GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes (1981)
    Springer
    Chromosoma 82 (1981), S. 205-216 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Fragments from section 3 of the salivary gland X chromosome of D. melanogaster were dissected with a micromanipulator. The DNA was extracted, cut and ligated to a λ vector in a volume of a few nanoliters in an oil chamber monitored through a microscope. From about 10 pg of DNA we obtained 80 recombinant clones, a sample of which were analysed and shown to contain Drosophila DNA which hybridises in situ to the region of section 3 of the X chromosome. With this technique we can isolate clones from any desired region as small as 200 kb from the euchromatic arms of polytene chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286105
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Membrane lipid degradation is related to interictal cortical activity in a series of seizures (1994)
    Springer
    Metabolic brain disease 9 (1994), S. 161-170 
    Details
    ISSN: 1573-7365
    Keywords: Seizures ; membrane phospholipids ; phospholipases ; interictal activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Brain levels of free fatty acids (FFA) and diacylglycerols (DAG) rise rapidly with the onset of seizures, reflecting activation of phospholipases A2 (PLA2) and C (PLC), respectively. However, the ictal/interictal accumulation of FFA attenuates as recurrent seizures continue. To assess the role of neuronal activity in stimulating PLA2 and C, we compared FFA and DAG in rat cerebral cortex during recurrent ictal periods as a function of associated levels of interictal activity. Pentobarbital-anesthetized rats were paralyzed, ventilated with 30% O2 and subjected to periodic pentylenetetrazol seizures at intervals of 5 min. Animals were killed with focused-microwave irradiation during either the 3rd or 15th seizure. The rise in cortical FFA levels during early seizures for 20:4, 22:6, and 18:0 was 3.6-, 2.5-, and 2.2-fold greater, respectively, when adjacent interictal activity was intense as compared to weak activity. During late seizures, this difference dropped to 2.2-fold for 20:4, the only FFA that showed a significantly higher value between robust versus weak interictal activity. In contrast, accumulation of DAG during early and late seizures was observed only when adjacent interictal activity was high. These results indicate that the cortical accumulation of FFA and DAG during ictal periods of similar intensity and duration depends upon the electrocortical activity during adjacent interictal periods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01999769
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Cortical Impact Injury in Rats Promotes a Rapid and Sustained Increase in Polyunsaturated Free Fatty Acids and Diacylglycerols (2000)
    Springer
    Neurochemical research 25 (2000), S. 269-276 
    Details
    ISSN: 1573-6903
    Keywords: Arachidonic acid ; diacylglycerols ; docosahexaenoic acid ; free fatty acids ; phospholipase A2 ; phospholipase C ; traumatic brain injury
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Neurotrauma activates the release of membrane phospholipid-derived second messengers, such as free arachidonic acid (20:4n-6, AA) and diacylglycerols (DAGs). In the present study, we analyze the effect of cortical impact injury of low-grade severity applied to the rat frontal right sensory-motor cortex (FRC) on the accumulation of free fatty acids (FFAs) and DAGs in eight brain areas 30 min and 24 hours after the insult. At these times, accumulation of FFAs and DAGs occurred mainly in the damaged FRC. The cerebellum was the only other brain area that displayed a significant accumulation of DAGs by day one post-injury. By 30 min, accumulation of free AA in the FRC displayed the greatest relative increase (300% over sham value), followed by free docosahexaenoic acid (22:6n-3, DHA, 150%), while both 20:4-DAGs and 22:6-DAGs were increased 100% over sham values. At day one, free 22:6 and 22:6-DAGs showed the greatest increase (590% and 230%, respectively). These results suggest that TBI elicits the hydrolysis of phospholipids enriched in excitable membranes, targeting early on 20:4-phospholipids (by 30 min post-trauma) and followed 24 hours later by preferential hydrolysis of DHA-phospholipids. These lipid metabolic changes may contribute to the initiation and maturation of neuronal and fiber track degeneration observed following cortical impact injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007583806138
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...