorylation level of Ser126-Pit-1 (pSer126-Pit-1) in prolactinomas. Our data indicated that Ser126-Pit-1 is specifically phosphorylated by CDK5 and high-level pSer-126-Pit-1 can promote cell proliferation and PRL secretion. In addition, a higher level of pSer-126-Pit-1 correlates with a worse prognosis in patients with prolactinoma. Our results show that CDK5 mediated Ser126-Pit-1 phosphorylation and regulated prolactinoma progression and PRL secretion.
    Type of Medium: Online Resource
    ISSN: 2391-5420
    URL: Article
    DOI: 10.1515/chem-2021-0070
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2021
    detail.hit.zdb_id: 2825411-9
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  • 2
    Online Resource
    Online Resource
    Is it necessary for all samples to quantify 25OHD 2 and 25OHD 3 using LC-MS/MS in clinical practice?
    Walter de Gruyter GmbH ; 2018
    In:  Clinical Chemistry and Laboratory Medicine (CCLM) Vol. 56, No. 2 ( 2018-01-26), p. 273-277
    Details
    In: Clinical Chemistry and Laboratory Medicine (CCLM), Walter de Gruyter GmbH, Vol. 56, No. 2 ( 2018-01-26), p. 273-277
    Abstract: The demand for vitamin D testing is increasing in China. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) immunoassay is specific and accurate but requires expensive equipment, experienced operators, and complicated pretreatment of serum. Automated immunoassays are simple and convenient but only determine total 25-hydroxyvitamin D (25OHD). The objective of this study was to quantify 25OHD 2 and 25OHD 3 in patients to assist clinical physicians and laboratory directors in choosing the most appropriate method to determine 25OHD. Methods: Vitamin D testing was conducted for 23,695 patients in Peking Union Medical College Hospital from May 2015 to January 2017. Using this large data set, the prevalence and levels of 25OHD 2 were analyzed. LC-MS/MS was used to separately determine 25OHD 2 and 25OHD 3 . Results: 25OHD 2 (≥2.5 ng/mL) was detected in 16.4% (3877/23,695) of patients. Males had a significantly lower incidence of detectable 25OHD 2 (p 〈 0.01); 1077 (13.9%) samples contained detectable 25OHD 2 (median: 3.7 ng/mL; 2.5%–97.5%: 2.5–17.2 ng/mL). For females, 2800 (17.5%) samples contained detectable 25OHD 2 (median: 4.0 ng/mL; range: 2.5–20.6 ng/mL). Of the 3877 patients with detectable 25OHD 2 , males had a significantly higher level of 25OHD 3 (p 〈 0.01). There was no significant difference in total 25OHD. The proportion of 25OHD 2 in total 25OHD was 1.3%–100%; 87.5% (3391/3877) of the samples contained 〈 10 ng/mL 25OHD 2 . 25OHD 2 negatively correlated with 25OHD 3 (r=−0.197, p 〈 0.01) and positively correlated with total 25OHD (r=0.217, p 〈 0.01). Conclusions: Prevalence of 25OHD 2 in patients tested for vitamin D is relatively high in China. 25OHD 2 is significantly negatively correlated with 25OHD 3 .
    Type of Medium: Online Resource
    ISSN: 1437-4331 , 1434-6621
    URL: Article
    DOI: 10.1515/cclm-2017-0520
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2018
    detail.hit.zdb_id: 1418007-8
    detail.hit.zdb_id: 1492732-9
    SSG: 15,3
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