In:
Virology Journal, Springer Science and Business Media LLC, Vol. 3, No. 1 ( 2006-12)
Abstract:
It was reported that elevation of the intracellular concentration of free Ca 2+ ([Ca 2+ ]i) by a calcium ionophore increased the release of herpes simplex virus type 1 (HSV-1). Freely diffusible hydrogen peroxide (H 2 O 2 ) is implied to alter Ca 2+ homeostasis, which further enhances abnormal cellular activity, causing changes in signal transduction, and cellular dysfunction. Whether H 2 O 2 could affect [Ca 2+ ]i in HSV-1-infected cells had not been investigated. Results H 2 O 2 treatment increased the amount of cell-free virus and decreased the proportion of viable cells. After the treatment, an elevation in [Ca 2+ ]i was observed and the increase in [Ca 2+ ]i was suppressed when intracellular and cytosolic Ca 2+ were buffered by Ca 2+ chelators. In the presence of Ca 2+ chelators, H 2 O 2 -mediated increases of cell-free virus and cell death were also diminished. Electron microscopic analysis revealed enlarged cell junctions and a focal disintegration of the plasma membrane in H 2 O 2 -treated cells. Conclusion These results indicate that H 2 O 2 can elevate [Ca 2+ ]i and induces non-apoptotic cell death with membrane lesions, which is responsible for the increased release of HSV-1 from epithelial cells.
Type of Medium:
Online Resource
ISSN:
1743-422X
DOI:
10.1186/1743-422X-3-62
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2006
detail.hit.zdb_id:
2160640-7
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