In:
ECS Meeting Abstracts, The Electrochemical Society, Vol. MA2016-02, No. 52 ( 2016-09-01), p. 3885-3885
Abstract:
Food safety is a global issue directly related with human life and foodborne diseases take a major crisis on health. In this reason, a reliable and cost-effective device is required as a solution to the prevention and recognition of problems concerned with health and safety. Conventional and standard bacterial detection methods such as culture and colony counting methods, immunology-based methods and polymerase chain reaction based methods, may take up to several hours or even a few days to yield an answer. Obviously this is inadequate, and recently many researchers are focusing towards the progress of rapid methods. Here, a fully integrated electrochemical microfluidic chip was developed for identification of food-poisoning bacteria, consisting of three compartments such as film-based gene amplification, three-dimensional micro mixer for homogeneous mixing, the detection electrodes for obtaining electrochemical signal. Just after loading genomic DNA into a developed microfluidic chip, which is extracted from cells such as Esterichia coli. O157:H7, Salmonella enteritidis, Staphylococcus aureus, Clostridium perfringens and Listeria monocytogenes, gene is amplified during PCR procedure in film-based reaction chamber followed by mixing amplified PCR product with electroactive reagent. And then electrochemical signal was produced on electrode surface. If there is no corresponding target pathogen, no electrochemical signal was produced. Furthermore, we can detect one or multiple pathogens at the same time by reason that 5 kinds of foodborne pathogens are amplified in parallel and identify the target pathogens simultaneously. With just one-step including sample injection, identification of different varieties of food-borne pathogens was successfully performed in a microfluidic chip within an hour. Owing to its simplicity and rapidity, the developed microfluidic device holds great promise for identification of several species in facility-limited environment or POCT applications.
Type of Medium:
Online Resource
ISSN:
2151-2043
DOI:
10.1149/MA2016-02/52/3885
Language:
Unknown
Publisher:
The Electrochemical Society
Publication Date:
2016
detail.hit.zdb_id:
2438749-6
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