ISSN:
1432-072X
Keywords:
Bioluminescence
;
Marine bacteria
;
Autoinduction
;
Continuous culture
;
Chemostat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Several strains of four species of luminous marine bacteria were maintained in a chemostat at a constant dilution rate and a variety of steady state densities by carbon (glycerol) limitation in order to study the relationship between culture density and bioluminescence activity. In general, luminescence per cell was constant at high culture density, and decreased dramatically at low culture density. For Vibrio fischeri, luminescence decreased to nondectable levels when the culture was maintained at low density; such dark cells were stimulated to synthesize luciferase and became luminous within minutes when purified autoinducer was added to the chemostat. Two strains, Photobacterium phosphoreum NZ11D and Photobacterium leiognathi S1, did not show the decrease in light intensity at low culture density that was characteristic of all other strains tested; they appeared to be constitutive for bioluminescence.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00414701
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