GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Superoxide generation in transformed B-lymphocytes from patients with severe, malignant osteopetrosis (1999)
    Springer
    Molecular and cellular biochemistry 199 (1999), S. 15-24 
    Details
    ISSN: 1573-4919
    Keywords: B-lymphocytes ; osteopetrosis ; superoxide ; NADPH oxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Severe, malignant osteopetrosis is a disease characterized by osteoclasts that fail to resorb bone. Serious defects in the ability of white blood cells to eradicate infectious agents confound the clinical course. Defective superoxide generation by neutrophils, monocytes, and lymphocytes contributes to this inability to fight infection. To elucidate the mechanisms resulting in the defective superoxide generation observed in osteopetrotic leukocytes, gene expression, translocation, and phosphorylation of the major components that form the fimctional NADPH oxidase complex were studied in transformed B-lymphocytes. The expression of the p47 subunit of NADPH oxidase was reduced in B-lymphocytes collected from osteopetrotic patients compared to those from controls. Phosphorylation and translocation of p47 to the cell membrane after PMA stimulation was similar in B-lymphocytes from both patients and normal controls. However, total amount of p47 phosphorylation and translocation was reduced in patient samples. This was further supported by the experiment using p47 antisense oligonucleotide. The other major components of the oxidase (p91, p22, p67) were found to be present at normal levels. Thus, the reduction in p47 expression results in reduced ability to assemble a ftmctional NADPH oxidase complex at the membrane of lymphocytes from osteopetrotic patients. This defect translates into reduced superoxide generation and an increased propensity for infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006990730115
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    High-Pressure Effects on Vacuolar H+-ATPase from Etiolated Mung Bean Seedlings (1998)
    Springer
    The protein journal 17 (1998), S. 161-172 
    Details
    ISSN: 1573-4943
    Keywords: Tonoplast ; vacuolar H+-ATPase ; hydrostatic pressure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A high-hydrostatic-pressure technique was employed to study the structure-function relationship of plant vacuolar H+-ATPase from etiolated mung bean seedlings (Vigna radiata L.). When isolated vacuolar H+-ATPase was subjected to hydrostatic pressure, the activity of ATP hydrolysis was markedly inhibited in a time-, protein concentration- and pressure-dependent manner. The pressure treatment decreased both V max and K m of solubilized vacuolar H+-ATPase, implying an increase in ATP binding affinity, but a decrease in the ATP hydrolysis activity. Physiological substrate, Mg2+-ATP, augmented the loss of enzymatic activity upon pressure treatment. However, ADP, AMP, and Pi exerted substantial protective effects against pressurization. Steady-state ATP hydrolysis was more sensitive to pressurization than single-site ATPase activity. The inactivation of solubilized vacuolar H+-ATPase by pressure may result from changes in protein–protein interaction. The conformational change of solubilized vacuolar H+-ATPase induced by hydrostatic pressure was further determined by spectroscopic techniques. The inhibition of vacuolar H+-ATPase under pressurization involved at least two steps. Taken together, our work indicates that subunit–subunit interaction is crucial for the integrity and the function of plant vacuolar H+-ATPase. It is also suggested that the assembly of the vacuolar H+-ATPase complex is probably not random, but follows a sequestered pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022535701279
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Preparation and Adsorption Properties of Mesoporous Pillared Clays with Silica Sol (1998)
    Springer
    Journal of porous materials 5 (1998), S. 111-119 
    Details
    ISSN: 1573-4854
    Keywords: pillared clays ; intercalation ; microporosity ; mesoporosity ; organic template
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract New mesoporous silica-pillared montmorillonites were prepared by the post-intercalation of bulky organic cations into silica sol exchanged montmorillonites. Pillared clays with SiO2-Fe2O3 sol were first prepared, followed by the dissolution of iron oxide (or hydroxide) from the pillars with hydrochloric acid. A portion of the interlayered sol particles were then replaced with organic cations which led to a rearrangement of silica sol particles between the silicate layers. The removal of the organics by burning resulted in mesoporous sol pillared clays with an average pore size of more than 20 Å in diameter. The BET specific surface area increased to as high as 950 m2/g in these mesoporous pillared clays.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009685118854
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...