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  • Articles  (17)
  • Wiley-Blackwell  (17)
  • The American Society for Biochemistry and Molecular Biology (ASBMB)
  • The American Society for Pharmacology and Experimental Therapeutics (ASPET)
  • Physics  (16)
  • Architecture, Civil Engineering, Surveying  (1)
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  • Articles  (17)
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  • 1
    Electronic Resource
    Electronic Resource
    Preparation of hydrolyzed pH responsive ion track membraneSystematic IUPAC name: diallyl oxydiethylene dicarbonate. (1995)
    Weinheim : Wiley-Blackwell
    Macromolecular Rapid Communications 16 (1995), S. 47-51 
    Details
    ISSN: 1022-1336
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The porous copolymer films of N-methacryloyl-L-alanine methyl ester and diethyleneglycol-bis-allylcarbonate (diallyl oxydiethylene dicarbonate) were hydrolyzed with 1 M NaOH solution for 40 min at 25°C. The pores of the films showed pH response in diameter. The pore diameter of 3,7 μm in the buffer solution of pH 3 was closed completely at pH 5.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/marc.1995.030160109
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Influence of materials and structure on performance of a football helmet (1987)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 27 (1987), S. 1113-1120 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A nonlinear finite element model for the static analysis of football helmets is developed as a first step in a methodology for the mathematical modeling of helmets. Polycarbonate and acrylonitrile-butadiene-styrene are considered as the shell material for the helmet. Such a methodology can be used to predict helmet response to loading and impact and can be incorporated in a computer program studying injuries caused by helmeted head collisions in football. Numerical results in terms of force-deflection curves, maximum stresses, and work of deformation are given. The influence of the polymeric material forming the helmet shell on energy absorption is studied. The effect of the internal padding material and suspension system is also considered.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760271502
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A method for counting disulfide bridges in small proteins by reduction with mercaptoethanol and electrospray mass spectrometry (1995)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 30 (1995), S. 1562-1566 
    Details
    ISSN: 1076-5174
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: A simple and rapid method for counting the number of internal disulfide bridges in a protein by incubation with 2-mercaptoethanol and electrospray mass spectrometry analysis of the products was developed. 2-Mercaptoethanol yields intermediate mixed disulfides during reduction of a protein. This results in a molecular weight increase of the protein by 78 Da per disulfide bond, which can easily be determined by electrospray mass spectrometry (ESMS). The number of mercaptoethanol adducts observed by ESMS reveals the number of disulfide bridges in the peptide or protein. Since the protein-mercaptoethanol-disulfide bonds are themselves further reduced by excess mercaptoethanol, the course of the reaction has to be followed in order to detect the maximum number of intermediates. Owing to the volatility of mercaptoethanol, samples can be taken out of the reaction solution for MS analysis without prior purification. Successful experiments were carried out using proteins with one, two, four or six S—S-bonds, covering a mass range from about 1 to over 23 kDa.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jms.1190301107
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Elektronenmikroskopische und mikroanalytische Untersuchungen zur Veränderung der übermolekularen Struktur von mit Natriumhydroxid-Lösungen und Schwefelsäure behandelter Cellulose (1982)
    Weinheim : Wiley-Blackwell
    Acta Polymerica 33 (1982), S. 43-48 
    Details
    ISSN: 0323-7648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: The changes and the conditions of destruction of the supramolecular structure of cellulose caused by the action of NaOH and sulfuric acid of different concentration have been investigated by electron microscopy. With different kinds of cellulose the destruction of the microfibrillar structure was observed to occur within various ranges of concentration. The crystalline structures formed by the treatment with the reagents were investigated by electron diffraction and their elemental composition was determined by x-ray microanalysis.
    Notes: Durch elektronenmikroskopische Untersuchungen werden die Veränderung der übermolekularen Struktur der Cellulose bei der Einwirkung von NaOH und Schwefelsäure unterschieflicher Konzentration verfolgt und die Bedingungen ihrer Zerstörung ermittelt. Die Zerstörung der mikrofibrillaren Struktur durch NaOH-Lösungen bzw. Schwefelsäure liegt bei den einzelnen Cellulosetypen in verschiedenen Konzentrationsbereichen. Die durch die Behandlung mit den Reagenzien gebildeten kristallinen Gebilde werden mittels Röntgenmikroanalyse auf ihre Elementzusammensetzung und mittels Elektronenbeugung auf ihre kristalline Struktur untersucht.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/actp.1982.010330109
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  • 5
    Electronic Resource
    Electronic Resource
    Physical characterization of cellulose xanthate in solution (1951)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Polymer Science 7 (1951), S. 261-276 
    Details
    ISSN: 0022-3832
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The intrinsic viscosity of cellulose xanthate solutions was studied as a function of the electrolyte content of the solvent, and found to decrease sharply with increase in electrolyte concentration. All electrolytes tested except sodium and lithium hydroxides produced essentially the same reduction in intrinsic viscosity for solutions of equivalent ionic strenghts, indicating that the effect was largely one of interaction between the solvent ions and the charged xanthate groups. Hydroxides induced a greater viscosity reduction. The reason for this was not fully resolved; however, indications were obtained that the hydroxides, in addition to behaving as electrolytes toward the charged xanthate groups, also interact with the hydroxyl groups on the parent cellulose chain.Light scattering measurements, as well as data on the variation of the intrinsic viscosity with molecular weight in various solvents, showed that the change in intrinsic viscosity with electrolyte concentration could be accounted for by a change in configuration, or degree of coiling of the molecule. Calculations indicated that in pure water cellulose xanthate should approach a rigid rod configuration whereas in 6% caustic coiling occurs and the molecule resembles more a random configuration.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pol.1951.120070212
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  • 6
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    LA-ICP-MS depth profiling perspective on cleaning protocols for elemental analyses in planktic foraminifers (2013)
    Wiley-Blackwell
    Details
    Publication Date: 2013-05-09
    Description: Measurements of trace metal ratios in foraminiferal calcite are routinely used to reconstruct paleoceanographic conditions. Analyses using solution-based inductively coupled plasma mass spectrometry (ICP-MS) require dissolution of the entire foraminifer shell. The potential exists for contamination from adherent clays, mineralized coatings, and other diagenetic components that confound the biogenic trace metal signal. We present results from a cleaning experiment on fossil specimens of the planktic foraminifer Orbulina universa that were cracked into several shell fragments and subjected to different cleaning protocols. We use LA-ICP-MS depth profiling to evaluate the effects of reductive, oxidative, and chelating (DTPA) cleaning protocols on shell Mg/Ca and Ba/Ca ratios. Using the natural pattern of intrashell Mg/Ca heterogeneity exhibited by O. universa , we demonstrate that reductive and oxidative cleaning can dissolve shell calcite from available surfaces, although intrashell Mg/Ca minima and maxima are unaffected. High-resolution depth profiles can be used to identify areas of heterogeneous intrashell Ba/Ca, which can be excluded from computations of whole-shell Ba/Ca. The size and density of shell pores plays a major role in the degree of contamination from sedimentary material. We demonstrate an approach for computing whole-shell Me/Ca ratios from LA-ICP-MS depth profiles that accounts for potential contamination and diagenetic overprinting.
    Electronic ISSN: 1525-2027
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Published by Wiley-Blackwell on behalf of American Geophysical Union (AGU).
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  • 7
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    Identification of frequent La Niña events during the early 1800s in the east equatorial Pacific (2015)
    Wiley-Blackwell
    Details
    Publication Date: 2015-02-17
    Description: We report measurements of near-monthly Δ 14 C and δ 18 O during selected decades from an east equatorial Pacific coral that grew during the past four centuries. We find that El Niño events occurred regularly during the late 1700s. During the early 1800s, El Niño events occurred less often, and La Niña conditions prevailed, which were accompanied by unprecedented, low cool season Δ 14 C values and high cool season δ 18 O values. These results indicate that shallow overturning water (e.g. Central Mode Water) from the North Pacific was likely an important source of water to the Galapagos area during the early 1800s.
    Print ISSN: 0094-8276
    Electronic ISSN: 1944-8007
    Topics: Geosciences , Physics
    Published by Wiley-Blackwell on behalf of American Geophysical Union (AGU).
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  • 8
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    Optical laser systems at the Linac Coherent Light Source (2015)
    Wiley-Blackwell
    Details
    Publication Date: 2015-04-22
    Description: Ultrafast optical lasers play an essential role in exploiting the unique capabilities of recently commissioned X-ray free-electron laser facilities such as the Linac Coherent Light Source (LCLS). Pump–probe experimental techniques reveal ultrafast dynamics in atomic and molecular processes and reveal new insights in chemistry, biology, material science and high-energy-density physics. This manuscript describes the laser systems and experimental methods that enable cutting-edge optical laser/X-ray pump–probe experiments to be performed at LCLS.
    Print ISSN: 0909-0495
    Electronic ISSN: 1600-5775
    Topics: Geosciences , Physics
    Published by Wiley-Blackwell on behalf of International Union of Crystallography (IUCr).
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  • 9
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    Expression, purification, crystallization and preliminary crystallographic analysis of a GH20 β-N-acetylglucosaminidase from the marine bacterium Vibrio harveyi (2015)
    Wiley-Blackwell
    Details
    Publication Date: 2015-04-08
    Description: Vibrio harveyi β- N -acetylglucosaminidase ( Vh GlcNAcase) is a new member of the GH20 glycoside hydrolase family responsible for the complete degradation of chitin fragments, with N -acetylglucosamine (GlcNAc) monomers as the final products. In this study, the crystallization and preliminary crystallographic data of wild-type Vh GlcNAcase and its catalytically inactive mutant D437A in the absence and the presence of substrate are reported. Crystals of wild-type Vh GlcNAcase were grown in 0.1  M sodium acetate pH 4.6, 1.4  M sodium malonate, while crystals of the D437A mutant were obtained in 0.1  M bis-tris pH 7.5, 0.1  M sodium acetate, 20% PEG 3350. X-ray data from the wild-type and the mutant crystals were collected at a synchrotron-radiation light source and were complete to a resolution of 2.5 Å. All crystals were composed of the same type of dimer, with the substrate N , N ′-diacetylglucosamine (GlcNAc 2 or diNAG) used for soaking was cleaved by the active enzyme, leaving only a single GlcNAc molecule bound to the protein.
    Electronic ISSN: 1744-3091
    Topics: Biology , Chemistry and Pharmacology , Geosciences , Physics
    Published by Wiley-Blackwell on behalf of International Union of Crystallography (IUCr).
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  • 10
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    Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod–Zwilch–ZW10 (RZZ) complex (2015)
    Wiley-Blackwell
    Details
    Publication Date: 2015-04-08
    Description: The spindle-assembly checkpoint (SAC) monitors kinetochore–microtubule attachment during mitosis. In metazoans, the three-subunit Rod–Zwilch–ZW10 (RZZ) complex is a crucial SAC component that interacts with additional SAC-activating and SAC-silencing components, including the Mad1–Mad2 complex and cytoplasmic dynein. The RZZ complex contains two copies of each subunit and has a predicted molecular mass of ∼800 kDa. Given the low abundance of the RZZ complex in natural sources, its recombinant reconstitution was attempted by co-expression of its subunits in insect cells. The RZZ complex was purified to homogeneity and subjected to systematic crystallization attempts. Initial crystals containing the entire RZZ complex were obtained using the sitting-drop method and were subjected to optimization to improve the diffraction resolution limit. The crystals belonged to space group P 3 1 (No. 144) or P 3 2 (No. 145), with unit-cell parameters a = b = 215.45, c = 458.7 Å, α = β = 90.0, γ = 120.0°.
    Electronic ISSN: 1744-3091
    Topics: Biology , Chemistry and Pharmacology , Geosciences , Physics
    Published by Wiley-Blackwell on behalf of International Union of Crystallography (IUCr).
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