In:
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, The Royal Society, Vol. 374, No. 2079 ( 2016-10-28), p. 20150371-
Abstract:
Quantitative analysis with mass spectrometry (MS) is important but challenging. Matrix-assisted laser desorption/ionization (MALDI) coupled with time-of-flight (TOF) MS offers superior sensitivity, resolution and speed, but such techniques have numerous disadvantages that hinder quantitative analyses. This review summarizes essential obstacles to analyte quantification with MALDI-TOF MS, including the complex ionization mechanism of MALDI, sensitive characteristics of the applied electric fields and the mass-dependent detection efficiency of ion detectors. General quantitative ionization and desorption interpretations of ion production are described. Important instrument parameters and available methods of MALDI-TOF MS used for quantitative analysis are also reviewed. This article is part of the themed issue ‘Quantitative mass spectrometry’.
Type of Medium:
Online Resource
ISSN:
1364-503X
,
1471-2962
DOI:
10.1098/rsta.2015.0371
Language:
English
Publisher:
The Royal Society
Publication Date:
2016
detail.hit.zdb_id:
208381-4
detail.hit.zdb_id:
1462626-3
SSG:
11
SSG:
5,1
SSG:
5,21
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