In:
Photochemical & Photobiological Sciences, Springer Science and Business Media LLC, Vol. 14, No. 5 ( 2015-05), p. 1005-1012
Abstract:
Sub-diffraction imaging of plasma membrane localized proteins, such as the SNARE ( S oluble N SF A ttachment Protein R eceptor) proteins involved in exocytosis, in fixed cells have resulted in images with high spatial resolution, at the expense of dynamical information. Here, we have imaged localized fluorescence bursts of DRONPA-fused SNAP-25 molecules in live chromaffin cells by Total Internal Reflection Fluorescence (TIRF) imaging. We find that this method allows tracking protein cluster dynamics over relatively long times (∼20 min.), partly due to the diffusion into the TIRF field of fresh molecules, making possible the simultaneous identification of cluster size, location and temporal evolution. The results indicate that the DRONPA-fused SNAP-25 clusters display rich dynamics, going from staying constant to disappearing and reappearing in specific cluster domains within minutes.
Type of Medium:
Online Resource
ISSN:
1474-905X
,
1474-9092
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2015
detail.hit.zdb_id:
2072584-X
SSG:
12
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