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  • 1
    Electronic Resource
    Electronic Resource
    Protoplasts of Gelidium robustum (Rhodophyta) (1993)
    Springer
    Hydrobiologia 260-261 (1993), S. 421-427 
    Details
    ISSN: 1573-5117
    Keywords: protoplasts ; Gelidium robustum ; agarophyte ; Rhodophyta
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Viable protoplasts were isolated from apices of the agarophyte Gelidium robustum (Gardn.) Hollenb. & Abb. using a combination of commercial cell-wall degrading enzymes and extracellular wall-degrading enzymes isolated from a marine bacterium. The protoplasts were approximately 8–15 µm in diameter, liberated mainly from the surface cell layers and from cells at the distal ends of medullary filaments. The bacterial enzyme alone was not sufficient to liberate significant numbers of protoplasts. Maximum yield was 9 × 105 protoplasts/g tissue (wet wt.). Optimum osmolality occurred between 1750–1950 mOs kg−1; yield and viability were severely diminished at osmolalities less than 1350 mOs kg−1. Viability, as determined by flurorescein diacetate staining and Evans Blue exclusion 1 hr after removal from the enzyme solution, was approximately 80–95%. Roughly 80% of the cells did not show Calcofluor fluorescence, while 40% stained positively for the presence of sulfated polysaccharides. Cell wall regeneration was observed with inconsistent reproducibility, and no cell division was observed when the protoplasts were placed in culture medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00049051
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of carbenoxolone Na on acute and chronic gastric ulcer models in experimental animals (1976)
    Springer
    Digestive diseases and sciences 21 (1976), S. 618-625 
    Details
    ISSN: 1573-2568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Effects of carbenoxolone Na on acute or chronic types of gastric lesions or ulcer models produced in rats, guinea pigs, or dogs were studied. Carbenoxolone Na, given either orally or intraperitoneally, produced a significant inhibition of stress-induced gastric lesions in intact or in pylorus-ligated rats. Acetylsalicylic acid (ASA)-induced or serotonin-induced gastric lesions in rats were also inhibited significantly by pretreatment with the drug. However, carbenoxolone Na did not affect the development of Shay ulceration in rats even though the peptic activity in gastric juices was markedly reduced by the drug. Histamine-induced gastric lesions in guinea pigs were not prevented by pretreatment with carbenoxolone Na. Although carbenoxolone Na, given for 10–20 days, did not promote the healing of stress-induced gastric lesions and acetic acid gastric ulcers in rats, it significantly accelerated the healing of chronic gastric ulcer produced in dogs by 3 weeks' treatment. Carbenoxolone Na prevented the acid back-diffusion caused by ASA without any influence on Na+ efflux in pylorus-ligated rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01071954
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Sodium doped binary clusters II: Electron shell of InnNam and AunNam clusters (1993)
    Springer
    The European physical journal 26 (1993), S. 89-91 
    Details
    ISSN: 1434-6079
    Keywords: 36,40. +d ; 34.50. Lf
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Sodium doped binary clusters of indium and gold were produced by a laser vaporization method using two independent lasers. Photoionization efficiency spectra of these binary clusters were measured. The ionization potentials thus determined indicate the occurrence of the electron shell structure in these binary clusters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01425627
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    Paper (German National Licenses)
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