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  • Springer  (2)
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  • 1
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Nucleotide sequence of the genome terminal region 3′ to the capsid-readthrough cistron were compared for 10 PAV-like isolates of barley yellow dwarf virus (BYDV) from three continents. The sequenced region varied in length from 853 to 864 nucleotides and the extent of sequence homology among the isolates ranged from 84 to 99%. Sequence variations occur mainly in two locations, one in the ORF6 coding region and the other near the genome 3′ terminus. Sequence homology grouping reveals three genetically distinct clusters of PAV isolates (A, B and C). Cluster A consists of the Australian isolates, cluster B of one Canadian and three French isolates, and cluster C of the French isolate, RG. Dissimilarities with the corresponding genome-3′-terminal region of the BYDV-MAV serotype were greater than those observed between the PAV isolates alone. Comparison with the sequence of the 3′ untranslated region of soybean dwarf virus revealed two stretches of nucleotide similarity, suggesting a common ancestor. Study of the coding ability revealed that the ORF6 is present in all the sequenced PAV isolates but differs in size and deduced amino acids composition. However, the fact that the majority of nucleotide changes are restricted to the third base position of the ORF6-codons suggests that ORF6 codes for a functional protein.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-0653
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Zea mays L.). However, there have been no reports that the virus causes significant reductions in size or yield of irrigated maize. Trials to assess the effect of PAV on maize yields were conducted for 3 yr with two hybrids (`Déa' and `Nobilis') and two isolates of PAV (2t and L14). These two isolates differed in geographic origin and virulence on barley (Hordeum vulgare L.), but they multiplied similarly to wild isolates in leaves of maize hybrids. The virus was detected in maize leaves during the same period after inoculation in two out of three years. The reason for low levels of virus in leaves in the 1996 season may have been high temperatures (〉30°C) following inoculation. Under these conditions, the virus was restricted to the roots for long periods. This blocking of virus migration and/or foliar multiplication was accompanied by less reddening, and there was almost no decrease in grain yield. High early leaf infection of the virus had little effect on the vegetative development of the hybrids (〈10% reduction in plant height), but grain yield was between 15 and 20% lower for a production of 10 to 12 t ha−1. This drop in yield was due to fewer kernels of maize per ear, while the 1000 kernel mass was unaffected. The loss in yield was not affected by either the virus isolate or the maize hybrid, although the intensity of symptoms differed between hybrids. The data show that there are potential losses incurred by PAV on maize yield. Therefore, these findings justify the establishment of a breeding program.
    Type of Medium: Electronic Resource
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