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  • 1
    Electronic Resource
    Electronic Resource
    Proline production via the arginine biosynthetic pathway: transfer of regulatory mutations of arginine biosynthesis into a proline-producing strain ofSerratia marcescens (1986)
    Springer
    Applied microbiology and biotechnology 24 (1986), S. 153-158 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Proline production via a part of the arginine biosynthetic pathway was examined. About 20 mg/ml ofl-proline was produced by using arginine biosynthetic enzymes. Accordingly, three mutations of arginine biosynthesis, namely, derepression of arginine biosynthetic enzymes (assigned byargR2), feedback inhibition-resistant N-acetylglutamate synthase (assigned byargA2) and defectiveness in N-acetylornithine aminotransferase (assigned byargD −) were introduced by three transductional crosses into a proline-producing strain which produced about 55 mg/ml ofl-proline. The constructed strain produced 62 mg/ml ofl-proline, although about 10 mg/ml ofl-arginine and 1 mg/ml of N-acetylglutamate-γ-semialdehyde were produced as by-products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01982560
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Proline production via the arginine biosynthetic pathway: transfer of regulatory mutations of arginine biosynthesis into a proline-producing strain ofSerratia marcescens (1986)
    Springer
    Applied microbiology and biotechnology 24 (1986), S. 153-158 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Proline production via a part of the arginine biosynthetic pathway was examined. About 20 mg/ml ofl-proline was produced by using arginine biosynthetic enzymes. Accordingly, three mutations of arginine biosynthesis, namely, derepression of arginine biosynthetic enzymes (assigned byargR2), feedback inhibition-resistant N-acetylglutamate synthase (assigned byargA2) and defectiveness in N-acetylornithine aminotransferase (assigned byargD −) were introduced by three transductional crosses into a proline-producing strain which produced about 55 mg/ml ofl-proline. The constructed strain produced 62 mg/ml ofl-proline, although about 10 mg/ml ofl-arginine and 1 mg/ml of N-acetylglutamate-γ-semialdehyde were produced as by-products.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00938788
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Ca2+ dynamics at the frog motor nerve terminal (2000)
    Springer
    Pflügers Archiv 440 (2000), S. 351-365 
    Details
    ISSN: 1432-2013
    Keywords: BAPTA Ca2+ transient Facilitation Fluorescent Ca2+ indicator Presynaptic terminal Residual calcium Short-term plasticity Transmitter release
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Rises in free [Ca2+]i in response to various tetanic stimuli (Ca2+ transient) in frog motor nerve terminals were measured by recording fluorescence changes of Ca2+ indicators and analyzed in relation to short-term synaptic plasticity. Ca2+ transients reached a plateau after 10–20 impulses at 100 Hz and decayed in a three-exponential manner, in which the fast component was predominant. The plateau and fast component of the Ca2+ transient were elevated infralinearly with an increase in tetanus frequency. Computer simulation showed that the Ca2+ transients estimated from fluorescence changes faithfully reflect the true changes in [Ca2+]i except for the initial 20 ms. A slow Ca2+ chelator, EGTA, loaded into the nerve terminal, decreased the magnitude of both the fast and slow components of facilitation of transmitter release and the time constant of the former. A fast Ca2+ chelator, BAPTA, decreased the magnitude of fast facilitation but slightly increased its time constant. These results suggest that Ca2+ transients in the frog motor nerve terminals are primarily caused by Ca2+ entry and are dissipated by three components, in which the rate of the fast component is equivalent to that of free Ca2+ diffusion. The residual Ca2+ in the nerve terminals after stimulation accounts for the fast component of facilitation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004240000278
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  • 4
    Electronic Resource
    Electronic Resource
    Changes in total body potassium during long-term thiazide treatment: Effects of potassium supplements on total body and serum potassium in hypertensive patients with thiazide treatment (1994)
    Springer
    The international journal of angiology 3 (1994), S. 142-147 
    Details
    ISSN: 1615-5939
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To evaluate the effects of long-term treatment with diuretics on potassium metabolism in hypertensive patients, serum potassium (serum K) and total body potassium (TBK) were determined in 66 hypertensive patients receiving long-term trichlormethiazide therapy and in 42 hypertensive control patients receiving no medication. No significant change in serum K was observed during sixty months of thiazide therapy. Serum K was, however, decreased significantly in patients receiving thiazides for more than 60 months. TBK did not change significantly during the first 24 months of thiazide therapy, but it significantly decreased at 24 to 60 months of thiazide treatment (P〈0.01). The effects of potassium supplementation on serum K and TBK during thiazide treatment were studied prospectively in 6 hypertensive patients. Serum K and TBK did not change by the administration of potassium chloride (16–24 mEq/day) and potassium gluconate (16–24 mEq/day), but spironolactone (75 mg/day) increased serum K and TBK during thiazide treatment (p〈0.05).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02014933
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  • 5
    Electronic Resource
    Electronic Resource
    A case with bronchogenic cyst diagnosed by reconstruction CT prior to surgery which showed cardiac abnormalities (1985)
    Springer
    Heart and vessels 1 (1985), S. 51-55 
    Details
    ISSN: 1615-2573
    Keywords: Bronchogenic cyst ; Atrial arrhythmia ; Reconstruction CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case report of a patient with bronchogenic cyst who presented with cardiogenic symptoms was described. The precise diagnosis was finally made after completing various diagnostic procedures which included reconstruction computed tomography. The patient, a 30-year-old man, was admitted to our hospital because of palpitation, chest pain and fever of three weeks duration. An electrocardiogram (ECG) revealed evidence suggestive of left atrial overloading and frequent atrial premature beats. A chest roentgenogram showed an abnormal shadow behind the right upper portion of the heart and a cross-sectional echocardiogram revealed abnormal echoes with ill-defined margins in the left atrium. Computed tomography (CT) revealed the presence of the left atrial tumor but left atriography suggested that it was extracardiac and compressing the left atrium. Reconstruction CT was therefore performed and the cyst was diagnosed as being in a subcarinal position. A mobile calcified mass was also demonstrated by the CT. An operation was performed and a cyst, 6×3 cm, was removed almost completely and a histopathological diagnosis of bronchogenic cyst was made. In this particular case, ECG abnormalities which suggest atrial damage and the diagnostic usefulness of reconstruction CT are stressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02066488
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  • 6
    Electronic Resource
    Electronic Resource
    Relationship between the pressure and diameter of the carotid artery in humans (2000)
    Springer
    Heart and vessels 15 (2000), S. 49-51 
    Details
    ISSN: 1615-2573
    Keywords: Key words Carotid artery ; Pressure ; Diameter ; Echo tracking ; Catheter-tipped micromanometer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of this study was to examine the assumption of similarity between pressure and diameter-change waveforms in humans. We measured carotid arterial pressure and diameter change, simultaneously, in six patients with heart disease. In all patients, the carotid arterial pressure–diameter relationship could, in practice, be regarded as being linear.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00007261
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