GLORIA

GEOMAR Library Ocean Research Information Access

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Allele specificity of theEscherichia coli dnaA gene function in the replication of plasmids derived from phageλ (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 580-586 
    Details
    ISSN: 1617-4623
    Keywords: Regulation of DNA replication ; Coliphageλ ; DnaA initiator ; Transcriptional activation ; Chaperone proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We demonstrate a variation in the effects of seven alleles of theEscherichia coli dnaA gene, which cause temperature sensitivity of initiation of chromosomal replication, on the replication ofλ phage-derived plasmids at 30° C. These mutants showed no allele specificity ofdnaA function in replication of either of twoλπ plasmids studied. On the other hand, the inability of theλP + plasmid to replicate indnaA508, 46 and204 cells, indnaB (groP A15) or in cells that are temperature sensitive for the chaperone genesdnaK756, dnaJ259 andgrpE280 and 30° C was suppressible by a singleπ mutation. This suggests that it is a common property of theπ protein, probably its weaker interaction with DnaB helicase, that is responsible for the suppression. One can also conclude that the DnaA-regulated transcriptional activation oforiλ acts at the step, in which all these gene products cooperate, i.e. during preprimosome loading and chaperone-mediated release of DnaB from P protein inhibition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172404
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The production of recombinant beta-galactosidase inEscherichia coli in yeast extract enriched medium (1990)
    Springer
    Journal of industrial microbiology and biotechnology 5 (1990), S. 85-93 
    Details
    ISSN: 1476-5535
    Keywords: Fermentation ; RecombinantE. coli ; Beta-galactosidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The productivity ofEscherichia coli biomass and recombinant beta-galactosidase was increased in Luria broth (LB) enriched with yeast extract. In flask culture under conditions of LB limitation, yeast extract suplementation gave the highest biomass (strain HB101/pRW756) stimulation per unit of component added compared with supplementation by various amounts of amino acids, vitamins, minerals, purines/pyrimidines, tryptone, casamino acids, casein peptone or gelatin peptone. The biomass production ofE. coli HB101/pRW756, XL-1 blue/puc118, XL-1 Blue FF/puc118 and TB-1/p1034 cells was stimulated in fermentor-scale experiments with additional yeast extract in LB. Total beta-galactosidase production from plasmid genes in fermentor-scale experiments was increased 105.4% in XL-1 blue/puc118 cells, 365.5% in XL-1 blue FF/puc118 cells and 421.4% in TB-1/p1034 cells by 0.5%, 1% and 1% weight per volume of additional yeast extract in LB, respectively. Depending on different strains, the increase of the enzyme production was obtained either by increased biomass, or the combination of enhanced gene expression and increased biomass. Neither the biomass nor beta-galactosidase production was stimulated in N4830/p1034 cells by the increase in yeast extract concentration in the medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01573857
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Intrinsic Faktor: Aktive und hemmende Komponenten aus den Mitochondrien von menschlichen Magen-Mucosazellen (1962)
    Springer
    Colloid & polymer science 183 (1962), S. 87-87 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02260580
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Katalytische Reduktion von Stickstoffoxid an Ruthenium (1977)
    Springer
    Colloid & polymer science 255 (1977), S. 814-814 
    Details
    ISSN: 1435-1536
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01664498
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Effects of centrophenoxine, piracetam and hydergine on rat brain lipid peroxidation (1981)
    Springer
    Cellular and molecular life sciences 37 (1981), S. 879-881 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cerebral insufficiency improvers centrophenoxine, piracetam and hydergine were tested for their effect on lipid peroxidation of rat brain homogenates, both in vitro and in vivo. There was no effect either in vitro or after chronic 8 week dosing of animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01985691
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Fetal gastric and colonic implants in syngeneic and allogeneic mice developing typical inflammatory changes (1983)
    Springer
    Cellular and molecular life sciences 39 (1983), S. 558-566 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Implants of fetal stomach and colon under the kidney capsule of syngeneic, and H-2 compatible and H-2 incompatible allogeneic mice were examined histologically at different time intervals after the procedure. According to the time of implantation typical inflammatory changes were seen in syngeneic stomach and colon implants, which resembled changes seen in chronic atrophic gastritis and chronic ulcerative colitis. Immunofluorescence studies showed that the host developed antibodies against fetal antigens, while there was no evidence for cellular immune response to fetal syngeneic antigens with the direct leukocyte migration inhibition test. Possible explanations for these results are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01971097
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Biological effects of singlet delta oxygen on respiratory tract epithelium (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 514-515 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Exposure of hamster tracheal organ cultures to gas phase singlet delta oxygen,1O2, at atmospheric pressure produced significant alterations in the mucociliary epithelium resulting in changes in ciliary activity and cellular morphology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01952414
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    The fermentation of xylose: studies by carbon-13 nuclear magnetic resonance spectroscopy (1990)
    Springer
    Journal of industrial microbiology and biotechnology 6 (1990), S. 29-41 
    Details
    ISSN: 1476-5535
    Keywords: Xylose ; Ethanol ; Fermentation ; NMR ; Pachysolen tannophilus ; Pichia stipitis ; Candida shehatae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The fermentation ofd-xylose byPachysolen tannophilus, Candida shehatae, andPichia stipitis has been investigated by13C-nuclear magnetic resonance spectroscopy of both whole cells and extracts. The spectra of whole cells metabolizingd-xylose with natural isotopic abundance had significant resonance signals corresponding only to xylitol, ethanol and xylose. The spectra of whole cells in the presence of [1-13C]xylose or [2-13C]xylose had resonance signals corresponding to the C-1 or C-2, respectively, of xylose, the C-1 or C-2, respectively, of xylitol, and the C-2 or C-1, respectively, of ethanol. Xylitol was metabolized only in the presence of an electron acceptor (acetone) and the only identifiable product was ethanol. The fact that the amount of ethanol was insufficient to account for the xylitol metabolized indicates that an additional fate of xylitol carbon must exist, probably carbon dioxide. The rapid metabolism of xylulose to ethanol, xylitol and arabinitol indicates that xylulose is a true intermediate and that xylitol dehydrogenase catalyzes the reduction (or oxidation) with different stereochemical specificity from that which interconverts xylitol andd-xylulose. The amino acidl-alanine was identified by the resonance position of the C-3 carbon and by enzymatic analysis of incubation mixtures containing yeast and [1-13C]xylose or [1-13C]glucose. The position of the label from both substrates and the identification of isotope also in C-1 of alamine indicates flux through the transketolase/transaldolase pathway in the metabolism. The identification of a resonance signal corresponding to the C-1 of ethanol in spectra of yeast in the presence of [1-13C]xylose and fluoroacetate (but not arsenite) indicates the existence of equilibration of some precursor of ethanol (e.g. pyruvate) with a symmetric intermediate (e.g. fumarate or succinate) under these conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01576174
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Biochemical and ultrastructural changes in A and B cells of the islets of Langerhans of mice infected with EMC virus (1979)
    Springer
    Diabetologia 17 (1979), S. 51-57 
    Details
    ISSN: 1432-0428
    Keywords: Diabetes ; EMC virus ; DBA2 mice ; islets of Langerhans ; ultrastructure ; insulin ; glucagon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Infection of DBA2 mice with the M strain of EMC virus was used to study the effects of virusinduced diabetes on the A and B cells of the islets of Langerhans. A transient hypoglycaemia was seen in 48% of mice 2–3 days after infection and probably resulted from increased serum insulin concentrations together with inhibition of glucagon secretion at that time. Islets from hypoglycaemic mice showed no significant alterations from control level in basal or fluoride-stimulated adenylate cyclase activity. Overall, 70% of infected mice became hyperglycaemic with a maximum incidence 6 days after infection. Hyperglycaemia was accompanied by a dramatic reduction in the total pancreatic insulin content and in insulin secretory responses to glucose and theophylline, while A-cell structure and function appeared relatively unaffected in diabetic animals. Basal adenylate cyclase activity was increased in hyperglycaemic mice at 7 days after infection, while fluoride-stimulated adenylate cyclase activity was normal throughout the course of infection. Ultrastructural alterations were observed in a small proportion of B cells from two days after infection and included abnormalities of mitochondrial structure and increased electron opacity of the cytoplasm of affected cells, which subsequently led to complete necrosis. The results suggest that EMC virus specifically affects the B cells of the islets and that disturbances of A cell function may be secondary to B cell damage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01222978
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    A prospective study of the effect of 12 months treatment on serum lipids and apolipoproteins A-I and B in type 2 (non-insulin-dependent) diabetes (1982)
    Springer
    Diabetologia 23 (1982), S. 507-510 
    Details
    ISSN: 1432-0428
    Keywords: Type 2 diabetes ; prospective study ; diet ; metformin ; glibenclamide ; HDL-cholesterol ; total cholesterol ; triglyceride ; apolipoproteins A-I and B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Serum high density lipoprotein cholesterol, total cholesterol, triglyceride and apolipoproteins A-I and B were studied at diagnosis and after 12 months conventional treatment in a group of Type 2 (non-insulin-dependent) diabetic patients. No significant deleterious effect on serum lipids and apolipoproteins was seen in any of the subgroups during the treatment period, although in the overall group there was a significant increase in serum total cholesterol in females. Serum high density lipoprotein cholesterol increased significantly in obese males treated with calorie restriction alone. There was a significant increase in serum apolipoprotein A-I in obese females treated with calorie restriction and metformin and in non-obese females treated with carbohydrate restriction and glibenclamide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00254300
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...