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  • Walter de Gruyter GmbH  (2)
  • Biology  (2)
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  • Walter de Gruyter GmbH  (2)
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  • Biology  (2)
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  • 1
    Online Resource
    Online Resource
    Porin of Rhodobacter capsulatus: Biochemical and Functional Characterization
    Walter de Gruyter GmbH ; 1990
    In:  Zeitschrift für Naturforschung C Vol. 45, No. 6 ( 1990-6-1), p. 576-582
    Details
    In: Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 45, No. 6 ( 1990-6-1), p. 576-582
    Abstract: The major outer membrane protein of Rhodobacter capsulatus 37 b4 (capsule-free) was iso­lated. Strong porin-activity was observed after reconstitution into artificial lipid bilayer mem­branes with a single channel conductance of 3.15 nS in Im KC1. The porin migrated as a broad, single band (M r above 90,000) on sodium dodecyl sulfate polyacrylamide gel electro­ phoresis and dissociated into a single species of polypeptides (M r 36,000) on treatment with EDTA (10 mM at 30 °C, 20 min) or by heating (100 °C, 5 min). Analytical ultracentrifugation studies demonstrated the native porin to be a trimer. The monomers chromatofocused as a single, sharp peak on fast performance liquid chromatography and only one band, corre­ sponding to an isoelectric point of about 4.0, was obtained on isoelectric focusing. Gas-phase sequencing of the 23 N-terminal residues revealed Glu-Val-Lys-Leu-Ser-Gly-Asp-Ala-Arg-Met-Gly-Val-Met-Tyr-Asn-Gly-Asp-Asp-X-Asn-Phe-Ser-Ser.
    Type of Medium: Online Resource
    ISSN: 1865-7125 , 0939-5075
    URL: Article
    DOI: 10.1515/znc-1990-0602
    RVK:
    WA 15000
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 1990
    detail.hit.zdb_id: 2078107-6
    SSG: 12
    Location Call Number Limitation Availability
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    Online Resource
  • 2
    Online Resource
    Online Resource
    Pore-Forming Activity of Outer Membrane Extracts from the Unicellular Cyanobacterium Synechocystis sp. PCC 6714
    Walter de Gruyter GmbH ; 1989
    In:  Zeitschrift für Naturforschung C Vol. 44, No. 1-2 ( 1989-2-1), p. 165-169
    Details
    In: Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 44, No. 1-2 ( 1989-2-1), p. 165-169
    Abstract: Cell walls of the unicellular cyanobacterium Synechocystis sp. PCC 6714, isolated from cell homogenates, were found to be unusually resistant against extraction with various detergents, organic solvents, chaotropic agents, and proteases. The major outer membrane proteins (M r 67,000; 61,000; 94,000) were solubilized by differential SDS-extraction and purified by preparative SDS-PAGE. The extracted proteins, reconstituted into lipid bilayer membranes, formed two types of pores with single-channel conductances of 2.2 nS (pore diameter of 1.4 nm) and 0.3 nS (pore diameter not determined), respectively. Carotenoids and lipopolysaccharide were found to be associated with the extracted major proteins.
    Type of Medium: Online Resource
    ISSN: 1865-7125 , 0939-5075
    URL: Article
    DOI: 10.1515/znc-1989-1-225
    RVK:
    WA 15000
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 1989
    detail.hit.zdb_id: 2078107-6
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
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