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  • The American Society for Biochemistry and Molecular Biology (ASBMB)  (1)
  • Wiley-Blackwell  (1)
  • 1
    Publication Date: 2016-06-29
    Description: A two-dimensional array of fourteen seafloor pressure sensors was deployed to measure properties of tidally-generated, nonlinear, high-frequency internal waves over a 14-km by 12-km area west of Stellwagen Bank in Massachusetts Bay during summer 2009. Thirteen high-frequency internal wave packets propagated through the region over 6.5 days (one packet every semidiurnal cycle). Propagation speed and direction of wave packets were determined by triangulation, using arrival times and distances between triads of sensor locations. Wavefront curvature ranged from straight to radially spreading, with wave speeds generally faster to the south. Waves propagated to the southwest, rotating to more westward with shoreward propagation. Linear theory predicts a relationship between kinetic energy and bottom pressure variance of internal waves that is sensitive to sheared background currents, water depth, and stratification. By comparison to seafloor acoustic Doppler current profiler measurements, observations nonetheless show a strong relationship between kinetic energy and bottom pressure variance. This is presumably due to phase-locking of the wave packets to the internal tide that dominates background currents and to horizontally uniform and relatively constant stratification throughout the study. This relationship was used to qualitatively describe variations in kinetic energy of the high-frequency wave packets. In general, high-frequency internal wave kinetic energy was greater near the southern extent of wavefronts and greatly decreased upon propagating shoreward of the 40-m isobath. This article is protected by copyright. All rights reserved.
    Print ISSN: 0148-0227
    Topics: Geosciences , Physics
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  • 2
    Publication Date: 2014-12-06
    Description: Extracellular heparanase activity releases growth factors and angiogenic factors from heparan sulfate (HS) storage sites and alters the integrity of the extracellular matrix. These activities lead to a loss of normal cell matrix adherent junctions and correlate with invasive cellular phenotypes. Elevated expression of heparanase is associated with several human cancers and with vascular remodeling. Heparanase cleaves only a limited fraction of glucuronidic linkages in HS. There have been few investigations of the functional consequences of heparanase activity, largely due to the heterogeneity and complexity of HS. Here, we report a liquid chromatography-mass spectrometry (LC-MS)-based approach to profile the terminal structures created by heparanase digestion and reconstruct the heparanase cleavage sites from the products. Using this method, we demonstrate that heparanase cleaves at the non-reducing side of highly sulfated HS domains, exposing cryptic growth factor binding sites. This cleavage pattern is observed in HS from several tissue sources, regardless of overall sulfation degree, indicating a common recognition pattern. We further demonstrate that heparanase cleavage of HS chains leads to increased ability to support FGF2-dependent cell proliferation. These results suggest a new mechanism to explain how heparanase might potentiate the uncontrolled cell proliferation associated with cancer through its ability to activate nascent growth factor-promoting domains within HS.
    Print ISSN: 0021-9258
    Electronic ISSN: 1083-351X
    Topics: Biology , Chemistry and Pharmacology
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