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  • The American Association for Cancer Research (AACR)  (18)
  • Wiley-Blackwell  (18)
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  • 1
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cells of the follicular epithelium of the ovarioles of the mosquito, Aedes aegypti (L.), and of the stable fly, Stomoxys calcitrans (L.), have been found to be interconnected by cytoplasmic bridges. Cytoplasmic bridges have been reported between sibling gamete cells of both male and female insects, but these bridges have not been previously reported to exist between somatic cells of insects.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 2 (1979), S. 613-616 
    ISSN: 0935-6304
    Keywords: Gas chromatography, capillary, glass ; 5 mikrometer quartz powder roughening layer fused onto boro silicate glass during drawing process ; Complete procedure given reproducible preparation of stable thick film capillaries ; Static impregnation with 1.5% (w/v) rubber solutions possible ; Very low column bleed, ideal for GC-MS ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A detailed method for the routine preparation of glass capillary columns is presented. The method consists of coating a glass tube with quartz powder prior to pulling the tube into a capillary. The inner surface of the capillary consists of an even distribution of quartz particles fused to the walls. This surface has been found readily deactivated by standard procedures and ideal for the preparation of thick-film glass capillary columns. The method has been thoroughly tested in two independent laboratories to ensure that the procedures described are reproducible.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 5 (1982), S. 432-434 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Glass capillary columns ; Radiation induced polymerization ; Crosslinking ; Immobilized phases ; Column activity ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 3 (1980), S. 91-91 
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Static coating technique details ; End-sealing without failure, under ethanol ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 18 (1972), S. 457-458 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 31 (1992), S. 253-257 
    ISSN: 1040-452X
    Keywords: Glucose ; Pyruvate ; Lactate ; Embryo culture ; Sheep ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Glucose utilization by sheep embryos was examined in 8-cell (N = 36) and blastocyst (N = 36) stages, by measuring conversion of [5-3H]glucose to 3H2O. Fifty percent glucose utilization occurred at 0.79 ± 0.69 mM for 8-cell embryos and ∼0.06 ± 0.15 mM for blastocysts. Development of 1- and 2-cell sheep embryos (N = 264) was examined under different glucose concentrations (0, 1.5, 3, or 6 mM) and in the presence or absence of 0.33 mM pyruvate and 3.3 mM lactate (PL). Overall, the presence of glucose was detrimental (P 〈 0.001) to embryonic development. By contrast, the presence of pyruvate and lactate was beneficial (P〈0.001) to development. An interaction was observed between the concentration of glucose and presence or absence of PL (P 〈0.05). An optimum level of glucose occurs at 0-3 mM in the presence of PL (P ±0.1). Development to the blastocyst stage was observed in medium when supplemented with amino acids and albumin alone. Thus, glucose metabolism is not critical for embryonic development, but beneficial at low concentrations. High concentrations can inhibit development, possibly by inhibiting the tricarboxylic acid (TCA) cycle. Sheep embryos may also be using amino acids as an energy source for development.
    Additional Material: 1 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Applied Organometallic Chemistry 12 (1998), S. 643-650 
    ISSN: 0268-2605
    Keywords: tributyltin ; sediment ; degradation ; coastal British Columbia ; Chemistry ; Industrial Chemistry and Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Butyltin concentrations in the sediments of two coastal areas of British Columbia, Canada, are reported. Two recent box cores from the deepest basin in the Strait of Georgia were sectioned and analyzed by GC-atomic emission spectrometry. No butyltin compounds were detected above 0.5 μgSn kg-1 (dry weight) in either core. These results are compared to those for a previous (1991) core from the same area. In that study, tributyltin (TBT) concentrations were in the range 1-2 μgSn kg-1 down the core and were higher than those of either of the degradation products, dibutyltin (DBT) and monobutyltin (MBT). Radioisotope dating (210Po-210Pb counting methods) was used to establish the rates of sedimentation of 0.25 and 2.6 cm y-1 at the two sites. Data suggest that a combination of rapid deposition of new, less-contaminated material and degradation of previously deposited butyltin compounds has resulted in the observed absence. Thirty-three surface sediments from the northern BC coastal harbor at Prince Rupert, collected in 1995, were analyzed for butyltin residues by GC-FPD. Concentrations of TBT, DBT and MBT were in the ranges from below the appropriate limit of detection (LOD) to 1262, to 109 and to 37 μgSn kg-1, respectively. TBT/DBT ratios ranged from 0.2 to 62 with most above unity, indicating that there is continuing fresh input of TBT. The sources are almost exclusively large ocean-going vessels that use the harbor for long-term anchorage. These findings are discussed with reference to the global TBT status. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 3 Ill.
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 163 (1995), S. 631-635 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Nonsedimentable lipid-protein particles have been isolated from intestinal tissue of the American cockroach, Periplaneta americana. Most of the particles were within the range 30-50 nm in diameter and appear to originate from larger structures. Lipid analysis of the particles showed them to be enriched in neutral lipid components relative to microsomal membranes. Specifically, there is a decline in the amounts of phosphatidylcholine and phosphatidylethanolamine in the nonsedimentable particles compared with the microsomal membranes. Also, in contrast to microsomal membranes, the particles have a higher content of phosphatidic acid along with 1,2- and 1,3-diacyglycerols, free fatty acids and an unidentified lipid that co-migrates with sterol ester, wax ester and hydrocarbon standards in thin layer chromatograms. The cytosol, separated from the particles by ultrafiltration, contained phosphatidic acid, free fatty acids and the unidentified lipid. By contrast, the composition of neutral lipids in the cytosol resembles that of the particles. SDS - PAGE analysis of microsomal membranes, the particles and particle free cytosol shows an enrichment of low molecular weight proteins in the particles and cytosol. The particles and cytosol appear to possess proteolytic activity that is distinguishable from that of corresponding microsomal membranes since the incubation of these components with BSA resulted in the formation of distinct polypeptides. Many characteristics of these particles resemble those of the deteriosomes that have been isolated from plant tissue. © 1995 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 14 (1998), S. 565-571 
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; electroporation ; transformation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-fold improvement in transformation frequency was achieved with several mutant strains of S. cerevisiae that transformed poorly by conventional procedures. Both DTT and lithium acetate were necessary for maximal transformation frequencies. Pretreatment with lithium and DTT also resulted in an ∼3·5-fold increase in the electroporation transformation frequency of the pathogenic fungus Candida albicans. © 1998 John Wiley & Sons, Ltd.
    Additional Material: 1 Ill.
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