ISSN:
1573-2592
Keywords:
B Cells
;
surface immunoglobulins
;
hypogammaglobulinemia
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract We investigated the prevalence of phenotypically immature IgG B cells (i.e., coexpressing surface IgG and IgM) in the peripheral blood of 12 patients with hypogammaglobulinemia and in normal individuals. Patients had ataxia-telangiectasia (N=1), hyper-IgM combined immunodeficiency (N=1), or common variable immunodeficiency (CVI). IgG/IgM-positive B cells were evaluated by two-color immunofluorescence using fluoresceinor rhodamine-conjugated goat antiserum; to minimize artifacts due toin vivo cytophilic binding of autologous IgG, cell-bound cytophilic Ig were eluted at pH 4 and Fc receptors were blocked by heat-aggregated rabbit IgG before fluorescent staining. All patients, except two with late-onset CVI, had markedly increased proportions of double-stained IgG B cells (56 to 100% of IgG-bearing B cells) in comparison with normal individuals (11 to 33%).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00915360
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