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  • 1
    Electronic Resource
    Electronic Resource
    Micropropagation ofPanax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets (1997)
    Springer
    Plant cell reports 16 (1997), S. 450-453 
    Details
    ISSN: 1432-203X
    Keywords: Micropropagation ; Panax notoginseng ; RAPD analysis ; Genetic homogeneity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitable medium for optimal root formation proved to be MS medium supplemented with 1-naphthaleneacetic acid (NAA). Total DNA was extracted from the leaves of the regenerated plantlets ofP. notoginseng. Analysis of random-amplified polymorphic DNA (RAPD) using 21 arbitrary oligonucleotide 10-mers, showed the genetic homogeneity ofP. notoginseng. The amplification products were monomorphic for all of the plantlets ofP. notoginseng regenerated by embryogenesis, suggesting that somatic embryogenesis can be used for clonal micropropagation of this plant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01092764
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  • 2
    Electronic Resource
    Electronic Resource
    Distribution of free and conjugated ecdysteroids between follicle cell sheath and ooplasm in oocytes of the cockroachNauphoeta cinerea (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 496-498 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary It is shown that both follicle cell sheath and ooplasm contain only small quantities ofHelix-hydrolyzable ecdysteroid conjugates and that 20-hydroxy-ecdysone is the predominant free ecdysteroid. Its concentration is very high in the follicle cell sheath immediately before chorion formation (6825 ng/g) and much lower after chorion formation (150 ng/g), while it is 234 ng/g and 95 ng/g in the ooplasm at the same stages respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01952404
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  • 3
    Electronic Resource
    Electronic Resource
    Retina accumulates more glucose than does the embryologically similar cerebral cortex in diabetic rats (2000)
    Springer
    Diabetologia 43 (2000), S. 1417-1423 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Diabetic retinopathy, hyperglycaemia, Amadori product, glucose transporter, brain, retina.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aims/hypothesis. The retina is embryologically similar to cerebral cortex and the tissues of both are exposed to similar blood glucose concentrations. Nevertheless, in diabetes the retina develops metabolic abnormalities and microvascular lesions from which cerebrum seems relatively protected. We directly compared glucose concentrations and expression of GLUT-1 (the major carrier transporting glucose from blood into the neural retina and cerebrum) in the two tissues from normal and diabetic rats.¶Methods. Tissue and intracellular glucose were measured using two methods: direct assay of glucose and assay of Amadori products on intracellular proteins. The expression of GLUT-1 was measured using western blots in tissue and in the isolated endothelial luminal membrane of the two vascular beds.¶Results. Both methods assessing intracellular glucose indicate that intracellular concentrations of glucose in diabetes increased significantly in the retina but not in cerebral cortex. Concentrations of free glucose and Amadori product in retinas of diabetic animals were increased above normal by 334 % and 122 %, respectively, whereas there was no statistically significant increase in either parameter in the cerebral cortex of diabetic animals. In contrast to the observed increase in glucose in the retina in diabetes, expression of GLUT-1 on the luminal plasmalemma of the retinal vascular endothelium and in homogenates of whole retina decreased to a statistically significant extent (55 % and 36 %, respectively compared to normal). In the luminal cell membrane of the cerebral vasculature, diabetes did not decrease expression of GLUT-1 but tended to increase it slightly.¶Conclusions/interpretation. Even among tissues that do not require insulin for glucose uptake, tissue glucose concentration varies in diabetes. The greater increase in glucose concentration in retina than in cerebrum in diabetes probably contributes to the tissue differences in biochemical and histopathologic sequelae of the disease. The expression of GLUT-1 in the microvasculature is unlikely to account for the differences in tissue glucose between retina and cerebrum. [Diabetologia (2000) 43: 1417–1423]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250051548
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  • 4
    Electronic Resource
    Electronic Resource
    Raman spectroscopy of strained GeSi alloys deposited on Ge substrates (1996)
    Springer
    Applied physics 62 (1996), S. 387-390 
    Details
    ISSN: 1432-0630
    Keywords: 78.30.-j ; 61.55.Hg ; 81.15.Gh
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The Raman scattering method has been successfully used to investigate the properties of GeSi alloys deposited on Ge substrates in this paper. The effect of Si composition and strain in the GeSi alloy on the Raman shifts of Ge-Ge, Ge-Si and Si-Si phonon modes is studied. The relationship between them have been derived by the assumption that the Raman shifts is nearly linear with Si composition and strain in the GeSi alloys. The experimental data show reasonable agreement with the fits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01594238
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  • 5
    Electronic Resource
    Electronic Resource
    An optical differential reflectance study of adsorption and desorption of xenon and deuterium on Ni(111) (1996)
    Springer
    Applied physics 63 (1996), S. 1-8 
    Details
    ISSN: 1432-0630
    Keywords: PACS: 78.66; 82.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract.  We studied adsorption and desorption of Xe and deuterium on Ni(111) using an optical differential reflectance technique. The main findings are: (i) the differential reflectance varies almost linearly with the surface densities of deuterium and Xe adatoms, and the signals can be described well with a three-layer model and the known dielectric responses of the surface layers: (ii) the adsorption of deuterium at T=120 K follows the Langmuir kinetics, while the adsorption of Xe at T=38 K follows the zeroth-order kinetics; (iii) near T=70 K, the rate of Xe desorption is almost coverage-independent with an activation energy of E des=4.4±0.2 kcal/mol. Our analysis suggests that the Xe desorption is likely to be dominated by the escape rate from the corners of two-dimensional Xe islands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003390050345
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  • 6
    Electronic Resource
    Electronic Resource
    An optical differential reflectance study of adsorption and desorption of xenon and deuterium on Ni(111) (1996)
    Springer
    Applied physics 63 (1996), S. 1-8 
    Details
    ISSN: 1432-0630
    Keywords: 78.66 ; 82.65
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract We studied adsorption and desorption of Xe and deuterium on Ni(111) using an optical differential reflectance technique. The main findings are: (i) the differential reflectance varies almost linearly with the surface densities of deuterium and Xe adatoms, and the signals can be described well with a three-layer model and the known dielectric responses of the surface layers: (ii) the adsorption of deuterium atT = 120 K follows the Langmuir kinetics, while the adsorption of Xe atT = 38 K follows the zeroth-order kinetics; (iii) nearT = 70 K, the rate of Xe desorption is almost coverage-independent with an activation energy ofE des = 4.4 ± 0.2 kcal/mol. Our analysis suggests that the Xe desorption is likely to be dominated by the escape rate from the corners of two-dimensional Xe islands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01579739
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  • 7
    Electronic Resource
    Electronic Resource
    Detection of monolayer gratings of adsorbates by linear optical diffractions (1991)
    Springer
    Applied physics 52 (1991), S. 317-322 
    Details
    ISSN: 1432-0630
    Keywords: 78.65. -s ; 07.60. Hv ; 68.35. Fx
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract We report the detection of linear optical diffraction of a He-Ne laser probe beam from a monolayer grating of molecular adsorbates up to the fifth order with no electronic or electromagnetic enhancement. The advantages of using linear diffraction to probe surface diffusion of adsorbates are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00324772
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  • 8
    Electronic Resource
    Electronic Resource
    Surface electromagnetic radiation from a spatially modulated oscillating dipole layer (1994)
    Springer
    Applied physics 58 (1994), S. 615-618 
    Details
    ISSN: 1432-0630
    Keywords: 78.65.—s ; 07.60.Hv ; 68.35.Fx
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract We present the calculation of surface electromagnetic radiation to all orders from a spatially modulated, oscillating dipole layer sandwiched between two dielectric media. The results properly include the macroscopic local field factors. In specular reflection direction, we show that the total radiation is partitioned into a term which is the zeroth-order counterpart to the higher-order diffraction and a term related only to the properties of two adjoining bulk media. The results unify the calculations by Shen and Heinz and McIntyre [1, 6, 7]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00348174
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  • 9
    Electronic Resource
    Electronic Resource
    Raman spectroscopy of strained GeSi alloys deposited on Ge substrates (1996)
    Springer
    Applied physics 62 (1996), S. 387-390 
    Details
    ISSN: 1432-0630
    Keywords: PACS: 78.30. ; j; 61.55.Hg; 81.15.Gh
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract.  The Raman scattering method has been successfully used to investigate the properties of GeSi alloys deposited on Ge substrates in this paper. The effect of Si composition and strain in the GeSi alloy on the Raman shifts of Ge–Ge, Ge–Si and Si–Si phonon modes is studied. The relationship between them have been derived by the assumption that the Raman shifts is nearly linear with Si composition and strain in the GeSi alloys. The experimental data show reasonable agreement with the fits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01594238
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  • 10
    Electronic Resource
    Electronic Resource
    GNP1, the high-affinity glutamine permease of S. cerevisiae (1996)
    Springer
    Current genetics 30 (1996), S. 107-114 
    Details
    ISSN: 1432-0983
    Keywords: Key words  Yeast ; Glutamine permease ; *Membrane transport ; Nitrogen-regulated gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Glutamine uptake in S. cerevisiae is mediated by at least three transporters: high- and low-affinity glutamine permeases and the general amino-acid permease. We have isolated the gene encoding the high-affinity glutamine permease and named it GNP1. The amino-acid sequence of GNP1, and its hydropathy profile of 12 transmembrane domains, closely resemble those of known amino-acid permeases. The Km of GNP1 for glutamine uptake was determined to be 0.59 mM. Cells lacking GNP1 exhibit reduced levels of glutamine transport, and are resistant to a toxic analog of glutamine, L-glutamic acid γ-monohydroxamate. Unlike other amino-acid permeases, whose expression is nitrogen-source limited, GNP1 is expressed on both rich and poor nitrogen sources.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050108
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