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  • 1
    Online Resource
    Online Resource
    Potentiation of Bleomycin Lethality in HeLa and V79 Cells by Bee Venom
    Walter de Gruyter GmbH ; 2009
    In:  Archives of Industrial Hygiene and Toxicology Vol. 60, No. 3 ( 2009-09-1), p. 317-326
    Details
    In: Archives of Industrial Hygiene and Toxicology, Walter de Gruyter GmbH, Vol. 60, No. 3 ( 2009-09-1), p. 317-326
    Abstract: This study investigated possible growth-inhibiting effects of bee venom applied alone or in combination with a cytotoxic drug bleomycin on HeLa and V79 cells in vitro based on clone formation, cell counting, and apoptosis. Melittin, the key component of bee venom, is a potent inhibitor of calmodulin activity, and also a potent inhibitor cell growth and clonogenicity. Intracellular accumulation of melittin correlates with the cytotoxicity of antitumour agents. Previous studies indicated that some calcium antagonists and calmodulin inhibitors enhanced intracellular levels of antitumor agents by inhibiting their outward transport. In this study, treatment of exponentially growing HeLa and V79 cells with bleomycin caused a dose-dependent decrease in cell survival due to DNA damage. This lethal effect was potentiated by adding a non-lethal dose of the bee venom. By preventing repair of damaged DNA, bee venom inhibited recovery from potentially lethal damage induced by bleomycin in V79 and HeLa cells. Apoptosis, necrosis, and lysis were presumed as possible mechanisms by which bee venom inhibited growth and clonogenicity of V79 cells. HeLa cells, on the other hand, showed greater resistance to bee venom. Our findings suggest that bee venom might find a therapeutic use in enhancing cytotoxicity of antitumour agent bleomycin.
    Type of Medium: Online Resource
    ISSN: 0004-1254
    URL: Article
    DOI: 10.2478/10004-1254-60-2009-1936
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2009
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  • 2
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    Online Resource
    Naringenin ameliorates pathological changes in liver and kidney of diabetic mice: a preliminary study / Naringenin reducira histopatološke promjene u jetri i bubregu miševa s dijabetesom
    Walter de Gruyter GmbH ; 2016
    In:  Archives of Industrial Hygiene and Toxicology Vol. 67, No. 1 ( 2016-3-1), p. 19-24
    Details
    In: Archives of Industrial Hygiene and Toxicology, Walter de Gruyter GmbH, Vol. 67, No. 1 ( 2016-3-1), p. 19-24
    Abstract: The effect of naringenin, a flavonoid found in grapefruit, orange, and tomato, on lipid peroxidation and histopathological changes in the liver and kidneys of alloxan-induced diabetic mice were investigated. Two days after alloxan injection (75 mg kg −1 , i.v.), naringenin ethanolic solution (0.5 % v/v) was given to mice intraperitoneally (50 mg kg −1 per day) for seven days. Naringenin’s impact on lipid peroxidation was measured by the 2-thiobarbituric acid test and histopathological changes were examined under a light microscope. Naringenin administration resulted in a significant decrease of lipid peroxidation level in liver and kidney tissue, as well as in a decreased number of vacuolated liver cells and degree of vacuolisation. Indications of tissue repair in kidney suggested that amelioration of diabetes-induced renal damage could be achieved over a longer period of time. Findings suggest that naringenin could be considered a dietary supplement in the prevention or treatment of diabetic complications and other diseases connected with oxidative stress, and gives a hope that it could show similar effects in the treatment of diabetes in humans.
    Type of Medium: Online Resource
    ISSN: 0004-1254
    URL: Article
    DOI: 10.1515/aiht-2016-67-2708
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2016
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  • 3
    Online Resource
    Online Resource
    Different damaging effects of volatile anaesthetics alone or in combination with 1 and 2 Gy gamma-irradiation in vivo on mouse liver DNA: a preliminary study
    Walter de Gruyter GmbH ; 2023
    In:  Archives of Industrial Hygiene and Toxicology Vol. 74, No. 1 ( 2023-03-01), p. 22-33
    Details
    In: Archives of Industrial Hygiene and Toxicology, Walter de Gruyter GmbH, Vol. 74, No. 1 ( 2023-03-01), p. 22-33
    Abstract: As the number of radiotherapy and radiology diagnostic procedures increases from year to year, so does the use of general volatile anaesthesia (VA). Although considered safe, VA exposure can cause different adverse effects and, in combination with ionising radiation (IR), can also cause synergistic effects. However, little is known about DNA damage incurred by this combination at doses applied in a single radiotherapy treatment. To learn more about it, we assessed DNA damage and repair response in the liver tissue of Swiss albino male mice following exposure to isoflurane (I), sevoflurane (S), or halothane (H) alone or in combination with 1 or 2 Gy irradiation using the comet assay. Samples were taken immediately (0 h) and 2, 6, and 24 h after exposure. Compared to control, the highest DNA damage was found in mice receiving halothane alone or in combination with 1 or 2 Gy IR treatments. Sevoflurane and isoflurane displayed protective effects against 1 Gy IR, while with 2 Gy IR the first adverse effects appeared at 24 h post-exposure. Although VA effects depend on liver metabolism, the detection of unrepaired DNA damage 24 h after combined exposure with 2 Gy IR indicates that we need to look further into the combined effects of VA and IR on genome stability and include a longer time frame than 24 h for single exposure as well as repeated exposure as a more realistic scenario in radiotherapy treatment.
    Type of Medium: Online Resource
    ISSN: 1848-6312
    URL: Article
    DOI: 10.2478/aiht-2023-74-3692
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2023
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  • 4
    Online Resource
    Online Resource
    UČINKOVITOST BIOLOŠKI AKTIVNIH SASTAVNICA MATIČNE MLIJEČI: ANALIZA I STANDARDIZACIJA
    Walter de Gruyter GmbH ; 2013
    In:  Archives of Industrial Hygiene and Toxicology Vol. 64, No. 3 ( 2013-09-1), p. 445-461
    Details
    In: Archives of Industrial Hygiene and Toxicology, Walter de Gruyter GmbH, Vol. 64, No. 3 ( 2013-09-1), p. 445-461
    Abstract: Funkcionalna hrana postala je važan sektor u promicanju zdravstvenih prednosti hrane preko njenih funkcionalnih sastojaka sadržanih u ovim proizvodima. Posljednjih godina povećano je zanimanje potrošača i prehrambene industrije ne samo za funkcionalne sastojke hrane nego i za način na koji mogu pomoći u održavanju ljudskoga zdravlja. Mišljenje da prehrana ima važnu ulogu u sprečavanju i liječenju bolesti široko je prihvaćeno. Brojni literaturni podatci upućuju na to da pčelinji proizvodi, uključujući matičnu mliječ, mogu imati blagotvoran učinak na ljudsko zdravlje. Matična je mliječ pčelinji proizvod s brojnim biološki aktivnim sastojcima zdrave hrane. Izlučevina je posebnih žlijezda (hipofaringealnih i mandibularnih) koju pčele radilice (Apis mellifera L.) koriste za hranjenje matice i ličinki. Matična mliječ sadrži znatnu količinu proteina, slobodnih aminokiselina, lipida, vitamina i šećera. Njene su karakteristične sastavnice bioaktivne tvari, primjerice trans-10-hidroksi-2-decenska kiselina, antibakterijski proteini, i protein mase 350 kDa protein, nazvan apisin, koji stimulira proliferaciju ljudskih monocita. Matična mliječ pokazuje brojne fiziološke i farmakološke učinke u sisavaca, uključujući vazodilataciju i sniženje krvnog tlaka, sniženje vrijednosti kolesterola, usporavanje procesa starenja, antioksidativnu aktivnost, imunomodulacijski te protuupalni, antimikrobni, estrogenski, antiosteoporozni i protutumorski učinak. Ublažavanje simptoma menopauze također je jedan od povoljnih učinaka matične mliječi. Ovaj pregledni članak upućuje na molekularne mehanizme uključene u razvoj pojedinih poremećaja te na odnos između matične mliječi i raznovrsnih bolesti na temelju dosadašnjih rezultata različitih in vivo i in vitro istraživanja. Rad također opisuje tumačenja mogućih mehanizama višestrukih korisnih učinaka matične mliječi u borbi protiv starenja i komplikacija povezanih sa starenjem. Osim toga, navode se i preporuke o definiranju standarda kakvoće matične mliječi s obzirom na to da rezultati analize ovoga pčelinjega proizvoda upućuju na značajne razlike u sastavu i djelotvornosti svježih i dehidriranih uzoraka.
    Type of Medium: Online Resource
    ISSN: 0004-1254
    URL: Article
    DOI: 10.2478/10004-1254-64-2013-2332
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2013
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  • 5
    Online Resource
    Online Resource
    Sevoflurane and isoflurane genotoxicity in kidney cells of mice
    Walter de Gruyter GmbH ; 2017
    In:  Archives of Industrial Hygiene and Toxicology Vol. 68, No. 3 ( 2017-9-26), p. 228-235
    Details
    In: Archives of Industrial Hygiene and Toxicology, Walter de Gruyter GmbH, Vol. 68, No. 3 ( 2017-9-26), p. 228-235
    Abstract: The aim of this study was to evaluate the DNA damage and repair in kidney cells of Swiss albino mice after repeated exposure to sevoflurane and isoflurane and compare their detrimental effects. We used the alkaline comet assay to establish the genetic damage and measured three parameters: tail length, tail moment, and tail intensity of comets. These parameters were measured immediately after exposure to the above mentioned inhalation anaesthetics, two hours, six hours, and 24 hours later and were compared with the control group. Mean values of all three parameters were significantly higher in experimental groups compared to the control group. DNA damage in kidney cells of mice exposed to sevoflurane increased continuously before it reached its peak 24 hours after exposure. Isoflurane induced the highest DNA damage two hours after exposure. Levels of DNA damage recorded 24 h after cessation of exposure to both tested compounds suggest that sevoflurane was slightly more genotoxic than isoflurane to kidney cells of mice. According to these results, the currently used volatile anaesthetics sevoflurane and isoflurane are able to damage DNA in kidney cells of mice. Such findings suggest a possibility for similar outcomes in humans and that fact must be taken into account in everyday clinical practice.
    Type of Medium: Online Resource
    ISSN: 0004-1254
    URL: Article
    DOI: 10.1515/aiht-2017-68-2941
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2017
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  • 6
    Online Resource
    Online Resource
    Kidney cell DNA damage caused by combined exposure to volatile anaesthetics and 1 Gy or 2 Gy radiotherapy dose in vivo
    Walter de Gruyter GmbH ; 2022
    In:  Archives of Industrial Hygiene and Toxicology Vol. 73, No. 1 ( 2022-04-07), p. 62-70
    Details
    In: Archives of Industrial Hygiene and Toxicology, Walter de Gruyter GmbH, Vol. 73, No. 1 ( 2022-04-07), p. 62-70
    Abstract: Patient immobilisation with volatile anaesthetics (VA) during radiotherapy is sometimes unavoidable. Although it is known that both VAs and ionising radiation can have nephrotoxic effects, there are no studies of their combined effects on DNA damage. The aim of this in vivo study was to address this gap by investigating whether 48 groups of healthy Swiss albino mice (totalling 240) would differ in kidney cell DNA damage response (alkaline comet assay) to isoflurane, sevoflurane, or halothane anaesthesia and exposure to 1 Gy or 2 Gy of ionising radiation. We took kidney cortex samples after 0, 2, 6, and 24 h of exposure and measured comet parameters: tail length and tail intensity. To quantify the efficiency of the cells to repair and re-join DNA strand breaks, we also calculated cellular DNA repair index. Exposure to either VA alone increased DNA damage, which was similar between sevoflurane and isoflurane, and the highest with halothane. In combined exposure (VA and irradiation with 1 Gy) DNA damage remained at similar levels for all time points or was even lower than damage caused by radiation alone. Halothane again demonstrated the highest damage. In combined exposure with irradiation of 2 Gy sevoflurane significantly elevated tail intensity over the first three time points, which decreased and was even lower on hour 24 than in samples exposed to the corresponding radiation dose alone. This study confirmed that volatile anaesthetics are capable of damaging DNA, while combined VA and 1 Gy or 2 Gy treatment did not have a synergistic damaging effect on DNA. Further studies on the mechanisms of action are needed to determine the extent of damage in kidney cells after longer periods of observation and how efficiently the cells can recover from exposure to single and multiple doses of volatile anaesthetics and radiotherapy.
    Type of Medium: Online Resource
    ISSN: 1848-6312
    URL: Article
    DOI: 10.2478/aiht-2022-73-3600
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2022
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  • 7
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    Online Resource
    Radioprotective Effects of Quercetin and Ethanolic Extract of Propolis in Gamma-Irradiated Mice
    Walter de Gruyter GmbH ; 2009
    In:  Archives of Industrial Hygiene and Toxicology Vol. 60, No. 2 ( 2009-06-1), p. 129-138
    Details
    In: Archives of Industrial Hygiene and Toxicology, Walter de Gruyter GmbH, Vol. 60, No. 2 ( 2009-06-1), p. 129-138
    Abstract: The aim of this study was to assess radioprotective effects of quercetin and the ethanolic extract of propolis (EEP) in CBA mice exposed to a single radiation dose 4 Gy ( 60 Co). The mice were treated with 100 mg kg -1 quercetin or EEP a day for three consecutive days either before (pre-treatment) or after gamma-irradiation (therapy). Leukocyte count was determined in blood drawn from the tail vein, and DNA damage in leukocytes was assessed using the alkaline comet assay. Genotoxic effects of the test compunds were also evaluated in non-irradiated mice. The levels of radioprotection provided by both test compounds were compared with those established in mice that were given chemical radioprotector S-(2-Aminoethy1)isothiouronium bromide hydrobromide (AET). Mice that received pre-treatment were less sensitive to irradiation. Mice given the post-irradiation therapy showed a slight but not significant increase in total leukocyte count over irradiated negative control. Quercetin showed better protective properties than EEP in both pre-treatment and therapy, and activated a higher number of leukocytes in non-irradiated mice. The alkaline comet assay suggests that both natural compounds, especially when given as pre-treatment, protect against primary leukocyte DNA damage in mice. At tested concentrations, EEP and quercetin were not genotoxic to non-irradiated mice. AET, however, caused a slight but not significant increase in DNA damage. Although the results of this study show the radioprotective potential of the test compounds, further investigation is needed to clarify the underlying protection mechanisms.
    Type of Medium: Online Resource
    ISSN: 0004-1254
    URL: Article
    DOI: 10.2478/10004-1254-60-2009-1908
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2009
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  • 8
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    Online Resource
    Cytotoxicity of Polyphenolic/Flavonoid Compounds in a Leukaemia Cell Culture
    Walter de Gruyter GmbH ; 2008
    In:  Archives of Industrial Hygiene and Toxicology Vol. 59, No. 4 ( 2008-12-1), p. 299-308
    Details
    In: Archives of Industrial Hygiene and Toxicology, Walter de Gruyter GmbH, Vol. 59, No. 4 ( 2008-12-1), p. 299-308
    Abstract: Flavonoidne sastavnice propolisa biološki su aktivne tvari koje posjeduju antioksidativna, protutumorska, imunomodulacijska i protuupalna svojstva. Istražili smo citotoksično djelovanje polifenolnih spojeva (kvercetina, kavene kiseline, krizina, naringenina i naringina) na različite linije leukemijskih stanica (MOLT, JURKAT, HL-60, RAJI, U937). Stanice su inkubirane u mediju RPMI-1640 obogaćenom 10%-tnim fetalnim telećim serumom, pri temperaturi od 37 °C u atmosferi s 5% CO 2 , uz dodatak polifenolnih/flavonoidnih spojeva različitih koncentracija (100 μg mL -1 , 50 μg mL -1 , 25 μg mL -1 i/ili 12,5 μg mL -1 ). Utvrđeno je da citotoksičnost flavonoida ovisi o vrsti i koncentraciji; najjači citotoksični učinak imaju kvercetin te krizin i kavena kiselina. Krizin i/ili naringenin primijenjeni na U937 i HL-60-stanice stimuliraju proliferaciju stanica, što upućuje na bifazni učinak istraživanih spojeva na monocitne leukemijske stanice. Dobiveni rezultati upućuju na potrebu daljnjih istraživanja učinkovitosti flavonoida na molekularnoj razini.
    Type of Medium: Online Resource
    ISSN: 0004-1254
    URL: Article
    DOI: 10.2478/10004-1254-59-2008-1903
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2008
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  • 9
    Online Resource
    Online Resource
    Brain Toxicokinetics of Prometryne in Mice
    Walter de Gruyter GmbH ; 2010
    In:  Archives of Industrial Hygiene and Toxicology Vol. 61, No. 1 ( 2010-03-1), p. 19-27
    Details
    In: Archives of Industrial Hygiene and Toxicology, Walter de Gruyter GmbH, Vol. 61, No. 1 ( 2010-03-1), p. 19-27
    Abstract: Prometryne is a methylthio- s -triazine herbicide. Significant trace amounts are found in the environment, mainly in water, soil, and food plants. The aim of this study was to establish brain and blood prometryne levels after single oral dose (1 g kg -1 ) in adult male and female mice. Prometryne was measured using the GC/MS assay at 1, 2, 4, 8, and 24 h after prometryne administration. Peak brain and blood prometryne values were observed 1 h after administration and they decreased in a time-dependent manner. Male mice had consistently higher brain and blood prometryne levels than female mice. The observed prometryne kinetics was similar to that reported for the structurally related herbicide atrazine.
    Type of Medium: Online Resource
    ISSN: 0004-1254
    URL: Article
    DOI: 10.2478/10004-1254-61-2010-1986
    Language: Unknown
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2010
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  • 10
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    Online Resource
    Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study
    Walter de Gruyter GmbH ; 2016
    In:  Acta Pharmaceutica Vol. 66, No. 3 ( 2016-9-1), p. 411-421
    Details
    In: Acta Pharmaceutica, Walter de Gruyter GmbH, Vol. 66, No. 3 ( 2016-9-1), p. 411-421
    Abstract: Olive leaf extract is characterized by a high content of polyphenols (oleuropein, hydroxytyrosol and their derivatives), which is associated with its therapeutic properties. The objective of the present research was to evaluate the antifungal activity of olive leaf extract against Candida albicans ATCC 10231 and C. dubliniensis CBS 7987 strains. Minimum inhibitory concentrations (MIC) of the extract were determined by several in vitro assays. The extract showed a concentration depended effect on the viability of C. albicans with MIC value of 46.875 mg mL -1 and C. dubliniensis with MIC value 62.5 mg mL -1 . Most sensitive methods for testing the antifungal effect of the extracts were the trypan blue exclusion method and fluorescent dye exclusion method while MIC could not be determined by the method according to the EUCAST recommendation suggesting that herbal preparations contain compounds that may interfere with this susceptibility testing. The fluorescent dye exclusion method was also used for the assessment of morphological changes in the nuclei of treated cells. According to the obtained results, olive leaf extract is less effective against the tested strains than hydroxytyrosol, an olive plant constituent tested in our previous study.
    Type of Medium: Online Resource
    ISSN: 1846-9558
    URL: Article
    DOI: 10.1515/acph-2016-0033
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2016
    detail.hit.zdb_id: 2255569-9
    SSG: 15,3
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